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# BioPerl module for Bio::PopGen::IO::csv |
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# |
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# Please direct questions and support issues to |
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# |
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# Cared for by Jason Stajich |
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# |
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# Copyright Jason Stajich |
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# |
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# You may distribute this module under the same terms as perl itself |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::PopGen::IO::csv -Extract individual allele data from a CSV parser |
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=head1 SYNOPSIS |
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#Do not use directly, use through the Bio::PopGen::IO driver |
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use Bio::PopGen::IO; |
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my $io = Bio::PopGen::IO->new(-format => 'csv', |
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-file => 'data.csv'); |
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# Some IO might support reading in a population at a time |
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my @population; |
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while( my $ind = $io->next_individual ) { |
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push @population, $ind; |
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} |
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=head1 DESCRIPTION |
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This object will parse comma delimited format (CSV) or whatever |
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delimiter you specify. It currently doesn't handle the more complex |
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quote escaped CSV format. There are 3 initialization parameters, |
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the delimiter (-field_delimiter) [default ','], (-allele_delimiter) |
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[default ' ']. The third initialization parameter is a boolean |
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-no_header which specifies if there is no header line to read in. All lines starting with '#' will be skipped |
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When no_header is not specific the data is assumed to be of the following form. |
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Having a header line this |
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SAMPLE,MARKERNAME1,MARKERNAME2,... |
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and each data line having the form (diploid data) |
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SAMP1,101 102,100 90,a b |
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or for haploid data |
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SAMP1,101,100,a |
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=head1 FEEDBACK |
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=head2 Mailing Lists |
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User feedback is an integral part of the evolution of this and other |
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Bioperl modules. Send your comments and suggestions preferably to |
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the Bioperl mailing list. Your participation is much appreciated. |
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bioperl-l@bioperl.org - General discussion |
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http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
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=head2 Support |
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Please direct usage questions or support issues to the mailing list: |
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I |
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rather than to the module maintainer directly. Many experienced and |
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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=head2 Reporting Bugs |
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Report bugs to the Bioperl bug tracking system to help us keep track |
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of the bugs and their resolution. Bug reports can be submitted via |
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the web: |
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https://github.com/bioperl/bioperl-live/issues |
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81
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=head1 AUTHOR - Jason Stajich |
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Email jason-at-bioperl.org |
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=head1 CONTRIBUTORS |
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Matthew Hahn, matthew.hahn-at-duke.edu |
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=head1 APPENDIX |
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The rest of the documentation details each of the object methods. |
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Internal methods are usually preceded with a _ |
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=cut |
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# Let the code begin... |
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99
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100
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package Bio::PopGen::IO::csv; |
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1
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1
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use vars qw($FieldDelim $AlleleDelim $NoHeader); |
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use strict; |
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103
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($FieldDelim,$AlleleDelim,$NoHeader) =( ',', '\s+',0); |
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# Object preamble - inherits from Bio::Root::Root |
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108
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use Bio::PopGen::Individual; |
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110
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use Bio::PopGen::Population; |
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use Bio::PopGen::Genotype; |
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use base qw(Bio::PopGen::IO); |
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114
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115
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=head2 new |
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Title : new |
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Usage : my $obj = Bio::PopGen::IO::csv->new(); |
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Function: Builds a new Bio::PopGen::IO::csv object |
