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package FASTX::ScriptHelper; |
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#ABSTRACT: Shared routines for binaries using FASTX::Reader and FASTX::PE. |
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4
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2
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2
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147990
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use 5.012; |
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2
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27
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5
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2
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2
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12
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use warnings; |
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2
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3
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2
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81
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6
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2
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2
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2604
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use File::Fetch; |
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2
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187855
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2
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83
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7
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2
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2
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20
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use Carp qw(confess cluck); |
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2
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4
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2
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123
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8
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2
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2
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1383
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use Data::Dumper; |
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2
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14289
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2
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142
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9
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2
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2
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1066
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use FASTX::Reader; |
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2
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10
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2
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103
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10
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2
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2
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15
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use File::Basename; |
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2
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4
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2
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108
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11
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2
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2
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14
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use File::Spec; |
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2
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3
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2
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82
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12
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2
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2
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1458
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use Term::ANSIColor qw(color); |
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2
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20961
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2
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1409
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13
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2
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2
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3362
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use JSON::PP; |
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2
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30047
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2
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156
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14
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2
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2
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3110
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use Capture::Tiny qw(capture); |
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2
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9676
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2
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128
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15
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2
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2
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14
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use Time::HiRes qw( time ); |
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2
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3
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2
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8
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16
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2
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2
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178
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use Scalar::Util qw( blessed refaddr reftype); |
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2
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4
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2
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4859
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17
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$FASTX::ScriptHelper::VERSION = '0.1.2'; |
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18
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19
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our @ISA = qw(Exporter); |
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20
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our @EXPORT = qw(rc fu_printfasta fu_printfastq verbose); |
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21
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our @EXPORT_OK = qw($fu_linesize $fu_verbose); # symbols to export on request |
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22
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23
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24
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sub new { |
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25
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26
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# Instantiate object |
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27
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3
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3
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1
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1195
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my ($class, $args) = @_; |
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28
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29
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3
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16
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my %accepted_parameters = ( |
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30
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'verbose' => 1, |
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31
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'debug' => 1, |
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32
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'logfile' => 1, |
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33
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'linesize'=> 1, |
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34
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); |
