|  line  | 
 stmt  | 
 bran  | 
 cond  | 
 sub  | 
 pod  | 
 time  | 
 code  | 
| 
1
 | 
  
 
  
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 package Bio::Phylo::Forest::Node;  | 
| 
2
 | 
33
 | 
 
 | 
 
 | 
  
33
  
 | 
 
 | 
62618
 | 
 use strict;  | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
72
 | 
    | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
793
 | 
    | 
| 
3
 | 
33
 | 
 
 | 
 
 | 
  
33
  
 | 
 
 | 
146
 | 
 use warnings;  | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
59
 | 
    | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
810
 | 
    | 
| 
4
 | 
33
 | 
 
 | 
 
 | 
  
33
  
 | 
 
 | 
9550
 | 
 use Bio::Phylo::Forest::DrawNodeRole;  | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
88
 | 
    | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
306
 | 
    | 
| 
5
 | 
33
 | 
 
 | 
 
 | 
  
33
  
 | 
 
 | 
193
 | 
 use base qw'Bio::Phylo::Forest::DrawNodeRole';  | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
94
 | 
    | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
2857
 | 
    | 
| 
6
 | 
33
 | 
 
 | 
 
 | 
  
33
  
 | 
 
 | 
207
 | 
 use Bio::Phylo::Util::CONSTANT qw':objecttypes /looks_like/';  | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
61
 | 
    | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
7853
 | 
    | 
| 
7
 | 
33
 | 
 
 | 
 
 | 
  
33
  
 | 
 
 | 
221
 | 
 use Bio::Phylo::Util::Exceptions 'throw';  | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
63
 | 
    | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
1454
 | 
    | 
| 
8
 | 
33
 | 
 
