| line | stmt | bran | cond | sub | pod | time | code | 
| 1 |  |  |  |  |  |  | # POD documentation - main docs before the code | 
| 2 |  |  |  |  |  |  |  | 
| 3 |  |  |  |  |  |  | =head1 NAME | 
| 4 |  |  |  |  |  |  |  | 
| 5 |  |  |  |  |  |  | GenOO::Data::File::BED - Object implementing methods for accessing bed formatted files (http://genome.ucsc.edu/FAQ/FAQformat#format1) | 
| 6 |  |  |  |  |  |  |  | 
| 7 |  |  |  |  |  |  | =head1 SYNOPSIS | 
| 8 |  |  |  |  |  |  |  | 
| 9 |  |  |  |  |  |  | # Object that manages a bed file. | 
| 10 |  |  |  |  |  |  |  | 
| 11 |  |  |  |  |  |  | # To initialize | 
| 12 |  |  |  |  |  |  | my $bed_file = GenOO::Data::File::BED->new({ | 
| 13 |  |  |  |  |  |  | FILE            => undef, | 
| 14 |  |  |  |  |  |  | EXTRA_INFO      => undef, | 
| 15 |  |  |  |  |  |  | }); | 
| 16 |  |  |  |  |  |  |  | 
| 17 |  |  |  |  |  |  | =head1 DESCRIPTION | 
| 18 |  |  |  |  |  |  |  | 
| 19 |  |  |  |  |  |  | This object offers methods to read a bed file line by line. | 
| 20 |  |  |  |  |  |  |  | 
| 21 |  |  |  |  |  |  | =head1 EXAMPLES | 
| 22 |  |  |  |  |  |  |  | 
| 23 |  |  |  |  |  |  | # Create object | 
| 24 |  |  |  |  |  |  | my $bed_file = GenOO::Data::File::BED->new({ | 
| 25 |  |  |  |  |  |  | FILE => 't/sample_data/sample.bed.gz' | 
| 26 |  |  |  |  |  |  | }); | 
| 27 |  |  |  |  |  |  |  | 
| 28 |  |  |  |  |  |  | # Read one record at a time | 
| 29 |  |  |  |  |  |  | my $record = $bed_file->next_record(); | 
| 30 |  |  |  |  |  |  |  | 
| 31 |  |  |  |  |  |  | =cut | 
| 32 |  |  |  |  |  |  |  | 
| 33 |  |  |  |  |  |  | # Let the code begin... | 
| 34 |  |  |  |  |  |  |  | 
| 35 |  |  |  |  |  |  | package GenOO::Data::File::BED; | 
| 36 |  |  |  |  |  |  | $GenOO::Data::File::BED::VERSION = '1.5.2'; | 
| 37 |  |  |  |  |  |  |  | 
| 38 |  |  |  |  |  |  | ####################################################################### | 
| 39 |  |  |  |  |  |  | #######################   Load External modules   ##################### | 
| 40 |  |  |  |  |  |  | ####################################################################### | 
| 41 | 1 |  |  | 1 |  | 5819 | use Modern::Perl; | 
|  | 1 |  |  |  |  | 4 |  | 
|  | 1 |  |  |  |  | 32 |  | 
| 42 | 1 |  |  | 1 |  | 369 | use autodie; | 
|  | 1 |  |  |  |  | 2 |  | 
|  | 1 |  |  |  |  | 23 |  | 
| 43 | 1 |  |  | 1 |  | 6441 | use Moose; | 
|  | 1 |  |  |  |  | 6 |  | 
|  | 1 |  |  |  |  | 28 |  | 
| 44 | 1 |  |  | 1 |  | 11700 | use namespace::autoclean; | 
|  | 1 |  |  |  |  | 2 |  | 
|  | 1 |  |  |  |  | 28 |  | 
| 45 |  |  |  |  |  |  |  | 
| 46 |  |  |  |  |  |  |  | 
| 47 |  |  |  |  |  |  | ####################################################################### | 
| 48 |  |  |  |  |  |  | #########################   Load GenOO modules   ###################### | 
| 49 |  |  |  |  |  |  | ####################################################################### | 
| 50 | 1 |  |  | 1 |  | 873 | use GenOO::Data::File::BED::Record; | 
