| line | stmt | bran | cond | sub | pod | time | code | 
| 1 |  |  |  |  |  |  | package BioX::Wrapper; | 
| 2 |  |  |  |  |  |  |  | 
| 3 | 1 |  |  | 1 |  | 16758 | use File::Find::Rule; | 
|  | 1 |  |  |  |  | 7498 |  | 
|  | 1 |  |  |  |  | 10 |  | 
| 4 | 1 |  |  | 1 |  | 54 | use File::Basename; | 
|  | 1 |  |  |  |  | 1 |  | 
|  | 1 |  |  |  |  | 94 |  | 
| 5 | 1 |  |  | 1 |  | 5 | use File::Path qw(make_path remove_tree); | 
|  | 1 |  |  |  |  | 7 |  | 
|  | 1 |  |  |  |  | 56 |  | 
| 6 | 1 |  |  | 1 |  | 4 | use File::Find::Rule; | 
|  | 1 |  |  |  |  | 1 |  | 
|  | 1 |  |  |  |  | 4 |  | 
| 7 | 1 |  |  | 1 |  | 25 | use Cwd; | 
|  | 1 |  |  |  |  | 1 |  | 
|  | 1 |  |  |  |  | 44 |  | 
| 8 | 1 |  |  | 1 |  | 961 | use DateTime; | 
|  | 1 |  |  |  |  | 156314 |  | 
|  | 1 |  |  |  |  | 56 |  | 
| 9 |  |  |  |  |  |  |  | 
| 10 | 1 |  |  | 1 |  | 951 | use Moose; | 
|  | 1 |  |  |  |  | 420549 |  | 
|  | 1 |  |  |  |  | 6 |  | 
| 11 | 1 |  |  | 1 |  | 5988 | use Moose::Util::TypeConstraints; | 
|  | 1 |  |  |  |  | 2 |  | 
|  | 1 |  |  |  |  | 9 |  | 
| 12 |  |  |  |  |  |  | with 'MooseX::Getopt'; | 
| 13 |  |  |  |  |  |  | with 'MooseX::Getopt::Usage'; | 
| 14 |  |  |  |  |  |  | with 'MooseX::Getopt::Usage::Role::Man'; | 
| 15 |  |  |  |  |  |  | with 'MooseX::SimpleConfig'; | 
| 16 |  |  |  |  |  |  |  | 
| 17 |  |  |  |  |  |  | # For pretty man pages! | 
| 18 |  |  |  |  |  |  | $ENV{TERM}='xterm-256color'; | 
| 19 |  |  |  |  |  |  |  | 
| 20 |  |  |  |  |  |  | our $VERSION = '1.5'; | 
| 21 |  |  |  |  |  |  |  | 
| 22 |  |  |  |  |  |  | =head1 BioX::Wrapper | 
| 23 |  |  |  |  |  |  |  | 
| 24 |  |  |  |  |  |  | Base class for BioX::Wrapper | 
| 25 |  |  |  |  |  |  |  | 
| 26 |  |  |  |  |  |  | =head2 Wrapper Options | 
| 27 |  |  |  |  |  |  |  | 
| 28 |  |  |  |  |  |  | =cut | 
| 29 |  |  |  |  |  |  |  | 
| 30 |  |  |  |  |  |  | =head3 example.yml | 
| 31 |  |  |  |  |  |  |  | 
| 32 |  |  |  |  |  |  | --- | 
| 33 |  |  |  |  |  |  | indir: "/path/to/files" | 
| 34 |  |  |  |  |  |  | outdir: "path/to/testdir" | 
| 35 |  |  |  |  |  |  |  | 
| 36 |  |  |  |  |  |  | =cut | 
| 37 |  |  |  |  |  |  |  | 
| 38 |  |  |  |  |  |  | has '+configfile' => ( | 
| 39 |  |  |  |  |  |  | required => 0, | 
| 40 |  |  |  |  |  |  | documentation => q{ | 
| 41 |  |  |  |  |  |  | If you get tired of putting all your options on the command line create a config file instead. | 
| 42 |  |  |  |  |  |  | --- | 
| 43 |  |  |  |  |  |  | indir: "/path/to/files" | 
| 44 |  |  |  |  |  |  | outdir: "path/to/testdir" | 
| 45 |  |  |  |  |  |  | } | 
| 46 |  |  |  |  |  |  | ); | 
| 47 |  |  |  |  |  |  |  | 
| 48 |  |  |  |  |  |  | =head3 comment_char | 
