line |
stmt |
bran |
cond |
sub |
pod |
time |
code |
1
|
|
|
|
|
|
|
# POD documentation - main docs before the code |
2
|
|
|
|
|
|
|
|
3
|
|
|
|
|
|
|
=head1 NAME |
4
|
|
|
|
|
|
|
|
5
|
|
|
|
|
|
|
GenOO::Data::File::GFF - Object implementing methods for accessing GFF formatted files (http://www.sanger.ac.uk/resources/software/gff/spec.html) |
6
|
|
|
|
|
|
|
|
7
|
|
|
|
|
|
|
=head1 SYNOPSIS |
8
|
|
|
|
|
|
|
|
9
|
|
|
|
|
|
|
# Object that manages a gff file. |
10
|
|
|
|
|
|
|
|
11
|
|
|
|
|
|
|
# To initialize |
12
|
|
|
|
|
|
|
my $gff_file = GenOO::Data::File::GFF->new( |
13
|
|
|
|
|
|
|
file => undef, |
14
|
|
|
|
|
|
|
); |
15
|
|
|
|
|
|
|
|
16
|
|
|
|
|
|
|
|
17
|
|
|
|
|
|
|
=head1 DESCRIPTION |
18
|
|
|
|
|
|
|
|
19
|
|
|
|
|
|
|
This object offers methods to read a gff file line by line. |
20
|
|
|
|
|
|
|
|
21
|
|
|
|
|
|
|
=head1 EXAMPLES |
22
|
|
|
|
|
|
|
|
23
|
|
|
|
|
|
|
# Create object |
24
|
|
|
|
|
|
|
my $gff_file = GenOO::Data::File::GFF->new( |
25
|
|
|
|
|
|
|
file => 't/sample_data/sample.gff.gz' |
26
|
|
|
|
|
|
|
); |
27
|
|
|
|
|
|
|
|
28
|
|
|
|
|
|
|
# Read one record at a time |
29
|
|
|
|
|
|
|
my $record = $gff_file->next_record(); |
30
|
|
|
|
|
|
|
|
31
|
|
|
|
|
|
|
=cut |
32
|
|
|
|
|
|
|
|
33
|
|
|
|
|
|
|
# Let the code begin... |
34
|
|
|
|
|
|
|
|
35
|
|
|
|
|
|
|
|
36
|
|
|
|
|
|
|
package GenOO::Data::File::GFF; |
37
|
|
|
|
|
|
|
$GenOO::Data::File::GFF::VERSION = '1.5.1'; |
38
|
|
|
|
|
|
|
|
39
|
|
|
|
|
|
|
####################################################################### |
40
|
|
|
|
|
|
|
####################### Load External modules ##################### |
41
|
|
|
|
|
|
|
####################################################################### |
42
|
1
|
|
|
1
|
|
4
|
use Modern::Perl; |
|
1
|
|
|
|
|
2
|
|
|
1
|
|
|
|
|
7
|
|
43
|
1
|
|
|
1
|
|
130
|
use autodie; |
|
1
|
|
|
|
|
1
|
|
|
1
|
|
|
|
|
7
|
|
44
|
1
|
|
|
1
|
|
2898
|
use Moose; |
|
1
|
|
|
|
|
1
|
|
|
1
|
|
|
|
|
6
|
|
45
|
1
|
|
|
1
|
|
4804
|
use namespace::autoclean; |
|
1
|
|
|
|
|
2
|
|
|
1
|
|
|
|
|
8
|
|
46
|
|
|
|
|
|
|
|
47
|
|
|
|
|
|
|
|
48
|
|
|
|
|
|
|
####################################################################### |
49
|
|
|
|
|
|
|
######################### Load GenOO modules ###################### |
50
|
|
|
|
|
|
|
####################################################################### |
51
|
1
|
|
|
1
|
|
537
|
use GenOO::Data::File::GFF::Record; |
|
1
|
|
|
|
|
2
|
|
|
1
|
|
|
|
|
1203
|
|
52
|
|
|
|
|
|
|
|
53
|
|
|
|
|
|
|
|
54
|
|
|
|
|
|
|
####################################################################### |
55
|
|
|
|
|
|
|