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35
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36
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3
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18
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my $valid_attributes = join(', ', keys %accepted_parameters); |
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37
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38
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3
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8
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for my $parameter (keys %{ $args} ) { |
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3
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14
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39
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confess("Attribute <$parameter> is not expected. Valid attributes are: $valid_attributes\n") |
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40
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7
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100
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309
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if (! $accepted_parameters{$parameter} ); |
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41
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} |
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42
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43
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44
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my $self = { |
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45
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logfile => $args->{logfile} // undef, |
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46
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debug => $args->{debug} // 0, |
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47
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verbose => $args->{verbose} // 0, |
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48
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2
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50
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41
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linesize => $args->{linesize} // 0, |
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50
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50
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50
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49
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}; |
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50
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2
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7
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my $object = bless $self, $class; |
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51
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52
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# Regular log file |
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53
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2
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50
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21
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if (defined $self->{logfile}) { |
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54
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2
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13
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verbose($self, "Ready to log in $object->{logfile}"); |
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55
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2
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33
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251
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open my $logfh, '>', "$object->{logfile}" || confess("ERROR: Unable to write log file to $object->{logfile}\n"); |
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56
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2
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17
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$object->{logfh} = $logfh; |
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57
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2
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8
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$object->{do_log} = 1; |
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58
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} else { |
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59
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# Set {logfh} to Stderr, but do not set {do_log} |
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60
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0
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0
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$object->{logfh} = *STDERR; |
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61
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} |
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62
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63
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2
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13
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return $object; |
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64
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} |
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65
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66
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67
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68
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sub fu_printfasta { |
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69
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70
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2
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2
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1
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478
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my $self = undef; |
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71
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2
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50
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10
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if ( ref($_[0]) eq 'FASTX::ScriptHelper' ) { |
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72
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2
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10
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$self = shift @_; |
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73
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} |
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74
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75
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2
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14
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my ($name, $comment, $seq) = @_; |
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76
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2
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50
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11
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confess("No sequence provided for $name") unless defined $seq; |
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77
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2
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11
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my $print_comment = ''; |
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78
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2
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50
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15
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if (defined $comment) { |
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79
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0
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0
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$print_comment = ' ' . $comment; |
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80
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} |
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81
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82
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2
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21
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say '>', $name, $print_comment; |
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83
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2
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50
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17
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if ($self) { |
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84
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2
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9
