File Coverage

lib/App/Sandy/DB/Handle/Quality.pm

Criterion	Covered	Total	%
statement	18	171	10.5
branch	0	50	0.0
condition	0	18	0.0
subroutine	6	16	37.5
pod	0	5	0.0
total	24	260	9.2

package App::Sandy::DB::Handle::Quality;
# ABSTRACT: Class to handle quality database schemas.

use App::Sandy::Base 'class';
use App::Sandy::DB;
use IO::Compress::Gzip 'gzip';
use IO::Uncompress::Gunzip 'gunzip';
use Storable qw/nfreeze thaw/;

use constant {
	SEED            => 1717,
	PARTIL          => 10,
	DEFAULT_PROFILE => {
		poisson => {
			'mean'     => '-m',
			'stdd'     => '-d',
			'error'    => '-e',
			'type'     => 'both',
			'source'   => 'default',
			'provider' => 'vendor',
			'date'     => '2018-08-08'
		}
	}
};

with qw{
	App::Sandy::Role::IO
	App::Sandy::Role::Statistic
	App::Sandy::Role::Counter
};

our $VERSION = '0.25'; # VERSION

sub insertdb {
	my ($self, $file, $name, $source, $is_user_provided, $error, $single_molecule, $type) = @_;
	my $schema = App::Sandy::DB->schema;
	my %default_profile = %{ &DEFAULT_PROFILE };

	log_msg ":: Checking if there is already a quality-profile '$name' ...";
	my $rs = $schema->resultset('QualityProfile')->find({ name => $name });
	if ($rs || $default_profile{$name}) {
		die "There is already a quality-profile '$name'\n";
	} else {
		log_msg ":: quality-profile '$name' not found";
	}

	if ($type !~ /^(fastq|raw)$/) {
		die "Unknown indexing type '$type': Valids are 'raw' and 'fastq'\n";
	}

	log_msg ":: Indexing '$file'. It may take several minutes ...";
	my ($arr, $mean, $stdd, $deepth) = $self->_index_quality_type($file, $type);

	log_msg ":: Converting array to bytes ...";
	my $bytes = nfreeze $arr;
	log_msg ":: Compressing bytes ...";
	gzip \$bytes => \my $compressed;

	# Begin transation
	my $guard = $schema->txn_scope_guard;

	log_msg ":: Storing quality matrix entry at '$name' ...";
	$rs = $schema->resultset('QualityProfile')->create({
		'name'               => $name,
		'source'             => $source,
		'is_user_provided'   => $is_user_provided,
		'is_single_molecule' => $single_molecule,
		'mean'               => $mean,
		'stdd'               => $stdd,
		'error'              => $error,
		'deepth'             => $deepth,
		'partil'             => PARTIL,
		'matrix'             => $compressed
	});

	# End transation
	$guard->commit;
}

sub _index_quality {
	my ($self, $quality_ref, $rng) = @_;
	my (@partil, @sizes);

	for my $entry (@$quality_ref) {
		my @q = split // => $entry;

		my $size = scalar @q;
		push @sizes => $size;

		my $bin = int($size / PARTIL);
		my $left = $size % PARTIL;

		my $pos = 0;
		my $skip = $self->with_make_counter($size - $left, $left, $rng);

		for (my $i = 0; $i < PARTIL; $i++) {
			for (my $j = 0; $j < $bin; $j++) {
				$pos++ if $skip->();
				push @{ $partil[$i] } => $q[$pos++];
			}
		}
	}

	my $deepth = scalar @{ $partil[0] };
	for (my $i = 1; $i < PARTIL; $i++) {
		if ($deepth != scalar(@{ $partil[$i] })) {
			croak "deepth differs at partil $i";
		}
	}

	# Basic stats
	my $mean = int($self->with_mean(\@sizes) + 0.5);
	my $stdd = int($self->with_stdd(\@sizes) + 0.5);

	return (\@partil, $mean, $stdd, $deepth);
}

sub _index_quality_type {
	my ($self, $file, $type) = @_;
	my $fh = $self->with_open_r($file);

	log_msg ":: Counting number of lines in '$file' ...";
	my $num_lines = $self->_wcl($file);

	if ($num_lines == 0) {
		die "The file '$file' is empty\n";
	}

	log_msg ":: Number of lines: $num_lines";

	my $num_left = 0;
	my $line = 0;
	my $acm = 0;

	my $getter;

	if ($type eq 'fastq') {
		log_msg ":: Setting fastq validation and getter";
		$num_left = int($num_lines / 4);

		$getter = sub {
			return if eof($fh);

			my @stack;

			for (1..4) {
				$line++;
				defined(my $entry = <$fh>)
					or die "Truncated fastq entry in '$file' at line $line\n";
				push @stack => $entry;
			}

			chomp @stack;

			if ($stack[0] !~ /^\@/ || $stack[2] !~ /^\+/) {
				die "Fastq entry at '$file' line '", $line - 3, "' not seems to be a valid read\n";
			}

			return $stack[3];
		};
	} else {
		log_msg ":: Setting raw validation and getter";
		$num_left = $num_lines;

		$getter = sub {
			defined(my $entry = <$fh>)
				or return;