 | 
 
 | 
  
33
  
 | 
 
 | 
181
 | 
 use Scalar::Util 'weaken';  | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
63
 | 
    | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
8851
 | 
    | 
| 
9
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
10
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 # store type constant  | 
| 
11
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 my ( $TYPE_CONSTANT, $CONTAINER_CONSTANT ) = ( _NODE_, _TREE_ );  | 
| 
12
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
13
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 {      | 
| 
14
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
15
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     # @fields array necessary for object destruction  | 
| 
16
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     my @fields = \( my ( %branch_length, %parent, %tree, %rank ) );  | 
| 
17
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
18
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =head1 NAME  | 
| 
19
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
20
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 Bio::Phylo::Forest::Node - Node in a phylogenetic tree  | 
| 
21
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
22
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =head1 SYNOPSIS  | 
| 
23
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
24
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  # some way to get nodes:  | 
| 
25
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  use Bio::Phylo::IO;  | 
| 
26
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  my $string = '((A,B),C);';  | 
| 
27
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  my $forest = Bio::Phylo::IO->parse(  | 
| 
28
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     -format => 'newick',  | 
| 
29
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     -string => $string  | 
| 
30
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  );  | 
| 
31
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
32
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  # prints 'Bio::Phylo::Forest'  | 
| 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  print ref $forest;  | 
| 
34
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
35
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  foreach my $tree ( @{ $forest->get_entities } ) {  | 
| 
36
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
37
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     # prints 'Bio::Phylo::Forest::Tree'  | 
| 
38
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     print ref $tree;  | 
| 
39
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
40
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     foreach my $node ( @{ $tree->get_entities } ) {  | 
| 
41
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
42
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
        # prints 'Bio::Phylo::Forest::Node'  | 
| 
43
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
        print ref $node;  | 
| 
44
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
45
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
        # node has a parent, i.e. is not root  | 
| 
46
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
        if ( $node->get_parent ) {  | 
| 
47
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
           $node->set_branch_length(1);  | 
| 
48
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
        }  | 
| 
49
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
50
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
        # node is root  | 
| 
51
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
        else {  | 
| 
52
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
           $node->set_branch_length(0);  | 
| 
53
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
        }  | 
| 
54
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     }  | 
| 
55
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  }  | 
| 
56
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
57
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =head1 DESCRIPTION  | 
| 
58
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
59
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 This module has the getters and setters that alter the state of a   | 
| 
60
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 node object. Useful behaviours (which are also available) are defined  | 
| 
61
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 in the L<Bio::Phylo::Forest::NodeRole> package.  | 
| 
62
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
63
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =head1 METHODS  | 
| 
64
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
65
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =cut  | 
| 
66
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
67
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     my $set_raw_parent = sub {  | 
| 
68
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         my ( $self, $parent ) = @_;  | 
| 
69
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         my $id = $self->get_id;  | 
| 
70
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         $parent{$id} = $parent;    # XXX here we modify parent  | 
| 
71
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         weaken $parent{$id} if $parent;  | 
| 
72
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     };  | 
| 
73
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     my $get_parent = sub {  | 
| 
74
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         my $self = shift;  | 
| 
75
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         return $parent{ $self->get_id };  | 
| 
76
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     };  | 
| 
77
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     my $get_children = sub { shift->get_entities };  | 
| 
78
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     my $get_branch_length = sub {  | 
| 
79
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         my $self = shift;  | 
| 
80
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         return $branch_length{ $self->get_id };  | 
| 
81
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     };  | 
| 
82
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     my $set_raw_child = sub {  | 
| 
83
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         my ( $self, $child, $i ) = @_;  | 
| 
84
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         $i = $self->last_index + 1 if not defined $i or $i == -1;  | 
| 
85
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         $self->insert_at_index( $child, $i );    # XXX here we modify children  | 
| 
86
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     };      | 
| 
87
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
88
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =over  | 
| 
89
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
90
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =item set_parent()  | 
| 
91
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
92
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 Sets argument as invocant's parent.  | 
| 
93
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
94
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Type    : Mutator  | 
| 
95
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Title   : set_parent  | 
| 
96
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Usage   : $node->set_parent($parent);  | 
| 
97
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Function: Assigns a node's parent.  | 
| 
98
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Returns : Modified object.  | 
| 
99
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Args    : If no argument is given, the current  | 
| 
100
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
            parent is set to undefined. A valid  | 
| 
101
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
            argument is Bio::Phylo::Forest::Node  | 
| 
102
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
            object.  | 
| 
103
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
104
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =cut  | 
| 
105
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
106
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     sub set_parent : Clonable {  | 
| 
107
 | 
810
 | 
 
 | 
 
 | 
  
810
  
 | 
  
1
  
 | 
1347
 | 
         my ( $self, $parent ) = @_;  | 
| 
108
 | 
810
 | 
  
100
  
 | 
  
100
  
 | 
 
 | 
 
 | 
2336
 | 
         if ( $parent and looks_like_object $parent, $TYPE_CONSTANT ) {  | 
| 
 
 | 
 
 | 
  
 50
  
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
109
 | 
675
 | 
 
 | 
 
 | 
 
 | 
 
 | 
1412
 | 
             $parent->set_child($self);  | 
| 
110
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         }  | 
| 
111
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         elsif ( not $parent ) {  | 
| 
112
 | 
134
 | 
 
 | 
 
 | 
 
 | 
 
 | 
341
 | 
             $self->set_raw_parent;  | 
| 
113
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         }  | 
| 
114
 | 
809
 | 
 
 | 
 
 | 
 
 | 
 
 | 
1602
 | 
         return $self;  | 
| 
115
 | 
33
 | 
 
 | 
 
 | 
  
33
  
 | 
 
 | 
215
 | 
     }  | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
66
 | 
    | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
181
 | 
    | 
| 
116
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
117
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =item set_raw_parent()  | 
| 
118
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
119
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 Sets argument as invocant's parent. This method does NO   | 
| 
120
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 sanity checks on the rest of the topology. Use with caution.  | 
| 
121
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
122
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Type    : Mutator  | 
| 
123
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Title   : set_raw_parent  | 
| 
124
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Usage   : $node->set_raw_parent($parent);  | 
| 
125
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Function: Assigns a node's parent.  | 
| 
126
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Returns : Modified object.  | 
| 
127
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Args    : If no argument is given, the current  | 
| 
128
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
            parent is set to undefined. A valid  | 
| 
129
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
            argument is Bio::Phylo::Forest::Node  | 
| 
130
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
            object.  | 
| 
131
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
132
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =cut  | 
| 
133
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
134
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 	sub set_raw_parent {  | 
| 
135
 | 
9779
 | 
 
