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package MW::ssNA; |
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use 5.006; |
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use strict; |
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require Exporter; |
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our @ISA = qw(Exporter); |
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our @EXPORT = qw(ssNA); |
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=head1 NAME |
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MW::ssNA - Perl extension to calculate molecular weight of ssDNA or ssRNA. |
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=head1 VERSION |
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Version 1.00 |
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=cut |
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our $VERSION = '1.00'; |
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=head1 SYNOPSIS |
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use MW::ssNA; |
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my @foo = ssNA("input_filename","option"); |
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# option : 'd'-DNA, 'r'-RNA, 'cd'-total count of ATGC in DNA, 'cr'-total count of AUGC in RNA |
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foreach(@foo) { print;} |
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=cut |
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sub ssNA { |
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my $input=$_[0]; |
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my $choice=$_[1]; |
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my $flag11=0; |
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my ($flag,$count,$A,$T,$G,$C,$ssdna); |
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my ($x,$a1,$a2,$a3,$a4,$tu); |
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if ($choice eq 'd') |
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{ |
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$a1=313.2; |
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$a2=304.2; |
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$a3=329.2; |
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$a4=289.2; |
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$tu='T'; |
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} |
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elsif ($choice eq 'r') |
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{ |
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$a1=329.2; |
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$a2=306.2; |
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$a3=345.2; |
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$a4=305.2; |
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$tu='U'; |
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} |
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elsif ($choice eq 'cd') {$flag11=1;$tu='T';} |
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elsif ($choice eq 'cr') {$flag11=1;$tu='U';} |
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else {print "Invalid option!";exit;} |
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open(ID,"$input") or die "Could not open $input: $!";; |
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$flag=$count=0; |
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while() |
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{ |
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chomp $_; |
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if(eof) |
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{ |
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$A+=()=$_=~/A/g; $T+=()=$_=~/$tu/g; $G+=()=$_=~/G/g; $C+=()=$_=~/C/g; |
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$count++; |
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$flag=1; |
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} |
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if ($_=~/^>/ || $flag == 1) |
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{ |
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if($count!=0) |
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{ |
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$ssdna=($A*$a1)+($T*$a2)+($G*$a3)+($C*$a4); |
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if ($flag11 == 1) { print "$x\t A=$A\t$tu=$T\tC=$C\tG=$G\n"; } |
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else {print "$x\tMW : $ssdna\n";} |
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$A=$T=$G=$C=''; |
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} |
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$x=$_; |
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next; |
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} |
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$A+=()=$_=~/A/g; $T+=()=$_=~/$tu/g; $G+=()=$_=~/G/g; $C+=()=$_=~/C/g; |
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$count++; |
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} |
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} |
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=head1 AUTHOR |
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SHANKAR M, C<< >> |
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=head1 BUGS |
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Please report any bugs or feature requests to C |
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=head1 ACKNOWLEDGEMENTS |
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Saravanan S E and Sabarinathan Radhakrishnan, for all their valuable thoughts and care. |
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=head1 LICENSE AND COPYRIGHT |
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Copyright 2011 Shankar M. |
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102
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This program is free software; you can redistribute it and/or modify it under the same terms as Perl itself. |
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104
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105
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=cut |
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1; # End of MW::ssNA |