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package Bio::DOOP::SequenceFeature; |
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use strict; |
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use warnings; |
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923
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=head1 NAME |
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Bio::DOOP::SequenceFeature - Object for the sequence features |
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=head1 VERSION |
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Version 0.10 |
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=cut |
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our $VERSION = '0.10'; |
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=head1 SYNOPSIS |
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my $seqfeat = Bio::DOOP::SequenceFeature->new($db,"112"); |
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my $type = $seqfeat->get_type; |
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=head1 DESCRIPTION |
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This object gives access to the sequence features (conserved motif, repeat, CpG island, |
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TFBS and TSS annotation). |
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=head1 AUTHORS |
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30
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Tibor Nagy, Godollo, Hungary and Endre Sebestyen, Martonvasar, Hungary |
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=head1 METHODS |
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=head2 new |
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35
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Creates a new sequence feature object from the sequence feature primary id. |
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38
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Return type: Bio::DOOP::SequenceFeature object |
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39
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40
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$seqfeat = Bio::DOOP::SequenceFeature->new($db,"112"); |
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42
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=cut |
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43
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44
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sub new { |
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45
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0
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1
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my $self = {}; |
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46
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0
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my $dummy = shift; |
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47
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0
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my $db = shift; |
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48
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0
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my $id = shift; |
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49
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50
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0
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my $ret = $db->query("SELECT * FROM sequence_feature WHERE sequence_feature_primary_id = \"$id\";"); |
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51
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52
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0
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0
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if ($#$ret == -1) { |
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53
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0
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return(-1); |
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54
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} |
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55
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0
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my @fields = @{$$ret[0]}; |
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0
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56
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57
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0
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$self->{PRIMARY} = $fields[0]; |
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58
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0
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$self->{SCORE} = $fields[1]; |
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59
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0
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$self->{START} = $fields[2]; |
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60
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0
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$self->{END} = $fields[3]; |
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61
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0
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$self->{SEQ} = $fields[4]; |
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62
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0
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$self->{TYPE} = $fields[5]; |
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63
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0
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$self->{MOTIFID} = $fields[6]; |
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64
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0
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$self->{TFBSID} = $fields[7]; |
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65
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0
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$self->{CPGID} = $fields[8]; |
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66
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0
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$self->{REPEATID} = $fields[9]; |
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67
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0
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$self->{SSRID} = $fields[10]; |
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68
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0
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$self->{TSSID} = $fields[11]; |
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69
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0
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$self->{SUBSET_ID} = $fields[12]; |
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70
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0
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$self->{SEQ_ID} = $fields[13]; |
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71
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72
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0
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0
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if ($self->{TYPE} eq "ssr"){ |
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0
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0
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0
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0
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0
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73
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0
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$ret = $db->query("SELECT * FROM ssr_annotation WHERE ssr_primary_id = \"".$self->{SSRID}."\";"); |
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74
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0
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@fields = @{$$ret[0]}; |
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0
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75
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0
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$self->{SSRUNIT} = $fields[1]; |
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76
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} |
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77
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elsif ($self->{TYPE} eq "rep"){ |
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78
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0
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$ret = $db->query("SELECT * FROM repeat_annotation WHERE repeat_primary_id = \"".$self->{REPEATID}."\";"); |
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79
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0
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@fields = @{$$ret[0]}; |
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0
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80
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0
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$self->{R_NAME} = $fields[1]; |
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81
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0
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$self->{R_CLASS} = $fields[2]; |
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82
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0
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$self->{R_DESC} = $fields[3]; |
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83
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0
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$self->{R_XREF} = $fields[4]; |
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84
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} |
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85
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elsif ($self->{TYPE} eq "tfbs"){ |
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86
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0
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$ret = $db->query("SELECT * FROM tfbs_annotation WHERE tfbs_primary_id = \"".$self->{TFBSID}."\";"); |
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87
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0
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@fields = @{$$ret[0]}; |
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0
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88
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0
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$self->{TF_NAME} = $fields[1]; |
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89
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0
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$self->{TF_ORIG} = $fields[2]; |
