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package BioX::Workflow::Plugin::Drake; |
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our $VERSION = '0.12'; |
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use Data::Dumper; |
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use Data::Pairs; |
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use Moose::Role; |
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use Interpolation E => 'eval'; |
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=head1 NAME |
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BioX::Workflow::Plugin::Drake - A very opinionated template based bioinformatics workflow writer for Drake. |
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=head1 SYNOPSIS |
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The main documentation for this module is at L<BioX::Workflow>. This module extends Workflow in order to add functionality for outputing workflows in drake format. |
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biox-workflow.pl --workflow workflow.yml > workflow.drake |
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drake --workflow workflow.drake #with other functionality such as --jobs for asynchronous output, etc. |
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List your plugins in your workflow.yml file |
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--- |
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plugins: |
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- Drake |
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global: |
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- indir: /home/user/gemini |
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- outdir: /home/user/gemini/gemini-wrapper |
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- file_rule: (.vcf)$|(.vcf.gz)$ |
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- infile: |
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- min: 1 ##IF USING MIN |
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#So On and So Forth |
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More information about Drake can be found here L<https://github.com/Factual/drake>. |
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=head2 Default Variables |
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BioX::Workflow::Plugin::Drake assumes your INPUT/OUTPUT and indir/outdirs are |
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linked. |
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This means the output from step1 is the input for step2. |
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You can override this behavior by either declaring any of these values, or in the global |
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variables set auto_input: 0, disable automatic indir/outdir naming with |
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auto_name: 0, and disable automatically naming outdirectories by rule names with |
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enforce_struct: 0. |
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=head2 Example |
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=head3 workflow.yml |
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--- |
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plugins: |
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- Drake |
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global: |
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- indir: /home/user/workflow |
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- outdir: /home/user/workflow/output |
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- file_rule: (.csv)$ |
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rules: |
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- backup: |
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local: |
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- INPUT: "{$self->indir}/{$sample}.csv" |
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- OUTPUT: "{$self->outdir}/{$sample}.csv" |
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- thing: "other thing" |
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process: | |
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cp $INPUT $OUTPUT |
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- grep_VARA: |
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local: |
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- OUTPUT: "{$self->outdir}/{$sample}.grep_VARA.csv" |
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process: | |
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echo "Working on {$self->{indir}}/{$sample.csv}" |
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grep -i "VARA" {$self->indir}/{$sample}.csv >> {$self->outdir}/{$sample}.grep_VARA.csv \ |
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|| touch {$self->OUTPUT} |
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- grep_VARB: |
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local: |
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- OUTPUT: "{$self->outdir}/{$sample}.grep_VARA.grep_VARB.csv" |
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process: | |
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grep -i "VARB" {$self->indir}/{$sample}.grep_VARA.csv >> {$self->outdir}/{$sample}.grep_VARA.grep_VARB.csv || touch {$self->OUTPUT} |
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=head3 Notes on the drake.yml |
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Drake will stop everything if you're job returns with an exit code of anything |
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besides 0. For this reason we have the last command have a command1 || command2 |
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syntax, so that even if we don't grep any "VARB" from the file the workflow |
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could continue. |
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=head3 Run it with default setup |
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biox-workflow.pl --workflow workflow.yml > workflow.full.drake |
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=head3 Output with default setup |
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I don't want to inlcude the whole file, but you get the idea |
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; |
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; Generated at: 2015-06-21T11:01:24 |
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; This file was generated with the following options |
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; --workflow drake.yml |
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; --min 1 |
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; |
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; |
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; Samples: test1, test2 |
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; |
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; |
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; Starting Workflow |
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; |
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; |
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; Starting backup |
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; |
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; |
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; Variables |
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; Indir: /home/guests/jir2004/workflow |
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; Outdir: /home/guests/jir2004/workflow/output/backup |
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; Local Variables: |
