| line |
stmt |
bran |
cond |
sub |
pod |
time |
code |
|
1
|
|
|
|
|
|
|
=head1 NAME |
|
2
|
|
|
|
|
|
|
|
|
3
|
|
|
|
|
|
|
Bio::Polloc::Genome - A group of sequences from the same organism |
|
4
|
|
|
|
|
|
|
|
|
5
|
|
|
|
|
|
|
=head1 AUTHOR - Luis M. Rodriguez-R |
|
6
|
|
|
|
|
|
|
|
|
7
|
|
|
|
|
|
|
Email lmrodriguezr at gmail dot com |
|
8
|
|
|
|
|
|
|
|
|
9
|
|
|
|
|
|
|
=head1 LICENSE |
|
10
|
|
|
|
|
|
|
|
|
11
|
|
|
|
|
|
|
This package is licensed under the Artistic License - see LICENSE.txt |
|
12
|
|
|
|
|
|
|
|
|
13
|
|
|
|
|
|
|
=head1 IMPLEMENTS OR EXTENDS |
|
14
|
|
|
|
|
|
|
|
|
15
|
|
|
|
|
|
|
=over |
|
16
|
|
|
|
|
|
|
|
|
17
|
|
|
|
|
|
|
=item * |
|
18
|
|
|
|
|
|
|
|
|
19
|
|
|
|
|
|
|
L<Bio::Polloc::Polloc::Root> |
|
20
|
|
|
|
|
|
|
|
|
21
|
|
|
|
|
|
|
=back |
|
22
|
|
|
|
|
|
|
|
|
23
|
|
|
|
|
|
|
=cut |
|
24
|
|
|
|
|
|
|
|
|
25
|
|
|
|
|
|
|
package Bio::Polloc::Genome; |
|
26
|
4
|
|
|
4
|
|
4694
|
use strict; |
|
|
4
|
|
|
|
|
9
|
|
|
|
4
|
|
|
|
|
162
|
|
|
27
|
4
|
|
|
4
|
|
22
|
use base qw(Bio::Polloc::Polloc::Root); |
|
|
4
|
|
|
|
|
7
|
|
|
|
4
|
|
|
|
|
905
|
|
|
28
|
4
|
|
|
4
|
|
2728
|
use Bio::SeqIO; |
|
|
4
|
|
|
|
|
229177
|
|
|
|
4
|
|
|
|
|
2819
|
|
|
29
|
|
|
|
|
|
|
our $VERSION = 1.0503; # [a-version] from Bio::Polloc::Polloc::Version |
|
30
|
|
|
|
|
|
|
|
|
31
|
|
|
|
|
|
|
|
|
32
|
|
|
|
|
|
|
=head1 PUBLIC METHODS |
|
33
|
|
|
|
|
|
|
|
|
34
|
|
|
|
|
|
|
Methods provided by the package |
|
35
|
|
|
|
|
|
|
|
|
36
|
|
|
|
|
|
|
=head2 new |
|
37
|
|
|
|
|
|
|
|
|
38
|
|
|
|
|
|
|
=over |
|
39
|
|
|
|
|
|
|
|
|
40
|
|
|
|
|
|
|
=item |
|
41
|
|
|
|
|
|
|
|
|
42
|
|
|
|
|
|
|
The basic initialization method |
|
43
|
|
|
|
|
|
|
|
|
44
|
|
|
|
|
|
|
=item Arguments |
|
45
|
|
|
|
|
|
|
|
|
46
|
|
|
|
|
|
|
=over |
|
47
|
|
|
|
|
|
|
|
|
48
|
|
|
|
|
|
|
=item -name I<str> |
|
49
|
|
|
|
|
|
|
|
|
50
|
|
|
|
|
|
|
The name of the genome (from file if not provided). |
|
51
|
|
|
|
|
|
|
|
|
52
|
|
|
|
|
|
|
=item -file I<str> |
|
53
|
|
|
|
|
|
|
|
|
54
|
|
|
|
|
|
|
The file containing the (multi-)fasta with the genome. |
|
55
|
|
|
|
|
|
|
|
|
56
|
|
|
|
|
|
|
=back |
|
57
