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sub |
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package Bio::Roary::External::CheckTools; |
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$Bio::Roary::External::CheckTools::VERSION = '3.10.1'; |
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# ABSTRACT: Check external executables are available and are the correct version |
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2
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2
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102970
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use Moose; |
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2
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382082
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2
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13
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7
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2
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2
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12089
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use File::Spec; |
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5
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2
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966
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2
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2
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664
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use Log::Log4perl qw(:easy); |
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31217
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2
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17
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has 'logger' => ( is => 'ro', lazy => 1, builder => '_build_logger' ); |
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sub _build_logger { |
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1
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1
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my ($self) = @_; |
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1
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8
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Log::Log4perl->easy_init($DEBUG); |
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14
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1
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2653
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my $logger = get_logger(); |
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1
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87
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return $logger; |
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} |
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my $BIDEC = '(\d+\.\d+)'; # pattern of NN.NN for versions that can be compared |
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my %tools = ( |
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'parallel' => { |
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GETVER => "parallel --version | grep '^GNU parallel 2'", |
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REGEXP => qr/GNU parallel (\d+)/, |
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MINVER => "20130422", |
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NEEDED => 1, |
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}, |
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'blastp' => { |
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GETVER => "blastp -version", |
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REGEXP => qr/blastp:\s+(\d+\.\d+\.\d+)/, |
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NEEDED => 1, |
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31
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}, |
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'makeblastdb' => { |
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GETVER => "makeblastdb -version", |
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REGEXP => qr/makeblastdb:\s+(\d+\.\d+\.\d+)/, |
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NEEDED => 1, |
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36
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}, |
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37
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'mcl' => { |
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38
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GETVER => "mcl --version | head -n 1", |
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REGEXP => qr/(\d+\-\d+)/, |
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40
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NEEDED => 1, |
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41
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}, |
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42
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'bedtools' => { |
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43
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GETVER => "bedtools --version", |
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44
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REGEXP => qr/bedtools v($BIDEC)/, |
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45
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MINVER => "2.1", |
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46
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NEEDED => 1, |
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47
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}, |
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48
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'mafft' => { |
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49
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GETVER => "mafft --version < /dev/null 2>&1", |
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50
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REGEXP => qr/(\d+\.\d+) /, |
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51
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NEEDED => 1, |
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52
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}, |
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53
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'kraken' => { |
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54
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GETVER => "kraken --version | head -n 1", |
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55
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REGEXP => qr/Kraken version kraken-(\d+\.\d+\.\d+.*)/, |
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56
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NEEDED => 0, |
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57
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}, |
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58
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'kraken-report' => { |
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59
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GETVER => "kraken-report --version | head -n 1", |
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60
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REGEXP => qr/Kraken version kraken-(\d+\.\d+\.\d+.*)/, |
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61
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NEEDED => 0, |
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62
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}, |
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63
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'Rscript' => { |
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64
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GETVER => "Rscript --version 2>&1 | head -n 1", |
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65
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REGEXP => qr/R scripting front-end version ($BIDEC)/, |
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66
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MINVER => "3", |
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67
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NEEDED => 0, |
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68
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}, |
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69
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70
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# prank version also performs an update check so cant use it |
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71
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'prank' => { NEEDED => 0 }, |
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72
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73
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# now just the standard unix tools we need |
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74
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'grep' => { NEEDED => 1 }, |
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75
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'sed' => { NEEDED => 1 }, |
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76
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'awk' => { NEEDED => 1 }, |
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77
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78
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); |
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79
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80
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my %cdhit_tools = ( |
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81
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'cdhit' => { |
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82
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GETVER => "cdhit -h | grep 'CD-HIT version'", |
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83
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REGEXP => qr/version ($BIDEC) /, |
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84
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MINVER => "4.6", |
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85
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}, |
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86
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'cd-hit' => { |
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87
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GETVER => "cd-hit -h | grep 'CD-HIT version'", |
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88
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REGEXP => qr/version ($BIDEC) /, |
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89
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MINVER => "4.6", |
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90
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} |
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91
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); |
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92
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93
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my %fasttree_tools = ( |
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94
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'fasttree' => { |
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95
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GETVER => "fasttree 2>&1 | head -n 1", |
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96
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REGEXP => qr/Usage for FastTree version ($BIDEC)/, |
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97
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}, |
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98
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'FastTree' => { |
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99
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GETVER => "FastTree 2>&1 | head -n 1", |
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100
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REGEXP => qr/Usage for FastTree version ($BIDEC)/, |
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101
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} |
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102
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); |
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103
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104
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sub which_tool_exec { |
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105
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2
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2
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0
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4
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my ( $self, $alt_tools ) = @_; |
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106
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2
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3
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for my $toolname ( sort keys %{$alt_tools} ) { |
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2
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8
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107
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4
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7
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my $fp = $self->find_exe($toolname); |
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108
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4
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50
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9
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return $toolname if $fp; |
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109
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} |
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110
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2
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54
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$self->logger->error( "Required tool missing. Can't find one of " . join( '/', keys %{$alt_tools} ) . " in your \$PATH." ); |
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2
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10
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111
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2
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516
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return undef; |
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112
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} |
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113
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114
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sub check_tool { |
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115
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23
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23
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0
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17682
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my ( $self, $toolname ) = @_; |
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116
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23
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43
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my $t = $tools{$toolname}; |
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117
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23
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37
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my $fp = $self->find_exe($toolname); |
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118
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23
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100
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100
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378
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$self->logger->error("ERROR: Can't find required '$toolname' in your \$PATH") if !$fp and $t->{NEEDED}; |
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119
