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# $Id: Config.pm kortsch $ |
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# |
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# BioPerl module for Bio::Tools::Run::BEDTools::Config |
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# |
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# Please direct questions and support issues to |
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# |
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# Cared for by Dan Kortschak |
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# |
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# Copyright Dan Kortschak |
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# |
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# You may distribute this module under the same terms as perl itself |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::Tools::Run::BEDTools::Config - Configuration data for bowtie commands |
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=head1 SYNOPSIS |
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Used internally by L. |
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=head1 DESCRIPTION |
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This package exports information describing BEDTools commands, parameters, |
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switches, and input and output filetypes for individual BEDTools commands. |
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=head1 FEEDBACK |
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=head2 Mailing Lists |
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User feedback is an integral part of the evolution of this and other |
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Bioperl modules. Send your comments and suggestions preferably to |
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the Bioperl mailing list. Your participation is much appreciated. |
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bioperl-l@bioperl.org - General discussion |
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http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
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=head2 Support |
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Please direct usage questions or support issues to the mailing list: |
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L |
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rather than to the module maintainer directly. Many experienced and |
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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=head2 Reporting Bugs |
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Report bugs to the Bioperl bug tracking system to help us keep track |
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of the bugs and their resolution. Bug reports can be submitted via |
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the web: |
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http://redmine.open-bio.org/projects/bioperl/ |
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=head1 AUTHOR - Dan Kortschak |
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Email dan.kortschak adelaide.edu.au |
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=head1 CONTRIBUTORS |
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Additional contributors names and emails here |
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=head1 APPENDIX |
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The rest of the documentation details each of the object methods. |
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Internal methods are usually preceded with a _ |
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=cut |
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# Let the code begin... |
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package Bio::Tools::Run::BEDTools::Config; |
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use strict; |
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use warnings; |
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no warnings qw(qw); |
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use Bio::Root::Root; |
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use Exporter; |
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use base qw(Bio::Root::Root); |
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our (@ISA, @EXPORT, @EXPORT_OK); |
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push @ISA, 'Exporter'; |
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@EXPORT = qw( |
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@program_commands |
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%command_executables |
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%format_lookup |
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%command_prefixes |
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%composite_commands |
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@program_params |
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@program_switches |
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%param_translation |
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%command_files |
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%accepted_types |