| 49 |  |  |  |  |  |  |  | 
| 50 |  |  |  |  |  |  | For a bash script a comment is "#", but is other characters for other languages | 
| 51 |  |  |  |  |  |  |  | 
| 52 |  |  |  |  |  |  | =cut | 
| 53 |  |  |  |  |  |  |  | 
| 54 |  |  |  |  |  |  | has 'comment_char' => ( | 
| 55 |  |  |  |  |  |  | is => 'rw', | 
| 56 |  |  |  |  |  |  | isa => 'Str', | 
| 57 |  |  |  |  |  |  | default => '#', | 
| 58 |  |  |  |  |  |  | ); | 
| 59 |  |  |  |  |  |  |  | 
| 60 |  |  |  |  |  |  | =head2 print_opts | 
| 61 |  |  |  |  |  |  |  | 
| 62 |  |  |  |  |  |  | Print out the command line options | 
| 63 |  |  |  |  |  |  |  | 
| 64 |  |  |  |  |  |  | =cut | 
| 65 |  |  |  |  |  |  |  | 
| 66 |  |  |  |  |  |  | sub print_opts { | 
| 67 | 0 |  |  | 0 |  |  | my($self) = @_; | 
| 68 |  |  |  |  |  |  |  | 
| 69 | 0 |  |  |  |  |  | my $now = DateTime->now(); | 
| 70 |  |  |  |  |  |  |  | 
| 71 | 0 |  |  |  |  |  | print "$self->{comment_char}\n"; | 
| 72 | 0 |  |  |  |  |  | print "$self->{comment_char} Generated at: $now\n"; | 
| 73 | 0 |  |  |  |  |  | print "$self->{comment_char} This file was generated with the following options\n"; | 
| 74 |  |  |  |  |  |  |  | 
| 75 | 0 |  |  |  |  |  | for(my $x=0; $x<=$#ARGV; $x++){ | 
| 76 | 0 | 0 |  |  |  |  | next unless $ARGV[$x]; | 
| 77 | 0 |  |  |  |  |  | print "$self->{comment_char}\t$ARGV[$x]\t"; | 
| 78 | 0 | 0 |  |  |  |  | if($ARGV[$x+1]){ | 
| 79 | 0 |  |  |  |  |  | print $ARGV[$x+1]; | 
| 80 |  |  |  |  |  |  | } | 
| 81 | 0 |  |  |  |  |  | print "\n"; | 
| 82 | 0 |  |  |  |  |  | $x++; | 
| 83 |  |  |  |  |  |  | } | 
| 84 |  |  |  |  |  |  |  | 
| 85 | 0 |  |  |  |  |  | print "$self->{comment_char}\n\n"; | 
| 86 |  |  |  |  |  |  | } | 
| 87 |  |  |  |  |  |  |  | 
| 88 |  |  |  |  |  |  | =head3 indir | 
| 89 |  |  |  |  |  |  |  | 
| 90 |  |  |  |  |  |  | A path to your vcf files can be given, and using File::Find::Rule it will recursively search for vcf or vcf.gz | 
| 91 |  |  |  |  |  |  |  | 
| 92 |  |  |  |  |  |  | =cut | 
| 93 |  |  |  |  |  |  |  | 
| 94 |  |  |  |  |  |  | has 'indir' => ( | 
| 95 |  |  |  |  |  |  | is => 'rw', | 
| 96 |  |  |  |  |  |  | isa => 'Str|Undef', | 
| 97 |  |  |  |  |  |  | required => 0, | 
| 98 |  |  |  |  |  |  | ); | 
| 99 |  |  |  |  |  |  |  | 
| 100 |  |  |  |  |  |  | =head3 outdir | 
| 101 |  |  |  |  |  |  |  | 
| 102 |  |  |  |  |  |  | Path to write out annotation files. It creates the structure | 
| 103 |  |  |  |  |  |  |  | 
| 104 |  |  |  |  |  |  | outdir | 
| 105 |  |  |  |  |  |  | --annovar_interim | 
| 106 |  |  |  |  |  |  | --annovar_final | 
| 107 |  |  |  |  |  |  | --vcf-annotate_interim #If you choose to reannotate VCF file | 