|  | 1 |  |  |  |  | 3 |  | 
|  | 1 |  |  |  |  | 1917 |  | 
| 51 |  |  |  |  |  |  |  | 
| 52 |  |  |  |  |  |  |  | 
| 53 |  |  |  |  |  |  | ####################################################################### | 
| 54 |  |  |  |  |  |  | #######################   Interface attributes   ###################### | 
| 55 |  |  |  |  |  |  | ####################################################################### | 
| 56 |  |  |  |  |  |  | has 'file' => ( | 
| 57 |  |  |  |  |  |  | isa      => 'Maybe[Str]', | 
| 58 |  |  |  |  |  |  | is       => 'rw', | 
| 59 |  |  |  |  |  |  | required => 1 | 
| 60 |  |  |  |  |  |  | ); | 
| 61 |  |  |  |  |  |  |  | 
| 62 |  |  |  |  |  |  | has 'redirect_score_to_copy_number' => ( | 
| 63 |  |  |  |  |  |  | traits  => ['Bool'], | 
| 64 |  |  |  |  |  |  | is      => 'rw', | 
| 65 |  |  |  |  |  |  | isa     => 'Bool', | 
| 66 |  |  |  |  |  |  | default => 0, | 
| 67 |  |  |  |  |  |  | lazy    => 1 | 
| 68 |  |  |  |  |  |  | ); | 
| 69 |  |  |  |  |  |  |  | 
| 70 |  |  |  |  |  |  |  | 
| 71 |  |  |  |  |  |  | ####################################################################### | 
| 72 |  |  |  |  |  |  | ########################   Private attributes   ####################### | 
| 73 |  |  |  |  |  |  | ####################################################################### | 
| 74 |  |  |  |  |  |  | has '_filehandle' => ( | 
| 75 |  |  |  |  |  |  | is        => 'ro', | 
| 76 |  |  |  |  |  |  | builder   => '_open_filehandle', | 
| 77 |  |  |  |  |  |  | init_arg  => undef, | 
| 78 |  |  |  |  |  |  | lazy      => 1, | 
| 79 |  |  |  |  |  |  | ); | 
| 80 |  |  |  |  |  |  |  | 
| 81 |  |  |  |  |  |  |  | 
| 82 |  |  |  |  |  |  | ####################################################################### | 
| 83 |  |  |  |  |  |  | ##############################   BUILD   ############################## | 
| 84 |  |  |  |  |  |  | ####################################################################### | 
| 85 |  |  |  |  |  |  | sub BUILD { | 
| 86 | 20 |  |  | 20 | 0 | 40 | my $self = shift; | 
| 87 |  |  |  |  |  |  |  | 
| 88 | 20 |  |  |  |  | 81 | $self->init_header; | 
| 89 | 20 |  |  |  |  | 164 | $self->init_records_cache; | 
| 90 | 20 |  |  |  |  | 145 | $self->init_records_read_count; | 
| 91 |  |  |  |  |  |  |  | 
| 92 | 20 |  |  |  |  | 141 | $self->parse_header_section; | 
| 93 |  |  |  |  |  |  | } | 
| 94 |  |  |  |  |  |  |  | 
| 95 |  |  |  |  |  |  |  | 
| 96 |  |  |  |  |  |  | ####################################################################### | 
| 97 |  |  |  |  |  |  | ########################   Interface Methods   ######################## | 
| 98 |  |  |  |  |  |  | ####################################################################### | 
| 99 |  |  |  |  |  |  | sub records_read_count { | 
| 100 | 5 |  |  | 5 | 0 | 3797 | my ($self) = @_; | 
| 101 | 5 |  |  |  |  | 68 | return $self->{RECORDS_READ_COUNT}; | 
