line |
stmt |
bran |
cond |
sub |
pod |
time |
code |
1
|
|
|
|
|
|
|
# |
2
|
|
|
|
|
|
|
# BioPerl module for Bio::TreeIO::newick |
3
|
|
|
|
|
|
|
# |
4
|
|
|
|
|
|
|
# Please direct questions and support issues to |
5
|
|
|
|
|
|
|
# |
6
|
|
|
|
|
|
|
# Cared for by Jason Stajich |
7
|
|
|
|
|
|
|
# |
8
|
|
|
|
|
|
|
# Copyright Jason Stajich |
9
|
|
|
|
|
|
|
# |
10
|
|
|
|
|
|
|
# You may distribute this module under the same terms as perl itself |
11
|
|
|
|
|
|
|
|
12
|
|
|
|
|
|
|
# POD documentation - main docs before the code |
13
|
|
|
|
|
|
|
|
14
|
|
|
|
|
|
|
=head1 NAME |
15
|
|
|
|
|
|
|
|
16
|
|
|
|
|
|
|
Bio::TreeIO::newick |
17
|
|
|
|
|
|
|
|
18
|
|
|
|
|
|
|
=head1 SYNOPSIS |
19
|
|
|
|
|
|
|
|
20
|
|
|
|
|
|
|
# do not use this module directly |
21
|
|
|
|
|
|
|
use Bio::TreeIO; |
22
|
|
|
|
|
|
|
|
23
|
|
|
|
|
|
|
my $treeio = Bio::TreeIO->new(-format => 'newick', |
24
|
|
|
|
|
|
|
-file => 't/data/LOAD_Ccd1.dnd'); |
25
|
|
|
|
|
|
|
my $tree = $treeio->next_tree; |
26
|
|
|
|
|
|
|
|
27
|
|
|
|
|
|
|
=head1 DESCRIPTION |
28
|
|
|
|
|
|
|
|
29
|
|
|
|
|
|
|
This module handles parsing and writing of Newick/PHYLIP/New Hampshire format. |
30
|
|
|
|
|
|
|
|
31
|
|
|
|
|
|
|
=head1 FEEDBACK |
32
|
|
|
|
|
|
|
|
33
|
|
|
|
|
|
|
=head2 Mailing Lists |
34
|
|
|
|
|
|
|
|
35
|
|
|
|
|
|
|
User feedback is an integral part of the evolution of this and other |
36
|
|
|
|
|
|
|
Bioperl modules. Send your comments and suggestions preferably to the |
37
|
|
|
|
|
|
|
Bioperl mailing list. Your participation is much appreciated. |
38
|
|
|
|
|
|
|
|
39
|
|
|
|
|
|
|
bioperl-l@bioperl.org - General discussion |
40
|
|
|
|
|
|
|
http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
41
|
|
|
|
|
|
|
|
42
|
|
|
|
|
|
|
=head2 Support |
43
|
|
|
|
|
|
|
|
44
|
|
|
|
|
|
|
Please direct usage questions or support issues to the mailing list: |
45
|
|
|
|
|
|
|
|
46
|
|
|
|
|
|
|
I |
47
|
|
|
|
|
|
|
|
48
|
|
|
|
|
|
|
rather than to the module maintainer directly. Many experienced and |
49
|
|
|
|
|
|
|
reponsive experts will be able look at the problem and quickly |
50
|
|
|
|
|
|
|
address it. Please include a thorough description of the problem |
51
|
|
|
|
|
|
|
with code and data examples if at all possible. |
52
|
|
|
|
|
|
|
|
53
|
|
|
|
|
|
|
=head2 Reporting Bugs |
54
|
|
|
|
|
|
|
|
55
|
|
|
|
|
|
|
Report bugs to the Bioperl bug tracking system to help us keep track |
56
|
|
|
|
|
|
|
of the bugs and their resolution. Bug reports can be submitted via the |
57
|
|
|
|
|
|
|
web: |
58
|
|
|
|
|
|
|
|
59
|
|
|
|
|
|
|
