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# BioPerl module for Bio::ClusterIO.pm |
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# |
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# Please direct questions and support issues to |
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# |
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# Cared for by Andrew Macgregor |
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# |
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# Copyright Andrew Macgregor, Jo-Ann Stanton, David Green |
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# Molecular Embryology Group, Anatomy & Structural Biology, University of Otago |
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# http://anatomy.otago.ac.nz/meg |
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# |
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# You may distribute this module under the same terms as perl itself |
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# |
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# _history |
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# |
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# May 7, 2002 - changed from UniGene.pm to more generic ClusterIO.pm |
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# by Andrew Macgregor |
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# |
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# April 17, 2002 - Initial implementation by Andrew Macgregor |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::ClusterIO - Handler for Cluster Formats |
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=head1 SYNOPSIS |
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#NB: This example is unigene specific |
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use Bio::ClusterIO; |
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$stream = Bio::ClusterIO->new('-file' => "Hs.data", |
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'-format' => "unigene"); |
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# note: we quote -format to keep older perl's from complaining. |
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36
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while ( my $in = $stream->next_cluster() ) { |
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print $in->unigene_id() . "\n"; |
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while ( my $sequence = $in->next_seq() ) { |
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print $sequence->accession_number() . "\n"; |
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} |
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} |
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# Parsing errors are printed to STDERR. |
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=head1 DESCRIPTION |
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The ClusterIO module works with the ClusterIO format module to read |
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various cluster formats such as NCBI UniGene. |
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50
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=head1 CONSTRUCTORS |
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52
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=head2 Bio::ClusterIO-Enew() |
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54
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$str = Bio::ClusterIO->new(-file => 'filename', |
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-format=>$format); |
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57
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The new() class method constructs a new Bio::ClusterIO object. The |
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returned object can be used to retrieve or print cluster |
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objects. new() accepts the following parameters: |
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61
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=over 4 |
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63
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=item -file |
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65
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A file path to be opened for reading. |
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67
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=item -format |
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Specify the format of the file. Supported formats include: |
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71
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unigene *.data UniGene build files. |
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72
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dbsnp *.xml dbSNP XML files |
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73
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74
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If no format is specified and a filename is given, then the module |
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75
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will attempt to deduce it from the filename. If this is unsuccessful, |
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the main UniGene build format is assumed. |
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78
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The format name is case insensitive. 'UNIGENE', 'UniGene' and |
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79
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'unigene' are all supported, as are dbSNP, dbsnp, and DBSNP |
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80
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81
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=back |
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82
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83
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=head1 OBJECT METHODS |
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84
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85
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See below for more detailed summaries. The main methods are: |
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86
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87
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=head2 $cluster = $str-Enext_cluster() |
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88
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89
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Fetch the next cluster from the stream. |
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91
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92
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=head2 TIEHANDLE(), READLINE(), PRINT() |
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94
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These I've left in here because they were in the SeqIO |
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95
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module. Feedback appreciated. There they provide the tie interface. |
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See L for more details. |
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98
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=head1 FEEDBACK |
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100
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=head2 Mailing Lists |
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102
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User feedback is an integral part of the evolution of this |
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103
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and other Bioperl modules. Send your comments and suggestions preferably |
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to one of the Bioperl mailing lists. |
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105
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Your participation is much appreciated. |
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107
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bioperl-l@bioperl.org - General discussion |
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http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
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110
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=head2 Support |
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112
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Please direct usage questions or support issues to the mailing list: |
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113
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114
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I |
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116
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rather than to the module maintainer directly. Many experienced and |
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117
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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121
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=head2 Reporting Bugs |
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122
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123
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Report bugs to the Bioperl bug tracking system to help us keep track |
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the bugs and their resolution. Bug reports can be submitted via the |
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web: |
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127
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https://github.com/bioperl/bioperl-live/issues |
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129
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=head1 AUTHOR - Andrew Macgregor |
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131
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Email andrew@anatomy.otago.ac.nz |
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133
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=head1 APPENDIX |
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134