|
|
|
|
|
|
|
|
|
58
|
|
|
|
|
|
|
=back |
|
59
|
|
|
|
|
|
|
|
|
60
|
|
|
|
|
|
|
=cut |
|
61
|
|
|
|
|
|
|
|
|
62
|
|
|
|
|
|
|
sub new { |
|
63
|
5
|
|
|
5
|
1
|
812
|
my($caller,@args) = @_; |
|
64
|
5
|
|
|
|
|
44
|
my $self = $caller->SUPER::new(@args); |
|
65
|
5
|
|
|
|
|
18
|
$self->_initialize(@args); |
|
66
|
5
|
|
|
|
|
18
|
return $self; |
|
67
|
|
|
|
|
|
|
} |
|
68
|
|
|
|
|
|
|
|
|
69
|
|
|
|
|
|
|
=head2 build_set |
|
70
|
|
|
|
|
|
|
|
|
71
|
|
|
|
|
|
|
=over |
|
72
|
|
|
|
|
|
|
|
|
73
|
|
|
|
|
|
|
=item |
|
74
|
|
|
|
|
|
|
|
|
75
|
|
|
|
|
|
|
Builds a set of genomes. |
|
76
|
|
|
|
|
|
|
|
|
77
|
|
|
|
|
|
|
=item Arguments |
|
78
|
|
|
|
|
|
|
|
|
79
|
|
|
|
|
|
|
=over |
|
80
|
|
|
|
|
|
|
|
|
81
|
|
|
|
|
|
|
=item -files I<arrayref> |
|
82
|
|
|
|
|
|
|
|
|
83
|
|
|
|
|
|
|
An arrayref containing the files from which genomes must be build. |
|
84
|
|
|
|
|
|
|
|
|
85
|
|
|
|
|
|
|
=item -names I<arrayref> |
|
86
|
|
|
|
|
|
|
|
|
87
|
|
|
|
|
|
|
An arrayref containing the names of the genomes (in the same order |
|
88
|
|
|
|
|
|
|
of the files). |
|
89
|
|
|
|
|
|
|
|
|
90
|
|
|
|
|
|
|
=back |
|
91
|
|
|
|
|
|
|
|
|
92
|
|
|
|
|
|
|
=back |
|
93
|
|
|
|
|
|
|
|
|
94
|
|
|
|
|
|
|
=cut |
|
95
|
|
|
|
|
|
|
|
|
96
|
|
|
|
|
|
|
sub build_set { |
|
97
|
0
|
|
|
0
|
1
|
0
|
my($self, @args) = @_; |
|
98
|
0
|
|
|
|
|
0
|
my($files, $names) = $self->_rearrange([qw(FILES NAMES)], @args); |
|
99
|
0
|
0
|
|
|
|
0
|
return unless defined $files; |
|
100
|
0
|
|
0
|
|
|
0
|
$names||= []; |
|
101
|
0
|
|
|
|
|
0
|
my $out = []; |
|
102
|
0
|
|
|
|
|
0
|
for my $i (0 .. $#$files){ |
|
103
|
0
|
|
|
|
|
0
|
push @$out, Bio::Polloc::Genome->new(-file=>$files->[$i], -name=>$names->[$i]); |
|
104
|
|
|
|
|
|
|
} |
|
105
|
0
|
|
|
|
|
0
|
return $out; |
|
106
|
|
|
|
|
|
|
} |
|
107
|
|
|
|
|
|
|
|
|
108
|
|
|
|
|
|
|
=head2 file |
|
109
|
|
|
|
|
|
|
|
|
110
|
|
|
|
|
|
|
=over |
|
111
|
|
|
|
|
|
|
|
|
112
|
|
|
|
|
|
|
=item |
|
113
|
|
|
|
|
|
|
|
|
114
|
|
|
|
|
|
|
Sets/gets the file containing the genome |
|
115
|
|
|
|
|
|
|
|
|
116
|
|
|
|
|
|
|
=back |
|
117
|
|
|
|
|
|
|
|
|
118
|
|
|
|
|
|
|
=cut |