https://github.com/bioperl/bioperl-live/issues |
60
|
|
|
|
|
|
|
|
61
|
|
|
|
|
|
|
=head1 AUTHOR - Jason Stajich |
62
|
|
|
|
|
|
|
|
63
|
|
|
|
|
|
|
Email jason-at-bioperl-dot-org |
64
|
|
|
|
|
|
|
|
65
|
|
|
|
|
|
|
=head1 APPENDIX |
66
|
|
|
|
|
|
|
|
67
|
|
|
|
|
|
|
The rest of the documentation details each of the object methods. |
68
|
|
|
|
|
|
|
Internal methods are usually preceded with a _ |
69
|
|
|
|
|
|
|
|
70
|
|
|
|
|
|
|
=cut |
71
|
|
|
|
|
|
|
|
72
|
|
|
|
|
|
|
# Let the code begin... |
73
|
|
|
|
|
|
|
|
74
|
|
|
|
|
|
|
package Bio::TreeIO::newick; |
75
|
16
|
|
|
16
|
|
64
|
use strict; |
|
16
|
|
|
|
|
20
|
|
|
16
|
|
|
|
|
426
|
|
76
|
|
|
|
|
|
|
|
77
|
16
|
|
|
16
|
|
54
|
use Bio::Event::EventGeneratorI; |
|
16
|
|
|
|
|
16
|
|
|
16
|
|
|
|
|
348
|
|
78
|
|
|
|
|
|
|
|
79
|
16
|
|
|
16
|
|
47
|
use base qw(Bio::TreeIO Bio::TreeIO::NewickParser); |
|
16
|
|
|
|
|
18
|
|
|
16
|
|
|
|
|
5825
|
|
80
|
|
|
|
|
|
|
|
81
|
|
|
|
|
|
|
=head2 new |
82
|
|
|
|
|
|
|
|
83
|
|
|
|
|
|
|
Title : new |
84
|
|
|
|
|
|
|
Args : -print_count => boolean default is false |
85
|
|
|
|
|
|
|
-bootstrap_style => set the bootstrap style (one of nobranchlength, |
86
|
|
|
|
|
|
|
molphy, traditional) |
87
|
|
|
|
|
|
|
-order_by => set the order by sort method |
88
|
|
|
|
|
|
|
|
89
|
|
|
|
|
|
|
See L |
90
|
|
|
|
|
|
|
|
91
|
|
|
|
|
|
|
=cut |
92
|
|
|
|
|
|
|
|
93
|
|
|
|
|
|
|
sub _initialize { |
94
|
680
|
|
|
680
|
|
548
|
my $self = shift; |
95
|
680
|
|
|
|
|
1450
|
$self->SUPER::_initialize(@_); |
96
|
680
|
|
|
|
|
1653
|
my ( $print_count ) = $self->_rearrange( |
97
|
|
|
|
|
|
|
[ |
98
|
|
|
|
|
|
|
qw(PRINT_COUNT) |
99
|
|
|
|
|
|
|
], |
100
|
|
|
|
|
|
|
@_ |
101
|
|
|
|
|
|
|
); |
102
|
680
|
|
50
|
|
|
2479
|
$self->print_tree_count( $print_count || 0 ); |
103
|
680
|
|
|
|
|
852
|
return; |
104
|
|
|
|
|
|
|
} |
105
|
|
|
|
|
|
|
|
106
|
|
|
|
|
|
|
=head2 next_tree |
107
|
|
|
|
|
|
|
|
108
|
|
|
|
|
|
|
Title : next_tree |
109
|
|
|
|
|
|
|
Usage : my $tree = $treeio->next_tree |
110
|
|
|
|
|
|
|
Function: Gets the next tree in the stream |
111
|
|
|
|
|
|
|
Returns : L |
112
|
|
|
|
|
|
|
Args : none |
113
|
|
|
|
|
|
|
|
114
|
|
|
|
|
|
|
=cut |
115
|
|
|
|
|
|
|
|
116
|
|
|
|
|
|
|
sub next_tree { |
117
|
155
|
|
|
155
|
1
|
1962
|
my ($self) = @_; |
118
|
155
|
|
|
|
|
537
|
local $/ = ";\n"; |
119
|
155
|
100
|
|
|
|
385
|
return unless $_ = $self->_readline; |
120
|
|
|
|
|
|
|
|
121
|
153
|
|
|
|
|
705
|
s/[\r\n]//gs; |
122
|
153
|
|
|
|
|
138
|
my $score; |
123
|
153
|
|
|
0
|
|
515
|
my $despace = sub { my $dirty = shift; $dirty =~ s/\s+//gs; return $dirty }; |
