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package BioUtil::Util;
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2
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3
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1
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1
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18364
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use File::Path qw/remove_tree/;
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1
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2
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1
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74
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4
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1
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1
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729
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use Time::HiRes qw/time/;
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1
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1380
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1
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4
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5
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6
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require Exporter;
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7
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@ISA = (Exporter);
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8
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@EXPORT = qw(
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9
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getopt
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10
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11
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file_list_from_argv
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12
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get_file_list
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13
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14
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delete_string_elements_by_indexes
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15
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delete_array_elements_by_indexes
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16
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17
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extract_parameters_from_string
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18
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get_parameters_from_file
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19
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20
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get_list_from_file
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21
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get_column_data
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22
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23
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read_json_file
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24
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write_json_file
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25
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26
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run
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27
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run_time
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28
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readable_second
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29
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30
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check_positive_integer
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31
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mean_and_stdev
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32
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33
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filename_prefix
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34
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check_all_files_exist
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35
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check_in_out_dir
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36
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rm_and_mkdir
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37
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38
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get_paired_fq_gz_file_from_dir
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39
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get_paired_fa_gz_file_from_dir
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40
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41
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);
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42
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43
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1
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1
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175
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use vars qw($VERSION);
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1
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1
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1
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44
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44
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45
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1
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1
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15
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use 5.010_000;
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1
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3
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46
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1
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1
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4
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use strict;
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1
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2
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1
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26
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47
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1
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1
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4
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use warnings FATAL => 'all';
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1
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2
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1
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53
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48
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49
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1
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1
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797
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use Encode qw/ encode_utf8 /;
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1
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10199
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1
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68
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50
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1
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1
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7
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use File::Path qw(make_path remove_tree);
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1
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1
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1
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44
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51
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1
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1
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5
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use File::Find;
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1
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2
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1
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59
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52
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1
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1
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11
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use File::Basename;
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1
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3
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1
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80
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53
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1
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1
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837
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use JSON;
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1
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15111
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1
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4
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54
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55
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=head1 NAME
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56
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57
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BioUtil::Util - Utilities for operation on data or file
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58
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59
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Some great modules like BioPerl provide many robust solutions.
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60
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However, it is not easy to install for someone in some platforms.
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61
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And for some simple task scripts, a lite module may be a good choice.
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62
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So I reinvented some wheels and added some useful utilities into this module,
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63
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hoping it would be helpful.
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64
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65
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=head1 VERSION
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66
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67
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Version 2015.0228
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68
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69
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=cut
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70
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71
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our $VERSION = 2015.0228;
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72
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73
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=head1 EXPORT
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74
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getopt
|
75
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76
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file_list_from_argv
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77
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get_file_list
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78
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79
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delete_string_elements_by_indexes
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80
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delete_array_elements_by_indexes
|
81
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82
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extract_parameters_from_string
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83
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get_parameters_from_file
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84
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85
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get_list_from_file
|
86
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get_column_data
|
87
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88
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read_json_file
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89
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write_json_file
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90
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91
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run
|
92
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run_time
|
93
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readable_second
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94
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95
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check_positive_integer
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96
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mean_and_stdev
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97
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98
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filename_prefix
|
99
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check_all_files_exist
|
100
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check_in_out_dir
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101
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rm_and_mkdir
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102
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103
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get_paired_fq_gz_file_from_dir
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104
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get_paired_fa_gz_file_from_dir
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105
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106
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=head1 SYNOPSIS
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107
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108
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use BioUtil::Util;
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109
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110
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111
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=head1 SUBROUTINES/METHODS
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112
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113
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=head2 getopt
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114
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115
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getopt FOR ME
|
116
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117
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Example
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118
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-a b -c t tt -d bb -dbtype asdfafd -test
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119
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120
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-a: b
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121
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-c: ARRAY(0xee25e8)
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122
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-d: bb
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123
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-dbtype: asdfafd
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124
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-infmt: fasta
|
125
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-test: 1
|
126
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127
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=cut
|
128
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129
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sub getopt {
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130
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0
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0
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1
|
0
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my ( $opts, $list ) = @_;
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131
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0
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0
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0
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0
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return "\$opts should be ref of hash, and \$list should be ref of list\n"
|
132
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unless ref $opts eq ref {}
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133
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and ref $list eq ref [];
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134
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0
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0
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my ( $o, $opt ) = (undef) x 2;
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135
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0
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0
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while (@$list) {
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136
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0
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0
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$o = shift @$list;
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137
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0
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0
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0
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if ( $o =~ /^\-/ ) {
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138
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0
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0
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$opt = $o;
|
139
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0
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0
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0
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$$opts{$opt} = 'http:shenwei.me' unless exists $$opts{$opt};
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140
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}
|
141
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else {
|
142
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0
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0
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0
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if ( $$opts{$opt} ne 'http:shenwei.me' ) {
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143
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$$opts{$opt} = [ $$opts{$opt} ]
|
144
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0
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0
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0
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if ref $$opts{$opt} ne ref [];
|
145
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0
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0
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push @{ $$opts{$opt} }, $o;
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0
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0
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146
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}
|
147
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else {
|
148
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0
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0
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$$opts{$opt} = $o;
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149
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}
|
150
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}
|
151
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}
|
152
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0
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0
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0
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for ( keys %$opts ) { $$opts{$_} = 1 if $$opts{$_} eq 'http:shenwei.me'; }
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0
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0
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153
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0
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0
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return $opts;
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154
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}
|
155
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156
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=head2 file_list_from_argv
|
157
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158
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Get file list from @ARGV. You should use this after parsing options!
|
159
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160
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When no arguments given, 'STDIN' will be added to
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161
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the list, which could be further used by, e.g. FastaReader.