|
119
|
|
|
|
|
|
|
|
|
120
|
|
|
|
|
|
|
sub file { |
|
121
|
14
|
|
|
14
|
1
|
968
|
my($self, $value) = @_; |
|
122
|
14
|
100
|
|
|
|
50
|
if(defined $value){ |
|
123
|
5
|
|
|
|
|
13
|
$self->{'_file'} = $value; |
|
124
|
5
|
|
|
|
|
10
|
$self->{'_sequences'} = undef; |
|
125
|
|
|
|
|
|
|
} |
|
126
|
14
|
|
|
|
|
45
|
return $self->{'_file'}; |
|
127
|
|
|
|
|
|
|
} |
|
128
|
|
|
|
|
|
|
|
|
129
|
|
|
|
|
|
|
=head2 get_sequences |
|
130
|
|
|
|
|
|
|
|
|
131
|
|
|
|
|
|
|
=over |
|
132
|
|
|
|
|
|
|
|
|
133
|
|
|
|
|
|
|
=item |
|
134
|
|
|
|
|
|
|
|
|
135
|
|
|
|
|
|
|
Gets the collection of sequences. |
|
136
|
|
|
|
|
|
|
|
|
137
|
|
|
|
|
|
|
=item Returns |
|
138
|
|
|
|
|
|
|
|
|
139
|
|
|
|
|
|
|
An array of L<Bio::Seq> objects. |
|
140
|
|
|
|
|
|
|
|
|
141
|
|
|
|
|
|
|
=back |
|
142
|
|
|
|
|
|
|
|
|
143
|
|
|
|
|
|
|
=cut |
|
144
|
|
|
|
|
|
|
|
|
145
|
|
|
|
|
|
|
sub get_sequences { |
|
146
|
8
|
|
|
8
|
1
|
1733
|
my $self = shift; |
|
147
|
8
|
100
|
|
|
|
47
|
return $self->{'_sequences'} if defined $self->{'_sequences'}; |
|
148
|
2
|
|
|
|
|
5
|
$self->{'_sequences'} = []; |
|
149
|
2
|
|
|
|
|
8
|
my $seqIO = Bio::SeqIO->new(-file=>$self->file, -format=>'Fasta'); |
|
150
|
2
|
|
|
|
|
53710
|
while(my $seq = $seqIO->next_seq){ |
|
151
|
8
|
|
|
|
|
24684
|
push @{ $self->{'_sequences'} }, $seq; |
|
|
8
|
|
|
|
|
45
|
|
|
152
|
|
|
|
|
|
|
} |
|
153
|
2
|
50
|
|
|
|
84
|
return wantarray ? @{$self->{'_sequences'}} : $self->{'_sequences'}; |
|
|
0
|
|
|
|
|
0
|
|
|
154
|
|
|
|
|
|
|
} |
|
155
|
|
|
|
|
|
|
|
|
156
|
|
|
|
|
|
|
=head2 search_sequence |
|
157
|
|
|
|
|
|
|
|
|
158
|
|
|
|
|
|
|
=over |
|
159
|
|
|
|
|
|
|
|
|
160
|
|
|
|
|
|
|
=item |
|
161
|
|
|
|
|
|
|
|
|
162
|
|
|
|
|
|
|
Search a sequence by ID |
|
163
|
|
|
|
|
|
|
|
|
164
|
|
|
|
|
|
|
=item Arguments |
|
165
|
|
|
|
|
|
|
|
|
166
|
|
|
|
|
|
|
The id (I<str>) of the sequence. |
|
167
|
|
|
|
|
|
|
|
|
168
|
|
|
|
|
|
|
=item Returns |
|
169
|
|
|
|
|
|
|
|
|
170
|
|
|
|
|
|
|
The sequence (L<Bio::Seq>) or C<undef>. |
|
171
|
|
|
|
|
|
|
|
|
172
|
|
|
|
|
|
|
=back |
|
173
|
|
|
|
|
|
|
|
|
174
|
|
|
|
|
|
|
=cut |
|
175
|
|
|
|
