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package App::Sandy; |
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# ABSTRACT: App builder that simulates single-end and paired-end reads. |
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use App::Sandy::Base 'class'; |
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extends 'App::Sandy::CLI::App'; |
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our $VERSION = '0.25'; # VERSION |
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sub command_map { |
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genome => 'App::Sandy::Command::Genome', |
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transcriptome => 'App::Sandy::Command::Transcriptome', |
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quality => 'App::Sandy::Command::Quality', |
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expression => 'App::Sandy::Command::Expression', |
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variation => 'App::Sandy::Command::Variation', |
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version => 'App::Sandy::Command::Version', |
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citation => 'App::Sandy::Command::Citation' |
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} |
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__END__ |
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=pod |
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=encoding UTF-8 |
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=head1 NAME |
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App::Sandy - App builder that simulates single-end and paired-end reads. |
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=head1 VERSION |
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version 0.25 |
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=head1 SYNOPSIS |
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sandy [options] |
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sandy help <command> |
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sandy <command> ... |
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Options: |
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-h, --help brief help message |
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-H, --man full documentation |
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Help commands: |
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help show application or command-specific help |
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man show application or command-specific documentation |
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Misc commands: |
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version print the current version |
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citation export citation in BibTeX format |
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Database commands: |
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quality manage quality profile database |
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expression manage expression-matrix database |
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variation manage structural variation database |
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Main commands: |
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genome simulate genome sequencing |
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transcriptome simulate transcriptome sequencing |
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=head1 DESCRIPTION |
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B<Sandy> is a bioinformatics tool that provides a simple engine to simulate next-generation |
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sequencing for genomic and transcriptomic data. Simulated data works as experimental control |
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- a key step to optimize NGS analysis - in comparison to hypothetical models. B<Sandy> is a |
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straightforward, easy-to-use, fast and highly customizable tool that generates reads requiring |
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only a FASTA file as input. B<Sandy> can simulate single/paired-end reads from both whole exome |
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sequencing and RNA-seq as if produced from the most used second and third-generation sequencing |
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platforms. B<Sandy>’s reads can be simulated with genetic variations such as SNVs, indels and gene |
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fusions. For customization purposes, B<Sandy> have built-in (native) databases that can be easily |
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extended with varying gene/transcript expression profiles, sequencing errors, sequencing |
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coverages and genomic variations. |
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=head1 AUTHORS |
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=over 4 |
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=item * |
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Thiago L. A. Miller <tmiller@mochsl.org.br> |
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=item * |
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J. Leonel Buzzo <lbuzzo@mochsl.org.br> |
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=item * |
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Felipe R. C. dos Santos <fsantos@mochsl.org.br> |
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=item * |
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Helena B. Conceição <hconceicao@mochsl.org.br> |
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=item * |
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Rodrigo Barreiro <rbarreiro@mochsl.org.br> |
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=item * |
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Gabriela Guardia <gguardia@mochsl.org.br> |
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=item * |
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Fernanda Orpinelli <forpinelli@mochsl.org.br> |
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=item * |
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Rafael Mercuri <rmercuri@mochsl.org.br> |
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=item * |
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Rodrigo Barreiro <rbarreiro@mochsl.org.br> |
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=item * |
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Pedro A. F. Galante <pgalante@mochsl.org.br> |
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=back |
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=head1 COPYRIGHT AND LICENSE |
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This software is Copyright (c) 2023 by Teaching and Research Institute from Sírio-Libanês Hospital. |
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This is free software, licensed under: |
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The GNU General Public License, Version 3, June 2007 |
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=cut |