|
162
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|
163
|
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=cut
|
164
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165
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sub file_list_from_argv {
|
166
|
0
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|
|
0
|
1
|
0
|
my @files = ();
|
167
|
0
|
|
|
|
|
0
|
for my $file (@_) {
|
168
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0
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0
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for my $f ( glob $file ) {
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169
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0
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0
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push @files, $f;
|
170
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}
|
171
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}
|
172
|
0
|
0
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0
|
if ( @files == 0 ) {
|
173
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0
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0
|
push @files, 'STDIN';
|
174
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}
|
175
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0
|
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0
|
return @files;
|
176
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}
|
177
|
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|
178
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=head2 get_file_list
|
179
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|
180
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Find files/directories with custom filter,
|
181
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max serach depth could be specified.
|
182
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183
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Example (searching perl scripts)
|
184
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|
185
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my $dir = "~";
|
186
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my $depth = 2;
|
187
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|
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|
188
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my $list = get_file_list(
|
189
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|
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$dir,
|
190
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sub {
|
191
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if ( -d or /^\./i ) { # ignore configuration file and folders
|
192
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return 0;
|
193
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|
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}
|
194
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if (/\.pm/i or /\.pl/i) {
|
195
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return 1;
|
196
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}
|
197
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return 0;
|
198
|
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},
|
199
|
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|
|
$depth
|
200
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|
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);
|
201
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print "$_\n" for @$list;
|
202
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|
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|
203
|
|
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|
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=cut
|
204
|
|
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205
|
|
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|
|
sub get_file_list {
|
206
|
|
|
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|
207
|
|
|
|
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|
|
# filter is a subroutine to filter a file
|
208
|
1
|
|
|
1
|
1
|
11
|
my ( $dir, $filter, $depth ) = @_;
|
209
|
1
|
|
|
|
|
7
|
$dir =~ s/\/+/\//g;
|
210
|
1
|
|
|
|
|
5
|
$dir =~ s/\/$//;
|
211
|
|
|
|
|
|
|
|
212
|
1
|
50
|
|
|
|
4
|
$depth = 1 << 30 unless defined $depth;
|
213
|
1
|
50
|
33
|
|
|
14
|
unless ( $depth =~ /^\d+$/ and $depth > 0 ) {
|
214
|
0
|
|
|
|
|
0
|
warn "depth should be positive integer\n";
|
215
|
0
|
|
|
|
|
0
|
return [];
|
216
|
|
|
|
|
|
|
}
|
217
|
|
|
|
|
|
|
|
218
|
|
|
|
|
|
|
# print "$dir\n";
|
219
|
1
|
|
|
|
|
4
|
my $depth0 = $dir =~ tr/\//\//;
|
220
|
|
|
|
|
|
|
|
221
|
1
|
|
|
|
|
2
|
my $files = [];
|
222
|
|
|
|
|
|
|
my $wanted = sub {
|
223
|
56
|
100
|
|
56
|
|
955
|
return if /^\.+$/;
|
224
|
55
|
50
|
|
|
|
117
|
return if $_ eq $dir;
|
225
|
|
|
|
|
|
|
|
226
|
|
|
|
|
|
|
# check depth
|
227
|
55
|
100
|
|
|
|
1253
|
return if $File::Find::name =~ tr/\//\// - $depth0 > $depth;
|
228
|
|
|
|
|
|
|
|
229
|
14
|
100
|
|
|
|
32
|
if ( &$filter($_) ) {
|
230
|
1
|
|
|
|
|
28
|
push @$files, $File::Find::name;
|
231
|
|
|
|
|
|
|
}
|
232
|
1
|
|
|
|
|
6
|
};
|
233
|
|
|
|
|
|
|
|
234
|
1
|
|
|
|
|
88
|
find( $wanted, ($dir) );
|
235
|
|
|
|
|
|
|
|
236
|
1
|
|
|
|
|
8
|
return $files;
|
237
|
|
|
|
|
|
|
}
|
238
|
|
|
|
|
|
|
|
239
|
|
|
|
|
|
|
=head2 delete_string_elements_by_indexes
|
240
|
|
|
|
|
|
|
|
241
|
|
|
|
|
|
|
Delete string elements by indexes, it uses delete_array_elements_by_indexes
|
242
|
|
|
|
|
|
|
|
243
|
|
|
|
|
|
|
=cut
|
244
|
|
|
|
|
|
|
|
245
|
|
|
|
|
|
|
sub delete_string_elements_by_indexes {
|
246
|
0
|
|
|
0
|
1
|
0
|
my ( $str, $ids ) = @_;
|
247
|
0
|
|
|
|
|
0
|
my $t = '';
|
248
|
0
|
0
|
0
|
|
|
0
|
unless ( ref $str eq ref \$t and ref $ids eq ref [] ) {
|
249
|
0
|
|
|
|
|
0
|
die "both arguments should be array reference\n";
|
250
|
|
|
|
|
|
|
}
|
251
|
0
|
|
|
|
|
0
|
my @bytes = split //, $$str;
|
252
|
0
|
|
|
|
|
0
|
return join "", @{ delete_array_elements_by_indexes( \@bytes, $ids ) };
|
|
0
|
|
|
|
|
0
|
|
253
|
|
|
|
|
|
|
}
|
254
|
|
|
|
|
|
|
|
255
|
|
|
|
|
|
|
=head2 delete_array_elements_by_indexes
|
256
|
|
|
|
|
|
|
|
257
|
|
|
|
|
|
|
Delete array elements by given indexes.
