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# |
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# BioPerl module for Bio::DB::InMemoryCache |
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# |
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# Please direct questions and support issues to |
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# |
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# Cared for by Ewan Birney |
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# |
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# Copyright Ewan Birney |
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# |
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# You may distribute this module under the same terms as perl itself |
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# |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::DB::InMemoryCache - Abstract interface for a sequence database |
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=head1 SYNOPSIS |
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$cachedb = Bio::DB::InMemoryCache->new( -seqdb => $real_db, |
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-number => 1000); |
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# |
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# get a database object somehow using a concrete class |
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# |
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$seq = $cachedb->get_Seq_by_id('ROA1_HUMAN'); |
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# |
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# $seq is a Bio::Seq object |
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# |
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=head1 DESCRIPTION |
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This is a memory cache system which saves the objects returned by |
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Bio::DB::RandomAccessI in memory to a hard limit of sequences. |
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=head1 CONTACT |
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Ewan Birney Ebirney@ebi.ac.ukE |
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=head2 Support |
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Please direct usage questions or support issues to the mailing list: |
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I |
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rather than to the module maintainer directly. Many experienced and |
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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=head2 Reporting Bugs |
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Report bugs to the Bioperl bug tracking system to help us keep track |
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the bugs and their resolution. Bug reports can be submitted via the |
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web: |
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https://github.com/bioperl/bioperl-live/issues |
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=head1 APPENDIX |
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62
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The rest of the documentation details each of the object |
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methods. Internal methods are usually preceded with a _ |
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=cut |
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67
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68
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# Let the code begin... |
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69
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70
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package Bio::DB::InMemoryCache; |
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72
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73
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149
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use strict; |
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3512
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74
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75
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149
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149
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39735
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use Bio::Seq; |
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149
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219
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149
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3343
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76
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77
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149
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149
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588
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use base qw(Bio::Root::Root Bio::DB::SeqI); |
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149
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184
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149
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45622
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78
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79
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sub new { |
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0
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1
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my ($class,@args) = @_; |
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81
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82
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0
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my $self = Bio::Root::Root->new(); |
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0
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bless $self,$class; |
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84
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85
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0
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my ($seqdb,$number,$agr) = |
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86
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$self->_rearrange([qw(SEQDB NUMBER AGRESSION)],@args); |
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87
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if( !defined $seqdb || !ref $seqdb || |
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0
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89
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!$seqdb->isa('Bio::DB::RandomAccessI') ) { |
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0
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$self->throw("Must be a RandomAccess database not a [$seqdb]"); |
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} |
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92
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93
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0
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0
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if( !defined $number ) { |
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0
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$number = 1000; |
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95
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} |
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96
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97
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0
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$self->seqdb($seqdb); |
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$self->number($number); |
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$self->agr($agr); |
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100
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101
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# we consider acc as the primary id here |
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102
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0
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$self->{'_cache_number_hash'} = {}; |
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103
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0
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$self->{'_cache_id_hash'} = {}; |
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104
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0
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$self->{'_cache_acc_hash'} = {}; |
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105
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0
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$self->{'_cache_number'} = 1; |
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106
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107
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0
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return $self; |
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108
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} |
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109
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110