|
|
|
|
|
176
|
|
|
|
|
|
|
sub search_sequence { |
|
177
|
2
|
|
|
2
|
1
|
782
|
my($self, $value) = @_; |
|
178
|
2
|
50
|
|
|
|
9
|
return unless defined $value; |
|
179
|
2
|
|
|
|
|
3
|
for my $seq (@{$self->get_sequences}){ |
|
|
2
|
|
|
|
|
8
|
|
|
180
|
4
|
|
|
|
|
43
|
(my $sid = $seq->display_id) =~ s/^(?:[^\s]*\|)?([^\s^\|]+?)\|?$/$1/; |
|
181
|
4
|
100
|
66
|
|
|
73
|
return $seq if $seq->display_id eq $value or $sid eq $value; |
|
182
|
|
|
|
|
|
|
} |
|
183
|
0
|
|
|
|
|
0
|
return; |
|
184
|
|
|
|
|
|
|
} |
|
185
|
|
|
|
|
|
|
|
|
186
|
|
|
|
|
|
|
=head2 name |
|
187
|
|
|
|
|
|
|
|
|
188
|
|
|
|
|
|
|
=over |
|
189
|
|
|
|
|
|
|
|
|
190
|
|
|
|
|
|
|
=item |
|
191
|
|
|
|
|
|
|
|
|
192
|
|
|
|
|
|
|
Gets/sets the name of the genome. If no name is set, tries to use the file instead. |
|
193
|
|
|
|
|
|
|
|
|
194
|
|
|
|
|
|
|
=back |
|
195
|
|
|
|
|
|
|
|
|
196
|
|
|
|
|
|
|
=cut |
|
197
|
|
|
|
|
|
|
|
|
198
|
|
|
|
|
|
|
sub name { |
|
199
|
7
|
|
|
7
|
1
|
13
|
my ($self, $value) = @_; |
|
200
|
7
|
50
|
|
|
|
43
|
$self->{'_name'} = $value if defined $value; |
|
201
|
7
|
100
|
|
|
|
26
|
unless(defined $self->{'_name'}){ |
|
202
|
6
|
|
|
|
|
48
|
my $f = $self->file; |
|
203
|
6
|
100
|
|
|
|
20
|
return unless defined $f; |
|
204
|
1
|
|
|
|
|
5
|
$f =~ s/.*\///; |
|
205
|
1
|
|
|
|
|
6
|
$f =~ s/\.[^\.]+$//; |
|
206
|
1
|
|
|
|
|
3
|
$self->{'_name'} = $f; |
|
207
|
|
|
|
|
|
|
} |
|
208
|
2
|
|
|
|
|
9
|
return $self->{'_name'}; |
|
209
|
|
|
|
|
|
|
} |
|
210
|
|
|
|
|
|
|
|
|
211
|
|
|
|
|
|
|
=head1 INTERNAL METHODS |
|
212
|
|
|
|
|
|
|
|
|
213
|
|
|
|
|
|
|
Methods intended to be used only within the scope of Bio::Polloc::* |
|
214
|
|
|
|
|
|
|
|
|
215
|
|
|
|
|
|
|
=head2 _initialize |
|
216
|
|
|
|
|
|
|
|
|
217
|
|
|
|
|
|
|
=cut |
|
218
|
|
|
|
|
|
|
|
|
219
|
|
|
|
|
|
|
sub _initialize { |
|
220
|
5
|
|
|
5
|
|
14
|
my ($self, @args) = @_; |
|
221
|
5
|
|
|
|
|
39
|
my($name, $file) = $self->_rearrange([qw(NAME FILE)], @args); |
|
222
|
5
|
|
|
|
|
21
|
$self->name($name); |
|
223
|
5
|
|
|
|
|
13
|
$self->file($file); |
|
224
|
|
|
|
|
|
|
} |
|
225
|
|
|
|
|
|
|
|
|
226
|
|
|
|
|
|
|
1; |