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); |
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@EXPORT_OK = qw(); |
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our @program_commands = qw( |
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annotate fasta_from_bed overlap |
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bam_to_bed genome_coverage pair_to_pair |
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bed_to_bam graph_union pair_to_bed |
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bed_to_IGV group_by shuffle |
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b12_to_b6 intersect slop |
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closest links sort |
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complement mask_fasta_from_bed subtract |
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coverage merge window |
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); |
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our %command_executables = ( |
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'annotate' => 'annotateBed', |
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'bam_to_bed' => 'bamToBed', |
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'bed_to_bam' => 'bedToBam', |
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'bed_to_IGV' => 'bedToIgv', |
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'b12_to_b6' => 'bed12ToBed6', |
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'fasta_from_bed' => 'fastaFromBed', |
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'mask_fasta_from_bed' => 'maskFastaFromBed', |
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'shuffle' => 'shuffleBed', |
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'window' => 'windowBed', |
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'closest' => 'closestBed', |
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'genome_coverage' => 'genomeCoverageBed', |
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'merge' => 'mergeBed', |
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'slop' => 'slopBed', |
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'complement' => 'complementBed', |
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'intersect' => 'intersectBed', |
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'pair_to_bed' => 'pairToBed', |
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'sort' => 'sortBed', |
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'coverage' => 'coverageBed', |
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'links' => 'linksBed', |
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'pair_to_pair' => 'pairToPair', |
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'subtract' => 'subtractBed', |
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'overlap' => 'overlap', |
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'group_by' => 'groupBy', |
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'graph_union' => 'unionBedGraphs' |
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); |
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our %format_lookup = ( |
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'annotate' => 'bed', |
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'bam_to_bed' => 'bed', |
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'bed_to_bam' => 'bam', |
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'bed_to_IGV' => 'igv', |
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'b12_to_b6' => 'bed', |
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'closest' => 'bedpe', |
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'complement' => 'bed', |
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'coverage' => 'bed', |
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'fasta_from_bed' => 'fasta', |
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'genome_coverage' => 'tab', |
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'graph_union' => 'bg', |
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'group_by' => 'bed', |
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'intersect' => 'bed|bam', |
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'links' => 'html', |
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'mask_fasta_from_bed' => 'fasta', |
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'merge' => 'bed', |
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'overlap' => 'bed', |
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'pair_to_bed' => 'bedpe|bam', |
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'pair_to_pair' => 'bedpe', |
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'slop' => 'bed', |
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'shuffle' => 'bed', |
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'sort' => 'bed', |
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'subtract' => 'bed', |
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'window' => 'bedpe' |
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); |
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# composite commands: pseudo-commands that run a |
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# sequence of commands |
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# composite command prefix => list of prefixes of commands this |