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print split_string($self,$seq); |
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85
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} else { |
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86
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0
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0
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print split_string($seq); |
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87
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} |
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88
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89
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} |
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90
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91
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92
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sub fu_printfastq { |
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93
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0
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0
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1
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0
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my $self = undef; |
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94
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0
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0
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0
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if ( ref($_[0]) eq 'FASTX::ScriptHelper' ) { |
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95
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0
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0
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$self = shift @_; |
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96
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} |
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97
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0
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0
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my ($name, $comment, $seq, $qual) = @_; |
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98
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0
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0
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my $print_comment = ''; |
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99
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0
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0
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0
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if (defined $comment) { |
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100
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0
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0
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$print_comment = ' ' . $comment; |
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101
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} |
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102
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0
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0
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0
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$qual = 'I' x length($seq) unless (defined $qual); |
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103
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0
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0
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say '@', $name, $print_comment; |
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104
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0
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0
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0
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if ($self) { |
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105
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0
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0
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print split_string($self,$seq) , "+\n", split_string($self,$qual); |
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106
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} else { |
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107
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0
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0
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print split_string($seq) , "+\n", split_string($qual); |
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108
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} |
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109
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110
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} |
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111
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112
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113
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sub rc { |
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114
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1
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1
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1
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919
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my $self = undef; |
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115
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1
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50
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5
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if ( ref($_[0]) eq 'FASTX::ScriptHelper' ) { |
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116
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1
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3
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$self = shift @_; |
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117
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} |
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118
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1
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3
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my $sequence = reverse($_[0]); |
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119
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1
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50
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3
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if (is_seq($sequence)) { |
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120
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1
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5
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$sequence =~tr/ACGTacgt/TGCAtgca/; |
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121
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1
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5
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return $sequence; |
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122
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} |
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123
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} |
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124
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125
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126
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sub is_seq { |
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127
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1
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1
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1
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2
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my $self = undef; |
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128
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1
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50
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3
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if ( ref($_[0]) eq 'FASTX::ScriptHelper' ) { |
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129
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0
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0
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$self = shift @_; |
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130
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} |
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131
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1
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2
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my $string = shift @_; |
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132
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1
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50
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5