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Returns : an instance of Bio::PopGen::IO::csv |
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Args : [optional, these are the current defaults] |
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-field_delimiter => ',' |
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-allele_delimiter=> '\s+' |
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-no_header => 0, |
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127
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=cut |
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129
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sub _initialize { |
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5
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my($self, @args) = @_; |
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my ($fieldsep,$all_sep, |
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$noheader) = $self->_rearrange([qw(FIELD_DELIMITER |
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ALLELE_DELIMITER |
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NO_HEADER)],@args); |
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$self->flag('no_header', defined $noheader ? $noheader : $NoHeader); |
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$self->flag('field_delimiter',defined $fieldsep ? $fieldsep : $FieldDelim); |
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$self->flag('allele_delimiter',defined $all_sep ? $all_sep : $AlleleDelim); |
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$self->{'_header'} = undef; |
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return 1; |
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} |
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145
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=head2 flag |
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147
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Title : flag |
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Usage : $obj->flag($flagname,$newval) |
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Function: Get/Set the flag value |
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Returns : value of a flag (a boolean) |
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Args : A flag name, currently we expect |
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'no_header', 'field_delimiter', or 'allele_delimiter' |
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on set, new value (a boolean or undef, optional) |
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156
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=cut |
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sub flag{ |
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766
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766
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1
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my $self = shift; |
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766
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my $fieldname = shift; |
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return unless defined $fieldname; |
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return $self->{'_flag'}->{$fieldname} = shift if @_; |
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return $self->{'_flag'}->{$fieldname}; |
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} |
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167
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168
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=head2 next_individual |
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170
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Title : next_individual |
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Usage : my $ind = $popgenio->next_individual; |
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Function: Retrieve the next individual from a dataset |
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Returns : L object |
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Args : none |
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176
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177
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=cut |
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179
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sub next_individual{ |
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my ($self) = @_; |
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112
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while( defined( $_ = $self->_readline) ) { |
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60
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100
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100
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287
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next if( /^\s*\#/ || /^\s+$/ || ! length($_) ); |
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183
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58
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last; |
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184
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} |
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100
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return if ! defined $_; |
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87
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if( $self->flag('no_header') || |
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defined $self->{'_header'} ) { |
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188
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189
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#########new (allows field delim to be the same as the allele delim |
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190
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191
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50
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63
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my ($samp,@marker_results); |
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192
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193
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50
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50
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67
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if($self->flag('field_delimiter') ne $self->flag('allele_delimiter')){ |
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194
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195
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67
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($samp,@marker_results) = split($self->flag('field_delimiter'),$_); |
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} |
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197
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else{ |
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198
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199
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0
