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=head1 NAME |
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Bio::Polloc::LocusIO - I/O interface of C<Bio::Polloc::Locus::*> objects |
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=head1 AUTHOR - Luis M. Rodriguez-R |
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Email lmrodriguezr at gmail dot com |
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=head1 IMPLEMENTS OR EXTENDS |
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=over |
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=item * |
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L<Bio::Polloc::Polloc::Root> |
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=item * |
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L<Bio::Polloc::Polloc::IO> |
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=back |
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=head1 SYNOPSIS |
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Read & write loci: |
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use strict; |
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use Bio::Polloc::LocusIO; |
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30
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my $locusI = Bio::Polloc::LocusIO->new(-file=>"t/loci.gff3", -format=>"gff3"); |
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my $locusO = Bio::Polloc::LocusIO->new(-file=>">out.gff3", -format=>"gff3"); |
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33
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while(my $locus = $locusI->next_locus){ |
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print "Got a ", $locus->type, " from ", $locus->from, " to ", $locus->to, "\n"; |
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# Filter per type |
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if($locus->type eq "repeat"){ |
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$locusO->write_locus($locus); |
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} |
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} |
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=cut |
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43
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package Bio::Polloc::LocusIO; |
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use strict; |
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80
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use base qw(Bio::Polloc::Polloc::Root Bio::Polloc::Polloc::IO); |
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3
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1731
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our $VERSION = 1.0503; # [a-version] from Bio::Polloc::Polloc::Version |
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48
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49
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=head1 PUBLIC METHODS |
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51
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Methods provided by the package |
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53
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=head2 new |
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55
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The basic initialization method |
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57
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=cut |
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59
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sub new { |
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1
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869
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my($caller,@args) = @_; |
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61
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4
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my $class = ref($caller) || $caller; |
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62
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63
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4
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50
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if($class !~ m/Bio::Polloc::LocusIO::(\S+)/){ |
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4
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my $bme = Bio::Polloc::Polloc::Root->new(@args); |
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65
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4
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my($format, $file) = $bme->_rearrange([qw(FORMAT FILE)], @args); |
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67
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4
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100
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($format = $file) =~ s/^.*\.// if $file and not $format; |
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4
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if($format){ |
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4
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$format = __PACKAGE__->_qualify_format($format); |
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4
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$class = "Bio::Polloc::LocusIO::" . $format if $format; |
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} |
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72
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} |
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73
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74
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4
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if($class =~ m/Bio::Polloc::LocusIO::(\S+)/){ |
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4
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7
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my $load = 0; |
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4
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if(__PACKAGE__->_load_module($class)){ |
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$load = $class; |
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} |
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80
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4
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if($load){ |
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81
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4
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my $self = $load->SUPER::new(@args); |
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82
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4
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32
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$self->debug("Got the LocusIO class $load"); |
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4
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$self->_initialize(@args); |
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84
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4
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return $self; |
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85
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86
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} |
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87
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88
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0
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0
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my $bme = Bio::Polloc::Polloc::Root->new(@args); |
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0
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0
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$bme->throw("Impossible to load the module", $class); |
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90
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} |
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0
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0
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my $bme = Bio::Polloc::Polloc::Root->new(@args); |
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0
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0
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$bme->throw("Impossible to load the proper Bio::Polloc::LocusI class with ". |
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"[".join("; ",@args)."]", $class); |
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} |
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95
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96
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=head2 format |
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98
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Gets/sets the format of the file |
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100
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=over |
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101
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102
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=item Arguments |
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104
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Format (str), currently supported: gff3. |
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105