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# composite command runs |
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# |
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our %composite_commands = ( |
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); |
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# prefixes only for commands that take params/switches... |
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our %command_prefixes = ( |
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'annotate' => 'ann', |
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'bam_to_bed' => 'ate', |
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'bed_to_bam' => 'eta', |
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'bed_to_IGV' => 'eti', |
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'b12_to_b6' => '126', |
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'fasta_from_bed' => 'ffb', |
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'mask_fasta_from_bed' => 'mfb', |
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'shuffle' => 'shb', |
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'window' => 'wib', |
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'closest' => 'clb', |
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'genome_coverage' => 'gcb', |
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'merge' => 'meb', |
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'slop' => 'slb', |
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'complement' => 'cob', |
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'intersect' => 'inb', |
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'pair_to_bed' => 'ptb', |
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'sort' => 'sob', |
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'coverage' => 'cvb', |
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'links' => 'lib', |
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'pair_to_pair' => 'ptp', |
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'subtract' => 'sub', |
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'overlap' => 'ove', |
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'group_by' => 'grp', |
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'graph_union' => 'ubg' |
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); |
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our @program_params = qw( |
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command |
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ate|tag |
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ate|color |
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eta|quality |
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eti|path |
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eti|session |
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eti|sort |
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eti|slop |
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eti|image |
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shb|exclude |
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shb|seed |
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wib|window_size |
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wib|left_window_size |
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wib|right_window_size |
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clb|ties_policy |
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gcb|max_depth |
231
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gcb|strand |
232
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233
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meb|max_distance |
234
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235
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slb|add_bidirectional |
236
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slb|add_to_left |
237
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slb|add_to_right |
238
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239
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inb|minimum_overlap |
240
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241
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ptb|minimum_overlap |
242
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ptb|type |
243
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244
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ptp|minimum_overlap |
245
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ptp|type |
246
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ptp|slop |
247
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248
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sub|minimum_overlap |
249
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250
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lib|basename |
251
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lib|organism |
252
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lib|genome_build |
253
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254
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ove|columns |
255
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256
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grp|group |
257
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grp|columns |
258
