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| 1 |  |  |  |  |  |  | package App::SimulateReads::Fastq; | 
| 2 |  |  |  |  |  |  | # ABSTRACT: Base class to simulate fastq entries | 
| 3 |  |  |  |  |  |  |  | 
| 4 | 6 |  |  | 6 |  | 3309 | use App::SimulateReads::Base 'class'; | 
|  | 6 |  |  |  |  | 13 |  | 
|  | 6 |  |  |  |  | 51 |  | 
| 5 | 6 |  |  | 6 |  | 2324 | use App::SimulateReads::Quality; | 
|  | 6 |  |  |  |  | 2761 |  | 
|  | 6 |  |  |  |  | 1599 |  | 
| 6 |  |  |  |  |  |  |  | 
| 7 |  |  |  |  |  |  | our $VERSION = '0.05'; # VERSION | 
| 8 |  |  |  |  |  |  |  | 
| 9 |  |  |  |  |  |  | #------------------------------------------------------------------------------- | 
| 10 |  |  |  |  |  |  | #  Moose attributes | 
| 11 |  |  |  |  |  |  | #------------------------------------------------------------------------------- | 
| 12 |  |  |  |  |  |  | has 'quality_profile'   => (is => 'ro', isa => 'My:QualityP', required => 1, coerce => 1); | 
| 13 |  |  |  |  |  |  | has 'read_size'         => (is => 'ro', isa => 'My:IntGt0',   required => 1); | 
| 14 |  |  |  |  |  |  | has '_quality'          => ( | 
| 15 |  |  |  |  |  |  | is         => 'ro', | 
| 16 |  |  |  |  |  |  | isa        => 'App::SimulateReads::Quality', | 
| 17 |  |  |  |  |  |  | builder    => '_build_quality', | 
| 18 |  |  |  |  |  |  | lazy_build => 1, | 
| 19 |  |  |  |  |  |  | handles    => [qw{ gen_quality }] | 
| 20 |  |  |  |  |  |  | ); | 
| 21 |  |  |  |  |  |  |  | 
| 22 |  |  |  |  |  |  | sub BUILD { | 
| 23 | 28 |  |  | 28 | 0 | 56 | my $self = shift; | 
| 24 |  |  |  |  |  |  | ## Just to ensure that the lazy attributes are built before &new returns | 
| 25 | 28 |  |  |  |  | 936 | $self->_quality; | 
| 26 |  |  |  |  |  |  | } | 
| 27 |  |  |  |  |  |  |  | 
| 28 |  |  |  |  |  |  | #===  CLASS METHOD  ============================================================ | 
| 29 |  |  |  |  |  |  | #        CLASS: Fast | 
| 30 |  |  |  |  |  |  | #       METHOD: _build_quality (BUILDER) | 
| 31 |  |  |  |  |  |  | #   PARAMETERS: Void | 
| 32 |  |  |  |  |  |  | #      RETURNS: Quality obj | 
| 33 |  |  |  |  |  |  | #  DESCRIPTION: Build Quality object | 
| 34 |  |  |  |  |  |  | #       THROWS: no exceptions | 
| 35 |  |  |  |  |  |  | #     COMMENTS: none | 
| 36 |  |  |  |  |  |  | #     SEE ALSO: n/a | 
| 37 |  |  |  |  |  |  | #=============================================================================== | 
| 38 |  |  |  |  |  |  | sub _build_quality { | 
| 39 | 28 |  |  | 28 |  | 59 | my $self = shift; | 
| 40 | 28 |  |  |  |  | 833 | App::SimulateReads::Quality->new( | 
| 41 |  |  |  |  |  |  | quality_profile => $self->quality_profile, | 