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0
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my $fielddelim = $self->flag('field_delimiter'); |
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200
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0
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0
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my $alleledelim = $self->flag('allele_delimiter'); |
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201
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202
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0
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0
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($samp) = /(^.+?)$fielddelim/; |
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203
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0
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0
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s/^.+?$fielddelim//; |
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204
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205
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0
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0
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(@marker_results) = /([\d|\w]+$alleledelim[\d|\w]+)/g; |
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206
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207
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} |
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208
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209
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#########end new |
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210
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211
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50
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103
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my $i = 1; |
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212
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50
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60
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foreach my $m ( @marker_results ) { |
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213
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492
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790
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$m =~ s/^\s+//; |
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214
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492
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695
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$m =~ s/\s+$//; |
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215
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492
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467
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my $markername; |
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216
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492
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50
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645
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if( defined $self->{'_header'} ) { |
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217
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492
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582
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$markername = $self->{'_header'}->[$i]; |
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218
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} else { |
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219
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0
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0
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$markername = "Marker$i"; |
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220
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} |
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221
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492
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1335
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$self->debug( "markername is $markername alleles are $m\n"); |
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222
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223
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492
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736
|
my @alleles = split($self->flag('allele_delimiter'), $m); |
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224
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225
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492
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1462
|
$m = Bio::PopGen::Genotype->new(-alleles => \@alleles, |
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226
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-marker_name => $markername, |
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227
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-individual_id=> $samp); |
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228
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492
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714
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$i++; |
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229
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} |
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230
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50
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|
144
|
return Bio::PopGen::Individual->new(-unique_id => $samp, |
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231
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|
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-genotypes => \@marker_results); |
|
232
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} else { |
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233
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3
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6
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chomp; |
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234
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3
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6
|
$self->{'_header'} = [split($self->flag('field_delimiter'),$_)]; |
|
235
|
3
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|
13
|
return $self->next_individual; # rerun loop again |
|
236
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|
} |
|
237
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0
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0
|
return; |
|
238
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|
|
} |
|
239
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|
240
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241
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|
=head2 next_population |
|
242
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|
243
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|
|
Title : next_population |
|
244
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|
|