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90
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0
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$self->{TF_DESC} = $fields[3]; |
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91
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0
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$self->{TF_TYPE} = $fields[4]; |
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92
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0
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$self->{TF_CONS} = $fields[5]; |
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93
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0
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$self->{TF_MTRX} = $fields[6]; |
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94
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0
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$self->{TF_XREF} = $fields[7]; |
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95
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} |
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96
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elsif ($self->{TYPE} eq "cpg"){ |
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97
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0
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$ret = $db->query("SELECT * FROM cpg_annotation WHERE cpg_primary_id = \"".$self->{CPGID}."\";"); |
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98
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0
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@fields = @{$$ret[0]}; |
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0
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99
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0
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$self->{CPG_P} = $fields[1]; |
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100
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} |
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101
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elsif ($self->{TYPE} eq "tss"){ |
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102
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0
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$ret = $db->query("SELECT * FROM tss_annotation WHERE tss_primary_id = \"".$self->{TSSID}."\";"); |
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103
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0
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@fields = @{$$ret[0]}; |
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0
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104
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0
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$self->{T_TYPE} = $fields[1]; |
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105
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0
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$self->{T_ID} = $fields[2]; |
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106
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0
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$self->{T_DESC} = $fields[3]; |
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107
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0
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$self->{T_XREF} = $fields[4]; |
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108
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} |
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109
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elsif ($self->{TYPE} eq "con"){ |
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110
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0
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my $motif = Bio::DOOP::Motif->new($db,$self->{MOTIFID}); |
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111
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0
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$self->{MOTIF} = $motif; |
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112
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} |
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113
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114
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0
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bless $self; |
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115
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0
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return($self); |
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116
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} |
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117
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118
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=head2 get_id |
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119
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120
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Returns the primary ID of the feature. This is the internal ID from the MySQL database. |
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121
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122
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=cut |
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123
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124
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sub get_id { |
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my $self = shift; |
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return($self->{PRIMARY}); |
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} |
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128
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129
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=head2 get_score |
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131
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This method is not yet implemented. |
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133
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=cut |
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134
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135
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sub get_score { |
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my $self = shift; |
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0
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return($self->{SCORE}); |
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} |
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139
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140
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=head2 get_start |
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141
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142
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Returns the start position of the feature. |
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143
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144
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=cut |
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145
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146
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sub get_start { |
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147
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0
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1
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my $self = shift; |
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148
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0
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return($self->{START}); |
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149
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} |
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150
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151
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=head2 get_end |
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152
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153
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Returns the end position of the feature. |
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154
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155
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=cut |
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156
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157
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sub get_end { |
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158
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my $self = shift; |
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159
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0
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return($self->{END}); |
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160
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} |
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161
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162
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=head2 length |
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163
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164
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Returns the length of the feature. |
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165
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166
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=cut |
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167
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168
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sub length { |
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169
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0
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0
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1
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my $self = shift; |
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170
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0
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return($self->{END} - $self->{START} + 1); |
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171
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} |
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172
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173
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=head2 get_seq |
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174
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175
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Returns the sequence of the feature. |
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176
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177
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=cut |
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178
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179
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sub get_seq { |
