line |
stmt |
bran |
cond |
sub |
pod |
time |
code |
1
|
|
|
|
|
|
|
# $Id$ |
2
|
|
|
|
|
|
|
# |
3
|
|
|
|
|
|
|
# BioPerl module for Bio::Tools::Run::Maq::Config |
4
|
|
|
|
|
|
|
# |
5
|
|
|
|
|
|
|
# Please direct questions and support issues to |
6
|
|
|
|
|
|
|
# |
7
|
|
|
|
|
|
|
# Cared for by Mark A. Jensen |
8
|
|
|
|
|
|
|
# |
9
|
|
|
|
|
|
|
# Copyright Mark A. Jensen |
10
|
|
|
|
|
|
|
# |
11
|
|
|
|
|
|
|
# You may distribute this module under the same terms as perl itself |
12
|
|
|
|
|
|
|
|
13
|
|
|
|
|
|
|
# POD documentation - main docs before the code |
14
|
|
|
|
|
|
|
|
15
|
|
|
|
|
|
|
=head1 NAME |
16
|
|
|
|
|
|
|
|
17
|
|
|
|
|
|
|
Bio::Tools::Run::Maq::Config - Configuration data for maq commands |
18
|
|
|
|
|
|
|
|
19
|
|
|
|
|
|
|
=head1 SYNOPSIS |
20
|
|
|
|
|
|
|
|
21
|
|
|
|
|
|
|
Used internally by L. |
22
|
|
|
|
|
|
|
|
23
|
|
|
|
|
|
|
=head1 DESCRIPTION |
24
|
|
|
|
|
|
|
|
25
|
|
|
|
|
|
|
This package exports information describing maq commands, parameters, |
26
|
|
|
|
|
|
|
switches, and input and output filetypes for individual maq commands. |
27
|
|
|
|
|
|
|
|
28
|
|
|
|
|
|
|
=head1 FEEDBACK |
29
|
|
|
|
|
|
|
|
30
|
|
|
|
|
|
|
=head2 Mailing Lists |
31
|
|
|
|
|
|
|
|
32
|
|
|
|
|
|
|
User feedback is an integral part of the evolution of this and other |
33
|
|
|
|
|
|
|
Bioperl modules. Send your comments and suggestions preferably to |
34
|
|
|
|
|
|
|
the Bioperl mailing list. Your participation is much appreciated. |
35
|
|
|
|
|
|
|
|
36
|
|
|
|
|
|
|
bioperl-l@bioperl.org - General discussion |
37
|
|
|
|
|
|
|
http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
38
|
|
|
|
|
|
|
|
39
|
|
|
|
|
|
|
=head2 Support |
40
|
|
|
|
|
|
|
|
41
|
|
|
|
|
|
|
Please direct usage questions or support issues to the mailing list: |
42
|
|
|
|
|
|
|
|
43
|
|
|
|
|
|
|
L |
44
|
|
|
|
|
|
|
|
45
|
|
|
|
|
|
|
rather than to the module maintainer directly. Many experienced and |
46
|
|
|
|
|
|
|
reponsive experts will be able look at the problem and quickly |
47
|
|
|
|
|
|
|
address it. Please include a thorough description of the problem |
48
|
|
|
|
|
|
|
with code and data examples if at all possible. |
49
|
|
|
|
|
|
|
|
50
|
|
|
|
|
|
|
=head2 Reporting Bugs |
51
|
|
|
|
|
|
|
|
52
|
|
|
|
|
|
|
Report bugs to the Bioperl bug tracking system to help us keep track |
53
|
|
|
|
|
|
|
of the bugs and their resolution. Bug reports can be submitted via |
54
|
|
|
|
|
|
|
the web: |
55
|
|
|
|
|
|
|
|
56
|
|
|
|
|
|
|
http://redmine.open-bio.org/projects/bioperl/ |
57
|
|
|
|
|
|
|
|
58
|
|
|
|
|
|
|
=head1 AUTHOR - Mark A. Jensen |
59
|
|
|
|
|
|
|
|
60
|
|
|
|
|
|
|
Email maj@fortinbras.us |
61
|
|
|
|
|
|
|
|
62
|
|
|
|
|
|
|
Describe contact details here |
