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1
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package Microarray::GEO::SOFT::GSM;
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2
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3
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# parse SOFT file
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4
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# get the GSM part, only the first record of GSM from the current position to read
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6
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1
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1
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2475
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use List::Vectorize qw(!table);
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1
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1327691
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1
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670
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7
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1
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1
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10
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use Carp;
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1
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1
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1
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65
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8
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1
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1
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6
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use strict;
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1
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2
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1
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40
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9
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10
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1
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1
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5
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use base "Microarray::GEO::SOFT";
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1
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2
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1
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745
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11
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12
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1;
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13
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14
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15
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sub new {
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16
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17
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my $invocant = shift;
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18
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my $class = ref($invocant) || $invocant;
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19
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my $self = { "file" => "",
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20
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"verbose" => 1,
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21
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"sample_value_column" => "VALUE",
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22
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@_ };
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bless($self, $class);
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25
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return $self;
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26
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27
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}
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28
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29
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sub parse {
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30
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31
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my $self = shift;
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32
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33
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my $fh;
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34
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if(! List::Vectorize::is_glob_ref($self->{file})) {
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35
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36
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open F, $self->{file} or croak "cannot open $self->{file}.\n";
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37
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$fh = \*F;
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38
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}
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else {
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40
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$fh = $self->{file};
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41
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}
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42
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43
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$self->_parse_sample($fh);
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45
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return 1;
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46
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}
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48
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sub _parse_sample {
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49
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50
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my $self = shift;
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51
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52
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my $fh = shift;
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53
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54
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Microarray::GEO::SOFT::_set_fh($self->{verbose});
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55
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56
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my $accession;
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57
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my $title;
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58
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my $platform;
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59
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my $table_colnames = [];
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60
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my $table_rownames = [];
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61
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my $table_matrix = [];
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62
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63
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while(my $line = <$fh>) {
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64
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65
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chomp $line;
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66
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if($line =~/^!Sample_geo_accession = (GSM\d+)$/) {
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67
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$accession = $1;
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68
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}
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69
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70
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elsif($line =~/^!Sample_title = (.*?)$/) {
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71
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$title = $1;
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72
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}
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73
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74
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elsif($line =~/^!Sample_platform_id = (GPL\d+)$/) {
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75
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$platform = $1;
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76
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}
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77
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78
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elsif($line =~/^!sample_table_begin$/) {
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79
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80
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$line = <$fh>;
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81
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chomp $line;
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82
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83
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@$table_colnames = split "\t", $line, -1;
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84
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shift(@$table_colnames);
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85
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86
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my $value_index = -1;
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87
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for(my $i = 0; $i < len($table_colnames); $i ++) {
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88
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if($table_colnames->[$i] eq $self->{sample_value_column}) {
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89
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$value_index = $i;
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90
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last;
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91
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}
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92
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}
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93
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94
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if($value_index == -1) {
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95
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croak "ERROR: Cannot find sample value column ($self->{sample_value_column}).";
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96
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}
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97
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98
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while($line = <$fh>) {
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99
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100
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if($line =~/^!sample_table_end$/) {
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101
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last;
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102
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}
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103
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104
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chomp $line;
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105
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my @tmp = split "\t", $line, -1;
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106
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107
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my $uid = shift(@tmp);
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108
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109
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push(@$table_rownames, $uid);
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110
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# one column matrix
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111
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push(@$table_matrix, [$tmp[$value_index]]);
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112
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}
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113
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114
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115
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}
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116
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117
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if($line =~/^!sample_table_end$/) {
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118
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last;
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119
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}
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120
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121
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}
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122
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123
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my $n_row = len($table_rownames);
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124
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my $n_col = len($table_colnames);
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125
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126
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print "Sample info:\n";
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127
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print " Accession: $accession\n";
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128
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print " Platform: $platform\n";
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129
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print " Title: $title\n";
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130
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print " Rows: $n_row\n";
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131
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print " Columns: $n_col\n";
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132
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print "\n";
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133
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134
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#my $table_rownames_sorted = sort_array($table_rownames, sub {$_[0] cmp $_[1]});
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135
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#my $table_rownames_sorted_index = order($table_rownames, sub {$_[0] cmp $_[1]});
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136
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#my $table_matrix_sorted = subset($table_matrix, $table_rownames_sorted_index);
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137
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138
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$self->set_meta( accession => $accession,
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139
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title => $title,
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140
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platform => $platform );
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141
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$self->set_table( rownames => $table_rownames,
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142
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colnames => $table_colnames,
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143
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matrix => $table_matrix );
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144
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145
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Microarray::GEO::SOFT::_set_to_std_fh();
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146
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147
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return $self;
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148
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149
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}
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150
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151
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152
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__END__
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