 | 
 
 | 
  
9779
  
 | 
  
1
  
 | 
18448
 | 
 		$set_raw_parent->(@_)  | 
| 
136
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 	}  | 
| 
137
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
138
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =item set_child()  | 
| 
139
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
140
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 Sets argument as invocant's child.  | 
| 
141
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
142
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Type    : Mutator  | 
| 
143
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Title   : set_child  | 
| 
144
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Usage   : $node->set_child($child);  | 
| 
145
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Function: Assigns a new child to $node  | 
| 
146
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Returns : Modified object.  | 
| 
147
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Args    : A valid argument consists of a  | 
| 
148
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
            Bio::Phylo::Forest::Node object.  | 
| 
149
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
150
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =cut  | 
| 
151
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
152
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     sub set_child {  | 
| 
153
 | 
10379
 | 
 
 | 
 
 | 
  
10379
  
 | 
  
1
  
 | 
18580
 | 
         my ( $self, $child, $i ) = @_;  | 
| 
154
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
155
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         # bad args?  | 
| 
156
 | 
10379
 | 
  
100
  
 | 
  
 66
  
 | 
 
 | 
 
 | 
28762
 | 
         if ( not $child or not looks_like_object $child, $TYPE_CONSTANT ) {  | 
| 
157
 | 
460
 | 
 
 | 
 
 | 
 
 | 
 
 | 
659
 | 
             return;  | 
| 
158
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         }  | 
| 
159
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
160
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         # maybe nothing to do?  | 
| 
161
 | 
9917
 | 
  
100
  
 | 
  
 33
  
 | 
 
 | 
 
 | 
28357
 | 
         if (   not $child  | 
| 
 
 | 
 
 | 
 
 | 
  
 66
  
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
162
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
             or $child->get_id == $self->get_id  | 
| 
163
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
             or $child->is_child_of($self) )  | 
| 
164
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         {  | 
| 
165
 | 
312
 | 
 
 | 
 
 | 
 
 | 
 
 | 
539
 | 
             return $self;  | 
| 
166
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         }  | 
| 
167
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
168
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         # $child_parent is NEVER $self, see above  | 
| 
169
 | 
9605
 | 
 
 | 
 
 | 
 
 | 
 
 | 
17699
 | 
         my $child_parent = $child->get_parent;  | 
| 
170
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
171
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         # child is ancestor: this is obviously problematic, because  | 
| 
172
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         # now we're trying to set a node nearer to the root on the  | 
| 
173
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         # same lineage as the CHILD of a descendant. Because they're  | 
| 
174
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         # on the same lineage it's hard to see how this can be done  | 
| 
175
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         # sensibly. The decision here is to do:  | 
| 
176
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         # 	1. we prune what is to become the parent (now the descendant)  | 
| 
177
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         #	   from its current parent  | 
| 
178
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         #	2. we set this pruned node (and its descendants) as a sibling  | 
| 
179
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         #	   of what is to become the child  | 
| 
180
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         #	3. we prune what is to become the child from its parent  | 
| 
181
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         #	4. we set that pruned child as the child of $self  | 
| 
182
 | 
9605
 | 
  
100
  
 | 
 
 | 
 
 | 
 
 | 
20587
 | 
         if ( $child->is_ancestor_of($self) ) {  | 
| 
183
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
184
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
             # step 1.  | 
| 
185
 | 
1
 | 
 
 | 
 
 | 
 
 | 
 
 | 
3
 | 
             my $parent_parent = $self->get_parent;  | 
| 
186
 | 
1
 | 
 
 | 
 
 | 
 
 | 
 
 | 
12
 | 
             $parent_parent->prune_child($self);  | 
| 
187
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
188
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
             # step 2.  | 
| 
189
 | 
1
 | 
 
 | 
 
 | 
 
 | 
 
 | 
3
 | 
             $self->set_raw_parent( $child_parent );    # XXX could be undef  | 
| 
190
 | 
1
 | 
  
