line |
true |
false |
branch |
237
|
0 |
15 |
unless (ref $self->{'seq'} and $self->{'seq'}->isa('Bio::SeqI')) |
245
|
2 |
28 |
if (ref $self->{$primer} and $self->{$primer}->isa('Bio::PrimarySeqI')) |
249
|
0 |
30 |
unless (ref $self->{$primer} and $self->{$primer}->isa('Bio::SeqFeature::Primer')) |
260
|
0 |
1 |
unless ($self->{'left'}->start and $self->{'left'}->end and $self->{'right'}->start and $self->{'right'}->end) |
285
|
2 |
10 |
if (not defined $arg) { } |
|
5 |
5 |
elsif ($arg =~ /^-?l/) { } |
|
3 |
2 |
elsif ($arg =~ /^-?r/) { } |
|
2 |
0 |
elsif ($arg =~ /^-?b/) { } |
389
|
0 |
14 |
unless (defined $before or not defined $middle || !defined($after)) |
390
|
0 |
0 |
unless ($target_seq =~ /$left_seq/) |
393
|
0 |
0 |
unless ($target_seq =~ /$right_seq/) |
410
|
0 |
14 |
if (exists $left->{'PRIMER_LEFT'} or exists $right->{'PRIMER_RIGHT'} or exists $self->{'PRIMER_PRODUCT_SIZE'}) |
422
|
0 |
0 |
if (defined $primer_left and not $primer_left eq $left_location) |
426
|
0 |
0 |
if (defined $primer_right and not $primer_right eq $right_location) |
430
|
0 |
0 |
if (defined $primer_product and not $primer_product eq $amplicon_size) |