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if ($string =~/[^ACGTRYSWKMBDHVN]/i) { |
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133
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0
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0
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return 0; |
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134
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} else { |
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135
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1
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34
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return 1; |
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136
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} |
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137
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} |
|
138
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139
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140
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sub split_string { |
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141
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2
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2
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1
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4
|
my $self = undef; |
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142
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2
|
50
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6
|
if ( ref($_[0]) eq 'FASTX::ScriptHelper' ) { |
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143
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2
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4
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$self = shift @_; |
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144
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} |
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145
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2
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8
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my $input_string = shift @_; |
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146
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2
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50
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15
|
confess("No string provided") unless $input_string; |
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147
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2
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12
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my $formatted = ''; |
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148
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2
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33
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13
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my $line_width = $self->{linesize} // $main::opt_line_size // 0; # change here |
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0
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149
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150
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2
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50
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11
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return $input_string. "\n" unless ($line_width); |
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151
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2
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18
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for (my $i = 0; $i < length($input_string); $i += $line_width) { |
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152
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2
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11
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my $frag = substr($input_string, $i, $line_width); |
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153
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2
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8
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$formatted .= $frag."\n"; |
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154
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} |
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155
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2
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9
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return $formatted; |
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156
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} |
|
157
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158
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159
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sub verbose { |
|
160
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2
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|
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2
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1
|
6
|
my $self = undef; |
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161
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2
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50
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9
|
if ( ref($_[0]) eq 'FASTX::ScriptHelper' ) { |
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162
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2
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7
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$self = shift @_; |
|
163
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} |
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164
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2
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|
7
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my ($message, $reference, $reference_name, @remainder) = @_; |
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165
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2
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50
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33
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17
|
if ($remainder[0]) { |
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50
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166
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0
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0
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$message .= $reference . $reference_name . join('', @remainder); |
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167
|
0
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0
|
$reference = undef; |
|
168
|
0
|
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0
|
$reference_name = undef; |
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169
|
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|
|
} elsif (defined $reference and reftype $reference eq undef) { |
|
170
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|
|
# Mistakenly passed list instead of string |
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171
|
0
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0
|
$message .= $reference; |
|
172
|
0
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0
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0
|
if (defined $reference_name) { |
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173
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0
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|
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0
|
$message .= $reference_name; |
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174
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0
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0
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$reference_name = undef; |
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175
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} |
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176
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0
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0
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$reference = undef; |
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177
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178
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} |
|
179
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2
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50
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12
|
my $variable_name = $reference_name // 'data'; |
|
180
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2
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9
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my $timestamp = _getTimeStamp(); |
|
181
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2
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50
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33
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18
|
if ( (defined $self and $self->{verbose} ) or (defined $main::opt_verbose and $main::opt_verbose) ) { |