 50
  
 | 
 
 | 
 
 | 
 
 | 
4
 | 
             if ($child_parent) {  | 
| 
191
 | 
  
0
  
 | 
 
 | 
 
 | 
 
 | 
 
 | 
0
 | 
                 $child_parent->set_raw_child( $self );  | 
| 
192
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
             }  | 
| 
193
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         }  | 
| 
194
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
195
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         # step 3.  | 
| 
196
 | 
9605
 | 
  
100
  
 | 
 
 | 
 
 | 
 
 | 
18048
 | 
         if ($child_parent) {  | 
| 
197
 | 
235
 | 
 
 | 
 
 | 
 
 | 
 
 | 
550
 | 
             $child_parent->prune_child($child);  | 
| 
198
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         }  | 
| 
199
 | 
9605
 | 
 
 | 
 
 | 
 
 | 
 
 | 
23428
 | 
         $child->set_raw_parent( $self );  | 
| 
200
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
201
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         # now do the insert, first make room by shifting later siblings right  | 
| 
202
 | 
9605
 | 
 
 | 
 
 | 
 
 | 
 
 | 
18152
 | 
         my $children = $self->get_children;  | 
| 
203
 | 
9605
 | 
  
 50
  
 | 
 
 | 
 
 | 
 
 | 
20084
 | 
         if ( defined $i ) {  | 
| 
204
 | 
  
0
  
 | 
 
 | 
 
 | 
 
 | 
 
 | 
0
 | 
             for ( my $j = $#{$children} ; $j >= 0 ; $j-- ) {  | 
| 
 
 | 
  
0
  
 | 
 
 | 
 
 | 
 
 | 
 
 | 
0
 | 
    | 
| 
205
 | 
  
0
  
 | 
 
 | 
 
 | 
 
 | 
 
 | 
0
 | 
                 my $sibling = $children->[$j];  | 
| 
206
 | 
  
0
  
 | 
 
 | 
 
 | 
 
 | 
 
 | 
0
 | 
                 $self->set_raw_child( $sibling, $j + 1 );  | 
| 
207
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
             }  | 
| 
208
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         }  | 
| 
209
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
210
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         # no index was supplied, child becomes last daughter  | 
| 
211
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         else {  | 
| 
212
 | 
9605
 | 
 
 | 
 
 | 
 
 | 
 
 | 
12467
 | 
             $i = scalar @{$children};  | 
| 
 
 | 
9605
 | 
 
 | 
 
 | 
 
 | 
 
 | 
13557
 | 
    | 
| 
213
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         }  | 
| 
214
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
215
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         # step 4.  | 
| 
216
 | 
9605
 | 
 
 | 
 
 | 
 
 | 
 
 | 
24808
 | 
         $self->set_raw_child( $child, $i );  | 
| 
217
 | 
9605
 | 
 
 | 
 
 | 
 
 | 
 
 | 
19921
 | 
         return $self;  | 
| 
218
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     }  | 
| 
219
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
220
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =item set_raw_child()  | 
| 
221
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
222
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 Sets argument as invocant's child. This method does NO   | 
| 
223
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 sanity checks on the rest of the topology. Use with caution.  | 
| 
224
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
225
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Type    : Mutator  | 
| 
226
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Title   : set_raw_child  | 
| 
227
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Usage   : $node->set_raw_child($child);  | 
| 
228
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Function: Assigns a new child to $node  | 
| 
229
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Returns : Modified object.  | 
| 
230
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Args    : A valid argument consists of a  | 
| 
231
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
            Bio::Phylo::Forest::Node object.  | 
| 
232
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
233
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =cut  | 
| 
234
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
235
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 	sub set_raw_child {  | 
| 
236
 | 
9644
 | 
 
