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# POD documentation - main docs before the code |
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=head1 NAME |
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GenOO::Data::File::FASTQ - Object implementing methods for accessing fastq formatted files |
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=head1 SYNOPSIS |
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# Object that manages a fastq file. |
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# To initialize |
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my $file = GenOO::Data::File::FASTQ->new( |
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file => undef, |
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); |
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=head1 DESCRIPTION |
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This object offers methods to read a fastq file entry by entry |
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=head1 EXAMPLES |
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# Read one entry |
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my $entry = $fastq_parser->next_record(); |
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=cut |
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# Let the code begin... |
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package GenOO::Data::File::FASTQ; |
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$GenOO::Data::File::FASTQ::VERSION = '1.5.1'; |
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####################################################################### |
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####################### Load External modules ##################### |
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####################################################################### |
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use Modern::Perl; |
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use autodie; |
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use Moose; |
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use namespace::autoclean; |
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####################################################################### |
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######################### Load GenOO modules ###################### |
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####################################################################### |
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use GenOO::Data::File::FASTQ::Record; |
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####################################################################### |
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####################### Interface attributes ###################### |
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####################################################################### |
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has 'file' => ( |
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isa => 'Maybe[Str]', |
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is => 'rw', |
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required => 1 |
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); |
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has 'records_read_count' => ( |
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traits => ['Counter'], |
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is => 'ro', |
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isa => 'Num', |
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default => 0, |
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handles => { |
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_inc_records_read_count => 'inc', |
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_reset_records_read_count => 'reset', |
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}, |
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); |
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####################################################################### |
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######################## Private attributes ####################### |
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####################################################################### |
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has '_filehandle' => ( |
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is => 'ro', |
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builder => '_open_filehandle', |
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init_arg => undef, |
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lazy => 1, |
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); |
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####################################################################### |
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######################## Interface Methods ######################## |
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####################################################################### |
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sub next_record { |
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0
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1349
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my ($self) = @_; |
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85
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my $filehandle = $self->_filehandle; |
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1207
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while (my $line = $filehandle->getline) { |
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2097
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if ($line =~ /^\@/) { |
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my $id = substr($line,1); |
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chomp($id); |
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$id =~ s/\r//g; |
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1337
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my $seq = $filehandle->getline; |
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1686
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chomp($seq); |
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$seq =~ s/\r//g; |
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1200
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$filehandle->getline; #unused line |
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2429
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my $quality = $filehandle->getline; |
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1194
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chomp($quality); |
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$quality =~ s/\r//g; |
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2370
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$self->_inc_records_read_count; |
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return $self->_create_record($id, $seq, $quality); |
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} |
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} |
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1
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54
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return undef; |
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} |
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105
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106
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####################################################################### |
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107
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####################### Private Methods ############################ |
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108
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####################################################################### |
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109
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sub _open_filehandle { |
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2
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2
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my ($self) = @_; |
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111
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112
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my $read_mode; |
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my $HANDLE; |
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2
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92
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if (!defined $self->file) { |
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0
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0
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open ($HANDLE, '<-', $self->file); |
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} |
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elsif ($self->file =~ /\.gz$/) { |
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die 'Cannot open file ' . $self->file . "\n" if ! -e $self->file; |
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open($HANDLE, 'gzip -dc ' . $self->file . ' |'); |
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} |
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else { |
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0
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0
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open ($HANDLE, '<', $self->file); |
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} |
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125
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2
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22063
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return $HANDLE; |
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} |
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127
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128
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sub _create_record { |
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my ($self, $id, $seq, $quality) = @_; |
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131
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1856
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return GenOO::Data::File::FASTQ::Record->new( |
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name => $id, |
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133
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sequence => $seq, |
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134
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quality => $quality, |
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135
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); |
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136
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} |
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137
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138
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####################################################################### |
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139
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############################ Finalize ############################# |
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140
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####################################################################### |
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141
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__PACKAGE__->meta->make_immutable; |
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142
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143
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1; |