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23
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100
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100
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3377
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$self->logger->error("Optional tool '$toolname' not found in your \$PATH") if !$fp and !$t->{NEEDED}; |
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120
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121
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23
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100
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1263
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if ($fp) { |
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122
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6
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22
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$t->{HAVE} = $fp; |
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123
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6
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157
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$self->logger->warn("Looking for '$toolname' - found $fp"); |
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124
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6
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50
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1534
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if ( $t->{GETVER} ) { |
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125
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0
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0
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my ($s) = qx($t->{GETVER}); |
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126
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0
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0
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0
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if ( defined $s ) { |
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127
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0
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0
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$s =~ $t->{REGEXP}; |
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128
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0
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0
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0
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$t->{VERSION} = $1 if defined $1; |
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129
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0
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0
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$self->logger->warn("Determined $toolname version is $t->{VERSION}"); |
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130
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0
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0
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0
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0
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if ( defined $t->{MINVER} and $t->{VERSION} < $t->{MINVER} ) { |
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131
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0
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0
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$self->logger->error("Roary needs $toolname $t->{MINVER} or higher. Please upgrade and try again."); |
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132
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} |
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133
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0
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0
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0
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0
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if ( defined $t->{MAXVER} and $t->{VERSION} > $t->{MAXVER} ) { |
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134
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0
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0
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$self->logger->error( |
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135
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"Roary needs a version of $toolname between $t->{MINVER} and $t->{MAXVER}. Please downgrade and try again."); |
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136
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} |
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137
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} |
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138
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else { |
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139
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0
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0
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$self->logger->error( "Could not determine version of $toolname - please install version ", $t->{MINVER}, " or higher" ) |
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140
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; # FIXME: or less <= MAXVER if given |
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141
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} |
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142
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} |
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143
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} |
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144
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} |
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145
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146
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sub check_all_tools { |
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147
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1
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1
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0
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1035
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my ($self) = @_; |
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148
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1
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6
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$ENV{"GREP_OPTIONS"} = ''; # --colour => version grep fails (Issue #117) |
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149
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1
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10
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for my $toolname ( sort keys %tools ) { |
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150
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13
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26
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$self->check_tool($toolname); |
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151
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} |
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152
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153
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1
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5
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my $cdhit = $self->which_tool_exec( \%cdhit_tools ); |
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154
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1
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50
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4
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if ($cdhit) { |
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155
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0
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0
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$tools{$cdhit} = $cdhit_tools{$cdhit}; |
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156
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0
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0
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$self->check_tool($cdhit); |
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157
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} |
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158
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159
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1
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4
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my $fasttree = $self->which_tool_exec( \%fasttree_tools ); |
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160
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1
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50
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4
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if ($fasttree) { |
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161
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0
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0
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$tools{$fasttree} = $fasttree_tools{$fasttree}; |
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162
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0
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0
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$self->check_tool($fasttree); |
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163
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} |
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164
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165
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1
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5
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return $self; |
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166
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} |
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167
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168
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sub find_exe { |
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169
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27
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27
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0
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32
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my ( $self, $bin ) = @_; |
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170
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27
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229
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for my $dir ( File::Spec->path ) { |
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171
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233
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1059
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my $exe = File::Spec->catfile( $dir, $bin ); |
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172
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233
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100
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1138
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return $exe if -x $exe; |
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173
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} |
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174
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21
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52
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return; |
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175
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} |
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176
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177
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2
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2
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|
3768
|
no Moose; |
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2
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6
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2
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16
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178
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__PACKAGE__->meta->make_immutable; |
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179
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180
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1; |
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181
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182
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__END__ |
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183
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184
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=pod |
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185
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186
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|
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=encoding UTF-8 |
|
187
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|
188
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=head1 NAME |
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189
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|
190
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|
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Bio::Roary::External::CheckTools - Check external executables are available and are the correct version |
|
191
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|
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|
192
|
|
|
|
|
|
|
=head1 VERSION |
|
193
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194
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version 3.10.1 |
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195
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196
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=head1 SYNOPSIS |
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197
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Functionality borrowed from PROKKA by Torsten Seemann. |
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198
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Check external executables are available and are the correct version |
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199
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200
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use Bio::Roary::External::CheckTools; |
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201
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202
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my $obj = Bio::Roary::External::CheckTools->new(); |
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203
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$obj->check_all_tools; |
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204
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205
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=head1 AUTHOR |
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206
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207
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Andrew J. Page <ap13@sanger.ac.uk> |
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208
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209
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=head1 COPYRIGHT AND LICENSE |
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210
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211
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This software is Copyright (c) 2013 by Wellcome Trust Sanger Institute. |
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212
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213
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This is free software, licensed under: |
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214
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215
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The GNU General Public License, Version 3, June 2007 |
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217
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=cut |