 | 
 
 | 
  
9644
  
 | 
  
1
  
 | 
19034
 | 
 		$set_raw_child->(@_);  | 
| 
237
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 	}  | 
| 
238
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
       | 
| 
239
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =item set_branch_length()  | 
| 
240
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
241
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 Sets argument as invocant's branch length.  | 
| 
242
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
243
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Type    : Mutator  | 
| 
244
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Title   : set_branch_length  | 
| 
245
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Usage   : $node->set_branch_length(0.423e+2);  | 
| 
246
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Function: Assigns a node's branch length.  | 
| 
247
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Returns : Modified object.  | 
| 
248
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Args    : If no argument is given, the  | 
| 
249
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
            current branch length is set  | 
| 
250
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
            to undefined. A valid argument  | 
| 
251
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
            is a number in any of Perl's formats.  | 
| 
252
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
253
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =cut  | 
| 
254
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
255
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     sub set_branch_length : Clonable {  | 
| 
256
 | 
61051
 | 
 
 | 
 
 | 
  
61051
  
 | 
  
1
  
 | 
95851
 | 
         my ( $self, $bl ) = @_;  | 
| 
257
 | 
61051
 | 
 
 | 
 
 | 
 
 | 
 
 | 
98200
 | 
         my $id = $self->get_id;  | 
| 
258
 | 
61051
 | 
  
100
  
 | 
  
100
  
 | 
 
 | 
 
 | 
146559
 | 
         if ( defined $bl && looks_like_number $bl && !ref $bl ) {  | 
| 
 
 | 
 
 | 
  
100
  
 | 
  
 66
  
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
 
 | 
 
 | 
  
 50
  
 | 
  
 33
  
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
 
 | 
 
 | 
 
 | 
  
 66
  
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
259
 | 
61040
 | 
 
 | 
 
 | 
 
 | 
 
 | 
93513
 | 
             $branch_length{$id} = $bl;  | 
| 
260
 | 
61040
 | 
  
 50
  
 | 
 
 | 
 
 | 
 
 | 
105424
 | 
 			if ( $bl < 0 ) {  | 
| 
261
 | 
  
0
  
 | 
 
 | 
 
 | 
 
 | 
 
 | 
0
 | 
 				$self->get_logger->warn("Setting length < 0: $bl");  | 
| 
262
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 			}  | 
| 
263
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         }  | 
| 
264
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         elsif ( defined $bl && ( !looks_like_number $bl || ref $bl ) ) {  | 
| 
265
 | 
1
 | 
 
 | 
 
 | 
 
 | 
 
 | 
6
 | 
             throw 'BadNumber' => "Branch length \"$bl\" is a bad number";  | 
| 
266
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         }  | 
| 
267
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         elsif ( !defined $bl ) {  | 
| 
268
 | 
10
 | 
 
 | 
 
 | 
 
 | 
 
 | 
22
 | 
             $branch_length{$id} = undef;  | 
| 
269
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         }  | 
| 
270
 | 
61050
 | 
 
 | 
 
 | 
 
 | 
 
 | 
136320
 | 
         return $self;  | 
| 
271
 | 
33
 | 
 
 | 
 
 | 
  
33
  
 | 
 
 | 
18124
 | 
     }  | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
78
 | 
    | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
117
 | 
    | 
| 
272
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
273
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =item set_tree()  | 
| 
274
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
275
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 Sets what tree invocant belongs to  | 
| 
276
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
277
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Type    : Mutator  | 
| 
278
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Title   : set_tree  | 
| 
279
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Usage   : $node->set_tree($tree);  | 
| 
280
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Function: Sets what tree invocant belongs to  | 
| 
281
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Returns : Invocant  | 
| 
282
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Args    : Bio::Phylo::Forest::Tree  | 
| 
283
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Comments: This method is called automatically   | 
| 
284
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
            when inserting or deleting nodes in  | 
| 
285
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
            trees.  | 
| 
286
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
287
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =cut  | 
| 
288
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
289
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     sub set_tree : Clonable {  | 
| 
290
 | 
9883
 | 
 
