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# BioPerl module for Bio::SeqFeature::SimilarityPair |
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# |
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# Please direct questions and support issues to |
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# |
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# Cared for by Hilmar Lapp |
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# |
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# Copyright Hilmar Lapp |
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# |
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# You may distribute this module under the same terms as perl itself |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::SeqFeature::SimilarityPair - Sequence feature based on the similarity |
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of two sequences. |
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=head1 SYNOPSIS |
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$sim_pair = Bio::SeqFeature::SimilarityPair->from_searchResult($blastHit); |
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$sim = $sim_pair->query(); # a Bio::SeqFeature::Similarity object - the query |
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$sim = $sim_pair->hit(); # dto - the hit. |
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# some properties for the similarity pair |
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$expect = $sim_pair->significance(); |
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$score = $sim_pair->score(); |
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$bitscore = $sim_pair->bits(); |
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# this will not write the description for the sequence (only its name) |
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print $sim_pair->query()->gff_string(), "\n"; |
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=head1 DESCRIPTION |
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Lightweight similarity search result as a pair of Similarity |
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features. This class inherits off Bio::SeqFeature::FeaturePair and |
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therefore implements Bio::SeqFeatureI, whereas the two features of the |
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pair are descendants of Bio::SeqFeature::Generic, with better support |
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for representing similarity search results in a cleaner way. |
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=head1 FEEDBACK |
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=head2 Mailing Lists |
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User feedback is an integral part of the evolution of this and other |
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Bioperl modules. Send your comments and suggestions preferably to one |
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of the Bioperl mailing lists. Your participation is much appreciated. |
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bioperl-l@bioperl.org - General discussion |
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http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
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=head2 Support |
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Please direct usage questions or support issues to the mailing list: |
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I |
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rather than to the module maintainer directly. Many experienced and |
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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=head2 Reporting Bugs |
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Report bugs to the Bioperl bug tracking system to help us keep track |
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the bugs and their resolution. Bug reports can be submitted via the |
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web: |
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https://github.com/bioperl/bioperl-live/issues |
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=head1 AUTHOR - Hilmar Lapp |
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Email hlapp@gmx.net or hilmar.lapp@pharma.novartis.com |
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=head1 APPENDIX |
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The rest of the documentation details each of the object |
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methods. Internal methods are usually preceded with a _ |
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=cut |
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84
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# Let the code begin... |
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package Bio::SeqFeature::SimilarityPair; |
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use strict; |
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use Bio::SeqFeature::Similarity; |
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use Bio::Factory::ObjectFactory; |
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use base qw(Bio::SeqFeature::FeaturePair); |
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=head2 new |
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Title : new |
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Usage : my $similarityPair = Bio::SeqFeature::SimilarityPair->new |
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(-hit => $hit, |
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-query => $query, |
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-source => 'blastp'); |
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Function: Initializes a new SimilarityPair object |
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Returns : Bio::SeqFeature::SimilarityPair |
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Args : -query => The query in a Feature pair |
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-hit => (formerly '-subject') the subject/hit in a Feature pair |
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=cut |
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sub new { |
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my($class,@args) = @_; |
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if(! grep { lc($_) eq "-feature_factory"; } @args) { |
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114
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# if no overriding factory is provided, provide our preferred one |
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my $fact = Bio::Factory::ObjectFactory->new( |
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-type => "Bio::SeqFeature::Similarity", |
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-interface => "Bio::SeqFeatureI"); |
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push(@args, '-feature_factory', $fact); |
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} |
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my $self = $class->SUPER::new(@args); |
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my ($primary, $hit, $query, $fea1, $source,$sbjct) = |
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$self->_rearrange([qw(PRIMARY |
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HIT |
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QUERY |
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FEATURE1 |
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SOURCE |
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SUBJECT |
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)],@args); |
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if( $sbjct ) { |
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# undeprecated by Jason before 1.1 release |
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# $self->deprecated("use of -subject deprecated: SimilarityPair now uses 'hit'"); |
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if(! $hit) { $hit = $sbjct } |
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else { |
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$self->warn("-hit and -subject were specified, using -hit and ignoring -subject"); |
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} |
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} |
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# set the query and subject feature if provided |
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$self->query( $query) if $query && ! $fea1; |
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$hit && $self->hit($hit); |
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# the following refer to feature1, which is guaranteed to exist |
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if( defined $primary || ! defined $self->primary_tag) { |
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$primary = 'similarity' unless defined $primary; |
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$self->primary_tag($primary); |
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} |
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$source && $self->source_tag($source); |
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return $self; |
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} |
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# |
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# Everything else is just inherited from SeqFeature::FeaturePair. |
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# |
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159
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=head2 query |
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Title : query |
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Usage : $query_feature = $obj->query(); |