####################### Interface attributes ###################### |
56
|
|
|
|
|
|
|
####################################################################### |
57
|
|
|
|
|
|
|
has 'file' => ( |
58
|
|
|
|
|
|
|
isa => 'Maybe[Str]', |
59
|
|
|
|
|
|
|
is => 'rw', |
60
|
|
|
|
|
|
|
required => 1 |
61
|
|
|
|
|
|
|
); |
62
|
|
|
|
|
|
|
|
63
|
|
|
|
|
|
|
has 'records_read_count' => ( |
64
|
|
|
|
|
|
|
traits => ['Counter'], |
65
|
|
|
|
|
|
|
is => 'ro', |
66
|
|
|
|
|
|
|
isa => 'Num', |
67
|
|
|
|
|
|
|
default => 0, |
68
|
|
|
|
|
|
|
handles => { |
69
|
|
|
|
|
|
|
_inc_records_read_count => 'inc', |
70
|
|
|
|
|
|
|
_reset_records_read_count => 'reset', |
71
|
|
|
|
|
|
|
}, |
72
|
|
|
|
|
|
|
); |
73
|
|
|
|
|
|
|
|
74
|
|
|
|
|
|
|
|
75
|
|
|
|
|
|
|
####################################################################### |
76
|
|
|
|
|
|
|
######################## Private attributes ####################### |
77
|
|
|
|
|
|
|
####################################################################### |
78
|
|
|
|
|
|
|
has '_filehandle' => ( |
79
|
|
|
|
|
|
|
is => 'ro', |
80
|
|
|
|
|
|
|
builder => '_open_filehandle', |
81
|
|
|
|
|
|
|
init_arg => undef, |
82
|
|
|
|
|
|
|
lazy => 1, |
83
|
|
|
|
|
|
|
); |
84
|
|
|
|
|
|
|
|
85
|
|
|
|
|
|
|
has '_eof_reached' => ( |
86
|
|
|
|
|
|
|
is => 'rw', |
87
|
|
|
|
|
|
|
default => 0, |
88
|
|
|
|
|
|
|
init_arg => undef, |
89
|
|
|
|
|
|
|
lazy => 1, |
90
|
|
|
|
|
|
|
); |
91
|
|
|
|
|
|
|
|
92
|
|
|
|
|
|
|
has '_header' => ( |
93
|
|
|
|
|
|
|
is => 'ro', |
94
|
|
|
|
|
|
|
default => sub {{}}, |
95
|
|
|
|
|
|
|
init_arg => undef, |
96
|
|
|
|
|
|
|
lazy => 1, |
97
|
|
|
|
|
|
|
); |
98
|
|
|
|
|
|
|
|
99
|
|
|
|
|
|
|
has '_cached_records' => ( |
100
|
|
|
|
|
|
|
traits => ['Array'], |
101
|
|
|
|
|
|
|
is => 'ro', |
102
|
|
|
|
|
|
|
isa => 'ArrayRef[GenOO::Data::File::GFF::Record]', |
103
|
|
|
|
|
|
|
default => sub { [] }, |
104
|
|
|
|
|
|
|
handles => { |
105
|
|
|
|
|
|
|
_all_cached_records => 'elements', |
106
|
|
|
|
|
|
|
_add_record_in_cache => 'push', |
107
|
|
|
|
|
|
|
_shift_cached_record => 'shift', |
108
|
|
|
|
|
|
|
_has_cached_records => 'count', |
109
|
|
|
|
|
|
|
_has_no_cached_records => 'is_empty', |
110
|
|
|
|
|
|
|
}, |
111
|
|
|
|
|
|
|
); |
112
|
|
|
|
|
|
|
|
113
|
|
|
|
|
|
|
|
114
|
|
|
|
|
|
|
####################################################################### |
115
|
|
|
|
|
|
|
############################### BUILD ############################# |
116
|
|
|
|
|
|
|
####################################################################### |
117
|
|
|
|
|
|
|
sub BUILD { |
118
|
31
|
|
|
31
|
0
|
68
|
my $self = shift; |
119
|
|
|
|
|
|
|
