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package Bio::MUST::Apps::TwoScalp::Seqs2Profile; |
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# ABSTRACT: internal class for two-scalp tool |
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# CONTRIBUTOR: Amandine BERTRAND <amandine.bertrand@doct.uliege.be> |
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$Bio::MUST::Apps::TwoScalp::Seqs2Profile::VERSION = '0.201810'; |
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use Moose; |
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use namespace::autoclean; |
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use autodie; |
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use feature qw(say); |
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use Smart::Comments '###'; |
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use List::AllUtils qw(part); |
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use Bio::MUST::Core; |
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use Bio::MUST::Core::Constants qw(:gaps); |
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use Bio::MUST::Core::Utils qw(secure_outfile); |
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use aliased 'Bio::MUST::Core::Ali'; |
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use aliased 'Bio::MUST::Core::Seq'; |
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use aliased 'Bio::MUST::Core::SeqId'; |
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use aliased 'Bio::MUST::Core::SeqMask'; |
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use aliased 'Bio::MUST::Drivers::Blast::Database::Temporary'; |
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#use aliased 'Bio::MUST::Drivers::Blast::Query'; |
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use aliased 'Bio::MUST::Drivers::Mafft'; |
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use aliased 'Bio::MUST::Drivers::ClustalO'; |
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use aliased 'Bio::MUST::Apps::SlaveAligner::Local'; |
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use aliased 'Bio::MUST::Core::IdList'; |
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has 'file1' => ( |
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is => 'ro', |
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isa => 'Bio::MUST::Core::Ali', |
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required => 1, |
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coerce => 1, |
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); |
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has 'file2' => ( |
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is => 'ro', |
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isa => 'Bio::MUST::Core::Ali', |
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required => 1, |
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coerce => 1, |
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); |
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has 'ali' => ( |
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is => 'ro', |
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isa => 'Bio::MUST::Core::Ali', |
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init_arg => undef, |
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writer => '_set_ali', |
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handles => qr{.*}xms, |
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); |
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sub BUILD { |
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my $self = shift; |
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my $ali1 = $self->file1; |
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my $ali2 = $self->file2; |
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my ($filename1, $id_mapper1) = $ali1->temp_fasta( {id_prefix => 'file1-'} ); |
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my ($filename2, $id_mapper2) = $ali2->temp_fasta( {id_prefix => 'file2-'} ); |
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my %mapper = ( ali1 => $id_mapper1, ali2 => $id_mapper2 ); |
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my $mafft = Mafft->new( file => $filename1 ); # add an option to use clustalo if wanted ? |
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my $ali_out = $mafft->seqs2profile($filename2); |
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$ali_out->dont_guess; |
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$ali_out->restore_ids($mapper{ali1}); |
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$ali_out->restore_ids($mapper{ali2}); |
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$self->_set_ali($ali_out); |
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return; |
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} |
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__PACKAGE__->meta->make_immutable; |
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1; |
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__END__ |
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=pod |
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=head1 NAME |
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Bio::MUST::Apps::TwoScalp::Seqs2Profile - internal class for two-scalp tool |
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=head1 VERSION |
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version 0.201810 |
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=head1 SYNOPSIS |
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# TODO |
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=head1 DESCRIPTION |
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# TODO |
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=head1 AUTHOR |
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Denis BAURAIN <denis.baurain@uliege.be> |
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=head1 CONTRIBUTOR |
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=for stopwords Amandine BERTRAND |
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Amandine BERTRAND <amandine.bertrand@doct.uliege.be> |
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=head1 COPYRIGHT AND LICENSE |
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This software is copyright (c) 2013 by University of Liege / Unit of Eukaryotic Phylogenomics / Denis BAURAIN. |
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This is free software; you can redistribute it and/or modify it under |
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the same terms as the Perl 5 programming language system itself. |
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=cut |