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package App::Rangeops::Command::circos; |
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68063
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use strict; |
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249
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43
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use warnings; |
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3150
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use autodie; |
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103451
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55545
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use App::Rangeops -command; |
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107
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7740
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use App::Rangeops::Common; |
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use constant abstract => 'range links to circos links or highlight file'; |
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6210
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11
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sub opt_spec { |
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return ( |
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3
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3
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1
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17
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[ "outfile|o=s", "Output filename. [stdout] for screen." ], |
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[ "highlight|l", "Create highlights instead of links", ], |
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); |
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} |
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sub usage_desc { |
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29259
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return "rangeops circos [options] "; |
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} |
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sub description { |
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1
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1
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1
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643
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my $desc; |
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1
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$desc .= ucfirst(abstract) . ".\n"; |
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1
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$desc .= "\tIt's assumed that all ranges in input files are valid.\n"; |
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1
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return $desc; |
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} |
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sub validate_args { |
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1716
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my ( $self, $opt, $args ) = @_; |
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2
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50
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if ( !@{$args} ) { |
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2
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7
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0
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0
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$self->usage_error("This command need one or more input files."); |
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} |
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2
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5
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for ( @{$args} ) { |
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2
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5
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36
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2
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next if lc $_ eq "stdin"; |
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2
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10
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if ( !Path::Tiny::path($_)->is_file ) { |
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0
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0
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$self->usage_error("The input file [$_] doesn't exist."); |
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} |
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} |
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2
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173
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if ( !exists $opt->{outfile} ) { |
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$opt->{outfile} |
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0
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0
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= Path::Tiny::path( $args->[0] )->absolute . ".links.txt"; |
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} |
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} |
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48
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sub execute { |
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2
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2
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1
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12
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my ( $self, $opt, $args ) = @_; |
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50
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51
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#----------------------------# |
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52
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# Loading |
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53
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#----------------------------# |
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54
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2
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4
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my @lines; |
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55
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2
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5
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for my $file ( @{$args} ) { |
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2
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5
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56
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2
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15
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for my $line ( App::RL::Common::read_lines($file) ) { |
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57
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12
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2568
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my @parts = split /\t/, $line; |
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58
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12
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27
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my @colors = reverse map {"paired-12-qual-$_"} ( 1 .. 12 ); |
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144
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318
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59
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12
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27
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my $color_idx = 0; |
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60
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61
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12
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100
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34
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if ( defined $opt->{highlight} ) { |
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62
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63
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6
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60
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for my $part (@parts) { |
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64
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14
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45
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my $info = App::RL::Common::decode_header($part); |
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65
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14
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50
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1942
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next unless App::RL::Common::info_is_valid($info); |
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66
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my $str = join( " ", |
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67
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$info->{chr}, $info->{start}, $info->{end}, |
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68
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14
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422
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"fill_color=" . $colors[$color_idx] ); |
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69
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14
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271
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push @lines, $str; |
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70
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} |
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71
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72
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# rotate color |
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73
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6
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11
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$color_idx++; |
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74
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6
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50
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27
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$color_idx = 0 if $color_idx > 11; |
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75
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} |
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76
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else { |
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77
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6
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17
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for ( my $i = 0; $i <= $#parts; $i++ ) { |
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78
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14
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49
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PAIR: for ( my $j = $i + 1; $j <= $#parts; $j++ ) { |
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79
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10
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16
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my @fields; |
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80
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10
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17
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for ( $i, $j ) { |
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81
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20
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248
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my $info |
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82
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= App::RL::Common::decode_header( $parts[$_] ); |
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83
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next PAIR |
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84
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20
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50
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2485
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unless App::RL::Common::info_is_valid($info); |
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85
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86
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push @fields, |
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87
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( |
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88
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$info->{chr}, |
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89
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$info->{strand} eq "+" |
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90
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? ( $info->{start}, $info->{end} ) |
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91
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: ( $info->{end}, $info->{start} ) |
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92
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20
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100
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506
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); |
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93
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} |
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94
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10
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214
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my $str = join( " ", @fields ); |
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95
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10
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48
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push @lines, $str; |
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96
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} |
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97
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} |
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98
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} |
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99
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} |
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100
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} |
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101
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2
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15
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@lines = List::MoreUtils::PP::uniq(@lines); |
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102
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103
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#----------------------------# |
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104
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# Output |
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105
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#----------------------------# |
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106
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2
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83
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my $out_fh; |
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107
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2
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50
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8
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if ( lc( $opt->{outfile} ) eq "stdout" ) { |
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108
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2
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7
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$out_fh = \*STDOUT; |
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109
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} |
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110
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else { |
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111
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0
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0
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open $out_fh, ">", $opt->{outfile}; |
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112
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} |
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113
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114
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2
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6
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print {$out_fh} "$_\n" for @lines; |
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24
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273
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115
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116
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2
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25
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close $out_fh; |
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117
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} |
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118
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119
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1; |