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grp|operations |
259
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260
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ubg|names |
261
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ubg|filler |
262
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); |
263
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264
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our @program_switches = qw( |
265
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ann|names |
266
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ann|count |
267
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ann|both |
268
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ann|strandedness |
269
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270
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ate|write_bedpe |
271
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ate|use_edit_distance |
272
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ate|bam12 |
273
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ate|split |
274
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ate|use_edit_distance |
275
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ate|cigar |
276
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277
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eta|uncompressed |
278
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eta|bed12 |
279
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280
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eti|collapse |
281
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eti|name |
282
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283
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ffb|use_bed_name |
284
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ffb|output_tab_format |
285
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ffb|strandedness |
286
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287
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gcb|bedgraph |
288
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gcb|bedgraph_all |
289
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gcb|split |
290
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291
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mfb|soft_mask |
292
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293
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shb|keep_chromosome |
294
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295
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wib|define_by_strand |
296
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wib|same_strand |
297
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wib|report_once_only |
298
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wib|report_hits |
299
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wib|invert |
300
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301
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clb|strandedness |
302
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clb|report_distance |
303
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304
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gcb|report_pos_depth |
305
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306
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meb|strandedness |
307
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meb|report_n_merged |
308
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meb|report_names_merged |
309
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310
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slb|define_by_strand |
311
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312
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inb|write_bed |
313
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inb|write_entry_1 |
314
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inb|write_entry_2 |
315
|
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inb|report_once_only |
316
|
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inb|report_n_hits |
317
|
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inb|invert_match |
318
|
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inb|reciprocal |
319
|
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inb|strandedness |
320
|
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inb|write_overlap |
321
|
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inb|write_overlap_all |
322
|
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inb|split |
323
|
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|
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324
|
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ptb|write_bedpe |
325
|
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ptb|strandedness |
326
|
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|
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|
ptb|use_edit_distance |
327
|
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ptb|write_uncompressed |
328
|
|
|
|
|
|
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329
|
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|
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|
sob|size_asc |
330
|
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sob|size_desc |
331
|
|