 | 
 
 | 
  
9883
  
 | 
  
1
  
 | 
15705
 | 
         my ( $self, $tree ) = @_;  | 
| 
291
 | 
9883
 | 
 
 | 
 
 | 
 
 | 
 
 | 
17360
 | 
         my $id = $self->get_id;  | 
| 
292
 | 
9883
 | 
  
100
  
 | 
 
 | 
 
 | 
 
 | 
18344
 | 
         if ($tree) {  | 
| 
293
 | 
9608
 | 
  
 50
  
 | 
 
 | 
 
 | 
 
 | 
21539
 | 
             if ( looks_like_object $tree, $CONTAINER_CONSTANT ) {  | 
| 
294
 | 
9608
 | 
 
 | 
 
 | 
 
 | 
 
 | 
20410
 | 
                 $tree{$id} = $tree;  | 
| 
295
 | 
9608
 | 
 
 | 
 
 | 
 
 | 
 
 | 
25464
 | 
                 weaken $tree{$id};  | 
| 
296
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
             }  | 
| 
297
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
             else {  | 
| 
298
 | 
  
0
  
 | 
 
 | 
 
 | 
 
 | 
 
 | 
0
 | 
                 throw 'ObjectMismatch' => "$tree is not a tree";  | 
| 
299
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
             }  | 
| 
300
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         }  | 
| 
301
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         else {  | 
| 
302
 | 
275
 | 
 
 | 
 
 | 
 
 | 
 
 | 
768
 | 
             $tree{$id} = undef;  | 
| 
303
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         }  | 
| 
304
 | 
9883
 | 
 
 | 
 
 | 
 
 | 
 
 | 
28696
 | 
         return $self;  | 
| 
305
 | 
33
 | 
 
 | 
 
 | 
  
33
  
 | 
 
 | 
8994
 | 
     }  | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
74
 | 
    | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
156
 | 
    | 
| 
306
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
307
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =item set_rank()  | 
| 
308
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
309
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 Sets the taxonomic rank of the node  | 
| 
310
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
311
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Type    : Mutator  | 
| 
312
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Title   : set_rank  | 
| 
313
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Usage   : $node->set_rank('genus');  | 
| 
314
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Function: Sets the taxonomic rank of the node  | 
| 
315
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Returns : Invocant  | 
| 
316
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Args    : String  | 
| 
317
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Comments: Free-form, but highly recommended to use same rank names as in Bio::Taxon  | 
| 
318
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
319
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =cut  | 
| 
320
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
321
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
       | 
| 
322
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     sub set_rank : Clonable {  | 
| 
323
 | 
2
 | 
 
 | 
 
 | 
  
2
  
 | 
  
1
  
 | 
4
 | 
     	my ( $self, $rank ) = @_;  | 
| 
324
 | 
2
 | 
 
 | 
 
 | 
 
 | 
 
 | 
5
 | 
     	$rank{$self->get_id} = $rank;  | 
| 
325
 | 
2
 | 
 
 | 
 
 | 
 
 | 
 
 | 
9
 | 
     	return $self;  | 
| 
326
 | 
33
 | 
 
 | 
 
 | 
  
33
  
 | 
 
 | 
7361
 | 
     }  | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
95
 | 
    | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
144
 | 
    | 
| 
327
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
328
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =item get_parent()  | 
| 
329
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
330
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 Gets invocant's parent.  | 
| 
331
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
332
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Type    : Accessor  | 
| 
333
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Title   : get_parent  | 
| 
334
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Usage   : my $parent = $node->get_parent;  | 
| 
335
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Function: Retrieves a node's parent.  | 
| 
336
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Returns : Bio::Phylo::Forest::Node  | 
| 
337
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Args    : NONE  | 
| 
338
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
339
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =cut  | 
| 
340
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
341
 | 
543139
 | 
 