|
258
|
|
|
|
|
|
|
|
259
|
|
|
|
|
|
|
Example:
|
260
|
|
|
|
|
|
|
|
261
|
|
|
|
|
|
|
@list = qw(a b c d e f);
|
262
|
|
|
|
|
|
|
@idx = (1, 2, 4);
|
263
|
|
|
|
|
|
|
$list2 = delete_array_elements_by_indexes(\@list, \@idx);
|
264
|
|
|
|
|
|
|
print "@$list2\n"; # result: a, d, f
|
265
|
|
|
|
|
|
|
|
266
|
|
|
|
|
|
|
=cut
|
267
|
|
|
|
|
|
|
|
268
|
|
|
|
|
|
|
sub delete_array_elements_by_indexes {
|
269
|
0
|
|
|
0
|
1
|
0
|
my ( $array, $ids ) = @_;
|
270
|
0
|
0
|
0
|
|
|
0
|
unless ( ref $array eq ref [] and ref $ids eq ref [] ) {
|
271
|
0
|
|
|
|
|
0
|
die "both arguments should be array reference\n";
|
272
|
|
|
|
|
|
|
}
|
273
|
0
|
|
|
|
|
0
|
my %omitted = map { $_ => 1 } @$ids;
|
|
0
|
|
|
|
|
0
|
|
274
|
0
|
|
|
|
|
0
|
my @newarray = ();
|
275
|
0
|
|
|
|
|
0
|
for my $i ( 0 .. ( scalar(@$array) - 1 ) ) {
|
276
|
0
|
0
|
|
|
|
0
|
next if exists $omitted{$i};
|
277
|
0
|
|
|
|
|
0
|
push @newarray, $$array[$i];
|
278
|
|
|
|
|
|
|
}
|
279
|
0
|
|
|
|
|
0
|
return \@newarray;
|
280
|
|
|
|
|
|
|
}
|
281
|
|
|
|
|
|
|
|
282
|
|
|
|
|
|
|
=head2 extract_parameters_from_string
|
283
|
|
|
|
|
|
|
|
284
|
|
|
|
|
|
|
Extract parameters from string.
|
285
|
|
|
|
|
|
|
|
286
|
|
|
|
|
|
|
The regular expression is
|
287
|
|
|
|
|
|
|
|
288
|
|
|
|
|
|
|
/([\w\d\_\-\.]+)\s*=\s*([^\=;]*)[\s;]*/
|
289
|
|
|
|
|
|
|
|
290
|
|
|
|
|
|
|
Example:
|
291
|
|
|
|
|
|
|
|
292
|
|
|
|
|
|
|
# bad format, but could also be parsed
|
293
|
|
|
|
|
|
|
# my $s = " s = b; a=test; b_c=12 3; a.b =; b
|
294
|
|
|
|
|
|
|
# = asdf
|
295
|
|
|
|
|
|
|
# sd; ads-f = 12313";
|
296
|
|
|
|
|
|
|
|
297
|
|
|
|
|
|
|
# recommended
|
298
|
|
|
|
|
|
|
my $s = "key1=abcde; key2=123; conf.a=file; conf.b=12; ";
|
299
|
|
|
|
|
|
|
|
300
|
|
|
|
|
|
|
my $pa = extract_parameters_from_string($s);
|
301
|
|
|
|
|
|
|
print "=$_:$$p{$_}=\n" for sort keys %$pa;
|
302
|
|
|
|
|
|
|
|
303
|
|
|
|
|
|
|
=cut
|
304
|
|
|
|
|
|
|
|
305
|
|
|
|
|
|
|
sub extract_parameters_from_string {
|
306
|
1
|
|
|
1
|
1
|
786
|
my ($s) = @_;
|
307
|
1
|
|
|
|
|
3
|
my $parameters = {};
|
308
|
1
|
|
|
|
|
11
|
while ( $s =~ /([\w\d\_\-\.]+)\s*=\s*([^\=;]*)[\s;]*/gm ) {
|
309
|
4
|
50
|
|
|
|
15
|
warn "$1 was defined more than once\n" if defined $$parameters{$1};
|
310
|
4
|
|
|
|
|
24
|
$$parameters{$1} = $2;
|
311
|
|
|
|
|
|
|
}
|
312
|
1
|
|
|
|
|
3
|
return $parameters;
|
313
|
|
|
|
|
|
|
}
|
314
|
|
|
|
|
|
|
|
315
|
|
|
|
|
|
|
=head2 get_parameters_from_file
|
316
|
|
|
|
|
|
|
|
317
|
|
|
|
|
|
|
Get parameters from a file.
|
318
|
|
|
|
|
|
|
Comments start with # are allowed in file.
|
319
|
|
|
|
|
|
|
|
320
|
|
|
|
|
|
|
Example:
|
321
|
|
|
|
|
|
|
|
322
|
|
|
|
|
|
|
my $pa = get_parameters_from_file("d.txt");
|
323
|
|
|
|
|
|
|
print "$_: $$pa{$_}\n" for sort keys %$pa;
|
324
|
|
|
|
|
|
|
|
325
|
|
|
|
|
|
|
For a file with content:
|
326
|
|
|
|
|
|
|
|
327
|
|
|
|
|
|
|
# cell phone
|
328
|
|
|
|
|
|
|
apple = 1 # note
|
329
|
|
|
|
|
|
|
|
330
|
|
|
|
|
|
|
nokia = 2 #
|
331
|
|
|
|
|
|
|
|
332
|
|
|
|
|
|
|
output is:
|
333
|
|
|
|
|
|
|
|
334
|
|
|
|
|
|
|
apple: 1
|
335
|
|
|
|
|
|
|
nokia: 2
|
336
|
|
|
|
|
|
|
|
337
|
|
|
|
|
|
|
=cut
|
338
|
|
|
|
|
|
|
|
339
|
|
|
|
|
|
|
sub get_parameters_from_file {
|
340
|
1
|
|
|
1
|
1
|
432
|
my ($file) = @_;
|
341
|
1
|
|
|
|
|
3
|
my $parameters = {};
|
342
|
1
|
50
|
|
|
|
41
|
open my $fh, $file or die "fail to open file $file\n";
|
343
|
1
|
|
|
|
|
22
|
while (<$fh>) {
|
344
|
4
|
|
|
|
|
22
|
s/^\s+|\s+$//g;
|
345
|
4
|
100
|
100
|
|
|
30
|
next if $_ eq '' # blank line
|
346
|
|
|
|
|
|
|
or /^#/; # annotation
|
347
|
2
|
|
|
|
|
8
|
s/#.*//g; # delete annotation
|
348
|
|
|
|
|
|
|
|
349
|
2
|
50
|
|
|
|
11
|
next unless /([\w\d\_\-\.]+)\s*=\s*(.+)/;
|
350
|
2
|
|
|
|
|
16
|
$$parameters{$1} = $2;
|
351
|
|
|
|
|
|
|
}
|
352
|
1
|
|
|
|
|
9
|
close $fh;
|
353
|
1
|
|
|
|
|
5
|
return $parameters;
|
354
|
|
|
|
|
|
|
}
|
355
|
|
|
|
|
|
|
|
356
|
|
|
|
|
|
|
=head2 get_list_from_file
|
357
|
|
|
|
|
|
|
|
358
|
|
|
|
|
|
|
Get list from a file.