|
0
|
|
|
|
|
0
|
|
|
0
|
|
|
|
|
0
|
|
|
0
|
|
|
|
|
0
|
|
124
|
|
|
|
|
|
|
my $dequote = sub { |
125
|
0
|
|
|
0
|
|
0
|
my $dirty = shift; |
126
|
0
|
|
|
|
|
0
|
$dirty =~ s/^"?\s*(.+?)\s*"?$/$1/; |
127
|
0
|
|
|
|
|
0
|
return $dirty; |
128
|
153
|
|
|
|
|
304
|
}; |
129
|
153
|
|
|
|
|
279
|
s/([^"]*)(".+?")([^"]*)/$despace->($1) . $dequote->($2) . $despace->($3)/egsx; |
|
0
|
|
|
|
|
0
|
|
130
|
|
|
|
|
|
|
|
131
|
153
|
100
|
|
|
|
398
|
if (s/^\s*\[([^\]]+)\]//) { |
132
|
2
|
|
|
|
|
7
|
my $match = $1; |
133
|
2
|
|
|
|
|
6
|
$match =~ s/\s//g; |
134
|
2
|
|
|
|
|
5
|
$match =~ s/lh\=//; |
135
|
2
|
50
|
|
|
|
9
|
if ( $match =~ /([-\d\.+]+)/ ) { |
136
|
2
|
|
|
|
|
4
|
$score = $1; |
137
|
|
|
|
|
|
|
} |
138
|
|
|
|
|
|
|
} |
139
|
|
|
|
|
|
|
|
140
|
153
|
|
|
|
|
355
|
$self->_eventHandler->start_document; |
141
|
|
|
|
|
|
|
|
142
|
|
|
|
|
|
|
# Call the parse_newick method as defined in NewickParser.pm |
143
|
153
|
|
|
|
|
421
|
$self->parse_newick($_); |
144
|
|
|
|
|
|
|
|
145
|
153
|
|
|
|
|
286
|
my $tree = $self->_eventHandler->end_document; |
146
|
|
|
|
|
|
|
|
147
|
|
|
|
|
|
|
# Add the tree score afterwards if it exists. |
148
|
153
|
50
|
|
|
|
381
|
if (defined $tree) { |
149
|
153
|
|
|
|
|
338
|
$tree->score($score); |
150
|
153
|
|
|
|
|
1429
|
return $tree; |
151
|
|
|
|
|
|
|
} |
152
|
|
|
|
|
|
|
} |
153
|
|
|
|
|
|
|
|
154
|
|
|
|
|
|
|
# Returns the default set of parsing & writing parameters for the Newick format. |
155
|
|
|
|
|
|
|
sub get_default_params { |
156
|
680
|
|
|
680
|
0
|
606
|
my $self = shift; |
157
|
|
|
|
|
|
|
|
158
|
|
|
|
|
|
|
return { |
159
|
680
|
|
|
|
|
3102
|
newline_each_node => 0, |
160
|
|
|
|
|
|
|
order_by => '', # ??? |
161
|
|
|
|
|
|
|
bootstrap_style => 'traditional', # Can be 'traditional', 'molphy', 'nobranchlength' |
162
|
|
|
|
|
|
|
internal_node_id => 'id', # Can be 'id' or 'bootstrap' |
163
|
|
|
|
|
|
|
|
164
|
|
|
|
|
|
|
no_branch_lengths => 0, |
165
|
|
|
|
|
|
|
no_bootstrap_values => 0, |
166
|
|
|
|
|
|
|
no_internal_node_labels => 0 |
167
|
|
|
|
|
|
|
}; |
168
|
|
|
|
|
|
|
} |
169
|
|
|
|
|
|
|
|
170
|
|
|
|
|
|
|
|
171
|
|
|
|
|
|
|
=head2 write_tree |
172
|
|
|
|
|
|
|
|
173
|
|
|
|
|
|
|
Title : write_tree |
174
|
|
|
|
|
|
|
Usage : $treeio->write_tree($tree); |
175
|
|
|
|
|
|
|
Function: Write a tree out to data stream in newick/phylip format |
176
|
|
|
|
|
|
|
Returns : none |
177
|
|
|
|
|
|
|
Args : L object |
178
|
|
|
|
|
|
|
|
179
|
|
|
|
|
|
|
=cut |
180
|
|
|
|
|
|
|
|
181
|
|
|
|
|
|
|
sub write_tree { |
182