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135
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The rest of the documentation details each of the object |
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methods. Internal methods are usually preceded with a _ |
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137
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138
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=cut |
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139
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140
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#' |
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141
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# Let the code begin... |
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142
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143
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package Bio::ClusterIO; |
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144
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145
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2
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2
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use strict; |
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56
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146
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147
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148
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2
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2
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8
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use base qw(Bio::Root::Root Bio::Root::IO); |
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2
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2
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516
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149
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150
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151
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152
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=head2 new |
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153
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154
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Title : new |
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155
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Usage : Bio::ClusterIO->new(-file => $filename, -format => 'format') |
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Function: Returns a new cluster stream |
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157
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Returns : A Bio::ClusterIO::Handler initialised with the appropriate format |
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158
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Args : -file => $filename |
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159
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-format => format |
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160
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161
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=cut |
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162
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163
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164
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my $entry = 0; |
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165
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166
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sub new { |
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167
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4
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1
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my ($caller,@args) = @_; |
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168
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4
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33
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my $class = ref($caller) || $caller; |
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170
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# or do we want to call SUPER on an object if $caller is an |
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171
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# object? |
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4
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100
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if( $class =~ /Bio::ClusterIO::(\S+)/ ) { |
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2
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my ($self) = $class->SUPER::new(@args); |
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2
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9
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$self->_initialize(@args); |
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2
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return $self; |
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} else { |
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178
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2
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my %param = @args; |
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2
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8
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@param{ map { lc $_ } keys %param } = values %param; # lowercase keys |
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5
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13
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180
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my $format = $param{'-format'} || |
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2
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33
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8
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$class->_guess_format( $param{-file} || $ARGV[0] ); |
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2
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5
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$format = "\L$format"; # normalize capitalization to lower case |
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184
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2
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50
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6
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return unless( $class->_load_format_module($format) ); |
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2
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return "Bio::ClusterIO::$format"->new(@args); |
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} |
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187
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} |
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188
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189
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=head2 format |
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190
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191
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Title : format |
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192
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Usage : $format = $stream->format() |
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193
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Function: Get the cluster format |
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194
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Returns : cluster format |
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195
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Args : none |
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196
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197
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=cut |
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198
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199
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# format() method inherited from Bio::Root::IO |
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200
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201
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202
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# _initialize is chained for all ClusterIO classes |
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203
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204
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sub _initialize { |
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205
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2
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2
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7
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my($self, @args) = @_; |
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206
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# initialize the IO part |
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207
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2
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14
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$self->_initialize_io(@args); |
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208
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} |
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209
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210
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=head2 next_cluster |
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211
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212
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Title : next_cluster |
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213
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Usage : $cluster = $stream->next_cluster() |
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214
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Function: Reads the next cluster object from the stream and returns it. |
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215