|
359
|
|
|
|
|
|
|
Comments start with # are allowed in file.
|
360
|
|
|
|
|
|
|
|
361
|
|
|
|
|
|
|
Example:
|
362
|
|
|
|
|
|
|
|
363
|
|
|
|
|
|
|
my $list = get_list_from_file("d.txt");
|
364
|
|
|
|
|
|
|
print "$_\n" for @$list;
|
365
|
|
|
|
|
|
|
|
366
|
|
|
|
|
|
|
For a file with content:
|
367
|
|
|
|
|
|
|
|
368
|
|
|
|
|
|
|
# cell phone
|
369
|
|
|
|
|
|
|
apple # note
|
370
|
|
|
|
|
|
|
|
371
|
|
|
|
|
|
|
nokia
|
372
|
|
|
|
|
|
|
|
373
|
|
|
|
|
|
|
output is:
|
374
|
|
|
|
|
|
|
|
375
|
|
|
|
|
|
|
apple
|
376
|
|
|
|
|
|
|
nokia
|
377
|
|
|
|
|
|
|
|
378
|
|
|
|
|
|
|
=cut
|
379
|
|
|
|
|
|
|
|
380
|
|
|
|
|
|
|
sub get_list_from_file {
|
381
|
1
|
|
|
1
|
1
|
490
|
my ($file) = @_;
|
382
|
1
|
50
|
|
|
|
46
|
open my $fh, "<", $file or die "fail to open file $file\n";
|
383
|
1
|
|
|
|
|
3
|
my @list = ();
|
384
|
1
|
|
|
|
|
13
|
while (<$fh>) {
|
385
|
4
|
|
|
|
|
19
|
s/\r?\n//g;
|
386
|
4
|
|
|
|
|
18
|
s/^\s+|\s+$//g;
|
387
|
4
|
100
|
100
|
|
|
27
|
next if $_ eq '' # blank line
|
388
|
|
|
|
|
|
|
or /^#/; # annotation
|
389
|
2
|
|
|
|
|
7
|
s/#.*//g; # delete annotation
|
390
|
|
|
|
|
|
|
|
391
|
2
|
|
|
|
|
10
|
push @list, $_;
|
392
|
|
|
|
|
|
|
}
|
393
|
1
|
|
|
|
|
9
|
close $fh;
|
394
|
1
|
|
|
|
|
7
|
return \@list;
|
395
|
|
|
|
|
|
|
}
|
396
|
|
|
|
|
|
|
|
397
|
|
|
|
|
|
|
=head2 get_column_data
|
398
|
|
|
|
|
|
|
|
399
|
|
|
|
|
|
|
Get one column of a file.
|
400
|
|
|
|
|
|
|
|
401
|
|
|
|
|
|
|
Example:
|
402
|
|
|
|
|
|
|
|
403
|
|
|
|
|
|
|
my $list = get_column_data("d.txt", 2);
|
404
|
|
|
|
|
|
|
print "$_\n" for @$list;
|
405
|
|
|
|
|
|
|
|
406
|
|
|
|
|
|
|
=cut
|
407
|
|
|
|
|
|
|
|
408
|
|
|
|
|
|
|
sub get_column_data {
|
409
|
1
|
|
|
1
|
1
|
425
|
my ( $file, $column, $delimiter ) = @_;
|
410
|
1
|
50
|
33
|
|
|
14
|
unless ( $column =~ /^(\d+)$/ and $column > 0 ) {
|
411
|
0
|
|
|
|
|
0
|
warn "column number ($column) should be positive integer\n";
|
412
|
0
|
|
|
|
|
0
|
$column = 1;
|
413
|
|
|
|
|
|
|
}
|
414
|
1
|
50
|
|
|
|
5
|
$delimiter = "\t" unless defined $delimiter;
|
415
|
|
|
|
|
|
|
|
416
|
1
|
50
|
|
|
|
35
|
open my $fh, "<", $file or die "failed to open file: $file\n";
|
417
|
1
|
|
|
|
|
3
|
my @linedata = ();
|
418
|
1
|
|
|
|
|
2
|
my @data = ();
|
419
|
1
|
|
|
|
|
2
|
my $n = 0;
|
420
|
1
|
|
|
|
|
13
|
while (<$fh>) {
|
421
|
4
|
|
|
|
|
19
|
s/\r?\n//;
|
422
|
4
|
50
|
|
|
|
13
|
next if /^\s*#/;
|
423
|
4
|
|
|
|
|
27
|
@linedata = split /$delimiter/, $_;
|
424
|
4
|
|
|
|
|
7
|
$n = scalar @linedata;
|
425
|
4
|
100
|
|
|
|
15
|
next unless $n > 0;
|
426
|
|
|
|
|
|
|
|
427
|
3
|
50
|
|
|
|
7
|
if ( $column > $n ) {
|
428
|
0
|
|
|
|
|
0
|
die
|
429
|
|
|
|
|
|
|
"number of columns of this line ($n) is less than given column number ($column)\n$_";
|
430
|
|
|
|
|
|
|
}
|
431
|
|
|
|
|
|
|
|
432
|
3
|
|
|
|
|
13
|
push @data, $linedata[ $column - 1 ];
|
433
|
|
|
|
|
|
|
}
|
434
|
1
|
|
|
|
|
9
|
close $fh;
|
435
|
|
|
|
|
|
|
|
436
|
1
|
|
|
|
|
6
|
return \@data;
|
437
|
|
|
|
|
|
|
}
|
438
|
|
|
|
|
|
|
|
439
|
|
|
|
|
|
|
=head2 read_json_file
|
440
|
|
|
|
|
|
|
|
441
|
|
|
|
|
|
|
Read json file and decode it into a hash ref.