|
120
|
31
|
|
|
|
|
162
|
$self->_parse_header_section; |
121
|
|
|
|
|
|
|
} |
122
|
|
|
|
|
|
|
|
123
|
|
|
|
|
|
|
####################################################################### |
124
|
|
|
|
|
|
|
######################## Interface Methods ######################## |
125
|
|
|
|
|
|
|
####################################################################### |
126
|
|
|
|
|
|
|
sub next_record { |
127
|
10950
|
|
|
10950
|
0
|
15282
|
my ($self) = @_; |
128
|
|
|
|
|
|
|
|
129
|
10950
|
|
|
|
|
8192
|
my $record; |
130
|
10950
|
100
|
|
|
|
389962
|
if ($self->_has_cached_records) { |
131
|
20
|
|
|
|
|
1028
|
$record = $self->_shift_cached_record; |
132
|
|
|
|
|
|
|
} |
133
|
|
|
|
|
|
|
else { |
134
|
10930
|
|
|
|
|
22312
|
$record = $self->_next_record_from_file; |
135
|
|
|
|
|
|
|
} |
136
|
|
|
|
|
|
|
|
137
|
10950
|
100
|
|
|
|
24851
|
if (defined $record) { |
138
|
10933
|
|
|
|
|
408818
|
$self->_inc_records_read_count; |
139
|
|
|
|
|
|
|
} |
140
|
10950
|
|
|
|
|
32266
|
return $record; |
141
|
|
|
|
|
|
|
} |
142
|
|
|
|
|
|
|
|
143
|
|
|
|
|
|
|
sub version { |
144
|
1
|
|
|
1
|
0
|
2350
|
my ($self) = @_; |
145
|
1
|
|
|
|
|
63
|
return $self->_header->{VERSION}; |
146
|
|
|
|
|
|
|
} |
147
|
|
|
|
|
|
|
|
148
|
|
|
|
|
|
|
|
149
|
|
|
|
|
|
|
####################################################################### |
150
|
|
|
|
|
|
|
######################### Private Methods ######################### |
151
|
|
|
|
|
|
|
####################################################################### |
152
|
|
|
|
|
|
|
sub _parse_header_section { |
153
|
31
|
|
|
31
|
|
54
|
my ($self) = @_; |
154
|
|
|
|
|
|
|
|
155
|
31
|
|
|
|
|
1011
|
my $filehandle = $self->_filehandle; |
156
|
31
|
|
|
|
|
1465
|
while (my $line = $filehandle->getline) { |
157
|
143
|
100
|
|
|
|
22735
|
if ($self->_line_looks_like_header($line)) { |
|
|
100
|
|
|
|
|
|
158
|
32
|
|
|
|
|
211
|
$self->_recognize_and_store_header_line($line); |
159
|
|
|
|
|
|
|
} |
160
|
|
|
|
|
|
|
elsif ($self->_line_looks_like_record($line)) { |
161
|
|
|
|
|
|
|
# When the while reads the first line after the header section |
162
|
|
|
|
|
|
|
# we need to process it immediatelly because in zipped files we cannot go back |
163
|
31
|
|
|
|
|
245
|
my $record = $self->_parse_record_line($line); |
164
|
31
|
|
|
|
|
1649
|
$self->_add_record_in_cache($record); |
165
|
31
|
|
|
|
|
1236
|
return; |
166
|
|
|
|
|
|
|
} |
167
|
|
|
|
|
|
|
} |
168
|
|
|
|
|
|
|
} |
169
|
|
|
|
|
|
|
|
170
|
|
|
|
|
|
|
sub _next_record_from_file { |
171
|
10931
|
|
|
10931
|
|
16299
|
my ($self) = @_; |
172
|
|
|
|
|
|
|
|
173
|
10931
|
|
|
|
|
293422
|