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|
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sob|chr_size_asc |
332
|
|
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|
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sob|chr_size_desc |
333
|
|
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|
|
|
|
sob|chr_score_asc |
334
|
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sob|chr_score_desc |
335
|
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|
|
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336
|
|
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|
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cvb|strandedness |
337
|
|
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|
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|
cvb|histogram |
338
|
|
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|
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|
cvb|depth |
339
|
|
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|
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cvb|split |
340
|
|
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|
|
|
|
|
341
|
|
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|
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|
ptp|ignore_strand |
342
|
|
|
|
|
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|
ptp|slop_strandedness |
343
|
|
|
|
|
|
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ptp|no_self_hits |
344
|
|
|
|
|
|
|
|
345
|
|
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|
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|
sub|strandedness |
346
|
|
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|
|
|
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|
347
|
|
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|
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|
|
ubg|header |
348
|
|
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|
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|
ubg|empty |
349
|
|
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|
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|
); |
350
|
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|
351
|
|
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|
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|
|
our %param_translation = ( |
352
|
|
|
|
|
|
|
'ann|names' => 'names', |
353
|
|
|
|
|
|
|
'ann|counts' => 'counts', |
354
|
|
|
|
|
|
|
'ann|both' => 'both', |
355
|
|
|
|
|
|
|
'ann|strandedness' => 's', |
356
|
|
|
|
|
|
|
|
357
|
|
|
|
|
|
|
'ate|write_bedpe' => 'bedpe', |
358
|
|
|
|
|
|
|
'ate|use_edit_distance' => 'ed', |
359
|
|
|
|
|
|
|
'ate|bam12' => 'bam12', |
360
|
|
|
|
|
|
|
'ate|split' => 'split', |
361
|
|
|
|
|
|
|
'ate|use_edit_distance' => 'ed', |
362
|
|
|
|
|
|
|
'ate|tag' => 'tag', |
363
|
|
|
|
|
|
|
'ate|color' => 'color', |
364
|
|
|
|
|
|
|
'ate|cigar' => 'cigar', |
365
|
|
|
|
|
|
|
|
366
|
|
|
|
|
|
|
'eta|quality' => 'maqp', |
367
|
|
|
|
|
|
|
'eta|uncompressed' => 'ubam', |
368
|
|
|
|
|
|
|
'eta|bed12' => 'bed12', |
369
|
|
|
|
|
|
|
|
370
|
|
|
|
|
|
|
'eti|path' => 'path', |
371
|
|
|
|
|
|
|
'eti|session' => 'sess', |
372
|
|
|
|
|
|
|
'eti|sort' => 'sort', |
373
|
|
|
|
|
|
|
'eti|collapse' => 'clps', |
374
|
|
|
|
|
|
|
'eti|name' => 'name', |
375
|
|
|
|
|
|
|
'eti|slop' => 'slop', |
376
|
|
|
|
|
|
|
'eti|image' => 'img', |
377
|
|
|
|
|
|
|
|
378
|
|
|
|
|
|
|
'ffb|use_bed_name' => 'names', |
379
|
|
|
|
|
|
|
'ffb|output_tab_format' => 'tab', |
380
|
|
|
|
|
|
|
'ffb|strandedness' => 's', |
381
|
|
|
|
|
|
|
|
382
|
|
|
|
|
|
|
'mfb|soft_mask' => 'soft', |
383
|
|
|
|
|
|
|
|
384
|
|
|
|
|
|
|
'shb|keep_chromosome' => 'chrom', |
385
|
|
|
|
|
|
|
'shb|exclude' => 'excl', |
386
|
|
|
|
|
|
|
'shb|seed' => 'seed', |
387
|
|
|
|
|
|
|
|
388
|
|
|
|
|
|
|
'wib|define_by_strand' => 'sw', |
389
|
|
|
|
|
|
|
'wib|same_strand' => 'sm', |
390
|
|
|
|
|
|
|
'wib|report_once_only' => 'u', |
391
|
|
|
|
|
|
|
'wib|report_hits' => 'c', |
392
|
|
|
|
|
|
|
'wib|invert' => 'v', |
393
|
|
|
|
|
|
|
'wib|window_size' => 'w', |
394
|
|
|
|
|
|
|
'wib|left_window_size' => 'l', |
395
|
|
|
|
|
|
|
'wib|right_window_size' => 'r', |
396
|
|
|
|
|
|
|
|
397
|
|
|
|
|
|
|
'clb|strandedness' => 's', |
398
|
|
|
|
|
|
|
'clb|report_distance' => 'd', |
399
|
|
|
|
|
|
|
'clb|ties_policy' => 't', |
400
|
|
|
|
|
|
|
|
401
|
|
|
|
|
|
|
'gcb|report_pos_depth' => 'd', |
402
|
|
|
|
|
|
|
'gcb|max_depth' => 'max', |
403
|
|
|
|
|
|
|
'gcb|bedgraph' => 'bg', |
404
|
|
|
|
|
|
|
'gcb|bedgraph_all' => 'bga', |
405
|
|
|
|
|
|
|
'gcb|split' => 'split', |
406
|
|
|
|
|
|
|
'gcb|strand' => 'strand', |
407
|
|
|
|
|
|
|
|
408
|
|
|
|
|
|
|
'meb|strandedness' => 's', |
409
|
|
|
|
|
|
|
'meb|report_n_merged' => 'n', |
410
|
|
|
|
|
|
|
'meb|report_names_merged' => 'nms', |
411
|
|
|
|
|
|
|
'meb|max_distance' => 'd', |
412
|
|
|
|
|
|
|
|
413
|
|
|
|
|
|
|
'slb|define_by_strand' => 's', |
414
|
|
|
|
|
|
|
'slb|add_bidirectional' => 'b', |
415
|
|
|
|
|
|
|
'slb|add_to_left' => 'l', |
416
|
|
|
|
|
|
|
'slb|add_to_right' => 'r', |
417
|
|
|
|
|
|
|
|
418
|
|
|
|
|
|
|
'inb|write_bed' => 'bed', |
419
|
|
|
|
|
|
|
'inb|write_entry_1' => 'wa', |
420
|
|
|
|
|
|
|
'inb|write_entry_2' => 'wb', |
421
|
|
|
|
|
|
|
'inb|write_overlap' => 'wo', |
422
|
|
|
|
|
|
|
'inb|write_overlap_all' => 'woa', |
423
|
|
|
|
|
|
|
'inb|report_once_only' => 'u', |
424