|
442
|
|
|
|
|
|
|
|
443
|
|
|
|
|
|
|
Example:
|
444
|
|
|
|
|
|
|
|
445
|
|
|
|
|
|
|
my $hashref = read_json_file($file);
|
446
|
|
|
|
|
|
|
|
447
|
|
|
|
|
|
|
=cut
|
448
|
|
|
|
|
|
|
|
449
|
|
|
|
|
|
|
sub read_json_file {
|
450
|
1
|
|
|
1
|
1
|
6
|
my ($file) = @_;
|
451
|
1
|
50
|
|
|
|
30
|
open my $fh, "<:encoding(utf8)", $file
|
452
|
|
|
|
|
|
|
or die "fail to open json file: $file\n";
|
453
|
1
|
|
|
|
|
41
|
my $text;
|
454
|
1
|
|
|
|
|
20
|
while (<$fh>) {
|
455
|
5
|
|
|
|
|
27
|
s/\s*#+.*\r?\n?//g; # remove annotation
|
456
|
5
|
50
|
|
|
|
40
|
$text .= $1 if / *(.+)/;
|
457
|
|
|
|
|
|
|
}
|
458
|
1
|
|
|
|
|
11
|
close $fh;
|
459
|
1
|
|
|
|
|
12
|
my $hash = decode_json($text);
|
460
|
1
|
|
|
|
|
5
|
return $hash;
|
461
|
|
|
|
|
|
|
}
|
462
|
|
|
|
|
|
|
|
463
|
|
|
|
|
|
|
=head2 write_json_file
|
464
|
|
|
|
|
|
|
|
465
|
|
|
|
|
|
|
Write a hash ref into a file.
|
466
|
|
|
|
|
|
|
|
467
|
|
|
|
|
|
|
Example:
|
468
|
|
|
|
|
|
|
|
469
|
|
|
|
|
|
|
my $hashref = { "a" => 1, "b" => 2 };
|
470
|
|
|
|
|
|
|
write_json_file($hashref, $file);
|
471
|
|
|
|
|
|
|
|
472
|
|
|
|
|
|
|
=cut
|
473
|
|
|
|
|
|
|
|
474
|
|
|
|
|
|
|
sub write_json_file {
|
475
|
1
|
|
|
1
|
1
|
421
|
my ( $hash, $file ) = @_;
|
476
|
1
|
|
|
|
|
22
|
my $json = JSON->new->allow_nonref;
|
477
|
1
|
|
|
|
|
35
|
my $text = $json->pretty->encode($hash);
|
478
|
1
|
|
|
|
|
6
|
$text = encode_utf8($text);
|
479
|
1
|
50
|
|
1
|
|
6
|
open my $fh2, ">:encoding(utf8)", $file
|
|
1
|
|
|
|
|
2
|
|
|
1
|
|
|
|
|
7
|
|
|
1
|
|
|
|
|
45
|
|
480
|
|
|
|
|
|
|
or die "fail to open json file: $file\n";
|
481
|
1
|
|
|
|
|
1673
|
print $fh2 $text;
|
482
|
1
|
|
|
|
|
99
|
close $fh2;
|
483
|
|
|
|
|
|
|
}
|
484
|
|
|
|
|
|
|
|
485
|
|
|
|
|
|
|
=head2 run
|
486
|
|
|
|
|
|
|
|
487
|
|
|
|
|
|
|
Run a command
|
488
|
|
|
|
|
|
|
|
489
|
|
|
|
|
|
|
Example:
|
490
|
|
|
|
|
|
|
|
491
|
|
|
|
|
|
|
my $fail = run($cmd);
|
492
|
|
|
|
|
|
|
die "failed to run:$cmd\n" if $fail;
|
493
|
|
|
|
|
|
|
|
494
|
|
|
|
|
|
|
=cut
|
495
|
|
|
|
|
|
|
|
496
|
|
|
|
|
|
|
sub run {
|
497
|
0
|
|
|
0
|
1
|
|
my ($cmd) = @_;
|
498
|
0
|
|
|
|
|
|
system($cmd);
|
499
|
|
|
|
|
|
|
|
500
|
0
|
0
|
|
|
|
|
if ( $? == -1 ) {
|
|
|
0
|
|
|
|
|
|
501
|
0
|
|
|
|
|
|
die "[ERROR] fail to run: $cmd. Command ("
|
502
|
|
|
|
|
|
|
. ( split /\s+/, $cmd )[0]
|
503
|
|
|
|
|
|
|
. ") not found\n";
|
504
|
|
|
|
|
|
|
}
|
505
|
|
|
|
|
|
|
elsif ( $? & 127 ) {
|
506
|
0
|
0
|
|
|
|
|
printf STDERR "[ERROR] command died with signal %d, %s coredump\n",
|
507
|
|
|
|
|
|
|
( $? & 127 ), ( $? & 128 ) ? 'with' : 'without';
|
508
|
|
|
|
|
|
|
}
|
509
|
|
|
|
|
|
|
else {
|
510
|
|
|
|
|
|
|
# 0, ok
|
511
|
|
|
|
|
|
|
}
|
512
|
0
|
|
|
|
|
|
return $?;
|
513
|
|
|
|
|
|
|
}
|
514
|
|
|
|
|
|
|
|
515
|
|
|
|
|
|
|
=head2 run_time
|
516
|
|
|
|
|
|
|
|
517
|
|
|
|
|
|
|
Run a subroutine with given arguments N times, and return the mean and stdev
|
518
|
|
|
|
|
|
|
of time.