while (my $line = $self->_filehandle->getline) { |
174
|
10914
|
50
|
|
|
|
260164
|
if ($self->_line_looks_like_record($line)) { |
|
|
0
|
|
|
|
|
|
175
|
10914
|
|
|
|
|
21518
|
return $self->_parse_record_line($line); |
176
|
|
|
|
|
|
|
} |
177
|
|
|
|
|
|
|
elsif ($self->_line_looks_like_header) { |
178
|
0
|
|
|
|
|
0
|
die "A record was expected but line looks like a header - the header should have been parsed already. $line\n"; |
179
|
|
|
|
|
|
|
} |
180
|
|
|
|
|
|
|
} |
181
|
|
|
|
|
|
|
|
182
|
17
|
|
|
|
|
1075
|
$self->_eof_reached(1); # When you reach this point the file has finished |
183
|
17
|
|
|
|
|
51
|
return undef; |
184
|
|
|
|
|
|
|
} |
185
|
|
|
|
|
|
|
|
186
|
|
|
|
|
|
|
sub _parse_record_line { |
187
|
10946
|
|
|
10946
|
|
17733
|
my ($self, $line) = @_; |
188
|
|
|
|
|
|
|
|
189
|
10946
|
|
|
|
|
14507
|
chomp $line; |
190
|
10946
|
|
|
|
|
13231
|
$line =~ s/(#.+)$//; |
191
|
10946
|
|
|
|
|
16693
|
my $comment_string = $1; |
192
|
10946
|
|
|
|
|
48576
|
my ($seqname, $source, $feature, $start, $end, $score, $strand, $frame, $attributes_string) = split(/\t/,$line); |
193
|
10946
|
|
|
|
|
51038
|
my @attributes = split(/;\s*/,$attributes_string); |
194
|
10946
|
|
|
|
|
10068
|
my %attributes_hash; |
195
|
10946
|
|
|
|
|
14407
|
foreach my $attribute (@attributes) { |
196
|
21892
|
|
|
|
|
59763
|
$attribute =~ /(.+)[=|\s]"(.+)"/; |
197
|
21892
|
|
|
|
|
57328
|
$attributes_hash{$1} = $2; |
198
|
|
|
|
|
|
|
} |
199
|
|
|
|
|
|
|
|
200
|
10946
|
|
|
|
|
398454
|
return GenOO::Data::File::GFF::Record->new({ |
201
|
|
|
|
|
|
|
seqname => $seqname, |
202
|
|
|
|
|
|
|
source => $source, |
203
|
|
|
|
|
|
|
feature => $feature, |
204
|
|
|
|
|
|
|
start_1_based => $start, # 1-based |
205
|
|
|
|
|
|
|
stop_1_based => $end, # 1-based |
206
|
|
|
|
|
|
|
score => $score, |
207
|
|
|
|
|
|
|
strand => $strand, |
208
|
|
|
|
|
|
|
frame => $frame, |
209
|
|
|
|
|
|
|
attributes => \%attributes_hash, |
210
|
|
|
|
|
|
|
comment => $comment_string, |
211
|
|
|
|
|
|
|
}); |
212
|
|
|
|
|
|
|
} |
213
|
|
|
|
|
|
|
|
214
|
|
|
|
|
|
|
sub _recognize_and_store_header_line { |
215
|
32
|
|
|
32
|
|
106
|
my ($self, $line) = @_; |
216
|
|
|
|
|
|
|
|
217
|
32
|
100
|
|
|
|
160
|
if ($self->_line_looks_like_version($line)) { |
218
|
16
|
|
|
|
|
132
|
$self->_parse_line_and_store_version($line); |
219
|
|
|
|
|
|
|
} |
220
|
|
|
|
|
|
|
else { |
221
|
16
|
|
|
|
|
102
|
$self->_parse_and_store_generic_header_line($line); |
222
|
|
|
|
|
|
|
} |
223
|
|
|
|
|
|
|
} |
224
|
|
|
|
|
|
|
|
225
|
|
|
|
|
|
|
sub _parse_line_and_store_version { |
226
|
16
|
|
|
16
|
|
185
|
my ($self, $line) = @_; |