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=head2 get_Seq_by_id |
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111
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112
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Title : get_Seq_by_id |
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113
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Usage : $seq = $db->get_Seq_by_id('ROA1_HUMAN') |
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114
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Function: Gets a Bio::Seq object by its name |
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115
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Returns : a Bio::Seq object |
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116
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Args : the id (as a string) of a sequence |
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117
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Throws : "id does not exist" exception |
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118
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119
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=cut |
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120
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121
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sub get_Seq_by_id{ |
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0
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0
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1
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my ($self,$id) = @_; |
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123
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124
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0
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0
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if( defined $self->{'_cache_id_hash'}->{$id} ) { |
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125
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0
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my $acc = $self->{'_cache_id_hash'}->{$id}; |
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126
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0
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my $seq = $self->{'_cache_acc_hash'}->{$acc}; |
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127
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$self->{'_cache_number_hash'}->{$seq->accession} = |
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0
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$self->{'_cache_number'}++; |
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0
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return $seq; |
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130
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} else { |
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0
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return $self->_load_Seq('id',$id); |
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} |
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133
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} |
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134
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135
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=head2 get_Seq_by_acc |
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136
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137
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Title : get_Seq_by_acc |
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138
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Usage : $seq = $db->get_Seq_by_acc('X77802'); |
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139
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Function: Gets a Bio::Seq object by accession number |
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140
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Returns : A Bio::Seq object |
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141
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Args : accession number (as a string) |
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142
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Throws : "acc does not exist" exception |
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143
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144
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=cut |
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145
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146
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sub get_Seq_by_acc{ |
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147
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0
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0
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1
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my ($self,$acc) = @_; |
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148
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149
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#print STDERR "In cache get for $acc\n"; |
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150
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0
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0
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if( defined $self->{'_cache_acc_hash'}->{$acc} ) { |
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151
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#print STDERR "Returning cached $acc\n"; |
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152
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0
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my $seq = $self->{'_cache_acc_hash'}->{$acc}; |
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153
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$self->{'_cache_number_hash'}->{$seq->accession} = |
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154
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0
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$self->{'_cache_number'}++; |
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155
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0
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return $seq; |
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156
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} else { |
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157
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0
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return $self->_load_Seq('acc',$acc); |
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158
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} |
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159
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} |
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160
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161
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162
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163
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sub number { |
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164
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0
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0
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0
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my ($self, $number) = @_; |
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165
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0
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0
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if ($number) { |
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166
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0
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$self->{'number'} = $number; |
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167
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} else { |
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168
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0
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return $self->{'number'}; |
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169
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} |
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170
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} |
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171
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172
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sub seqdb { |
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173
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0
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0
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0
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my ($self, $seqdb) = @_; |
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174
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0
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0
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if ($seqdb) { |
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175
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0
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$self->{'seqdb'} = $seqdb; |
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176