|
519
|
|
|
|
|
|
|
|
520
|
|
|
|
|
|
|
Example:
|
521
|
|
|
|
|
|
|
|
522
|
|
|
|
|
|
|
my $read_by_record = sub {
|
523
|
|
|
|
|
|
|
my ($file) = @_;
|
524
|
|
|
|
|
|
|
my $next_seq = FastaReader($file);
|
525
|
|
|
|
|
|
|
while ( my $fa = &$next_seq() ) {
|
526
|
|
|
|
|
|
|
my ( $header, $seq ) = @$fa;
|
527
|
|
|
|
|
|
|
# print ">$header\n$seq\n";
|
528
|
|
|
|
|
|
|
}
|
529
|
|
|
|
|
|
|
};
|
530
|
|
|
|
|
|
|
|
531
|
|
|
|
|
|
|
my ($mean, $stdev) = run_time( 8, $read_by_record, $file );
|
532
|
|
|
|
|
|
|
printf STDERR "\n## Compute time: %0.03f ± %0.03f s\n\n", $mean, $stdev;
|
533
|
|
|
|
|
|
|
|
534
|
|
|
|
|
|
|
=cut
|
535
|
|
|
|
|
|
|
|
536
|
|
|
|
|
|
|
sub run_time {
|
537
|
0
|
|
|
0
|
1
|
|
my ( $n, $sub, @args ) = @_;
|
538
|
0
|
0
|
0
|
|
|
|
die "first argument should be positive integer"
|
539
|
|
|
|
|
|
|
unless $n =~ /^\d+$/ and $n > 0;
|
540
|
|
|
|
|
|
|
|
541
|
0
|
|
|
|
|
|
my $t0 = 0;
|
542
|
0
|
|
|
|
|
|
my @ts = ();
|
543
|
0
|
|
|
|
|
|
for ( 1 .. $n ) {
|
544
|
0
|
|
|
|
|
|
$t0 = time;
|
545
|
0
|
|
|
|
|
|
&$sub(@args); # call $sub
|
546
|
0
|
|
|
|
|
|
push @ts, time - $t0;
|
547
|
|
|
|
|
|
|
}
|
548
|
|
|
|
|
|
|
|
549
|
0
|
|
|
|
|
|
return mean_and_stdev( \@ts );
|
550
|
|
|
|
|
|
|
}
|
551
|
|
|
|
|
|
|
|
552
|
|
|
|
|
|
|
=head2 readable_second
|
553
|
|
|
|
|
|
|
|
554
|
|
|
|
|
|
|
readable_second
|
555
|
|
|
|
|
|
|
|
556
|
|
|
|
|
|
|
Example:
|
557
|
|
|
|
|
|
|
|
558
|
|
|
|
|
|
|
print readable_second(11312314),"\n"; # 130 day 22 hour 18 min 34 sec
|
559
|
|
|
|
|
|
|
|
560
|
|
|
|
|
|
|
=cut
|
561
|
|
|
|
|
|
|
|
562
|
|
|
|
|
|
|
sub readable_second ($) {
|
563
|
0
|
|
|
0
|
1
|
|
my ($seconds) = @_;
|
564
|
0
|
0
|
|
|
|
|
return "Positive integer need." unless $seconds =~ /^\d+$/;
|
565
|
|
|
|
|
|
|
|
566
|
0
|
|
|
|
|
|
my $time = "";
|
567
|
0
|
|
|
|
|
|
my $has_bigger_unit = 0;
|
568
|
|
|
|
|
|
|
|
569
|
0
|
|
|
|
|
|
my $days = $seconds / 86400;
|
570
|
0
|
0
|
|
|
|
|
if ( $days >= 1 ) {
|
571
|
0
|
|
|
|
|
|
$time .= ( int $days ) . " day ";
|
572
|
0
|
|
|
|
|
|
$has_bigger_unit = 1;
|
573
|
|
|
|
|
|
|
}
|
574
|
0
|
|
|
|
|
|
$seconds = $seconds % 86400;
|
575
|
|
|
|
|
|
|
|
576
|
0
|
|
|
|
|
|
my $hours = $seconds / 3600;
|
577
|
0
|
0
|
|
|
|
|
if ( $hours >= 1 ) {
|
|
|
0
|
|
|
|
|
|
578
|
0
|
|
|
|
|
|
$time .= ( int $hours ) . " hour ";
|
579
|
0
|
|
|
|
|
|
$has_bigger_unit = 1;
|
580
|
|
|
|
|
|
|
}
|
581
|
0
|
|
|
|
|
|
elsif ($has_bigger_unit) { $time .= 0 . " hour "; }
|
582
|
0
|
|
|
|
|
|
$seconds = $seconds % 3600;
|
583
|
|
|
|
|
|
|
|
584
|
0
|
|
|
|
|
|
my $minutes = $seconds / 60;
|
585
|
0
|
0
|
|
|
|
|
if ( $minutes >= 1 ) {
|
|
|
0
|
|
|
|
|
|
586
|
0
|
|
|
|
|
|
$time .= ( int $minutes ) . " min ";
|
587
|
0
|
|
|
|
|
|
$has_bigger_unit = 1;
|
588
|
|
|
|
|
|
|
}
|
589
|
0
|
|
|
|
|
|
elsif ($has_bigger_unit) { $time .= 0 . " min "; }
|
590
|
0