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106
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=item Return |
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107
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108
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Format (str or C<undef>). |
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109
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110
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=back |
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111
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112
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=cut |
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113
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114
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sub format { |
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115
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0
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0
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1
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my($self,$value) = @_; |
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116
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0
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0
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0
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if($value){ |
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117
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0
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my $v = $self->_qualify_format($value); |
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0
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$self->throw("Attempting to set an invalid type of locus",$value) unless $v; |
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0
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$self->{'_format'} = $v; |
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120
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} |
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0
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return $self->{'_format'}; |
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} |
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124
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=head2 write_locus |
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125
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126
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Appends one locus to the output file. |
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127
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128
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=over |
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129
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130
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=item Arguments |
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131
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132
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=over |
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133
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134
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=item -locus I<Bio::Polloc::LocusI>, mandatory |
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135
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136
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The locus to append. |
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137
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138
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=item -force I<Bool (int)> |
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140
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If true, forces re-parsing of the locus. Otherwise, |
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141
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tries to load cached parsing (if any). |
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143
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=back |
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145
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=back |
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147
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=cut |
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148
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149
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sub write_locus { |
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150
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3
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3
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1
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9
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my($self, @args) = @_; |
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151
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3
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18
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my($locus) = $self->_rearrange([qw(LOCUS)], @args); |
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152
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3
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50
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$self->throw("You must provide the locus to append") unless defined $locus; |
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153
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3
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$self->throw("The obtained locus is not an object", $locus) |
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154
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unless UNIVERSAL::can($locus, 'isa'); |
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155
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3
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13
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$self->_write_locus_impl(@args); |
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156
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} |
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157
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158
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=head2 read_loci |
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159
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160
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Gets the loci stored in the input file. |
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161
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162
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=over |
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164
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=item Arguments |
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165
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166
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=over |
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168
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=item -genomes I<arrayref of Bio::Polloc::Genome objects> |
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169
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170
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An arrayref containing the L<Bio::Polloc::Genome> objects associated to |
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171
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the collection of loci. This is not mandatory, but C<seq> and |
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172
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C<genome> properties will not be set on the newly created objects |
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173
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if this parameter is not provided. |
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175
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=back |
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177
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=item Returns |
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179
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A L<Bio::Polloc::LociGroup> object. |
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180
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181
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=back |
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183
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=cut |
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184
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185
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3
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3
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1
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1108
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sub read_loci { return shift->_read_loci_impl(@_) } |
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186
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187
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=head2 next_locus |
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188
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189
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Reads the next locus in the buffer. |
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190