|
541
|
|
|
541
|
1
|
570
|
my ( $self, @trees ) = @_; |
183
|
541
|
50
|
|
|
|
688
|
if ( $self->print_tree_count ) { |
184
|
0
|
|
|
|
|
0
|
$self->_print( sprintf( " %d\n", scalar @trees ) ); |
185
|
|
|
|
|
|
|
} |
186
|
|
|
|
|
|
|
|
187
|
541
|
|
|
|
|
889
|
my $params = $self->get_params; |
188
|
|
|
|
|
|
|
|
189
|
541
|
|
|
|
|
652
|
foreach my $tree (@trees) { |
190
|
541
|
50
|
33
|
|
|
3709
|
if ( !defined $tree |
|
|
|
33
|
|
|
|
|
191
|
|
|
|
|
|
|
|| ref($tree) =~ /ARRAY/i |
192
|
|
|
|
|
|
|
|| !$tree->isa('Bio::Tree::TreeI') ) |
193
|
|
|
|
|
|
|
{ |
194
|
0
|
|
|
|
|
0
|
$self->throw( |
195
|
|
|
|
|
|
|
"Calling write_tree with non Bio::Tree::TreeI object\n"); |
196
|
|
|
|
|
|
|
} |
197
|
541
|
|
|
|
|
1085
|
my @data = $self->_write_tree_Helper( $tree->get_root_node, $params); |
198
|
541
|
|
|
|
|
2201
|
$self->_print( join( ',', @data ).";" ); |
199
|
|
|
|
|
|
|
} |
200
|
|
|
|
|
|
|
|
201
|
541
|
50
|
33
|
|
|
844
|
$self->flush if $self->_flush_on_write && defined $self->_fh; |
202
|
541
|
|
|
|
|
846
|
return; |
203
|
|
|
|
|
|
|
} |
204
|
|
|
|
|
|
|
|
205
|
|
|
|
|
|
|
sub _write_tree_Helper { |
206
|
3290
|
|
|
3290
|
|
2302
|
my $self = shift; |
207
|
3290
|
|
|
|
|
2433
|
my ( $node, $params ) = @_; |
208
|
3290
|
|
|
|
|
1921
|
my @data; |
209
|
|
|
|
|
|
|
|
210
|
3290
|
|
|
|
|
4737
|
foreach my $n ( $node->each_Descendent($params->{order_by}) ) { |
211
|
2749
|
|
|
|
|
3487
|
push @data, $self->_write_tree_Helper( $n, $params ); |
212
|
|
|
|
|
|
|
} |
213
|
|
|
|
|
|
|
|
214
|
3290
|
|
|
|
|
4472
|
my $label = $self->_node_as_string($node,$params); |
215
|
|
|
|
|
|
|
|
216
|
3290
|
100
|
|
|
|
3949
|
if ( scalar(@data) >= 1) { |
217
|
1355
|
|
|
|
|
1549
|
$data[0] = "(" . $data[0]; |
218
|
1355
|
|
|
|
|
1068
|
$data[-1] .= ")"; |
219
|
1355
|
|
|
|
|
1359
|
$data[-1] .= $label; |
220
|
|
|
|
|
|
|
} else { |
221
|
1935
|
|
|
|
|
1709
|
push @data, $label; |
222
|
|
|
|
|
|
|
} |
223
|
|
|
|
|
|
|
|
224
|
3290
|
|
|
|
|
5720
|
return @data; |
225
|
|
|
|
|
|
|
} |
226
|
|
|
|
|
|
|
|
227
|
|
|
|
|
|
|
sub _node_as_string { |
228
|
3290
|
|
|
3290
|
|
2230
|
my $self = shift; |
229
|
3290
|
|
|
|
|
1951
|
my $node = shift; |
230
|
3290
|
|
|
|
|
2029
|
my $params = shift; |
231
|
|
|
|
|
|
|
|
232
|
3290
|
|
|
|
|
2368
|
my $label_stringbuffer = ''; |
233
|
|
|
|
|
|
|
|
234
|
3290
|
100
|
66
|
|
|
6764
|
if ($params->{no_bootstrap_values} != 1 && |
|
|
50
|
100
|
|
|
|
|
|
|
|
66
|
|
|
|
|
|
|
|
100
|
|
|
|
|
235
|
|
|
|
|
|
|
!$node->is_Leaf && |
236
|
|
|
|
|
|
|
defined $node->bootstrap && |
237
|
|
|
|
|
|
|