|
|
|
|
|
|
$seconds = $seconds % 60;
|
591
|
|
|
|
|
|
|
|
592
|
0
|
|
|
|
|
|
$time .= $seconds . " sec";
|
593
|
0
|
|
|
|
|
|
return $time;
|
594
|
|
|
|
|
|
|
}
|
595
|
|
|
|
|
|
|
|
596
|
|
|
|
|
|
|
=head2 check_positive_integer
|
597
|
|
|
|
|
|
|
|
598
|
|
|
|
|
|
|
Check Positive Integer
|
599
|
|
|
|
|
|
|
|
600
|
|
|
|
|
|
|
Example:
|
601
|
|
|
|
|
|
|
|
602
|
|
|
|
|
|
|
check_positive_integer(1);
|
603
|
|
|
|
|
|
|
|
604
|
|
|
|
|
|
|
=cut
|
605
|
|
|
|
|
|
|
|
606
|
|
|
|
|
|
|
sub check_positive_integer {
|
607
|
0
|
|
|
0
|
1
|
|
my ($n) = @_;
|
608
|
0
|
0
|
0
|
|
|
|
die "positive integer needed ($n given)"
|
609
|
|
|
|
|
|
|
unless $n =~ /^\d+$/ and $n != 0;
|
610
|
|
|
|
|
|
|
}
|
611
|
|
|
|
|
|
|
|
612
|
|
|
|
|
|
|
=head2 mean_and_stdev
|
613
|
|
|
|
|
|
|
|
614
|
|
|
|
|
|
|
return mean and stdev of a list
|
615
|
|
|
|
|
|
|
|
616
|
|
|
|
|
|
|
Example:
|
617
|
|
|
|
|
|
|
my @list = qq/1 2 3/;
|
618
|
|
|
|
|
|
|
mean_and_stdev(\@list);
|
619
|
|
|
|
|
|
|
|
620
|
|
|
|
|
|
|
=cut
|
621
|
|
|
|
|
|
|
sub mean_and_stdev($) {
|
622
|
0
|
|
|
0
|
1
|
|
my ($list) = @_;
|
623
|
0
|
0
|
|
|
|
|
return ( 0, 0 ) if @$list == 0;
|
624
|
0
|
|
|
|
|
|
my $sum = 0;
|
625
|
0
|
|
|
|
|
|
$sum += $_ for @$list;
|
626
|
0
|
|
|
|
|
|
my $sum_square = 0;
|
627
|
0
|
|
|
|
|
|
$sum_square += $_ * $_ for @$list;
|
628
|
0
|
|
|
|
|
|
my $mean = $sum / @$list;
|
629
|
0
|
|
|
|
|
|
my $variance = $sum_square / @$list - $mean * $mean;
|
630
|
0
|
|
|
|
|
|
my $std = sqrt $variance;
|
631
|
0
|
|
|
|
|
|
return ( $mean, $std );
|
632
|
|
|
|
|
|
|
}
|
633
|
|
|
|
|
|
|
|
634
|
|
|
|
|
|
|
=head2 filename_prefix
|
635
|
|
|
|
|
|
|
|
636
|
|
|
|
|
|
|
Get filename prefix
|
637
|
|
|
|
|
|
|
|
638
|
|
|
|
|
|
|
Example:
|
639
|
|
|
|
|
|
|
|
640
|
|
|
|
|
|
|
filename_prefix("test.fa"); # "test"
|
641
|
|
|
|
|
|
|
filename_prefix("tmp"); # "tmp"
|
642
|
|
|
|
|
|
|
|
643
|
|
|
|
|
|
|
=cut
|
644
|
|
|
|
|
|
|
|
645
|
|
|
|
|
|
|
sub filename_prefix {
|
646
|
0
|
|
|
0
|
1
|
|
my ($file) = @_;
|
647
|
0
|
0
|
|
|
|
|
if ( $file =~ /(.+)\..+?$/ ) {
|
648
|
0
|
|
|
|
|
|
return $1;
|
649
|
|
|
|
|
|
|
}
|
650
|
|
|
|
|
|
|
else {
|
651
|
0
|
|
|
|
|
|
return $file;
|
652
|
|
|
|
|
|
|
}
|
653
|
|
|
|
|
|
|
}
|
654
|
|
|
|
|
|
|
|
655
|
|
|
|
|
|
|
=head2 check_all_files_exist
|
656
|
|
|
|
|
|
|
|
657
|
|
|
|
|
|
|
Check whether all files existed.
|
658
|
|
|
|
|
|
|
|
659
|
|
|
|
|
|
|
=cut
|
660
|
|
|
|
|
|
|
|
661
|
|
|
|
|
|
|
sub check_all_files_exist {
|
662
|
0
|
|
|
0
|
1
|
|
my $flag = 1;
|
663
|
0
|
|
|
|
|
|
for (@_) {
|
664
|
0
|
0
|
|
|
|
|
if ( not -e $_ ) {
|
665
|
0
|
|
|
|
|
|
return 0;
|
666
|
|
|
|
|
|
|
}
|
667
|
|
|
|
|
|
|
}
|
668
|
0
|
|
|
|
|
|
return 1;
|
669
|
|
|
|
|
|
|
}
|
670
|
|
|
|
|
|
|
|
671
|
|
|
|
|
|
|
=head2 check_in_out_dir
|
672
|
|
|
|
|
|
|
|
673
|
|
|
|
|
|
|
Check in and $fh2 directory.