| 102 |  |  |  |  |  |  | } | 
| 103 |  |  |  |  |  |  |  | 
| 104 |  |  |  |  |  |  | sub next_record { | 
| 105 | 54 |  |  | 54 | 0 | 3930 | my ($self) = @_; | 
| 106 |  |  |  |  |  |  |  | 
| 107 | 54 |  |  |  |  | 137 | my $record; | 
| 108 | 54 | 100 |  |  |  | 147 | if ($self->record_cache_not_empty) { | 
| 109 | 9 |  |  |  |  | 43 | $record = $self->next_record_from_cache; | 
| 110 |  |  |  |  |  |  | } | 
| 111 |  |  |  |  |  |  | else { | 
| 112 | 45 |  |  |  |  | 104 | $record = $self->next_record_from_file; | 
| 113 |  |  |  |  |  |  | } | 
| 114 |  |  |  |  |  |  |  | 
| 115 | 54 | 100 |  |  |  | 143 | if (defined $record) { | 
| 116 | 49 |  |  |  |  | 198 | $self->increment_records_read_count; | 
| 117 |  |  |  |  |  |  | } | 
| 118 | 54 |  |  |  |  | 445 | return $record; | 
| 119 |  |  |  |  |  |  | } | 
| 120 |  |  |  |  |  |  |  | 
| 121 |  |  |  |  |  |  |  | 
| 122 |  |  |  |  |  |  | ####################################################################### | 
| 123 |  |  |  |  |  |  | #######################   Private Methods  ############################ | 
| 124 |  |  |  |  |  |  | ####################################################################### | 
| 125 |  |  |  |  |  |  | sub set_eof_reached { | 
| 126 | 5 |  |  | 5 | 0 | 22 | my ($self) = @_; | 
| 127 | 5 |  |  |  |  | 47 | $self->{EOF} = 1; | 
| 128 |  |  |  |  |  |  | } | 
| 129 |  |  |  |  |  |  |  | 
| 130 |  |  |  |  |  |  | sub header { | 
| 131 | 1 |  |  | 1 | 0 | 3879 | my ($self) = @_; | 
| 132 | 1 |  |  |  |  | 30 | return $self->{HEADER}; | 
| 133 |  |  |  |  |  |  | } | 
| 134 |  |  |  |  |  |  |  | 
| 135 |  |  |  |  |  |  | sub records_cache { | 
| 136 | 61 |  |  | 61 | 0 | 3927 | my ($self) = @_; | 
| 137 | 61 |  |  |  |  | 398 | return $self->{RECORDS_CACHE}; | 
| 138 |  |  |  |  |  |  | } | 
| 139 |  |  |  |  |  |  |  | 
| 140 |  |  |  |  |  |  | sub init_header { | 
| 141 | 20 |  |  | 20 | 0 | 44 | my ($self) = @_; | 
| 142 | 20 |  |  |  |  | 70 | $self->{HEADER} = {}; | 
| 143 |  |  |  |  |  |  | } | 
| 144 |  |  |  |  |  |  |  | 
| 145 |  |  |  |  |  |  | sub init_records_cache { | 
| 146 | 20 |  |  | 20 | 0 | 55 | my ($self) = @_; | 
| 147 | 20 |  |  |  |  | 84 | $self->{RECORDS_CACHE} = []; | 
| 148 |  |  |  |  |  |  | } | 
| 149 |  |  |  |  |  |  |  | 
| 150 |  |  |  |  |  |  | sub init_records_read_count { | 
| 151 | 20 |  |  | 20 | 0 | 60 | my ($self) = @_; | 
| 152 | 20 |  |  |  |  | 61 | $self->{RECORDS_READ_COUNT} = 0; | 
| 153 |  |  |  |  |  |  | } | 
| 154 |  |  |  |  |  |  |  | 
| 155 |  |  |  |  |  |  | sub increment_records_read_count { | 
| 156 | 50 |  |  | 50 | 0 | 3871 | my ($self) = @_; | 
| 157 | 50 |  |  |  |  | 117 | $self->{RECORDS_READ_COUNT}++; | 
| 158 |  |  |  |  |  |  | } | 
| 159 |  |  |  |  |  |  |  | 
| 160 |  |  |  |  |  |  | sub parse_header_section { | 
| 161 | 20 |  |  | 20 | 0 | 69 | my ($self) = @_; | 
| 162 |  |  |  |  |  |  |  | 