 | 
 
 | 
  
543139
  
 | 
  
1
  
 | 
686741
 | 
     sub get_parent { return $get_parent->(shift) }      | 
| 
342
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
343
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =item get_branch_length()  | 
| 
344
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
345
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 Gets invocant's branch length.  | 
| 
346
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
347
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Type    : Accessor  | 
| 
348
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Title   : get_branch_length  | 
| 
349
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Usage   : my $branch_length = $node->get_branch_length;  | 
| 
350
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Function: Retrieves a node's branch length.  | 
| 
351
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Returns : FLOAT  | 
| 
352
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Args    : NONE  | 
| 
353
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Comments: Test for "defined($node->get_branch_length)"  | 
| 
354
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
            for zero-length (but defined) branches. Testing  | 
| 
355
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
            "if ( $node->get_branch_length ) { ... }"  | 
| 
356
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
            yields false for zero-but-defined branches!  | 
| 
357
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
358
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =cut  | 
| 
359
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
360
 | 
65975
 | 
 
 | 
 
 | 
  
65975
  
 | 
  
1
  
 | 
94964
 | 
     sub get_branch_length { return $get_branch_length->(shift) }  | 
| 
361
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
362
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =item get_children()  | 
| 
363
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
364
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 Gets invocant's immediate children.  | 
| 
365
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
366
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Type    : Query  | 
| 
367
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Title   : get_children  | 
| 
368
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Usage   : my @children = @{ $node->get_children };  | 
| 
369
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Function: Returns an array reference of immediate  | 
| 
370
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
            descendants, ordered from left to right.  | 
| 
371
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Returns : Array reference of  | 
| 
372
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
            Bio::Phylo::Forest::Node objects.  | 
| 
373
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Args    : NONE  | 
| 
374
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
375
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =cut  | 
| 
376
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
377
 | 
128969
 | 
 
 | 
 
 | 
  
128969
  
 | 
  
1
  
 | 
190158
 | 
     sub get_children { return $get_children->(shift) }  | 
| 
378
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
       | 
| 
379
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =item get_tree()  | 
| 
380
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
381
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 Returns the tree invocant belongs to  | 
| 
382
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
383
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Type    : Query  | 
| 
384
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Title   : get_tree  | 
| 
385
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Usage   : my $tree = $node->get_tree;  | 
| 
386
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Function: Returns the tree $node belongs to  | 
| 
387
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Returns : Bio::Phylo::Forest::Tree  | 
| 
388
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Args    : NONE  | 
| 
389
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
390
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =cut  | 
| 
391
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
392
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     sub get_tree {  | 
| 
393
 | 
123
 | 
 
 | 
 
 | 
  
123
  
 | 
  
1
  
 | 
175
 | 
         my $self = shift;  | 
| 
394
 | 
123
 | 
 
 | 
 
 | 
 
 | 
 
 | 
232
 | 
         my $id   = $self->get_id;  | 
| 
395
 | 
123
 | 
 
 | 
 
 | 
 
 | 
 
 | 
462
 | 
         return $tree{$id};  | 
| 
396
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     }  | 
| 
397
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
398
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =item get_rank()  | 
| 
399
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
400
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 Gets the taxonomic rank of the node  | 
| 
401
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
402
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Type    : Mutator  | 
| 
403
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Title   : get_rank  | 
| 
404
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Usage   : my $rank = $node->get_rank;  | 
| 
405
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Function: Gets the taxonomic rank of the node  | 
| 
406
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Returns : String  | 
| 
407
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Args    : NONE  | 
| 
408
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Comments:   | 
| 
409
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
410
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =cut  | 
| 
411
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
       | 
| 
412
 | 
2
 | 
 
 | 
 
 | 
  
2
  
 | 
  
1
  
 | 
6
 | 
     sub get_rank { $rank{shift->get_id} }  | 
| 
413
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
414
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =begin comment  | 
| 
415
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
416
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Type    : Internal method  | 
| 
417
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Title   : _json_data  | 
| 
418
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Usage   : $node->_json_data;  | 
| 
419
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Function: Populates a data structure to be serialized as JSON  | 
| 
420
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Returns :   | 
| 
421
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Args    :  | 
| 
422
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
423
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =end comment  | 
| 
424
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
425
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =cut  | 
| 
426
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
       | 
| 
427
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     sub _json_data {  | 
| 
428
 | 
  