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; INPUT: {$self->indir}/{$sample}.csv |
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; OUTPUT: {$self->outdir}/{$sample}.csv |
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; thing: other thing |
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; |
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/home/guests/jir2004/workflow/output/backup/$[SAMPLE].csv <- /home/guests/jir2004/workflow/$[SAMPLE].csv |
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cp $INPUT $OUTPUT |
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; |
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; Ending backup |
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; |
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; |
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; Starting grep_VARA |
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; |
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Run drake |
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drake --workflow workflow.full.drake |
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The following steps will be run, in order: |
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1: /home/user/workflow/output/backup/test1.csv <- /home/user/workflow/test1.csv [timestamped] |
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2: /home/user/workflow/output/backup/test2.csv <- /home/user/workflow/test2.csv [timestamped] |
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3: /home/user/workflow/output/grep_vara/test1.grep_VARA.csv <- /home/user/workflow/output/backup/test1.csv [projected timestamped] |
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4: /home/user/workflow/output/grep_vara/test2.grep_VARA.csv <- /home/user/workflow/output/backup/test2.csv [projected timestamped] |
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5: /home/user/workflow/output/grep_varb/test1.grep_VARA.grep_VARB.csv <- /home/user/workflow/output/grep_vara/test1.grep_VARA.csv [projected timestamped] |
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6: /home/user/workflow/output/grep_varb/test2.grep_VARA.grep_VARB.csv <- /home/user/workflow/output/grep_vara/test2.grep_VARA.csv [projected timestamped] |
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Confirm? [y/n] y |
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Running 6 steps with concurrence of 1... |
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--- 0. Running (timestamped): /home/user/workflow/output/backup/test1.csv <- /home/user/workflow/test1.csv |
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--- 0: /home/user/workflow/output/backup/test1.csv <- /home/user/workflow/test1.csv -> done in 0.02s |
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--- 1. Running (timestamped): /home/user/workflow/output/backup/test2.csv <- /home/user/workflow/test2.csv |
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--- 1: /home/user/workflow/output/backup/test2.csv <- /home/user/workflow/test2.csv -> done in 0.01s |
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--- 2. Running (timestamped): /home/user/workflow/output/grep_vara/test1.grep_VARA.csv <- /home/user/workflow/output/backup/test1.csv |
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Working on /home/user/workflow/output/backup/test1csv |
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--- 2: /home/user/workflow/output/grep_vara/test1.grep_VARA.csv <- /home/user/workflow/output/backup/test1.csv -> done in 0.01s |
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--- 3. Running (timestamped): /home/user/workflow/output/grep_vara/test2.grep_VARA.csv <- /home/user/workflow/output/backup/test2.csv |
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Working on /home/user/workflow/output/backup/test2csv |
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--- 3: /home/user/workflow/output/grep_vara/test2.grep_VARA.csv <- /home/user/workflow/output/backup/test2.csv -> done in 0.01s |
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--- 4. Running (timestamped): /home/user/workflow/output/grep_varb/test1.grep_VARA.grep_VARB.csv <- /home/user/workflow/output/grep_vara/test1.grep_VARA.csv |
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--- 4: /home/user/workflow/output/grep_varb/test1.grep_VARA.grep_VARB.csv <- /home/user/workflow/output/grep_vara/test1.grep_VARA.csv -> done in 0.01s |
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--- 5. Running (timestamped): /home/user/workflow/output/grep_varb/test2.grep_VARA.grep_VARB.csv <- /home/user/workflow/output/grep_vara/test2.grep_VARA.csv |
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--- 5: /home/user/workflow/output/grep_varb/test2.grep_VARA.grep_VARB.csv <- /home/user/workflow/output/grep_vara/test2.grep_VARA.csv -> done in 0.08s |
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Done (6 steps run). |
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=head3 Run in minified mode |
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As an alternative you can run this with the --min option, which instead of |
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printing out each workflow prints out only one, and creates a run-workflow.sh |
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which has all of your environmental variables. |
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This option is preferable if running on an HPC cluster with many nodes. |
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This WILL break with use of --resample, either local or global. You need to |
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split up your workflows as opposed to using the --resample option. |
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biox-workflow.pl --workflow workflow.yml --min 1 > workflow.drake #This also creates the run-workflow.sh in the same directory |
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./run-workflow.sh |
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cat drake.log #Here is the log for the first run |
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2015-06-21 14:02:47,543 INFO Running 3 steps with concurrence of 1... |
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2015-06-21 14:02:47,568 INFO |
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2015-06-21 14:02:47,570 INFO --- 0. Running (timestamped): /home/user/workflow/output/backup/test1.csv <- /home/user/workflow/test1.csv |
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2015-06-21 14:02:47,592 INFO --- 0: /home/user/workflow/output/backup/test1.csv <- /home/user/workflow/test1.csv -> done in 0.02s |
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#So on and so forth |
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If you look in the example directory you will see a few png files, these are outputs of the drake workflow. |
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=cut |
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203
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=head1 Acknowledgements |
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Before version 0.03 |
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This module was originally developed at and for Weill Cornell Medical |
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College in Qatar within ITS Advanced Computing Team. With approval from |
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WCMC-Q, this information was generalized and put on github, for which |
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the authors would like to express their gratitude. |
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As of version 0.03: |
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This modules continuing development is supported by NYU Abu Dhabi in the Center for Genomics and Systems Biology. |