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191
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=over |
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192
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193
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=item Arguments |
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194
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195
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Same of L<read_loci> |
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196
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197
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=item Returns |
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198
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199
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A L<Bio::Polloc::LocusI> object. |
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200
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201
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=back |
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202
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203
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=cut |
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204
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205
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90
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90
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1
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270
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sub next_locus { return shift->_next_locus_impl(@_) } |
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206
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207
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=head1 INTERNAL METHODS |
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208
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209
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Methods intended to be used only within the scope of Bio::Polloc::* |
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210
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211
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=head2 _qualify_format |
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212
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213
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Uniformizes the distinct names that every format can receive |
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214
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215
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=over |
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216
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217
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=item Arguments |
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218
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219
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The requested format (str) |
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220
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221
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=item Returns |
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222
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223
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The qualified format (str or undef) |
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224
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225
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=back |
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226
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227
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=cut |
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228
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229
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sub _qualify_format { |
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230
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4
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4
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9
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my($self,$value) = @_; |
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231
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4
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50
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11
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return unless $value; |
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232
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4
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50
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29
|
$value = 'gff3' if $value =~ /^gff3?$/i; |
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233
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4
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12
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$value = lc $value; |
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234
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4
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11
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return $value; |
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235
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} |
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236
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237
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=head2 _write_locus_impl |
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238
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239
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|
Format-specific implementation of C<write_locus>. |
|
240
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241
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=cut |
|
242
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|
243
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|
|
sub _write_locus_impl { |
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244
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0
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0
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0
|
$_[0]->throw("_write_locus_impl", $_[0], 'Bio::Polloc::Polloc::UnimplementedException'); |
|
245
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} |
|
246
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247
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|
=head2 _read_loci_impl |
|
248
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|
249
|
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|
|
Format-specific implementation of C<next_locus>. |
|
250
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|
251
|
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|
=cut |
|
252
|
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|
253
|
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|
|
sub _read_loci_impl { |
|
254
|
3
|
|
|
3
|
|
7
|
my ($self,@args) = @_; |
|
255
|
3
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|
16
|
my($genomes) = $self->_rearrange([qw(GENOMES)], @args); |
|
256
|
3
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|
26
|
my $group = Bio::Polloc::LociGroup->new(-genomes=>$genomes); |
|
257
|
3
|
|
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|
17
|
while(my $locus = $self->next_locus(@args)){ |
|
258
|
87
|
|
|
|
|
306
|
$group->add_locus($locus); |
|
259
|
|
|
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|
|
} |
|
260
|
3
|
|
|
|
|
15
|
return $group; |
|
261
|
|
|
|
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|
|
} |
|
262
|
|
|
|
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|
|
263
|
|
|
|
|
|
|
=head2 _next_locus_impl |
|
264
|
|
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|
|
265
|
|
|
|
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|
|
=cut |
|
266
|
|
|
|
|
|
|
|
|
267
|
|
|
|
|
|
|
sub _next_locus_impl { |
|
268
|
0
|
|
|
0
|
|
0
|
$_[0]->throw("_next_locus_impl", $_[0], 'Bio::Polloc::Polloc::UnimplementedException'); |
|
269
|
|
|
|
|
|
|
} |
|
270
|
|
|
|
|
|
|
|
|
271
|
|
|
|
|
|
|
=head2 _save_locus |
|
272
|
|
|
|
|
|
|
|
|
273
|
|
|
|
|
|
|
=cut |
|
274
|
|
|
|
|
|
|
|
|
275
|
|
|
|
|
|
|
sub _save_locus { |
|
276
|
87
|
|
|
87
|
|
119
|
my($self, $locus) = @_; |
|
277
|
87
|
|
100
|
|
|
213
|
$self->{'_saved_loci'}||= []; |
|
278
|
87
|
50
|
|
|
|
166
|
push @{$self->{'_saved_loci'}}, $locus if defined $locus; |
|
|
87
|
|
|
|
|
174
|
|
|
279
|
87
|
|
|
|
|
628
|
return $locus; |
|
280
|
|
|
|
|
|
|
} |
|
281
|
|
|
|
|
|
|
|
|
282
|
|
|
|
|
|
|
=head2 _locus_by_id |
|
283
|
|
|
|
|
|
|
|
|
284
|
|
|
|
|
|
|
=cut |
|
285
|
|
|
|
|
|
|
|
|
286
|
|
|
|
|
|
|
sub _locus_by_id { |
|
287
|
68
|
|
|
68
|
|
105
|
my($self, $id) = @_; |
|
288
|
68
|
50
|
|
|
|
315
|
return unless defined $id; |
|
289
|
68
|
|
|
|
|
77
|
my @col = grep { $_->id eq $id } @{$self->{'_saved_loci'}}; |
|
|
4386
|
|
|
|
|
10283
|
|
|
|
68
|
|
|
|
|
732
|
|
|
290
|
68
|
|
|
|
|
499
|
return $col[0]; |
|
291
|
|
|
|
|
|
|
} |
|
292
|
|
|
|
|
|
|
|
|
293
|
|
|
|
|
|
|
=head2 _initialize |
|
294
|
|
|
|
|
|
|
|
|
295
|
|
|
|
|
|
|
=cut |
|
296
|
|
|
|
|
|
|
|
|
297
|
|
|
|
|
|
|
sub _initialize { |
|
298
|
4
|
|
|
4
|
|
8
|
my $self = shift; |
|
299
|
4
|
|
|
|
|
27
|
$self->_initialize_io(@_); |
|
300
|
|
|
|
|
|
|
} |
|
301
|
|
|
|
|
|
|
|
|
302
|
|
|
|
|
|
|
1; |