63
|
|
|
|
|
|
|
|
64
|
|
|
|
|
|
|
=head1 CONTRIBUTORS |
65
|
|
|
|
|
|
|
|
66
|
|
|
|
|
|
|
Additional contributors names and emails here |
67
|
|
|
|
|
|
|
|
68
|
|
|
|
|
|
|
=head1 APPENDIX |
69
|
|
|
|
|
|
|
|
70
|
|
|
|
|
|
|
The rest of the documentation details each of the object methods. |
71
|
|
|
|
|
|
|
Internal methods are usually preceded with a _ |
72
|
|
|
|
|
|
|
|
73
|
|
|
|
|
|
|
=cut |
74
|
|
|
|
|
|
|
|
75
|
|
|
|
|
|
|
# Let the code begin... |
76
|
|
|
|
|
|
|
|
77
|
|
|
|
|
|
|
|
78
|
|
|
|
|
|
|
package Bio::Tools::Run::Maq::Config; |
79
|
1
|
|
|
1
|
|
4
|
use strict; |
|
1
|
|
|
|
|
1
|
|
|
1
|
|
|
|
|
39
|
|
80
|
1
|
|
|
1
|
|
4
|
use warnings; |
|
1
|
|
|
|
|
1
|
|
|
1
|
|
|
|
|
23
|
|
81
|
1
|
|
|
1
|
|
3
|
no warnings qw(qw); |
|
1
|
|
|
|
|
1
|
|
|
1
|
|
|
|
|
27
|
|
82
|
1
|
|
|
1
|
|
3
|
use Bio::Root::Root; |
|
1
|
|
|
|
|
1
|
|
|
1
|
|
|
|
|
13
|
|
83
|
1
|
|
|
1
|
|
15
|
use Exporter; |
|
1
|
|
|
|
|
1
|
|
|
1
|
|
|
|
|
29
|
|
84
|
1
|
|
|
1
|
|
3
|
use base qw(Bio::Root::Root ); |
|
1
|
|
|
|
|
3
|
|
|
1
|
|
|
|
|
481
|
|
85
|
|
|
|
|
|
|
|
86
|
|
|
|
|
|
|
our (@ISA, @EXPORT, @EXPORT_OK); |
87
|
|
|
|
|
|
|
push @ISA, 'Exporter'; |
88
|
|
|
|
|
|
|
@EXPORT = qw( |
89
|
|
|
|
|
|
|
@program_commands |
90
|
|
|
|
|
|
|
%command_prefixes |
91
|
|
|
|
|
|
|
%composite_commands |
92
|
|
|
|
|
|
|
@program_params |
93
|
|
|
|
|
|
|
@program_switches |
94
|
|
|
|
|
|
|
%param_translation |
95
|
|
|
|
|
|
|
%command_files |
96
|
|
|
|
|
|
|
); |
97
|
|
|
|
|
|
|
|
98
|
|
|
|
|
|
|
@EXPORT_OK = qw(); |
99
|
|
|
|
|
|
|
|
100
|
|
|
|
|
|
|
|
101
|
|
|
|
|
|
|
|
102
|
|
|
|
|
|
|
our @program_commands = qw( |
103
|
|
|
|
|
|
|
run |
104
|
|
|
|
|
|
|
fasta2bfa |
105
|
|
|
|
|
|
|
fastq2bfq |
106
|
|
|
|
|
|
|
map |
107
|
|
|
|
|
|
|
mapmerge |
108
|
|
|
|
|
|
|
rmdup |
109
|
|
|
|
|
|
|
assemble |
110
|
|
|
|
|
|
|
indelpe |
111
|
|
|
|
|
|
|
indelsoa |
112
|
|
|
|
|
|
|
sol2sanger |
113
|
|
|
|
|
|
|
bfq2fastq |
114
|
|
|
|
|
|
|
mapass2maq |
115
|
|
|
|
|
|
|
mapview |
116
|
|
|
|
|
|
|
mapcheck |
117
|
|
|
|
|
|
|
pileup |
118
|
|
|
|
|
|
|
cns2fq |
119
|
|
|
|
|
|
|
cns2snp |
120
|
|
|
|
|
|
|
cns2view |
121
|
|
|
|
|
|
|
cns2ref |
122
|
|
|
|
|
|
|
cns2win |
123
|
|
|
|
|
|
|
fasta2csfa |
124
|
|
|
|
|
|
|
csmap2nt |
125
|
|
|
|
|
|
|
submap |
126
|
|
|
|
|
|
|
eland2maq |
127
|
|
|
|
|
|
|
export2maq |
128
|
|
|
|
|
|
|
); |
129
|
|
|
|
|
|
|
|
130
|
|
|
|
|
|
|
# composite commands: pseudo-commands that run a |
131
|
|
|
|
|
|
|
# sequence of commands |
132
|
|
|
|
|
|
|
# composite command prefix => list of prefixes of commands this |
133
|
|
|
|
|
|
|
# composite command runs |
134
|
|
|
|
|
|
|
# |
135
|
|
|
|
|
|
|
|
136
|
|
|
|
|
|
|