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0
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33
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182
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# Print |
|
183
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2
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50
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8
|
if (defined $self->{do_log}) { |
|
184
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0
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0
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$self->writelog($message, $reference, $reference_name); |
|
185
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} |
|
186
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2
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14
|
say STDERR color('cyan'),"[$timestamp]", color('reset'), " $message"; |
|
187
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2
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50
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|
219
|
say STDERR color('magenta'), Data::Dumper->Dump([$reference], [$variable_name]) |
|
188
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|
|
if (defined $reference); |
|
189
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} else { |
|
190
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|
|
# No --verbose, don't print |
|
191
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0
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0
|
return -1; |
|
192
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} |
|
193
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194
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} |
|
195
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196
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197
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198
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|
|
sub writelog { |
|
199
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3
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3
|
1
|
893
|
my $self = undef; |
|
200
|
3
|
50
|
|
|
|
31
|
if ( ref($_[0]) eq 'FASTX::ScriptHelper' ) { |
|
201
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3
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|
13
|
$self = shift @_; |
|
202
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|
|
} |
|
203
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204
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3
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|
20
|
my ($message, $reference, $reference_name) = @_; |
|
205
|
3
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50
|
|
|
43
|
my $variable_name = $reference_name // 'data'; |
|
206
|
3
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|
|
|
|
15
|
my $timestamp = _getTimeStamp(); |
|
207
|
3
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|
|
|
|
106
|
say {$self->{logfh}} "[$timestamp] $message"; |
|
|
3
|
|
|
|
|
44
|
|
|
208
|
3
|
50
|
|
|
|
31
|
say {$self->{logfh}} Data::Dumper->Dump([$reference], [$variable_name]) if (defined $reference); |
|
|
0
|
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|
|
0
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|
|
209
|
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|
|
210
|
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|
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|
211
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|
|
} |
|
212
|
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|
213
|
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|
|
214
|
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|
|
215
|
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|
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|
216
|
|
|
|
|
|
|
sub download { |
|
217
|
0
|
|
|
0
|
1
|
0
|
my $begin_time = time(); |
|
218
|
0
|
|
|
|
|
0
|
my $self = undef; |
|
219
|
0
|
0
|
|
|
|
0
|
if ( ref($_[0]) eq 'FASTX::ScriptHelper' ) { |
|
220
|
0
|
|
|
|
|
0
|
$self = shift @_; |
|
221
|
|
|
|
|
|
|
} |
|
222
|
|
|
|
|
|
|
|
|
223
|
0
|
|
|
|
|
0
|
my ($url, $destination) = @_; |
|
224
|
0
|
0
|
|
|
|
0
|
if (defined $self->{do_log}) { |
|
225
|
0
|
|
|
|
|
0
|
$self->writelog( qq(Downloading "$url") ); |
|
226
|
|
|
|
|
|
|
} |
|
227
|
|
|
|
|
|
|
|
|
228
|
|
|
|
|
|
|
|
|
229
|
0
|
|
|
|
|
0
|
my $downloader = File::Fetch->new(uri => $url); |
|
230
|
0
|
0
|
|
|
|
0
|
my $file_path = $downloader->fetch( to => $destination ) or confess($downloader->error); |
|
231
|
0
|
|
|
|
|
0
|
my $end_time = time(); |
|
232
|
0
|
|
|
|
|
0
|
say Dumper $downloader; |
|
233
|
0
|
|
|
|
|
0
|
my $duration = sprintf("%.2fs", $end_time - $begin_time); |
|
234
|
0
|
|
|
|
|
0
|
return $file_path; |
|
235
|
|
|
|
|
|
|
} |
|
236
|
|
|
|
|
|
|
|
|
237
|
|
|
|
|
|
|
sub run { |
|
238
|
1
|
|
|
1
|
1
|
4
|
my $begin_time = time(); |
|
239
|
1
|
|
|
|
|
3
|
my $time_stamp = _getTimeStamp(); |
|
240
|
1
|
|
|
|
|
3
|
my $self = undef; |
|
241
|
1
|
50
|
|
|
|
5
|
if ( ref($_[0]) eq 'FASTX::ScriptHelper' ) { |
|
242
|
1
|
|
|
|
|
3
|
$self = shift @_; |
|
243
|
|
|
|
|
|
|
} |
|
244
|
1
|
|
|
|
|
4
|
my %valid_attributes = ( |
|
245
|
|
|
|
|
|
|
candie => 1, |
|
246
|
|
|
|
|
|
|
logall => 1, |
|
247
|
|
|
|
|
|
|
); |
|
248
|
|
|
|
|
|
|
|
|
249
|
|
|
|
|
|
|
|
|
250
|
1
|
|
|
|
|
3
|
my ($command, $options) = @_; |
|
251
|
1
|
|
|
|
|
3
|
_validate_attributes(\%valid_attributes, $options, 'run'); |
|
252
|
1
|
50
|
|
|
|
3
|
if (defined $self) { |
|
253
|
1
|
|
|
|
|
5
|
$self->writelog("Shell> $command"); |
|
254
|
|
|
|
|
|
|
} |
|
255
|
|
|
|
|
|
|
|
|
256
|
|
|
|
|
|
|
|
|
257
|
1
|
|
|
|
|
3
|
my $cmd = _runCmd($command); |
|
258
|
1
|
50
|
|
|
|
16
|
if ($cmd->{exit}) { |
|
259
|
0
|
|
|
|
|
0
|
$cmd->{failed} = 1; |
|
260
|
0
|
0
|
|
|
|
0
|
if (! $options->{candie}) { |
|
261
|
0
|
|
|
|
|
0
|
confess("Execution of an external command failed:\n$command"); |
|
262
|
|
|
|
|
|
|
} |
|
263
|
|
|
|
|
|
|
} |
|
264
|
1
|
|
|
|
|
14
|
my $end_time = time(); |
|
265
|
1
|
|
|
|
|
10
|
$cmd->{time} = $time_stamp; |
|
266
|
1
|
|
|
|
|
26
|
$cmd->{duration} = sprintf("%.2fs", $end_time - $begin_time); |
|
267
|
1
|
50
|
|
|
|
13
|
if (defined $self) { |
|
268
|
1
|
50
|
|
|
|
7
|
if ($options->{logall}) { |
|
269
|
0
|
|
|
|
|
0
|
$self->writelog(" +> Output: $cmd->{stdout}"); |
|
270
|
0
|
|
|
|
|
0
|
$self->writelog(" +> Messages: $cmd->{stderr}"); |
|
271
|
|
|
|
|
|
|
} |
|
272
|
1
|
|
|
|
|
26
|
$self->writelog(" +> Elapsed time: $cmd->{duration}; Exit status: $cmd->{exit};"); |
|
273
|
|
|
|
|
|
|
|
|
274
|
|
|
|
|
|
|
} |
|
275
|
|
|
|
|
|
|
|
|
276
|
1
|
|
|
|
|
13
|
return ($cmd); |
|
277
|
|
|
|
|
|
|
|
|
278
|
|
|
|
|
|
|
|
|
279
|
|
|
|
|
|
|
} |
|
280
|
|
|
|
|
|
|
|
|
281
|
|
|
|
|
|
|
|
|
282
|
|
|
|
|
|
|
sub cpu_count { |
|
283
|
1
|
50
|
|
1
|
1
|
709
|
if ( $^O =~ m/linux/i ) { |
|
|
|
0
|
|
|
|
|
|
|
284
|
1
|
|
|
|
|
7401
|
my($num) = qx(grep -c ^processor /proc/cpuinfo); |
|
285
|
1
|
50
|
|
|
|
94
|
return $1 if $num =~ m/^(\d+)/; |
|
286
|
|
|
|
|
|
|
} |
|
287
|
|
|
|
|
|
|
elsif ( $^O =~ m/darwin/i ) { |
|
288
|
0
|
|
|
|
|
0
|
my($num) = qx(system_profiler SPHardwareDataType | grep Cores); |
|
289
|
0
|
0
|
|
|
|
0
|
return $1 if $num =~ /.*Cores: (\d+)/; |
|
290
|
|
|
|
|
|
|
} |
|
291
|
0
|
|
|
|
|
0
|
return 1; |
|
292
|
|
|
|
|
|
|
} |
|
293
|
|
|
|
|
|
|
|
|
294
|
|
|
|
|
|
|
sub _getTimeStamp { |
|
295
|
|
|
|
|
|
|
|
|
296
|
6
|
|
|
6
|
|
251
|
my ($sec,$min,$hour,$mday,$mon,$year,$wday,$yday,$isdst)=localtime(time); |
|
297
|
6
|
|
|
|
|
69
|
my $timestamp = sprintf ( "%04d-%02d-%02d %02d:%02d:%02d", |
|
298
|
|
|
|
|
|
|
$year+1900,$mon+1,$mday,$hour,$min,$sec); |
|
299
|
6
|
|
|
|
|
22
|
return $timestamp; |
|
300
|
|
|
|
|
|
|
} |
|
301
|
|
|
|
|
|
|
|
|
302
|
|
|
|
|
|
|
|
|
303
|
|
|
|
|
|
|
sub _validate_attributes { |
|
304
|
1
|
|
|
1
|
|
2
|
my ($hash_ref, $options, $title) = @_; |
|
305
|
|
|
|
|
|
|
|
|
306
|
1
|
|
|
|
|
2
|
for my $attr (sort keys %{ $options } ) { |
|
|
1
|
|
|
|
|
6
|
|
|
307
|
1
|
50
|
|
|
|
1
|
confess "Invalid attribute '$attr' used calling routine '$title'\n" if (not defined ${ $hash_ref}{ $attr }); |
|
|
1
|
|
|
|
|
5
|
|
|
308
|
|
|
|
|
|
|
} |
|
309
|
1
|
|
|
|
|
2
|
return; |
|
310
|
|
|
|
|
|
|
} |
|
311
|
|
|
|
|
|
|
sub _runCmd { |
|
312
|
1
|
50
|
|
1
|
|
7
|
if ( ref($_[0]) eq 'FASTX::ScriptHelper' ) { |
|
313
|
0
|
|
|
|
|
0
|
shift @_; |
|
314
|
|
|
|
|
|
|
} |
|
315
|
1
|
|
|
|
|
3
|
my @cmd = @_; |
|
316
|
1
|
|
|
|
|
19
|
my $output; |
|
317
|
1
|
|
|
|
|
7
|
$output->{cmd} = join(' ', @cmd); |
|
318
|
|
|
|
|
|
|
|
|
319
|
|
|
|
|
|
|
my ($stdout, $stderr, $exit) = capture { |
|
320
|
1
|
|
|
1
|
|
5682
|
system( @cmd ); |
|
321
|
1
|
|
|
|
|
33
|
}; |
|
322
|
1
|
|
|
|
|
1409
|
chomp($stderr); |
|
323
|
1
|
|
|
|
|
10
|
chomp($stdout); |
|
324
|
1
|
|
|
|
|
10
|
$output->{stdout} = $stdout; |
|
325
|
1
|
|
|
|
|
3
|
$output->{stderr} = $stderr; |
|
326
|
1
|
|
|
|
|
9
|
$output->{exit} = $exit; |
|
327
|
|
|
|
|
|
|
|
|
328
|
1
|
|
|
|
|
15
|
return $output; |
|
329
|
|
|
|
|
|
|
} |
|
330
|
|
|
|
|
|
|
|
|
331
|
|
|
|
|
|
|
|
|
332
|
|
|
|
|
|
|
|
|
333
|
|
|
|
|
|
|
1; |
|
334
|
|
|
|
|
|
|
|
|
335
|
|
|
|
|
|
|
__END__ |