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180
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0
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0
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1
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my $self = shift; |
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181
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0
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return($self->{SEQ}); |
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182
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} |
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183
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184
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=head2 get_type |
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185
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186
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Returns the type of the feature. (con, ssr, tfbs, rep, cpg, tss). |
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187
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188
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con : evolutionary conserved non-coding region |
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189
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ssr : simple sequence repeat |
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190
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tfbs : transcription factor binding site |
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191
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rep : repeat |
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192
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cpg : cpg island |
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193
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tss : transcription start site |
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194
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195
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=cut |
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196
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197
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sub get_type { |
|
198
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0
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0
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1
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my $self = shift; |
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199
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0
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return($self->{TYPE}); |
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200
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} |
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201
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202
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=head2 get_motifid |
|
203
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204
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Returns the motif primary ID, if the feature type is "con". |
|
205
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206
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=cut |
|
207
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208
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sub get_motifid { |
|
209
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0
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0
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1
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|
my $self = shift; |
|
210
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0
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return($self->{MOTIFID}); |
|
211
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} |
|
212
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213
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=head2 get_tfbsid |
|
214
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215
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Returns the tfbs primary ID, if the feature type is "tfbs". |
|
216
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217
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=cut |
|
218
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219
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sub get_tfbsid { |
|
220
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0
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0
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1
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|
my $self = shift; |
|
221
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0
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return($self->{TFBSID}); |
|
222
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} |
|
223
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224
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=head2 get_cpgid |
|
225
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226
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Returns the cpg primary ID, if the feature type is "cpg". |
|
227
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228
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=cut |
|
229
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230
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sub get_cpgid { |
|
231
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0
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0
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1
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|
my $self = shift; |
|
232
|
0
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|
return($self->{CPGID}); |
|
233
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} |
|
234
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235
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=head2 get_repeatid |
|
236
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237
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|
Returns the repeat primary ID, if the feature type is "rep". |
|
238
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|
239
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=cut |
|
240
|
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|
241
|
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|
sub get_repeatid { |
|
242
|
0
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|
|
0
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1
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|
my $self = shift; |
|
243
|
0
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|
return($self->{REPEATID}); |
|
244
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} |
|
245
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246
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|
=head2 get_ssrid |
|
247
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|
248
|
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|
Returns the ssr primary ID, if the feature type is "ssr". |
|
249
|
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|
250
|
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|
=cut |
|
251
|
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|
252
|
|
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|
sub get_ssrid { |
|
253
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
|
254
|
0
|
|
|
|
|
|
return($self->{SSRID}); |
|
255
|
|
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|
|
} |
|
256
|
|
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|
257
|
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|
=head2 get_tssid |
|
258
|
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|
259
|
|
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|
|
|
Returns the tss primary ID, if the feature type is "tss". |
|
260
|
|
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|
261
|
|
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|
|
|
|
=cut |
|
262
|
|
|
|
|
|
|
|
|
263
|
|
|
|
|
|
|
sub get_tssid { |
|
264
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
|
265
|
0
|
|
|
|
|
|
return($self->{TSSID}); |
|
266
|
|
|
|
|
|
|
} |
|
267
|
|
|
|
|
|
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|
|
268
|
|
|
|
|
|
|
=head2 get_seqid |
|
269
|
|
|
|
|
|
|
|
|
270
|
|
|
|
|
|
|
Returns the primary ID of the sequence containing this feature. |
|
271
|
|
|
|
|
|
|
|
|
272
|
|
|
|
|
|
|
=cut |
|
273
|
|
|
|
|
|
|
|
|
274
|
|
|
|
|
|
|
sub get_seqid { |
|
275
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
|
276
|
0
|
|
|
|
|
|
return($self->{SEQ_ID}); |
|
277
|
|
|
|
|
|
|
} |
|
278
|
|
|
|
|
|
|
|
|
279
|
|
|
|
|
|
|
=head2 get_subsetid |
|
280
|
|
|
|
|
|
|
|
|
281
|
|
|
|
|
|
|
Returns the subset primary ID of the feature, if the feature type is "con". |
|
282
|
|
|
|
|
|
|
|
|
283
|
|
|
|
|
|
|
=cut |
|
284
|
|
|
|
|
|
|
|
|
285
|
|
|
|
|
|
|
sub get_subsetid { |
|
286
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
|
287
|
0
|
|
|
|
|
|
return($self->{SUBSET_ID}); |
|
288
|
|
|
|
|
|
|
} |
|
289
|
|
|
|
|
|
|
|
|
290
|
|
|
|
|
|
|
=head2 get_motif |
|
291
|
|
|
|
|
|
|
|
|
292
|
|
|
|
|
|
|
Returns the motif object associated with the feature. |
|
293
|
|
|
|
|
|
|
If the feature type is not "con" this value is NULL. |
|
294
|
|
|
|
|
|
|
|
|
295
|
|
|
|
|
|
|
=cut |
|
296
|
|
|
|
|
|
|
|
|
297
|
|
|
|
|
|
|
sub get_motif { |
|
298
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
|
299
|
0
|
|
|
|
|
|
return($self->{MOTIF}); |
|
300
|
|
|
|
|
|
|
} |
|
301
|
|
|
|
|
|
|
|
|
302
|
|
|
|
|
|
|
=head2 get_tss_type |
|
303
|
|
|
|
|
|
|
|
|
304
|
|
|
|
|
|
|
Returns the tss type, if the feature type is "tss". |
|
305
|
|
|
|
|
|
|
|
|
306
|
|
|
|
|
|
|
=cut |
|
307
|
|
|
|
|
|
|
|
|
308
|
|
|
|
|
|
|
sub get_tss_type { |
|
309
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
|
310
|
0
|
|
|
|
|
|
return($self->{T_TYPE}); |
|
311
|
|
|
|
|
|
|
} |
|
312
|
|
|
|
|
|
|
|
|
313
|
|
|
|
|
|
|
=head2 get_tss_id |
|
314
|
|
|
|
|
|
|
|
|
315
|
|
|
|
|
|
|
Returns the tss id, if the feature type is "tss". |
|
316
|
|
|
|
|
|
|
|
|
317
|
|
|
|
|
|
|
=cut |
|
318
|
|
|
|
|
|
|
|
|
319
|
|
|
|
|
|
|
sub get_tss_id { |
|
320
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
|
321
|
0
|
|
|
|
|
|
return($self->{T_ID}); |
|
322
|
|
|
|
|
|
|
} |
|
323
|
|
|
|
|
|
|
|
|
324
|
|
|
|
|
|
|
=head2 get_tss_desc |
|
325
|
|
|
|
|
|
|
|
|
326
|
|
|
|
|
|
|
Returns the description of the tss, if the feature type is "tss". |
|
327
|
|
|
|
|
|
|
|
|
328
|
|
|
|
|
|
|
=cut |
|
329
|
|
|
|
|
|
|
|
|
330
|
|
|
|
|
|
|
sub get_tss_desc { |
|
331
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
|
332
|
0
|
|
|
|
|
|
return($self->{T_DESC}); |
|
333
|
|
|
|
|
|
|
} |
|
334
|
|
|
|
|
|
|
|
|
335
|
|
|
|
|
|
|
=head2 get_tss_xref |
|
336
|
|
|
|
|
|
|
|
|
337
|
|
|
|
|
|
|
Returns the xref of the tss, if the feature type is "tss". |
|
338
|
|
|
|
|
|
|
|
|
339
|
|
|
|
|
|
|
=cut |
|
340
|
|
|
|
|
|
|
|
|
341
|
|
|
|
|
|
|
sub get_tss_xref { |
|
342
|
0
|
|
|
0
|
1
|
|
my $self = shift; |
|
343
|
0
|
|
|
|
|
|
return($self->{T_XREF}); |
|
344
|
|
|
|
|
|
|
} |
|
345
|
|
|
|
|
|
|
|
|
346
|
|
|
|
|
|
|
1; |