$params->{bootstrap_style} eq 'traditional' && |
238
|
|
|
|
|
|
|
$params->{internal_node_id} eq 'bootstrap') { |
239
|
|
|
|
|
|
|
# If we're an internal node and we're using 'traditional' bootstrap style, |
240
|
|
|
|
|
|
|
# we output the bootstrap instead of any label. |
241
|
5
|
|
|
|
|
7
|
my $bootstrap = $node->bootstrap; |
242
|
5
|
50
|
|
|
|
7
|
$label_stringbuffer .= $bootstrap if (defined $bootstrap); |
243
|
|
|
|
|
|
|
} elsif ($params->{no_internal_node_labels} != 1) { |
244
|
3285
|
|
|
|
|
4050
|
my $id = $node->id; |
245
|
3285
|
100
|
|
|
|
5020
|
$label_stringbuffer .= $id if( defined $id ); |
246
|
|
|
|
|
|
|
} |
247
|
|
|
|
|
|
|
|
248
|
3290
|
50
|
|
|
|
4539
|
if ($params->{no_branch_lengths} != 1) { |
249
|
3290
|
|
|
|
|
4002
|
my $blen = $node->branch_length; |
250
|
3290
|
100
|
|
|
|
9288
|
$label_stringbuffer .= ":". $blen if (defined $blen); |
251
|
|
|
|
|
|
|
} |
252
|
|
|
|
|
|
|
|
253
|
3290
|
50
|
|
|
|
4249
|
if ($params->{bootstrap_style} eq 'molphy') { |
254
|
0
|
|
|
|
|
0
|
my $bootstrap = $node->bootstrap; |
255
|
0
|
0
|
|
|
|
0
|
$label_stringbuffer .= "[$bootstrap]" if (defined $bootstrap); |
256
|
|
|
|
|
|
|
} |
257
|
|
|
|
|
|
|
|
258
|
3290
|
50
|
|
|
|
3778
|
if ($params->{newline_each_node} == 1) { |
259
|
0
|
|
|
|
|
0
|
$label_stringbuffer .= "\n"; |
260
|
|
|
|
|
|
|
} |
261
|
|
|
|
|
|
|
|
262
|
3290
|
|
|
|
|
3516
|
return $label_stringbuffer; |
263
|
|
|
|
|
|
|
} |
264
|
|
|
|
|
|
|
|
265
|
|
|
|
|
|
|
|
266
|
|
|
|
|
|
|
=head2 print_tree_count |
267
|
|
|
|
|
|
|
|
268
|
|
|
|
|
|
|
Title : print_tree_count |
269
|
|
|
|
|
|
|
Usage : $obj->print_tree_count($newval) |
270
|
|
|
|
|
|
|
Function: Get/Set flag for printing out the tree count (paml,protml way) |
271
|
|
|
|
|
|
|
Returns : value of print_tree_count (a scalar) |
272
|
|
|
|
|
|
|
Args : on set, new value (a scalar or undef, optional) |
273
|
|
|
|
|
|
|
|
274
|
|
|
|
|
|
|
=cut |
275
|
|
|
|
|
|
|
|
276
|
|
|
|
|
|
|
sub print_tree_count { |
277
|
1221
|
|
|
1221
|
1
|
905
|
my $self = shift; |
278
|
1221
|
100
|
|
|
|
2317
|
return $self->{'_print_tree_count'} = shift if @_; |
279
|
541
|
|
50
|
|
|
1823
|
return $self->{'_print_tree_count'} || 0; |
280
|
|
|
|
|
|
|
} |
281
|
|
|
|
|
|
|
|
282
|
|
|
|
|
|
|
=head2 bootstrap_style |
283
|
|
|
|
|
|
|
|
284
|
|
|
|
|
|
|
Title : bootstrap_style |
285
|
|
|
|
|
|
|
Usage : $obj->bootstrap_style($newval) |
286
|
|
|
|
|
|
|
Function: A description of how bootstraps and branch lengths are |
287
|
|
|
|
|
|
|
written, as the ID part of the internal node or else in [] |
288
|
|
|