|
|
|
|
|
|
|
'inb|report_n_hits' => 'c', |
425
|
|
|
|
|
|
|
'inb|invert_match' => 'v', |
426
|
|
|
|
|
|
|
'inb|reciprocal' => 'r', |
427
|
|
|
|
|
|
|
'inb|strandedness' => 's', |
428
|
|
|
|
|
|
|
'inb|minimum_overlap' => 'f', |
429
|
|
|
|
|
|
|
'inb|split' => 'split', |
430
|
|
|
|
|
|
|
|
431
|
|
|
|
|
|
|
'ptb|write_bedpe' => 'bedpe', |
432
|
|
|
|
|
|
|
'ptb|strandedness' => 's', |
433
|
|
|
|
|
|
|
'ptb|minimum_overlap' => 'f', |
434
|
|
|
|
|
|
|
'ptb|type' => 'type', |
435
|
|
|
|
|
|
|
'ptb|use_edit_distance' => 'ed', |
436
|
|
|
|
|
|
|
'ptb|write_uncompressed' => 'ubam', |
437
|
|
|
|
|
|
|
|
438
|
|
|
|
|
|
|
'sob|size_asc' => 'sizeA', |
439
|
|
|
|
|
|
|
'sob|size_desc' => 'sizeD', |
440
|
|
|
|
|
|
|
'sob|chr_size_asc' => 'chrThenSizeA', |
441
|
|
|
|
|
|
|
'sob|chr_size_desc' => 'chrThenSizeD', |
442
|
|
|
|
|
|
|
'sob|chr_score_asc' => 'chrThenScoreA', |
443
|
|
|
|
|
|
|
'sob|chr_score_desc' => 'chrThenScoreD', |
444
|
|
|
|
|
|
|
|
445
|
|
|
|
|
|
|
'cvb|strandedness' => 's', |
446
|
|
|
|
|
|
|
'cvb|histogram' => 'hist', |
447
|
|
|
|
|
|
|
'cvb|depth' => 'd', |
448
|
|
|
|
|
|
|
'cvb|split' => 'split', |
449
|
|
|
|
|
|
|
|
450
|
|
|
|
|
|
|
'ptp|ignore_strand' => 'is', |
451
|
|
|
|
|
|
|
'ptp|slop_strandedness' => 'ss', |
452
|
|
|
|
|
|
|
'ptp|minimum_overlap' => 'f', |
453
|
|
|
|
|
|
|
'ptp|type' => 'type', |
454
|
|
|
|
|
|
|
'ptp|slop' => 'slop', |
455
|
|
|
|
|
|
|
'ptp|no_self_hits' => 'rdn', |
456
|
|
|
|
|
|
|
|
457
|
|
|
|
|
|
|
'sub|strandedness' => 's', |
458
|
|
|
|
|
|
|
'sub|minimum_overlap' => 'f', |
459
|
|
|
|
|
|
|
|
460
|
|
|
|
|
|
|
'lib|basename' => 'base', |
461
|
|
|
|
|
|
|
'lib|organism' => 'org', |
462
|
|
|
|
|
|
|
'lib|genome_build' => 'db', |
463
|
|
|
|
|
|
|
|
464
|
|
|
|
|
|
|
'ove|columns' => 'cols', |
465
|
|
|
|
|
|
|
|
466
|
|
|
|
|
|
|
'grp|group' => 'grp', |
467
|
|
|
|
|
|
|
'grp|columns' => 'opCols', |
468
|
|
|
|
|
|
|
'grp|operations' => 'ops', |
469
|
|
|
|
|
|
|
|
470
|
|
|
|
|
|
|
'ubg|header' => 'header', |
471
|
|
|
|
|
|
|
'ubg|names' => 'names', |
472
|
|
|
|
|
|
|
'ubg|empty' => 'empty', |
473
|
|
|
|
|
|
|
'ubg|filler' => 'filler' |
474
|
|
|
|
|
|
|
); |
475
|
|
|
|
|
|
|
|
476
|
|
|
|
|
|
|
# |
477
|
|
|
|
|
|
|
# the order in the arrayrefs is the order required |
478
|
|
|
|
|
|
|
# on the command line |
479
|
|
|
|
|
|
|
# |
480
|
|
|
|
|
|
|
# the strings in the arrayrefs (less special chars) |
481
|
|
|
|
|
|
|
# become the keys for named parameters to run_bowtie |
482
|
|
|
|
|
|
|
# |
483
|
|
|
|
|
|
|
# special chars: |
484
|
|
|
|
|
|
|
# |
485
|
|
|
|
|
|
|
# '#' implies optional |
486
|
|
|
|
|
|
|
# '*' implies variable number of this type |
487
|
|
|
|
|
|
|
# <|> implies stdin/stdout redirect |
488
|
|
|
|
|
|
|
# |
489
|
|
|
|
|
|
|
|
490
|
|
|
|
|
|
|
our %command_files = ( |
491
|
|
|
|
|
|
|
'annotate' => [qw( -i|bgv -files|*ann >#out )], |
492
|
|
|
|
|
|
|
'bam_to_bed' => [qw( -i|bam >#out )], |
493
|
|
|
|
|
|
|
'bed_to_bam' => [qw( -i|bgv -g|genome >#out )], |
494
|
|
|
|
|
|
|
'bed_to_IGV' => [qw( -i|bgv >#out )], |
495
|
|
|
|
|
|
|
'b12_to_b6' => [qw( -i|bed >#out )], |
496
|
|
|
|
|
|
|
'fasta_from_bed' => [qw( -fi|seq -bed|bgv -fo|#out )], |
497
|
|
|
|
|
|
|
'mask_fasta_from_bed' => [qw( -fi|seq -bed|bgv -fo|#out )], |
498
|
|
|
|
|
|
|
'shuffle' => [qw( -i|bgv -g|genome >#out )], |
499
|
|
|
|
|
|
|
'window' => [qw( -a|bgv1 -b|bgv2 >#out )], |
500
|
|
|
|
|
|
|
'closest' => [qw( -a|bgv1 -b|bgv2 >#out )], |
501
|
|
|
|
|
|
|
'genome_coverage' => [qw( -i|bed -g|genome >#out )], |
502
|
|
|
|
|
|
|
'merge' => [qw( -i|bgv >#out )], |
503
|
|
|
|
|
|
|
'slop' => [qw( -i|bgv -g|genome >#out )], |
504
|
|
|
|
|
|
|
'complement' => [qw( -i|bgv -g|genome >#out )], |
505
|
|
|
|
|
|
|
'intersect' => [qw( -a|#bgv1 -abam|#bam -b|bgv2 >#out )], # (bgv1|bam) required |
506
|
|
|
|
|
|
|
'pair_to_bed' => [qw( -a|#bedpe -abam|#bam -b|bgv >#out )], # (bedpe|bam) required |
507
|
|
|
|
|
|
|
'sort' => [qw( -i|bgv >#out )], |
508
|
|
|
|
|
|
|
'coverage' => [qw( -a|bgv1 -b|bgv2 >#out )], |
509
|
|
|
|
|
|
|
'links' => [qw( -i|bgv >#out )], |
510
|
|
|
|
|
|
|
'pair_to_pair' => [qw( -a|bedpe1 -b|bedpe2 >#out )], |
511
|
|
|
|
|
|
|
'subtract' => [qw( -a|bgv1 -b|bgv2 >#out )], |
512
|
|
|
|
|
|
|
'group_by' => [qw( -i|bed >#out )], |
513
|
|
|
|
|
|
|
'graph_union' => [qw( -i|*bg -g|#genome >#out )], |
514
|
|
|
|
|
|
|
'overlap' => [qw( -i|bed >#out )] |
515
|
|
|
|
|
|
|
); |
516
|
|
|
|
|
|
|
|
517
|
|
|
|
|
|
|
our %accepted_types = ( |
518
|
|
|
|
|
|
|
'ann' => [qw( tab vcf gff )], # BEDTools now has multiple accepted input formats: bed/gff/vcf |
519
|
|
|
|
|
|
|
'bam' => [qw()], # we need a test for this |
520
|
|
|
|
|
|
|
'bed' => [qw( tab )], |
521
|
|
|
|
|
|
|
'bgv' => [qw( tab vcf gff )], # BEDTools now has multiple accepted input formats: bed/gff/vcf |
522
|
|
|
|
|
|
|
'bgv1' => [qw( tab vcf gff )], # BEDTools now has multiple accepted input formats: bed/gff/vcf |
523
|
|
|
|
|
|
|
'bgv2' => [qw( tab vcf gff )], # BEDTools now has multiple accepted input formats: bed/gff/vcf |
524
|
|
|
|
|
|
|
'bedpe' => [qw( tab )], |
525
|
|
|
|
|
|
|
'bedpe1' => [qw( tab )], |
526
|
|
|
|
|
|
|
'bedpe2' => [qw( tab )], |
527
|
|
|
|
|
|
|
'seq' => [qw( fasta )], |
528
|
|
|
|
|
|
|
'genome' => [qw( tab )], |
529
|
|
|
|
|
|
|
'bg' => [qw( tab )] |
530
|
|
|
|
|
|
|
); |
531
|
|
|
|
|
|
|
|
532
|
|
|
|
|
|
|
1; |