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$obj->query($query_feature); |
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Function: The query object for this similarity pair |
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Returns : Bio::SeqFeature::Similarity |
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Args : [optional] Bio::SeqFeature::Similarity |
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168
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See L, L |
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=cut |
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sub query { |
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5897
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return shift->feature1(@_); |
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} |
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=head2 subject |
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Title : subject |
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Usage : $sbjct_feature = $obj->subject(); |
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$obj->subject($sbjct_feature); |
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Function: Get/Set Subject for a SimilarityPair |
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Returns : Bio::SeqFeature::Similarity |
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Args : [optional] Bio::SeqFeature::Similarity |
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Notes : Deprecated. Use the method 'hit' instead |
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189
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=cut |
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191
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sub subject { |
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1
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my $self = shift; |
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# $self->deprecated("Method subject deprecated: use hit() instead"); |
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12
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$self->hit(@_); |
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} |
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197
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=head2 hit |
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198
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199
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Title : hit |
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200
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Usage : $sbjct_feature = $obj->hit(); |
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201
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$obj->hit($sbjct_feature); |
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Function: Get/Set Hit for a SimilarityPair |
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Returns : Bio::SeqFeature::Similarity |
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Args : [optional] Bio::SeqFeature::Similarity |
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205
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=cut |
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sub hit { |
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210
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4596
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4596
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1
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7008
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return shift->feature2(@_); |
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211
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} |
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212
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213
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=head2 source_tag |
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214
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215
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Title : source_tag |
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216
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Usage : $source = $obj->source_tag(); # i.e., program |
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217
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$obj->source_tag($evalue); |
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218
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Function: Gets the source tag (program name typically) for a feature |
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219
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Returns : string |
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220
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Args : [optional] string |
|
221
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222
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223
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=cut |
|
224
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225
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sub source_tag { |
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226
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70
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70
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1
|
91
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my ($self, @args) = @_; |
|
227
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228
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70
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100
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114
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if(@args) { |
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229
|
66
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|
94
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$self->hit()->source_tag(@args); |
|
230
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} |
|
231
|
70
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|
118
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return $self->query()->source_tag(@args); |
|
232
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} |
|
233
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234
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=head2 significance |
|
235
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|
236
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Title : significance |
|
237
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Usage : $evalue = $obj->significance(); |
|
238
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|
|
$obj->significance($evalue); |
|
239
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Function: |
|
240
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|
Returns : |
|
241
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Args : |
|
242
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|
243
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|
244
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|
|
=cut |
|
245
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|
246
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|
|
sub significance { |
|
247
|
0
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|
|
0
|
1
|
0
|
my ($self, @args) = @_; |
|
248
|
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|
249
|
0
|
0
|
|
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|
0
|
if(@args) { |
|
250
|
0
|
|
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|
0
|
$self->hit()->significance(@args); |
|
251
|
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|
|
} |
|
252
|
0
|
|
|
|
|
0
|
return $self->query()->significance(@args); |
|
253
|
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|
|
} |
|
254
|
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|
255
|
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|
|
|
=head2 score |
|
256
|
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|
257
|
|
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|
|
Title : score |
|
258
|
|
|
|
|
|
|
Usage : $score = $obj->score(); |
|
259
|
|
|
|
|
|
|
$obj->score($value); |
|
260
|
|
|
|
|
|
|
Function: |
|
261
|
|
|
|
|
|
|
Returns : |
|
262
|
|
|
|
|
|
|
Args : |
|
263
|
|
|
|
|
|
|
|
|
264
|
|
|
|
|
|
|
|
|
265
|
|
|
|
|
|
|
=cut |
|
266
|
|
|
|
|
|
|
|
|
267
|
|
|
|
|
|
|
sub score { |
|
268
|
231
|
|
|
231
|
1
|
292
|
my ($self, @args) = @_; |
|
269
|
|
|
|
|
|
|
|
|
270
|
231
|
100
|
|
|
|
443
|
if(@args) { |
|
271
|
18
|
|
|
|
|
21
|
$self->hit()->score(@args); |
|
272
|
|
|
|
|
|
|
} |
|
273
|
|
|
|
|
|
|
# Note: You might think it's only getting set on the hit object. |
|
274
|
|
|
|
|
|
|
# Actually, it's getting set on both hit and query. |
|
275
|
|
|
|
|
|
|
|
|
276
|
231
|
|
|
|
|
488
|
return $self->query()->score(@args); |
|
277
|
|
|
|
|
|
|
} |
|
278
|
|
|
|
|
|
|
|
|
279
|
|
|
|
|
|
|
=head2 bits |
|
280
|
|
|
|
|
|
|
|
|
281
|
|
|
|
|
|
|
Title : bits |
|
282
|
|
|
|
|
|
|
Usage : $bits = $obj->bits(); |
|
283
|
|
|
|
|
|
|
$obj->bits($value); |
|
284
|
|
|
|
|
|
|
Function: |
|
285
|
|
|
|
|
|
|
Returns : |
|
286
|
|
|
|
|
|
|
Args : |
|
287
|
|
|
|
|
|
|
|
|
288
|
|
|
|
|
|
|
|
|
289
|
|
|
|
|
|
|
=cut |
|
290
|
|
|
|
|
|
|
|
|
291
|
|
|
|
|
|
|
sub bits { |
|
292
|
200
|
|
|
200
|
1
|
566
|
my ($self, @args) = @_; |
|
293
|
|
|
|
|
|
|
|
|
294
|
200
|
50
|
|
|
|
364
|
if(@args) { |
|
295
|
0
|
|
|
|
|
0
|
$self->hit()->bits(@args); |
|
296
|
|
|
|
|
|
|
} |
|
297
|
200
|
|
|
|
|
469
|
return $self->query()->bits(@args); |
|
298
|
|
|
|
|
|
|
} |
|
299
|
|
|
|
|
|
|
|
|
300
|
|
|
|
|
|
|
################################################################# |
|
301
|
|
|
|
|
|
|
# aliases for backwards compatibility or convenience # |
|
302
|
|
|
|
|
|
|
################################################################# |
|
303
|
|
|
|
|
|
|
|
|
304
|
|
|
|
|
|
|
*sbjct = \&subject; |
|
305
|
|
|
|
|
|
|
|
|
306
|
|
|
|
|
|
|
1; |