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# |
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# BioPerl module for Bio::Search::Hit::HMMERHit |
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# |
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# Please direct questions and support issues to |
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# |
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# Cared for by Jason Stajich |
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# |
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# Copyright Jason Stajich |
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# |
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# You may distribute this module under the same terms as perl itself |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::Search::Hit::HMMERHit - A Hit module for HMMER hits |
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=head1 SYNOPSIS |
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use Bio::Search::Hit::HMMERHit; |
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my $hit = Bio::Search::Hit::HMMERHit->new(); |
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# use it in the same way as Bio::Search::Hit::GenericHit |
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=head1 DESCRIPTION |
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This is a specialization of L. There |
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are a few news methods L and L. Note that |
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L and L make no sense for this object and will |
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return 0. |
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=head1 FEEDBACK |
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=head2 Mailing Lists |
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User feedback is an integral part of the evolution of this and other |
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Bioperl modules. Send your comments and suggestions preferably to |
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the Bioperl mailing list. Your participation is much appreciated. |
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bioperl-l@bioperl.org - General discussion |
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http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
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=head2 Support |
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Please direct usage questions or support issues to the mailing list: |
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I |
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rather than to the module maintainer directly. Many experienced and |
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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=head2 Reporting Bugs |
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Report bugs to the Bioperl bug tracking system to help us keep track |
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of the bugs and their resolution. Bug reports can be submitted via the |
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web: |
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https://github.com/bioperl/bioperl-live/issues |
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=head1 AUTHOR - Jason Stajich |
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Email jason@bioperl.org |
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=head1 APPENDIX |
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The rest of the documentation details each of the object methods. |
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Internal methods are usually preceded with a _ |
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=cut |
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# Let the code begin... |
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package Bio::Search::Hit::HMMERHit; |
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use strict; |
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use base qw(Bio::Search::Hit::GenericHit); |
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=head2 new |
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Title : new |
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Usage : my $obj = Bio::Search::Hit::HMMERHit->new(); |
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Function: Builds a new Bio::Search::Hit::HMMERHit object |
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Returns : Bio::Search::Hit::HMMERHit |
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Args : |
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Plus the Bio::Search::Hit::GenericHit inherited params |
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-name => Name of Hit (required) |
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-description => Description (optional) |
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-accession => Accession number (optional) |
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-length => Length of the Hit (optional) |
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-score => Raw Score for the Hit (optional) |
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-significance => Significance value for the Hit (optional) |
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-algorithm => Algorithm used (BLASTP, FASTX, etc...) |
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-hsps => Array ref of HSPs for this Hit. |
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=cut |
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=head2 next_domain |
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Title : next_domain |
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Usage : my $domain = $hit->next_domain(); |
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Function: An alias for L, this will return the next HSP |
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Returns : L object |
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Args : none |
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=cut |
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sub next_domain{ shift->next_hsp } |
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=head2 domains |
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Title : domains |
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Usage : my @domains = $hit->domains(); |
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Function: An alias for L, this will return the full list of hsps |
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Returns : array of L objects |
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Args : none |
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=cut |
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sub domains{ shift->hsps() } |
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=head2 inherited Bio::Search::Hit::GenericHit methods |
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=cut |
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=head2 add_hsp |
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Title : add_hsp |
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Usage : $hit->add_hsp($hsp) |
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Function: Add a HSP to the collection of HSPs for a Hit |
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Returns : number of HSPs in the Hit |
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Args : Bio::Search::HSP::HSPI object |
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=cut |
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=head2 Bio::Search::Hit::HitI methods |
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=cut |
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=head2 name |
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Title : name |
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Usage : $hit_name = $hit->name(); |
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Function: returns the name of the Hit sequence |
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Returns : a scalar string |
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Args : [optional] scalar string to set the name |
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=cut |
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=head2 accession |
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Title : accession |
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Usage : $acc = $hit->accession(); |
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Function: Retrieve the accession (if available) for the hit |
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Returns : a scalar string (empty string if not set) |
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Args : none |
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=cut |
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=head2 description |
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Title : description |
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Usage : $desc = $hit->description(); |
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Function: Retrieve the description for the hit |
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Returns : a scalar string |
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Args : [optional] scalar string to set the descrition |
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=cut |
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=head2 length |
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Title : length |
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Usage : my $len = $hit->length |
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Function: Returns the length of the hit |
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Returns : integer |
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Args : [optional] integer to set the length |
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=cut |
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=head2 algorithm |
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192
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Title : algorithm |
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Usage : $alg = $hit->algorithm(); |
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Function: Gets the algorithm specification that was used to obtain the hit |
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For BLAST, the algorithm denotes what type of sequence was aligned |
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against what (BLASTN: dna-dna, BLASTP prt-prt, BLASTX translated |