227
|
|
|
|
|
|
|
|
228
|
16
|
|
|
|
|
182
|
my $version = (split(/\s+/,$line))[1]; |
229
|
16
|
|
|
|
|
799
|
$self->_header->{VERSION} = $version; |
230
|
|
|
|
|
|
|
} |
231
|
|
|
|
|
|
|
|
232
|
|
|
|
|
|
|
sub _parse_and_store_generic_header_line { |
233
|
16
|
|
|
16
|
|
63
|
my ($self, $line) = @_; |
234
|
|
|
|
|
|
|
|
235
|
16
|
|
|
|
|
139
|
my ($key, @values) = split(/\s+/,$line); |
236
|
16
|
|
|
|
|
901
|
$self->_header->{$key} = join(' ', @values); |
237
|
|
|
|
|
|
|
} |
238
|
|
|
|
|
|
|
|
239
|
|
|
|
|
|
|
sub _line_looks_like_header { |
240
|
145
|
|
|
145
|
|
2810
|
my ($self, $line) = @_; |
241
|
145
|
100
|
|
|
|
941
|
return ($line =~ /^#{2}/) ? 1 : 0; |
242
|
|
|
|
|
|
|
} |
243
|
|
|
|
|
|
|
|
244
|
|
|
|
|
|
|
sub _line_looks_like_record { |
245
|
11027
|
|
|
11027
|
|
16290
|
my ($self, $line) = @_; |
246
|
11027
|
100
|
|
|
|
37447
|
return ($line !~ /^#/) ? 1 : 0; |
247
|
|
|
|
|
|
|
} |
248
|
|
|
|
|
|
|
|
249
|
|
|
|
|
|
|
sub _line_looks_like_version { |
250
|
34
|
|
|
34
|
|
2633
|
my ($self, $line) = @_; |
251
|
34
|
100
|
|
|
|
295
|
return ($line =~ /^##gff-version/) ? 1 : 0; |
252
|
|
|
|
|
|
|
} |
253
|
|
|
|
|
|
|
|
254
|
|
|
|
|
|
|
|
255
|
|
|
|
|
|
|
####################################################################### |
256
|
|
|
|
|
|
|
######################### Private Methods ######################### |
257
|
|
|
|
|
|
|
####################################################################### |
258
|
|
|
|
|
|
|
sub _open_filehandle { |
259
|
31
|
|
|
31
|
|
47
|
my ($self) = @_; |
260
|
|
|
|
|
|
|
|
261
|
31
|
|
|
|
|
62
|
my $read_mode; |
262
|
|
|
|
|
|
|
my $HANDLE; |
263
|
31
|
50
|
|
|
|
993
|
if (!defined $self->file) { |
|
|
50
|
|
|
|
|
|
264
|
0
|
|
|
|
|
0
|
open ($HANDLE, '<-', $self->file); |
265
|
|
|
|
|
|
|
} |
266
|
|
|
|
|
|
|
elsif ($self->file =~ /\.gz$/) { |
267
|
31
|
50
|
|
|
|
796
|
die 'Cannot open file ' . $self->file . "\n" if ! -e $self->file; |
268
|
31
|
|
|
|
|
935
|
open($HANDLE, 'gzip -dc ' . $self->file . ' |'); |
269
|
|
|
|
|
|
|
} |
270
|
|
|
|
|
|
|
else { |
271
|
0
|
|
|
|
|
0
|
open ($HANDLE, '<', $self->file); |
272
|
|
|
|
|
|
|
} |
273
|
|
|
|
|
|
|
|
274
|
31
|
|
|
|
|
245431
|
return $HANDLE; |
275
|
|
|
|
|
|
|
} |
276
|
|
|
|
|
|
|
|
277
|
|
|
|
|
|
|
|
278
|
|
|
|
|
|
|
####################################################################### |
279
|
|
|
|
|
|
|
############################ Finalize ############################# |
280
|
|
|
|
|
|
|
####################################################################### |
281
|
|
|
|
|
|
|
__PACKAGE__->meta->make_immutable; |
282
|
|
|
|
|
|
|
|
283
|
|
|
|
|
|
|
1; |