0
  
 | 
 
 | 
 
 | 
  
0
  
 | 
 
 | 
 
 | 
     	my $self = shift;  | 
| 
429
 | 
  
0
  
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     	my %result = %{ $self->SUPER::_json_data };  | 
| 
 
 | 
  
0
  
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
430
 | 
  
0
  
 | 
  
  0
  
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     	$result{'length'}   = $self->get_branch_length if defined $self->get_branch_length;  | 
| 
431
 | 
  
0
  
 | 
  
  0
  
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     	$result{'rank'}     = $self->get_rank if $self->get_rank;  | 
| 
432
 | 
  
0
  
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     	$result{'children'} = [ map { $_->_json_data } @{ $self->get_children } ];  | 
| 
 
 | 
  
0
  
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
 
 | 
  
0
  
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
433
 | 
0
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     	return \%result;  | 
| 
434
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     }  | 
| 
435
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
436
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =begin comment  | 
| 
437
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
438
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Type    : Internal method  | 
| 
439
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Title   : _cleanup  | 
| 
440
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Usage   : $trees->_cleanup;  | 
| 
441
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Function: Called during object destruction, for cleanup of instance data  | 
| 
442
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Returns :   | 
| 
443
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
  Args    :  | 
| 
444
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
445
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =end comment  | 
| 
446
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
447
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =cut  | 
| 
448
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
449
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     sub _cleanup : Destructor {  | 
| 
450
 | 
9574
 | 
 
 | 
 
 | 
  
9574
  
 | 
 
 | 
11744
 | 
         my $self = shift;  | 
| 
451
 | 
9574
 | 
 
 | 
 
 | 
 
 | 
 
 | 
15914
 | 
         my $id   = $self->get_id;  | 
| 
452
 | 
9574
 | 
 
 | 
 
 | 
 
 | 
 
 | 
14592
 | 
         for my $field (@fields) {  | 
| 
453
 | 
38296
 | 
 
 | 
 
 | 
 
 | 
 
 | 
71574
 | 
             delete $field->{$id};  | 
| 
454
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
         }  | 
| 
455
 | 
33
 | 
 
 | 
 
 | 
  
33
  
 | 
 
 | 
12862
 | 
     }  | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
71
 | 
    | 
| 
 
 | 
33
 | 
 
 | 
 
 | 
 
 | 
 
 | 
120
 | 
    | 
| 
456
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
       | 
| 
457
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 }  | 
| 
458
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
459
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =back  | 
| 
460
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
461
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
     # podinherit_insert_token  | 
| 
462
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
463
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =head1 SEE ALSO  | 
| 
464
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
465
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 There is a mailing list at L<https://groups.google.com/forum/#!forum/bio-phylo>   | 
| 
466
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 for any user or developer questions and discussions.  | 
| 
467
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
468
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =over  | 
| 
469
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
470
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =item L<Bio::Phylo::Forest::NodeRole>  | 
| 
471
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
472
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 This object inherits from L<Bio::Phylo::Forest::NodeRole>, so methods  | 
| 
473
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 defined there are also applicable here.  | 
| 
474
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
475
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =item L<Bio::Phylo::Manual>  | 
| 
476
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
477
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 Also see the manual: L<Bio::Phylo::Manual> and L<http://rutgervos.blogspot.com>.  | 
| 
478
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
479
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =back  | 
| 
480
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
481
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =head1 CITATION  | 
| 
482
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
483
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 If you use Bio::Phylo in published research, please cite it:  | 
| 
484
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
485
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 B<Rutger A Vos>, B<Jason Caravas>, B<Klaas Hartmann>, B<Mark A Jensen>  | 
| 
486
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 and B<Chase Miller>, 2011. Bio::Phylo - phyloinformatic analysis using Perl.  | 
| 
487
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 I<BMC Bioinformatics> B<12>:63.  | 
| 
488
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 L<http://dx.doi.org/10.1186/1471-2105-12-63>  | 
| 
489
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
490
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 =cut  | 
| 
491
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
    | 
| 
492
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 
 | 
 1;  |