|
674
|
|
|
|
|
|
|
|
675
|
|
|
|
|
|
|
Example:
|
676
|
|
|
|
|
|
|
|
677
|
|
|
|
|
|
|
check_in_out_dir("~/dir", "~/dir.out");
|
678
|
|
|
|
|
|
|
|
679
|
|
|
|
|
|
|
=cut
|
680
|
|
|
|
|
|
|
|
681
|
|
|
|
|
|
|
sub check_in_out_dir {
|
682
|
0
|
|
|
0
|
1
|
|
my ( $in, $out ) = @_;
|
683
|
0
|
0
|
|
|
|
|
die "dir $in not found."
|
684
|
|
|
|
|
|
|
unless -e $in;
|
685
|
|
|
|
|
|
|
|
686
|
0
|
0
|
|
|
|
|
die "$in is not a directory.\n"
|
687
|
|
|
|
|
|
|
unless -d $in;
|
688
|
|
|
|
|
|
|
|
689
|
0
|
|
|
|
|
|
$in =~ s/\/$//;
|
690
|
0
|
|
|
|
|
|
$out =~ s/\/$//;
|
691
|
0
|
0
|
|
|
|
|
die "out dir shoud be different from in dir!\n"
|
692
|
|
|
|
|
|
|
if $in eq $out;
|
693
|
|
|
|
|
|
|
}
|
694
|
|
|
|
|
|
|
|
695
|
|
|
|
|
|
|
=head2 rm_and_mkdir
|
696
|
|
|
|
|
|
|
|
697
|
|
|
|
|
|
|
Make a directory, remove it firstly if it exists.
|
698
|
|
|
|
|
|
|
|
699
|
|
|
|
|
|
|
Example:
|
700
|
|
|
|
|
|
|
|
701
|
|
|
|
|
|
|
rm_and_mkdir("out")
|
702
|
|
|
|
|
|
|
|
703
|
|
|
|
|
|
|
=cut
|
704
|
|
|
|
|
|
|
|
705
|
|
|
|
|
|
|
sub rm_and_mkdir {
|
706
|
0
|
|
|
0
|
1
|
|
my ($dir) = @_;
|
707
|
0
|
0
|
|
|
|
|
if ( -e $dir ) {
|
708
|
0
|
0
|
|
|
|
|
remove_tree($dir) or die "fail to remove: $dir\n";
|
709
|
|
|
|
|
|
|
}
|
710
|
0
|
0
|
|
|
|
|
mkdir $dir or die "fail to mkdir: $dir\n";
|
711
|
|
|
|
|
|
|
}
|
712
|
|
|
|
|
|
|
|
713
|
|
|
|
|
|
|
=head2 get_paired_fq_gz_file_from_dir
|
714
|
|
|
|
|
|
|
|
715
|
|
|
|
|
|
|
Example:
|
716
|
|
|
|
|
|
|
|
717
|
|
|
|
|
|
|
# .
|
718
|
|
|
|
|
|
|
# ├── test_1.fq.gz
|
719
|
|
|
|
|
|
|
# └── test_2.fq.gz
|
720
|
|
|
|
|
|
|
for my $pe ( get_paired_fq_gz_file_from_dir($indir) ) {
|
721
|
|
|
|
|
|
|
# test_1.fq.gz, test_1.fq.gz, test
|
722
|
|
|
|
|
|
|
my ( $fqfile1, $fqfile2, $id ) = @$pe;
|
723
|
|
|
|
|
|
|
|
724
|
|
|
|
|
|
|
}
|
725
|
|
|
|
|
|
|
|
726
|
|
|
|
|
|
|
=cut
|
727
|
|
|
|
|
|
|
|
728
|
|
|
|
|
|
|
sub get_paired_fq_gz_file_from_dir {
|
729
|
0
|
|
|
0
|
1
|
|
my ($dir) = @_;
|
730
|
0
|
|
|
|
|
|
my @files;
|
731
|
0
|
|
|
|
|
|
for ( sort glob "$dir/*_1.fq.gz" ) {
|
732
|
0
|
|
|
|
|
|
/\/?([^\/]+)_1.fq.gz/;
|
733
|
0
|
|
|
|
|
|
my $id = $1;
|
734
|
0
|
0
|
|
|
|
|
next unless -e "$dir/${id}_2.fq.gz";
|
735
|
0
|
|
|
|
|
|
push @files, [ "$dir/${id}_1.fq.gz", "$dir/${id}_2.fq.gz", "$dir/${id}", $id ];
|
736
|
|
|
|
|
|
|
}
|
737
|
0
|
|
|
|
|
|
return @files;
|
738
|
|
|
|
|
|
|
}
|
739
|
|
|
|
|
|
|
|
740
|
|
|
|
|
|
|
sub get_paired_fa_gz_file_from_dir {
|
741
|
0
|
|
|
0
|
0
|
|
my ($dir) = @_;
|
742
|
0
|
|
|
|
|
|
my @files;
|
743
|
0
|
|
|
|
|
|
for ( sort glob "$dir/*_1.fa.gz" ) {
|
744
|
0
|
|
|
|
|
|
/\/?([^\/]+)_1.fa.gz/;
|
745
|
0
|
|
|
|
|
|
my $id = $1;
|
746
|
0
|
0
|
|
|
|
|
next unless -e "$dir/${id}_2.fa.gz";
|
747
|
0
|
|
|
|
|
|
push @files, [ "$dir/${id}_1.fa.gz", "$dir/${id}_2.fa.gz", "$dir/${id}", $id ];
|
748
|
|
|
|
|
|
|
}
|
749
|
0
|
|
|
|
|
|
return @files;
|
750
|
|
|
|
|
|
|
}
|
751
|
|
|
|
|
|
|
|
752
|
|
|
|
|
|
|
1;
|