| 163 | 20 |  |  |  |  | 696 | my $filehandle = $self->_filehandle; | 
| 164 | 20 |  |  |  |  | 892 | while (my $line = $filehandle->getline) { | 
| 165 | 80 | 100 |  |  |  | 4262 | if ($self->line_looks_like_header($line)) { | 
|  |  | 50 |  |  |  |  |  | 
| 166 | 60 |  |  |  |  | 443 | $self->recognize_and_store_header_line($line); | 
| 167 |  |  |  |  |  |  | } | 
| 168 |  |  |  |  |  |  | elsif ($self->line_looks_like_record($line)) { | 
| 169 |  |  |  |  |  |  | # the while loop will read one line after header. Usually, this is the first record and unfortunately in zipped files we cannot go back | 
| 170 | 20 |  |  |  |  | 173 | my $record = $self->parse_record_line($line); | 
| 171 | 20 |  |  |  |  | 197 | $self->add_to_records_cache($record); | 
| 172 | 20 |  |  |  |  | 717 | return; | 
| 173 |  |  |  |  |  |  | } | 
| 174 |  |  |  |  |  |  | else { | 
| 175 | 0 |  |  |  |  | 0 | return; | 
| 176 |  |  |  |  |  |  | } | 
| 177 |  |  |  |  |  |  | } | 
| 178 |  |  |  |  |  |  | } | 
| 179 |  |  |  |  |  |  |  | 
| 180 |  |  |  |  |  |  | # TODO fix to store "browser" and "track" lines | 
| 181 |  |  |  |  |  |  | sub recognize_and_store_header_line { | 
| 182 | 60 |  |  | 60 | 0 | 1293 | my ($self, $line) = @_; | 
| 183 |  |  |  |  |  |  | # 	if ($self->line_looks_like_version($line)) { | 
| 184 |  |  |  |  |  |  | # 		$self->parse_and_store_version_line($line); | 
| 185 |  |  |  |  |  |  | # 	} | 
| 186 |  |  |  |  |  |  | # 	else { | 
| 187 |  |  |  |  |  |  | # 		$self->parse_and_store_header_line($line); | 
| 188 |  |  |  |  |  |  | # 	} | 
| 189 |  |  |  |  |  |  | } | 
| 190 |  |  |  |  |  |  |  | 
| 191 |  |  |  |  |  |  | sub add_to_records_cache { | 
| 192 | 20 |  |  | 20 | 0 | 56 | my ($self, $record) = @_; | 
| 193 | 20 |  |  |  |  | 42 | push @{$self->{RECORDS_CACHE}}, $record, | 
|  | 20 |  |  |  |  | 185 |  | 
| 194 |  |  |  |  |  |  | } | 
| 195 |  |  |  |  |  |  |  | 
| 196 |  |  |  |  |  |  | sub next_record_from_file { | 
| 197 | 46 |  |  | 46 | 0 | 3792 | my ($self) = @_; | 
| 198 |  |  |  |  |  |  |  | 
| 199 | 46 |  |  |  |  | 1357 | while (my $line = $self->_filehandle->getline) { | 
| 200 | 41 | 50 |  |  |  | 1074 | if ($self->line_looks_like_record($line)) { | 
| 201 | 41 |  |  |  |  | 100 | return $self->parse_record_line($line); | 
| 202 |  |  |  |  |  |  | } | 
| 203 |  |  |  |  |  |  | else { | 
| 204 | 0 | 0 |  |  |  | 0 | if ($self->line_looks_like_header($line)) { | 
| 205 | 0 |  |  |  |  | 0 | die "Record was expected but line looks like a header - the header should have been parsed already. $line\n"; | 
| 206 |  |  |  |  |  |  | } | 
| 207 |  |  |  |  |  |  | else { | 
| 208 | 0 |  |  |  |  | 0 | warn "Record was expected but line looks different. $line\n"; | 
| 209 |  |  |  |  |  |  | } | 
| 210 |  |  |  |  |  |  | } | 
| 211 |  |  |  |  |  |  | } | 
| 212 |  |  |  |  |  |  |  | 
| 213 | 5 |  |  |  |  | 247 | $self->set_eof_reached; | 
| 214 | 5 |  |  |  |  | 13 | return undef; | 
| 215 |  |  |  |  |  |  | } | 