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} else { |
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177
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0
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return $self->{'seqdb'}; |
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178
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} |
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179
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} |
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180
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181
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sub agr { |
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182
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0
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0
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0
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my ($self, $agr) = @_; |
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183
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0
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0
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if ($agr) { |
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184
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0
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$self->{'agr'} = $agr; |
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185
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} else { |
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186
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0
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return $self->{'agr'}; |
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187
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} |
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188
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} |
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189
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190
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191
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sub _load_Seq { |
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192
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0
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0
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my ($self,$type,$id) = @_; |
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193
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194
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0
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my $seq; |
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195
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196
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0
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0
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if( $type eq 'id') { |
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0
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197
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0
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$seq = $self->seqdb->get_Seq_by_id($id); |
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198
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}elsif ( $type eq 'acc' ) { |
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199
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0
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$seq = $self->seqdb->get_Seq_by_acc($id); |
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200
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} else { |
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201
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0
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$self->throw("Bad internal error. Don't understand $type"); |
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202
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} |
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203
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0
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0
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if( ! $seq ) { |
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204
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# warding off bug #1628 |
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205
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0
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$self->debug("could not find seq $id in seqdb\n"); |
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206
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0
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return; |
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207
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} |
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208
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209
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0
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0
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if( $self->agr() ) { |
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210
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#print STDERR "Pulling out into memory\n"; |
|
211
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0
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my $newseq = Bio::Seq->new( -display_id => $seq->display_id, |
|
212
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-accession_number => $seq->accession, |
|
213
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-seq => $seq->seq, |
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214
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-desc => $seq->desc, |
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215
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); |
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216
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0
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0
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if( $self->agr() == 1 ) { |
|
217
|
0
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|
foreach my $sf ( $seq->top_SeqFeatures() ) { |
|
218
|
0
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|
$newseq->add_SeqFeature($sf); |
|
219
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|
} |
|
220
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221
|
0
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|
$newseq->annotation($seq->annotation); |
|
222
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|
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|
} |
|
223
|
0
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|
$seq = $newseq; |
|
224
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|
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|
|
} |
|
225
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|
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226
|
0
|
0
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|
|
|
if( $self->_number_free < 1 ) { |
|
227
|
|
|
|
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|
|
# remove the latest thing from the hash |
|
228
|
|
|
|
|
|
|
my @accs = sort { $self->{'_cache_number_hash'}->{$a} <=> |
|
229
|
0
|
|
|
|
|
|
$self->{'_cache_number_hash'}->{$b} } |
|
230
|
0
|
|
|
|
|
|
keys %{$self->{'_cache_number_hash'}}; |
|
|
0
|
|
|
|
|
|
|
|
231
|
|
|
|
|
|
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|
|
232
|
0
|
|
|
|
|
|
my $acc = shift @accs; |
|
233
|
|
|
|
|
|
|
# remove this guy |
|
234
|
0
|
|
|
|
|
|
my $seq = $self->{'_cache_acc_hash'}->{$acc}; |
|
235
|
|
|
|
|
|
|
|
|
236
|
0
|
|
|
|
|
|
delete $self->{'_cache_number_hash'}->{$acc}; |
|
237
|
0
|
|
|
|
|
|
delete $self->{'_cache_id_hash'}->{$seq->id}; |
|
238
|
0
|
|
|
|
|
|
delete $self->{'_cache_acc_hash'}->{$acc}; |
|
239
|
|
|
|
|
|
|
} |
|
240
|
|
|
|
|
|
|
|
|
241
|
|
|
|
|
|
|
# up the number, register this sequence into the hash. |
|
242
|
0
|
|
|
|
|
|
$self->{'_cache_id_hash'}->{$seq->id} = $seq->accession; |
|
243
|
0
|
|
|
|
|
|
$self->{'_cache_acc_hash'}->{$seq->accession} = $seq; |
|
244
|
0
|
|
|
|
|
|
$self->{'_cache_number_hash'}->{$seq->accession} = $self->{'_cache_number'}++; |
|
245
|
|
|
|
|
|
|
|
|
246
|
0
|
|
|
|
|
|
return $seq; |
|
247
|
|
|
|
|
|
|
} |
|
248
|
|
|
|
|
|
|
|
|
249
|
|
|
|
|
|
|
|
|
250
|
|
|
|
|
|
|
sub _number_free { |
|
251
|
0
|
|
|
0
|
|
|
my $self = shift; |
|
252
|
|
|
|
|
|
|
|
|
253
|
0
|
|
|
|
|
|
return $self->number - scalar(keys %{$self->{'_cache_number_hash'}}); |
|
|
0
|
|
|
|
|
|
|
|
254
|
|
|
|
|
|
|
} |
|
255
|
|
|
|
|
|
|
|
|
256
|
|
|
|
|
|
|
=head2 get_Seq_by_version |
|
257
|
|
|
|
|
|
|
|
|
258
|
|
|
|
|
|
|
Title : get_Seq_by_version |
|
259
|
|
|
|
|
|
|
Usage : $seq = $db->get_Seq_by_version('X77802.1'); |
|
260
|
|
|
|
|
|
|
Function: Gets a Bio::Seq object by sequence version |
|
261
|
|
|
|
|
|
|
Returns : A Bio::Seq object |
|
262
|
|
|
|
|
|
|
Args : accession.version (as a string) |
|
263
|
|
|
|
|
|
|
Throws : "acc.version does not exist" exception |
|
264
|
|
|
|
|
|
|
|
|
265
|
|
|
|
|
|
|
=cut |
|
266
|
|
|
|
|
|
|
|
|
267
|
|
|
|
|
|
|
sub get_Seq_by_version{ |
|
268
|
0
|
|
|
0
|
1
|
|
my ($self,@args) = @_; |
|
269
|
0
|
|
|
|
|
|
$self->throw("Not implemented it"); |
|
270
|
|
|
|
|
|
|
} |
|
271
|
|
|
|
|
|
|
|
|
272
|
|
|
|
|
|
|
## End of Package |
|
273
|
|
|
|
|
|
|
|
|
274
|
|
|
|
|
|
|
1; |