| 42 |  |  |  |  |  |  | read_size       => $self->read_size | 
| 43 |  |  |  |  |  |  | ); | 
| 44 |  |  |  |  |  |  | } ## --- end sub _build_quality | 
| 45 |  |  |  |  |  |  |  | 
| 46 |  |  |  |  |  |  | #===  CLASS METHOD  ============================================================ | 
| 47 |  |  |  |  |  |  | #        CLASS: Fast | 
| 48 |  |  |  |  |  |  | #       METHOD: sprintf_fastq | 
| 49 |  |  |  |  |  |  | #   PARAMETERS: $header_ref Ref Str, $seq_ref Ref Str | 
| 50 |  |  |  |  |  |  | #      RETURNS: $fastq Ref Str | 
| 51 |  |  |  |  |  |  | #  DESCRIPTION: Fastq entry template | 
| 52 |  |  |  |  |  |  | #       THROWS: no exceptions | 
| 53 |  |  |  |  |  |  | #     COMMENTS: none | 
| 54 |  |  |  |  |  |  | #     SEE ALSO: n/a | 
| 55 |  |  |  |  |  |  | #=============================================================================== | 
| 56 |  |  |  |  |  |  | sub fastq_template { | 
| 57 | 2289 |  |  | 2289 | 0 | 7259 | my ($self, $header_ref, $seq_ref) = @_; | 
| 58 | 2289 |  |  |  |  | 6421 | my $quality_ref = $self->gen_quality; | 
| 59 |  |  |  |  |  |  |  | 
| 60 | 2289 |  |  |  |  | 6686 | my $fastq = "\@$$header_ref\n"; | 
| 61 | 2289 |  |  |  |  | 6154 | $fastq .= "$$seq_ref\n"; | 
| 62 | 2289 |  |  |  |  | 3645 | $fastq .= "+\n"; | 
| 63 | 2289 |  |  |  |  | 3523 | $fastq .= "$$quality_ref"; | 
| 64 |  |  |  |  |  |  |  | 
| 65 | 2289 |  |  |  |  | 9597 | return \$fastq; | 
| 66 |  |  |  |  |  |  | } ## --- end sub fastq_template | 
| 67 |  |  |  |  |  |  |  | 
| 68 |  |  |  |  |  |  | __END__ | 
| 69 |  |  |  |  |  |  |  | 
| 70 |  |  |  |  |  |  | =pod | 
| 71 |  |  |  |  |  |  |  | 
| 72 |  |  |  |  |  |  | =encoding UTF-8 | 
| 73 |  |  |  |  |  |  |  | 
| 74 |  |  |  |  |  |  | =head1 NAME | 
| 75 |  |  |  |  |  |  |  | 
| 76 |  |  |  |  |  |  | App::SimulateReads::Fastq - Base class to simulate fastq entries | 
| 77 |  |  |  |  |  |  |  | 
| 78 |  |  |  |  |  |  | =head1 VERSION | 
| 79 |  |  |  |  |  |  |  | 
| 80 |  |  |  |  |  |  | version 0.05 | 
| 81 |  |  |  |  |  |  |  | 
| 82 |  |  |  |  |  |  | =head1 AUTHOR | 
| 83 |  |  |  |  |  |  |  | 
| 84 |  |  |  |  |  |  | Thiago L. A. Miller <tmiller@mochsl.org.br> | 
| 85 |  |  |  |  |  |  |  | 
| 86 |  |  |  |  |  |  | =head1 COPYRIGHT AND LICENSE | 
| 87 |  |  |  |  |  |  |  | 
| 88 |  |  |  |  |  |  | This software is Copyright (c) 2017 by Teaching and Research Institute from SÃrio-Libanês Hospital. | 
| 89 |  |  |  |  |  |  |  | 
| 90 |  |  |  |  |  |  | This is free software, licensed under: | 
| 91 |  |  |  |  |  |  |  | 
| 92 |  |  |  |  |  |  | The GNU General Public License, Version 3, June 2007 | 
| 93 |  |  |  |  |  |  |  | 
| 94 |  |  |  |  |  |  | =cut |