			$line++;
			chomp $entry;

			return $entry;
		};
	}

	log_msg ":: Calculating the  number of entries to pick ...";
	my $picks = $num_left < 1000 ? $num_left : 1000;
	my $picks_left = $picks;

	my $rng = App::Sandy::RNG->new(SEED);
	my $do_pick = $self->with_make_counter($num_left, $picks, $rng);
	my @quality;

	log_msg ":: Picking $picks entries in '$file' ...";
	while (my $entry = $getter->()) {
		if ($do_pick->()) {
			push @quality => $entry if length($entry) >= PARTIL;
			if ($picks >= 10 && (++$acm % int($picks/10) == 0)) {
				log_msg sprintf "   ==> %d%% processed", ($acm / $picks) * 100;
			}
			last if --$picks_left <= 0;
		}
	}

	if (scalar(@quality) == 0) {
		die "All quality entries have length lesser then 10 in file '$file'.\n" .
			"quality-profile constraints the quality length to values greater or equal to 10.\n" .
			"If you need somehow profiles with length lesser than 10. Please use --quality-profile=poisson\n";
	}

	$fh->close
		or die "Cannot close file '$file'\n";

	return $self->_index_quality(\@quality, $rng);
}

sub _wcl {
	my ($self, $file) = @_;
	my $fh = $self->with_open_r($file);
	my $num_lines = 0;
	$num_lines++ while <$fh>;
	$fh->close;
	return $num_lines;
}

sub retrievedb {
	my ($self, $quality_profile) = @_;
	my $schema = App::Sandy::DB->schema;
	my %default_profile = %{ &DEFAULT_PROFILE };

	if ($default_profile{$quality_profile}) {
		die "Cannot retrieve '$quality_profile' because it is based on a theoretical distribution\n";
	}

	my $rs = $schema->resultset('QualityProfile')->find({ name => $quality_profile });
	die "'$quality_profile' not found into database\n" unless defined $rs;

	my $compressed = $rs->matrix;
	die "quality-profile entry '$quality_profile' exists, but the related data is missing\n"
		unless defined $compressed;

	my $deepth = $rs->deepth;
	my $partil = $rs->partil;

	gunzip \$compressed => \my $bytes;
	my $matrix = thaw $bytes;
	return ($matrix, $deepth, $partil);
}

sub deletedb {
	my ($self, $quality_profile) = @_;
	my $schema = App::Sandy::DB->schema;
	my %default_profile = %{ &DEFAULT_PROFILE };

	log_msg ":: Checking if there is a quality-profile '$quality_profile' ...";
	my $rs = $schema->resultset('QualityProfile')->find({ name => $quality_profile });

	unless ($rs || $default_profile{$quality_profile}) {
		die "'$quality_profile' not found into database\n";
	}

	log_msg ":: Found '$quality_profile'";

	if (($rs && !$rs->is_user_provided) || $default_profile{$quality_profile}) {
		die "'$quality_profile' is not a user provided entry. Cannot be deleted\n";
	}

	log_msg ":: Removing '$quality_profile' entry ...";

	# Begin transation
	my $guard = $schema->txn_scope_guard;

	$rs->delete;

	# End transation
	$guard->commit;
}

sub restoredb {
	my $self = shift;
	my $schema = App::Sandy::DB->schema;

	log_msg ":: Searching for user-provided entries ...";
	my $user_provided = $schema->resultset('QualityProfile')->search(
		{ is_user_provided => 1 },
	);

	my $entry = $user_provided->next;
	if ($entry) {
		log_msg ":: Found:";
		do {
			log_msg '   ==> ' . $entry->name;
		} while ($entry = $user_provided->next);
	} else {
		die "Not found user-provided entries. There is no need to restoring\n";
	}

	log_msg ":: Removing all user-provided entries ...";

	# Begin transation
	my $guard = $schema->txn_scope_guard;

	$user_provided->delete;

	# End transation
	$guard->commit;
}

sub make_report {
	my $self = shift;
	my $schema = App::Sandy::DB->schema;
	my %report = %{ &DEFAULT_PROFILE };

	my $rs = $schema->resultset('QualityProfile')->search(undef);

	while (my $quality = $rs->next) {
		my %hash = (
			'mean'       => $quality->mean,
			'stdd'       => $quality->stdd,
			'error'      => $quality->error,
			'type'       => $quality->is_single_molecule ? 'single-molecule' : 'fragment',
			'source'     => $quality->source,
			'provider'   => $quality->is_user_provided ? "user" : "vendor",
			'date'       => $quality->date
		);
		$report{$quality->name} = \%hash;
	}

	return \%report;
}

__END__

=pod

=encoding UTF-8

=head1 NAME

App::Sandy::DB::Handle::Quality - Class to handle quality database schemas.

=head1 VERSION

version 0.25

=head1 AUTHORS

=over 4

=item *

Thiago L. A. Miller <[e-mail]>

=item *

J. Leonel Buzzo <[e-mail]>

=item *

Felipe R. C. dos Santos <[e-mail]>

=item *

Helena B. Conceição <[e-mail]>

=item *

Rodrigo Barreiro <[e-mail]>

=item *

Gabriela Guardia <[e-mail]>

=item *

Fernanda Orpinelli <[e-mail]>

=item *

Rafael Mercuri <[e-mail]>

=item *

Rodrigo Barreiro <[e-mail]>

=item *

Pedro A. F. Galante <[e-mail]>

=back

=head1 COPYRIGHT AND LICENSE

This software is Copyright (c) 2023 by Teaching and Research Institute from Sírio-Libanês Hospital.

This is free software, licensed under:

  The GNU General Public License, Version 3, June 2007

=cut