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With approval from NYUAD, this information was generalized and put on bitbucket, for which |
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the authors would like to express their gratitude. |
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=head1 Inline Code Documentation |
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You shouldn't need these, but if you do here they are. |
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=head2 Attributes |
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=cut |
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=head3 full |
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Print the whole workflow hardcoded. This is the default |
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=cut |
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has 'full' => ( |
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is => 'rw', |
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isa => 'Bool', |
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default => 1, |
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); |
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=head3 min |
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Print the workflow as 2 files. |
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Run the drake things |
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drake --vars "SAMPLE=$sample" --workflow/workflow.drake |
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workflow.drake |
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Our regular file |
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=cut |
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has 'min' => ( |
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is => 'rw', |
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isa => 'Bool', |
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default => 0, |
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); |
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=head2 Subroutines |
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Subroutines |
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=head3 before run |
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Must initialize some variables |
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=cut |
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before 'run' => sub{ |
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my($self) = shift; |
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if($self->min){ |
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$self->full(0); |
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} |
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$self->wait(0); |
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$self->comment_char(';'); |
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}; |
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=head3 after get_samples |
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Things to do if we decide to do a min version |
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=cut |
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after 'get_samples' => sub{ |
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my($self) = shift; |
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return unless $self->min; |
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open(my $fh, '>', 'run-workflow.sh') or die print "Could not open file $!\n"; |
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print $fh "#!/bin/bash\n\n"; |
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foreach my $sample (@{$self->samples}){ |
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print $fh <<EOF; |
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drake --vars "SAMPLE=$sample" --workflow workflow.drake |
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EOF |
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} |
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close $fh; |
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chmod 0777, 'run-workflow.sh'; |
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$self->samples(["\$SAMPLE"]); |
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}; |
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=head3 write_process |
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Fill in the template with the process |
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Ensure INPUT/OUTPUT exist |
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Prettyify the output a bit |
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=cut |
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before 'write_process' => sub{ |
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my($self) = shift; |
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$DB::single=2; |
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if((! $self->local_attr->exists('INPUT')) && ! $self->local_attr->exists('OUTPUT') ){ |
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print "$self->{comment_char} There is no INPUT or OUTPUT!\n"; |
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} |
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#Make the formatting a big prettier |
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my @tmp = split("\n", $self->process); |
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$self->process(join("\n\t", @tmp)); |
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my $tmp = "{\$self->OUTPUT} <- {\$self->INPUT}\n\t"; |
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$DB::single=2; |
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if($self->min){ |
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$tmp =~ s/\$SAMPLE/\$[SAMPLE]/g; |
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} |
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my $newprocess = $tmp.$self->process; |
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$self->process($newprocess); |
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}; |
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#before 'process_template' => sub { |
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#my $self = shift; |
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#my $tmp = "{\$self->OUTPUT} <- {\$self->INPUT}\n\t"; |
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#$DB::single=2; |
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#if($self->min){ |
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#$tmp =~ s/\$SAMPLE/\$[SAMPLE]/g; |
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#} |
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#my $newprocess = $tmp.$self->process; |
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#$self->process($newprocess); |
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#}; |
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1; |
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__END__ |
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=encoding utf-8 |
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=head1 AUTHOR |
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Jillian Rowe E<lt>jillian.e.rowe@gmail.comE<gt> |
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=head1 COPYRIGHT |
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Copyright 2015- Jillian Rowe |
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=head1 LICENSE |
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This library is free software; you can redistribute it and/or modify |
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it under the same terms as Perl itself. |
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=head1 SEE ALSO |
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=cut |