| 216 |  |  |  |  |  |  |  | 
| 217 |  |  |  |  |  |  | sub next_record_from_cache { | 
| 218 | 10 |  |  | 10 | 0 | 3940 | my ($self) = @_; | 
| 219 |  |  |  |  |  |  |  | 
| 220 | 10 |  |  |  |  | 30 | my $record = shift @{$self->{RECORDS_CACHE}}; | 
|  | 10 |  |  |  |  | 48 |  | 
| 221 | 10 | 50 |  |  |  | 35 | if (defined $record) { | 
| 222 | 10 |  |  |  |  | 61 | return $record; | 
| 223 |  |  |  |  |  |  | } | 
| 224 |  |  |  |  |  |  | else { | 
| 225 | 0 |  |  |  |  | 0 | return undef; | 
| 226 |  |  |  |  |  |  | } | 
| 227 |  |  |  |  |  |  | } | 
| 228 |  |  |  |  |  |  |  | 
| 229 |  |  |  |  |  |  | sub parse_record_line { | 
| 230 | 62 |  |  | 62 | 0 | 4164 | my ($self, $line) = @_; | 
| 231 |  |  |  |  |  |  |  | 
| 232 | 62 |  |  |  |  | 216 | chomp $line; | 
| 233 | 62 |  |  |  |  | 797 | my ($chr,$start,$stop_1,$name,$score,$strand,$thick_start,$thick_stop,$rgb,$block_count,$block_sizes,$block_starts) = split(/\t/,$line); | 
| 234 |  |  |  |  |  |  |  | 
| 235 | 62 |  |  |  |  | 934 | my $data = { | 
| 236 |  |  |  |  |  |  | rname             => $chr, | 
| 237 |  |  |  |  |  |  | start             => $start, | 
| 238 |  |  |  |  |  |  | stop_1based       => $stop_1, | 
| 239 |  |  |  |  |  |  | name              => $name, | 
| 240 |  |  |  |  |  |  | score             => $score, | 
| 241 |  |  |  |  |  |  | strand_symbol     => $strand, | 
| 242 |  |  |  |  |  |  | }; | 
| 243 |  |  |  |  |  |  |  | 
| 244 | 62 | 100 |  |  |  | 2285 | ($data->{copy_number}       = $score) if $self->redirect_score_to_copy_number; | 
| 245 | 62 | 50 |  |  |  | 440 | ($data->{thick_start}       = $thick_start) if defined $thick_start; | 
| 246 | 62 | 50 |  |  |  | 383 | ($data->{thick_stop_1based} = $thick_stop) if defined $thick_stop; | 
| 247 | 62 | 50 |  |  |  | 450 | ($data->{rgb}               = $rgb) if defined $rgb; | 
| 248 | 62 | 100 |  |  |  | 234 | ($data->{block_count}       = $block_count) if defined $block_count; | 
| 249 | 62 | 100 |  |  |  | 196 | ($data->{block_sizes}       = [split(/,/,$block_sizes)]) if defined $block_sizes; | 
| 250 | 62 | 100 |  |  |  | 240 | ($data->{block_starts}      = [split(/,/,$block_starts)]) if defined $block_starts; | 
| 251 |  |  |  |  |  |  |  | 
| 252 | 62 |  |  |  |  | 2479 | return GenOO::Data::File::BED::Record->new($data); | 
| 253 |  |  |  |  |  |  | } | 
| 254 |  |  |  |  |  |  |  | 
| 255 |  |  |  |  |  |  | sub line_looks_like_comment { | 
| 256 | 1 |  |  | 1 | 0 | 3744 | my ($self, $line) = @_; | 
| 257 | 1 | 50 |  |  |  | 35 | return ($line =~ /^#/) ? 1 : 0; | 
| 258 |  |  |  |  |  |  | } | 
| 259 |  |  |  |  |  |  |  | 
| 260 |  |  |  |  |  |  | sub line_looks_like_header { | 
| 261 | 83 |  |  | 83 | 0 | 4037 | my ($self, $line) = @_; | 
| 262 | 83 | 100 |  |  |  | 1151 | return ($line =~ /^(track|browser)/) ? 1 : 0; | 
| 263 |  |  |  |  |  |  | } | 
| 264 |  |  |  |  |  |  |  | 
| 265 |  |  |  |  |  |  | sub line_looks_like_record { | 