| 108 |  |  |  |  |  |  | --vcf-annotate_final #If you choose to reannotate VCF file | 
| 109 |  |  |  |  |  |  |  | 
| 110 |  |  |  |  |  |  | A lot of interim files are created by annovar, and the only one that really matters unless you debugging a new database is the multianno file found in annovar_final | 
| 111 |  |  |  |  |  |  |  | 
| 112 |  |  |  |  |  |  | If not given the outdirectory is assumed to be the current working directory. | 
| 113 |  |  |  |  |  |  |  | 
| 114 |  |  |  |  |  |  | =cut | 
| 115 |  |  |  |  |  |  |  | 
| 116 |  |  |  |  |  |  | has 'outdir' => ( | 
| 117 |  |  |  |  |  |  | is => 'rw', | 
| 118 |  |  |  |  |  |  | isa => 'Str', | 
| 119 |  |  |  |  |  |  | required => 1, | 
| 120 |  |  |  |  |  |  | default => sub { return getcwd() }, | 
| 121 |  |  |  |  |  |  | ); | 
| 122 |  |  |  |  |  |  |  | 
| 123 |  |  |  |  |  |  | 1; | 
| 124 |  |  |  |  |  |  | __END__ | 
| 125 |  |  |  |  |  |  |  | 
| 126 |  |  |  |  |  |  | =encoding utf-8 | 
| 127 |  |  |  |  |  |  |  | 
| 128 |  |  |  |  |  |  | =head1 NAME | 
| 129 |  |  |  |  |  |  |  | 
| 130 |  |  |  |  |  |  | BioX::Wrapper - Base class for BioX::Wrappers | 
| 131 |  |  |  |  |  |  |  | 
| 132 |  |  |  |  |  |  | =head1 SYNOPSIS | 
| 133 |  |  |  |  |  |  |  | 
| 134 |  |  |  |  |  |  | use BioX::Wrapper; | 
| 135 |  |  |  |  |  |  |  | 
| 136 |  |  |  |  |  |  | =head1 DESCRIPTION | 
| 137 |  |  |  |  |  |  |  | 
| 138 |  |  |  |  |  |  | BioX::Wrapper is | 
| 139 |  |  |  |  |  |  |  | 
| 140 |  |  |  |  |  |  | =head1 Acknowledgements | 
| 141 |  |  |  |  |  |  |  | 
| 142 |  |  |  |  |  |  | This module was originally developed at and for Weill Cornell Medical | 
| 143 |  |  |  |  |  |  | College in Qatar within ITS Advanced Computing Team. With approval from | 
| 144 |  |  |  |  |  |  | WCMC-Q, this information was generalized and put on github, for which | 
| 145 |  |  |  |  |  |  | the authors would like to express their gratitude. | 
| 146 |  |  |  |  |  |  |  | 
| 147 |  |  |  |  |  |  | =head1 AUTHOR | 
| 148 |  |  |  |  |  |  |  | 
| 149 |  |  |  |  |  |  | Jillian Rowe E<lt>jillian.e.rowe@gmail.comE<gt> | 
| 150 |  |  |  |  |  |  |  | 
| 151 |  |  |  |  |  |  | =head1 COPYRIGHT | 
| 152 |  |  |  |  |  |  |  | 
| 153 |  |  |  |  |  |  | Copyright 2015 - Weill Cornell Medical College in Qatar | 
| 154 |  |  |  |  |  |  |  | 
| 155 |  |  |  |  |  |  | =head1 LICENSE | 
| 156 |  |  |  |  |  |  |  | 
| 157 |  |  |  |  |  |  | This library is free software; you can redistribute it and/or modify | 
| 158 |  |  |  |  |  |  | it under the same terms as Perl itself. | 
| 159 |  |  |  |  |  |  |  | 
| 160 |  |  |  |  |  |  | =head1 SEE ALSO | 
| 161 |  |  |  |  |  |  |  | 
| 162 |  |  |  |  |  |  | =cut |