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Returns : a L compliant object |
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216
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Args : none |
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217
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218
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219
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=cut |
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220
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221
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sub next_cluster { |
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0
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1
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0
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my ($self, $seq) = @_; |
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223
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0
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$self->throw("Sorry, you cannot read from a generic Bio::ClusterIO object."); |
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} |
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225
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226
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=head2 cluster_factory |
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227
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228
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Title : cluster_factory |
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Usage : $obj->cluster_factory($newval) |
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Function: Get/set the object factory to use for creating the cluster |
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objects. |
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Example : |
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Returns : a L compliant object |
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Args : on set, new value (a L |
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235
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compliant object or undef, optional) |
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236
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237
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238
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=cut |
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239
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240
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sub cluster_factory{ |
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5
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5
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1
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8
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my $self = shift; |
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243
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5
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100
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17
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return $self->{'cluster_factory'} = shift if @_; |
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4
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23
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return $self->{'cluster_factory'}; |
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} |
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246
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247
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=head2 object_factory |
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248
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249
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Title : object_factory |
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250
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Usage : $obj->object_factory($newval) |
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Function: This is an alias to cluster_factory with a more generic name. |
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252
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Example : |
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253
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Returns : a L compliant object |
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254
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Args : on set, new value (a L |
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255
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compliant object or undef, optional) |
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256
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257
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258
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=cut |
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259
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260
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sub object_factory{ |
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261
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0
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0
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1
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0
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return shift->cluster_factory(@_); |
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262
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} |
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263
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264
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=head2 _load_format_module |
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265
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266
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Title : _load_format_module |
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267
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Usage : *INTERNAL ClusterIO stuff* |
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268
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Function: Loads up (like use) a module at run time on demand |
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269
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Example : |
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270
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Returns : |
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271
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Args : |
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272
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273
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=cut |
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274
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|
275
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|
sub _load_format_module { |
|
276
|
2
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2
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|
6
|
my ($self,$format) = @_; |
|
277
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2
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5
|
my $module = "Bio::ClusterIO::" . $format; |
|
278
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2
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|
3
|
my $ok; |
|
279
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|
280
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2
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|
3
|
eval { |
|
281
|
2
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|
14
|
$ok = $self->_load_module($module); |
|
282
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|
|
}; |
|
283
|
2
|
50
|
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|
17
|
if ( $@ ) { |
|
284
|
0
|
|
|
|
|
0
|
print STDERR <
|
|
285
|
|
|
|
|
|
|
$self: could not load $format - for more details on supported formats please see the ClusterIO docs |
|
286
|
|
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|
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|
|
Exception $@ |
|
287
|
|
|
|
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|
|
END |
|
288
|
|
|
|
|
|
|
; |
|
289
|
|
|
|
|
|
|
} |
|
290
|
2
|
|
|
|
|
10
|
return $ok; |
|
291
|
|
|
|
|
|
|
} |
|
292
|
|
|
|
|
|
|
|
|
293
|
|
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|
|
|
|
=head2 _guess_format |
|
294
|
|
|
|
|
|
|
|
|
295
|
|
|
|
|
|
|
Title : _guess_format |
|
296
|
|
|
|
|
|
|
Usage : $obj->_guess_format($filename) |
|
297
|
|
|
|
|
|
|
Function: guess format based on file suffix |
|
298
|
|
|
|
|
|
|
Example : |
|
299
|
|
|
|
|
|
|
Returns : guessed format of filename (lower case) |
|
300
|
|
|
|
|
|
|
Args : |
|
301
|
|
|
|
|
|
|
Notes : formats that _filehandle() will guess include unigene and dbsnp |
|
302
|
|
|
|
|
|
|
|
|
303
|
|
|
|
|
|
|
=cut |
|
304
|
|
|
|
|
|
|
|
|
305
|
|
|
|
|
|
|
sub _guess_format { |
|
306
|
0
|
|
|
0
|
|
0
|
my $class = shift; |
|
307
|
0
|
0
|
|
|
|
0
|
return unless $_ = shift; |
|
308
|
0
|
0
|
|
|
|
0
|
return 'unigene' if /\.(data)$/i; |
|
309
|
0
|
0
|
|
|
|
0
|
return 'dbsnp' if /\.(xml)$/i; |
|
310
|
|
|
|
|
|
|
} |
|
311
|
|
|
|
|
|
|
|
|
312
|
|
|
|
|
|
|
sub DESTROY { |
|
313
|
1
|
|
|
1
|
|
2
|
my $self = shift; |
|
314
|
|
|
|
|
|
|
|
|
315
|
1
|
|
|
|
|
9
|
$self->close(); |
|
316
|
|
|
|
|
|
|
} |
|
317
|
|
|
|
|
|
|
|
|
318
|
|
|
|
|
|
|
# I need some direction on these!! The module works so I haven't fiddled with them! |
|
319
|
|
|
|
|
|
|
|
|
320
|
|
|
|
|
|
|
sub TIEHANDLE { |
|
321
|
0
|
|
|
0
|
|
|
my ($class,$val) = @_; |
|
322
|
0
|
|
|
|
|
|
return bless {'seqio' => $val}, $class; |
|
323
|
|
|
|
|
|
|
} |
|
324
|
|
|
|
|
|
|
|
|
325
|
|
|
|
|
|
|
sub READLINE { |
|
326
|
0
|
|
|
0
|
|
|
my $self = shift; |
|
327
|
0
|
0
|
0
|
|
|
|
return $self->{'seqio'}->next_seq() || undef unless wantarray; |
|
328
|
0
|
|
|
|
|
|
my (@list, $obj); |
|
329
|
0
|
|
|
|
|
|
push @list, $obj while $obj = $self->{'seqio'}->next_seq(); |
|
330
|
0
|
|
|
|
|
|
return @list; |
|
331
|
|
|
|
|
|
|
} |
|
332
|
|
|
|
|
|
|
|
|
333
|
|
|
|
|
|
|
sub PRINT { |
|
334
|
0
|
|
|
0
|
|
|
my $self = shift; |
|
335
|
0
|
|
|
|
|
|
$self->{'seqio'}->write_seq(@_); |
|
336
|
|
|
|
|
|
|
} |
|
337
|
|
|
|
|
|
|
|
|
338
|
|
|
|
|
|
|
1; |
|
339
|
|
|
|
|
|
|
|