| 266 | 65 |  |  | 65 | 0 | 3966 | my ($self, $line) = @_; | 
| 267 | 65 | 100 |  |  |  | 635 | return ($line !~ /^(#|track|browser)/) ? 1 : 0; | 
| 268 |  |  |  |  |  |  | } | 
| 269 |  |  |  |  |  |  |  | 
| 270 |  |  |  |  |  |  | sub record_cache_not_empty { | 
| 271 | 56 |  |  | 56 | 0 | 3985 | my ($self) = @_; | 
| 272 | 56 | 100 |  |  |  | 157 | return ($self->record_cache_size > 0) ? 1 : 0; | 
| 273 |  |  |  |  |  |  | } | 
| 274 |  |  |  |  |  |  |  | 
| 275 |  |  |  |  |  |  | sub record_cache_is_empty { | 
| 276 | 2 |  |  | 2 | 0 | 3986 | my ($self) = @_; | 
| 277 | 2 | 100 |  |  |  | 28 | return ($self->record_cache_size == 0) ? 1 : 0; | 
| 278 |  |  |  |  |  |  | } | 
| 279 |  |  |  |  |  |  |  | 
| 280 |  |  |  |  |  |  | sub record_cache_size { | 
| 281 | 60 |  |  | 60 | 0 | 3962 | my ($self) = @_; | 
| 282 | 60 |  |  |  |  | 86 | return scalar @{$self->records_cache}; | 
|  | 60 |  |  |  |  | 162 |  | 
| 283 |  |  |  |  |  |  | } | 
| 284 |  |  |  |  |  |  |  | 
| 285 |  |  |  |  |  |  | sub is_eof_reached { | 
| 286 | 2 |  |  | 2 | 0 | 3773 | my ($self) = @_; | 
| 287 | 2 |  |  |  |  | 32 | return $self->{EOF}; | 
| 288 |  |  |  |  |  |  | } | 
| 289 |  |  |  |  |  |  |  | 
| 290 |  |  |  |  |  |  |  | 
| 291 |  |  |  |  |  |  | ####################################################################### | 
| 292 |  |  |  |  |  |  | #########################   Private Methods   ######################### | 
| 293 |  |  |  |  |  |  | ####################################################################### | 
| 294 |  |  |  |  |  |  | sub _open_filehandle { | 
| 295 | 20 |  |  | 20 |  | 50 | my ($self) = @_; | 
| 296 |  |  |  |  |  |  |  | 
| 297 | 20 |  |  |  |  | 50 | my $read_mode; | 
| 298 |  |  |  |  |  |  | my $HANDLE; | 
| 299 | 20 | 50 |  |  |  | 575 | if (!defined $self->file) { | 
|  |  | 50 |  |  |  |  |  | 
| 300 | 0 |  |  |  |  | 0 | open ($HANDLE, '<-', $self->file); | 
| 301 |  |  |  |  |  |  | } | 
| 302 |  |  |  |  |  |  | elsif ($self->file =~ /\.gz$/) { | 
| 303 | 20 | 50 |  |  |  | 552 | die 'Cannot open file ' . $self->file . "\n" if ! -e $self->file; | 
| 304 | 20 |  |  |  |  | 628 | open($HANDLE, 'gzip -dc ' . $self->file . ' |'); | 
| 305 |  |  |  |  |  |  | } | 
| 306 |  |  |  |  |  |  | else { | 
| 307 | 0 |  |  |  |  | 0 | open ($HANDLE, '<', $self->file); | 
| 308 |  |  |  |  |  |  | } | 
| 309 |  |  |  |  |  |  |  | 
| 310 | 20 |  |  |  |  | 93267 | return $HANDLE; | 
| 311 |  |  |  |  |  |  | } | 
| 312 |  |  |  |  |  |  |  | 
| 313 |  |  |  |  |  |  |  | 
| 314 |  |  |  |  |  |  | ####################################################################### | 
| 315 |  |  |  |  |  |  | ############################   Finalize   ############################# | 
| 316 |  |  |  |  |  |  | ####################################################################### | 
| 317 |  |  |  |  |  |  | __PACKAGE__->meta->make_immutable; | 
| 318 |  |  |  |  |  |  |  | 
| 319 |  |  |  |  |  |  | 1; |