|
|
|
|
in the branch length (Molphy-like; I am sure there is a |
289
|
|
|
|
|
|
|
better name for this but am not sure where to go for some |
290
|
|
|
|
|
|
|
sort of format documentation) |
291
|
|
|
|
|
|
|
|
292
|
|
|
|
|
|
|
If no branch lengths are requested then no bootstraps are usually |
293
|
|
|
|
|
|
|
written (unless someone REALLY wants this functionality...) |
294
|
|
|
|
|
|
|
|
295
|
|
|
|
|
|
|
Can take on strings which contain the possible values of |
296
|
|
|
|
|
|
|
'nobranchlength' --> don't draw any branch lengths - this |
297
|
|
|
|
|
|
|
is helpful if you don't want to have to |
298
|
|
|
|
|
|
|
go through and delete branch len on all nodes |
299
|
|
|
|
|
|
|
'molphy' --> draw bootstraps (100) like |
300
|
|
|
|
|
|
|
(A:0.11,B:0.22):0.33[100]; |
301
|
|
|
|
|
|
|
'traditional' --> draw bootstraps (100) like |
302
|
|
|
|
|
|
|
(A:0.11,B:0.22)100:0.33; |
303
|
|
|
|
|
|
|
Returns : value of bootstrap_style (a scalar) |
304
|
|
|
|
|
|
|
Args : on set, new value (a scalar or undef, optional) |
305
|
|
|
|
|
|
|
|
306
|
|
|
|
|
|
|
=cut |
307
|
|
|
|
|
|
|
|
308
|
|
|
|
|
|
|
sub bootstrap_style { |
309
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
310
|
0
|
|
|
|
|
|
my $val = shift; |
311
|
0
|
0
|
|
|
|
|
if ( defined $val ) { |
312
|
|
|
|
|
|
|
|
313
|
0
|
0
|
|
|
|
|
if ( $val !~ /^nobranchlength|molphy|traditional/i ) { |
314
|
0
|
|
|
|
|
|
$self->warn( |
315
|
|
|
|
|
|
|
"requested an unknown bootstrap style $val, expect one of nobranchlength,molphy,traditional, not updating value.\n" |
316
|
|
|
|
|
|
|
); |
317
|
|
|
|
|
|
|
} |
318
|
|
|
|
|
|
|
else { |
319
|
0
|
|
|
|
|
|
$self->{'_bootstrap_style'} = $val; |
320
|
|
|
|
|
|
|
} |
321
|
|
|
|
|
|
|
} |
322
|
0
|
|
0
|
|
|
|
return $self->{'_bootstrap_style'} || 'traditional'; |
323
|
|
|
|
|
|
|
} |
324
|
|
|
|
|
|
|
|
325
|
|
|
|
|
|
|
=head2 order_by |
326
|
|
|
|
|
|
|
|
327
|
|
|
|
|
|
|
Title : order_by |
328
|
|
|
|
|
|
|
Usage : $obj->order_by($newval) |
329
|
|
|
|
|
|
|
Function: Allow node order to be specified (typically "alpha") |
330
|
|
|
|
|
|
|
See L |
331
|
|
|
|
|
|
|
Returns : value of order_by (a scalar) |
332
|
|
|
|
|
|
|
Args : on set, new value (a scalar or undef, optional) |
333
|
|
|
|
|
|
|
|
334
|
|
|
|
|
|
|
=cut |
335
|
|
|
|
|
|
|
|
336
|
|
|
|
|
|
|
sub order_by { |
337
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
338
|
|
|
|
|
|
|
|
339
|
0
|
0
|
|
|
|
|
return $self->{'order_by'} = shift if @_; |
340
|
0
|
|
|
|
|
|
return $self->{'order_by'}; |
341
|
|
|
|
|
|
|
} |
342
|
|
|
|
|
|
|
|
343
|
|
|
|
|
|
|
1; |