Usage : my $ind = $popgenio->next_population; |
|
245
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|
|
Function: Retrieve the next population from a dataset |
|
246
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|
|
Returns : L object |
|
247
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|
|
Args : none |
|
248
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|
|
Note : Many implementation will not implement this |
|
249
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250
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|
=cut |
|
251
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|
252
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|
|
# Plan is to just return the whole dataset as a single population by |
|
253
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|
|
# default I think - people would then have each population in a separate |
|
254
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|
|
# file. |
|
255
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|
256
|
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|
|
sub next_population{ |
|
257
|
1
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|
|
1
|
1
|
7
|
my ($self) = @_; |
|
258
|
1
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|
1
|
my @inds; |
|
259
|
1
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|
6
|
while( my $ind = $self->next_individual ) { |
|
260
|
4
|
|
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|
|
11
|
push @inds, $ind; |
|
261
|
|
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|
|
} |
|
262
|
1
|
|
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|
|
9
|
Bio::PopGen::Population->new(-individuals => \@inds); |
|
263
|
|
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|
|
} |
|
264
|
|
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|
|
265
|
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|
|
266
|
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|
267
|
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|
268
|
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|
|
|
=head2 write_individual |
|
269
|
|
|
|
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|
|
|
|
270
|
|
|
|
|
|
|
Title : write_individual |
|
271
|
|
|
|
|
|
|
Usage : $popgenio->write_individual($ind); |
|
272
|
|
|
|
|
|
|
Function: Write an individual out in the file format |
|
273
|
|
|
|
|
|
|
Returns : none |
|
274
|
|
|
|
|
|
|
Args : L object(s) |
|
275
|
|
|
|
|
|
|
|
|
276
|
|
|
|
|
|
|
=cut |
|
277
|
|
|
|
|
|
|
|
|
278
|
|
|
|
|
|
|
sub write_individual{ |
|
279
|
1
|
|
|
1
|
1
|
9
|
my ($self,@inds) = @_; |
|
280
|
1
|
|
|
|
|
3
|
my $fielddelim = $self->flag('field_delimiter'); |
|
281
|
1
|
|
|
|
|
4
|
my $alleledelim= $self->flag('allele_delimiter'); |
|
282
|
|
|
|
|
|
|
|
|
283
|
1
|
|
|
|
|
3
|
foreach my $ind ( @inds ) { |
|
284
|
22
|
50
|
33
|
|
|
85
|
if (! ref($ind) || ! $ind->isa('Bio::PopGen::IndividualI') ) { |
|
285
|
0
|
|
|
|
|
0
|
$self->warn("Cannot write an object that is not a Bio::PopGen::IndividualI object ($ind)"); |
|
286
|
0
|
|
|
|
|
0
|
next; |
|
287
|
|
|
|
|
|
|
} |
|
288
|
|
|
|
|
|
|
# we'll go ahead and sort these until |
|
289
|
|
|
|
|
|
|
# we have a better way to insure a consistent order |
|
290
|
22
|
|
|
|
|
38
|
my @marker_names = sort $ind->get_marker_names; |
|
291
|
22
|
100
|
66
|
|
|
49
|
if( ! $self->flag('no_header') && |
|
292
|
|
|
|
|
|
|
! $self->flag('header_written') ) { |
|
293
|
1
|
|
|
|
|
14
|
$self->_print(join($fielddelim, ('SAMPLE', @marker_names)), "\n"); |
|
294
|
1
|
|
|
|
|
3
|
$self->flag('header_written',1); |
|
295
|
|
|
|
|
|
|
} |
|
296
|
|
|
|
|
|
|
$self->_print( join($fielddelim, $ind->unique_id, |
|
297
|
|
|
|
|
|
|
# we're chaining map here, pay attention and read |
|
298
|
|
|
|
|
|
|
# starting with the last map |
|
299
|
|
|
|
|
|
|
|
|
300
|
|
|
|
|
|
|
# we'll turn genotypes into allele pairs |
|
301
|
|
|
|
|
|
|
# which will be separated by the allele delimiter |
|
302
|
440
|
|
|
|
|
602
|
map { join($alleledelim,$_->get_Alleles) } |
|
303
|
|
|
|
|
|
|
# marker names will be sorted so we don't |
|
304
|
|
|
|
|
|
|
# have to worry about this between individuals |
|
305
|
|
|
|
|
|
|
# unless the individual set you pass in has |
|
306
|
|
|
|
|
|
|
# a mixed set of markers... |
|
307
|
|
|
|
|
|
|
# this will turn marker names into Genotypes |
|
308
|
22
|
|
|
|
|
37
|
map {$ind->get_Genotypes(-marker => $_)} |
|
|
440
|
|
|
|
|
703
|
|
|
309
|
|
|
|
|
|
|
@marker_names), "\n") |
|
310
|
|
|
|
|
|
|
} |
|
311
|
|
|
|
|
|
|
} |
|
312
|
|
|
|
|
|
|
|
|
313
|
|
|
|
|
|
|
=head2 write_population |
|
314
|
|
|
|
|
|
|
|
|
315
|
|
|
|
|
|
|
Title : write_population |
|
316
|
|
|
|
|
|
|
Usage : $popgenio->write_population($pop); |
|
317
|
|
|
|
|
|
|
Function: Write a population out in the file format |
|
318
|
|
|
|
|
|
|
Returns : none |
|
319
|
|
|
|
|
|
|
Args : L object(s) |
|
320
|
|
|
|
|
|
|
Note : Many implementation will not implement this |
|
321
|
|
|
|
|
|
|
|
|
322
|
|
|
|
|
|
|
=cut |
|
323
|
|
|
|
|
|
|
|
|
324
|
|
|
|
|
|
|
sub write_population{ |
|
325
|
1
|
|
|
1
|
1
|
8
|
my ($self,@pops) = @_; |
|
326
|
1
|
|
|
|
|
3
|
my $fielddelim = $self->flag('field_delimiter'); |
|
327
|
|
|
|
|
|
|
# my $alleledelim= $self->flag('allele_delimiter'); |
|
328
|
1
|
|
|
|
|
2
|
my $alleledelim = ' '; |
|
329
|
1
|
|
|
|
|
3
|
foreach my $pop ( @pops ) { |
|
330
|
2
|
50
|
33
|
|
|
18
|
if (! ref($pop) || ! $pop->isa('Bio::PopGen::PopulationI') ) { |
|
331
|
0
|
|
|
|
|
0
|
$self->warn("Cannot write an object that is not a Bio::PopGen::PopulationI object"); |
|
332
|
0
|
|
|
|
|
0
|
next; |
|
333
|
|
|
|
|
|
|
} |
|
334
|
|
|
|
|
|
|
# we'll go ahead and sort these until |
|
335
|
|
|
|
|
|
|
# we have a better way to insure a consistent order |
|
336
|
2
|
|
|
|
|
8
|
my @marker_names = sort $pop->get_marker_names; |
|
337
|
2
|
100
|
66
|
|
|
5
|
if( ! $self->flag('no_header') && |
|
338
|
|
|
|
|
|
|
! $self->flag('header_written') ) { |
|
339
|
1
|
|
|
|
|
7
|
$self->_print( join($fielddelim, ('SAMPLE', @marker_names)), |
|
340
|
|
|
|
|
|
|
"\n"); |
|
341
|
1
|
|
|
|
|
3
|
$self->flag('header_written',1); |
|
342
|
|
|
|
|
|
|
} |
|
343
|
2
|
|
|
|
|
5
|
foreach my $ind ( $pop->get_Individuals ) { |
|
344
|
|
|
|
|
|
|
$self->_print( join($fielddelim, $ind->unique_id, |
|
345
|
|
|
|
|
|
|
# we're chaining map here, pay attention |
|
346
|
|
|
|
|
|
|
# and read starting with the last map |
|
347
|
|
|
|
|
|
|
|
|
348
|
|
|
|
|
|
|
# we'll turn genotypes into allele pairs |
|
349
|
|
|
|
|
|
|
# which will be separated by the allele |
|
350
|
|
|
|
|
|
|
# delimiter |
|
351
|
340
|
|
|
|
|
500
|
map { join($alleledelim,$_->get_Alleles) } |
|
352
|
|
|
|
|
|
|
# marker names will be sorted so we don't |
|
353
|
|
|
|
|
|
|
# have to worry about this between individuals |
|
354
|
|
|
|
|
|
|
# unless the individual set you pass in has |
|
355
|
|
|
|
|
|
|
# a mixed set of markers... |
|
356
|
|
|
|
|
|
|
# this will turn marker names into Genotypes |
|
357
|
17
|
|
|
|
|
35
|
map {$ind->get_Genotypes(-marker => $_)} |
|
|
340
|
|
|
|
|
555
|
|
|
358
|
|
|
|
|
|
|
@marker_names), "\n"); |
|
359
|
|
|
|
|
|
|
} |
|
360
|
|
|
|
|
|
|
} |
|
361
|
|
|
|
|
|
|
} |
|
362
|
|
|
|
|
|
|
|
|
363
|
|
|
|
|
|
|
1; |