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dna-prt, TBLASTN prt-translated dna, TBLASTX translated |
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dna-translated dna). |
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Returns : a scalar string |
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Args : [optional] scalar string to set the algorithm |
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=cut |
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=head2 raw_score |
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Title : raw_score |
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Usage : $score = $hit->raw_score(); |
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Function: Gets the "raw score" generated by the algorithm. What |
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this score is exactly will vary from algorithm to algorithm, |
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returning undef if unavailable. |
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Returns : a scalar value |
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Args : [optional] scalar value to set the raw score |
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=cut |
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=head2 significance |
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218
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Title : significance |
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Usage : $significance = $hit->significance(); |
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Function: Used to obtain the E or P value of a hit, i.e. the probability that |
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this particular hit was obtained purely by random chance. If |
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information is not available (nor calculatable from other |
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information sources), return undef. |
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Returns : a scalar value or undef if unavailable |
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225
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Args : [optional] scalar value to set the significance |
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226
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227
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=cut |
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228
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229
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=head2 bits |
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230
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231
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Usage : $hit_object->bits(); |
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232
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Purpose : Gets the bit score of the best HSP for the current hit. |
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233
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Example : $bits = $hit_object->bits(); |
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234
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Returns : Integer or undef if bit score is not set |
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235
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Argument : n/a |
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236
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237
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See Also : L |
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238
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239
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=cut |
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240
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241
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11
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11
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1
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41
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sub bits { return 0 } |
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242
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243
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=head2 next_hsp |
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244
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245
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Title : next_hsp |
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246
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Usage : while( $hsp = $obj->next_hsp()) { ... } |
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247
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Function : Returns the next available High Scoring Pair |
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248
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Example : |
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249
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Returns : Bio::Search::HSP::HSPI object or null if finished |
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250
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Args : none |
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251
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252
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=cut |
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253
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254
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=head2 hsps |
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255
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256
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Usage : $hit_object->hsps(); |
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257
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Purpose : Get a list containing all HSP objects. |
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258
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: Get the numbers of HSPs for the current hit. |
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259
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Example : @hsps = $hit_object->hsps(); |
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260
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: $num = $hit_object->hsps(); # alternatively, use num_hsps() |
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261
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Returns : Array context : list of Bio::Search::HSP::BlastHSP.pm objects. |
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262
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: Scalar context: integer (number of HSPs). |
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263
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: (Equivalent to num_hsps()). |
|
264
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Argument : n/a. Relies on wantarray |
|
265
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Throws : Exception if the HSPs have not been collected. |
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266
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267
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See Also : L, L |
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268
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269
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=cut |
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270
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271
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=head2 num_hsps |
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272
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273
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Usage : $hit_object->num_hsps(); |
|
274
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Purpose : Get the number of HSPs for the present Blast hit. |
|
275
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Example : $nhsps = $hit_object->num_hsps(); |
|
276
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Returns : Integer |
|
277
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Argument : n/a |
|
278
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Throws : Exception if the HSPs have not been collected. |
|
279
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280
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See Also : L |
|
281
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282
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=cut |
|
283
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284
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=head2 rewind |
|
285
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286
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Title : rewind |
|
287
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Usage : $hit->rewind; |
|
288
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Function: Allow one to reset the HSP iteration to the beginning |
|
289
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|
|
Since this is an in-memory implementation |
|
290
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|
Returns : none |
|
291
|
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|
Args : none |
|
292
|
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|
293
|
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|
=cut |
|
294
|
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|
295
|
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|
|
=head2 iteration |
|
296
|
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|
297
|
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|
|
Title : iteration |
|
298
|
|
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|
|
Usage : $obj->iteration($newval) |
|
299
|
|
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|
|
Function: PSI-BLAST iteration |
|
300
|
|
|
|
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|
|
Returns : value of iteration |
|
301
|
|
|
|
|
|
|
Args : newvalue (optional) |
|
302
|
|
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|
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|
303
|
|
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|
304
|
|
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|
|
=cut |
|
305
|
|
|
|
|
|
|
|
|
306
|
|
|
|
|
|
|
|
|
307
|
0
|
|
|
0
|
1
|
|
sub iteration { return 0 } |
|
308
|
|
|
|
|
|
|
|
|
309
|
|
|
|
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|
|
1; |