our %composite_commands = ( |
137
|
|
|
|
|
|
|
'run' => [qw( map asm c2q )] |
138
|
|
|
|
|
|
|
); |
139
|
|
|
|
|
|
|
|
140
|
|
|
|
|
|
|
# prefixes only for commands that take params/switches... |
141
|
|
|
|
|
|
|
our %command_prefixes = ( |
142
|
|
|
|
|
|
|
'fastq2bfq' => 'q2q', |
143
|
|
|
|
|
|
|
'map' => 'map', |
144
|
|
|
|
|
|
|
'assemble' => 'asm', |
145
|
|
|
|
|
|
|
'mapview' => 'mv', |
146
|
|
|
|
|
|
|
'mapcheck' => 'mck', |
147
|
|
|
|
|
|
|
'pileup' => 'pup', |
148
|
|
|
|
|
|
|
'cns2fq' => 'c2q', |
149
|
|
|
|
|
|
|
'cns2win' => 'c2w', |
150
|
|
|
|
|
|
|
'submap' => 'sub', |
151
|
|
|
|
|
|
|
'eland2maq' => 'l2m', |
152
|
|
|
|
|
|
|
'export2maq' => 'x2m', |
153
|
|
|
|
|
|
|
'run' => 'run' |
154
|
|
|
|
|
|
|
); |
155
|
|
|
|
|
|
|
|
156
|
|
|
|
|
|
|
our @program_params = qw( |
157
|
|
|
|
|
|
|
command |
158
|
|
|
|
|
|
|
q2q|n |
159
|
|
|
|
|
|
|
map|adaptor_file |
160
|
|
|
|
|
|
|
map|first_read_length |
161
|
|
|
|
|
|
|
map|max_hits |
162
|
|
|
|
|
|
|
map|max_mismatches |
163
|
|
|
|
|
|
|
map|max_outer_distance |
164
|
|
|
|
|
|
|
map|max_outer_distance_rf |
165
|
|
|
|
|
|
|
map|mismatch_dump |
166
|
|
|
|
|
|
|
map|mismatch_posn_dump |
167
|
|
|
|
|
|
|
map|mismatch_thr |
168
|
|
|
|
|
|
|
map|mutation_rate |
169
|
|
|
|
|
|
|
map|second_read_length |
170
|
|
|
|
|
|
|
map|unmapped_dump |
171
|
|
|
|
|
|
|
asm|error_dep_coeff |
172
|
|
|
|
|
|
|
asm|het_fraction |
173
|
|
|
|
|
|
|
asm|max_mismatches |
174
|
|
|
|
|
|
|
asm|max_quality_sum |
175
|
|
|
|
|
|
|
asm|min_map_quality |
176
|
|
|
|
|
|
|
asm|num_haplotypes |
177
|
|
|
|
|
|
|
mck|max_mismatches |
178
|
|
|
|
|
|
|
mck|min_map_quality |
179
|
|
|
|
|
|
|
pup|max_mismatches |
180
|
|
|
|
|
|
|
pup|max_quality_vals |
181
|
|
|
|
|
|
|
pup|min_map_quality |
182
|
|
|
|
|
|
|
pup|site_input_file |
183
|
|
|
|
|
|
|
c2q|min_map_quality |
184
|
|
|
|
|
|
|
c2q|min_read_depth |
185
|
|
|
|
|
|
|
c2q|min_nbr_quality |
186
|
|
|
|
|
|
|
c2q|max_read_depth |
187
|
|
|
|
|
|
|
c2w|window_size |
188
|
|
|
|
|
|
|
c2w|ref_seq |
189
|
|
|
|
|
|
|
c2w|start_posn |
190
|
|
|
|
|
|
|
c2w|end_posn |
191
|
|
|
|
|
|
|
c2w|min_cons_quality |
192
|
|
|
|
|
|
|
sub|max_mismatches |
193
|
|
|
|
|
|
|
sub|max_quality_sum |
194
|
|
|
|
|
|
|
sub|min_map_quality |
195
|
|
|
|
|
|
|
l2q|def_qual |
196
|
|
|
|
|
|
|
x2m|max_outer_distance |
197
|
|
|
|
|
|
|
x2m|first_read_length |
198
|
|
|
|
|
|
|
x2m|second_read_length |
199
|
|
|
|
|
|
|
); |
200
|
|
|
|
|
|
|
|
201
|
|
|
|
|
|
|
our @program_switches = qw( |
202
|
|
|
|
|
|
|
asm|single_end_quality |
203
|
|
|
|
|
|
|
asm|discard_wrong_pairs |
204
|
|
|
|
|
|
|
mv|omit_seq_qual |
205
|
|
|
|
|
|
|
mv|show_mismatch_posns |
206
|
|
|
|
|
|
|
mck|single_end_quality |
207
|
|
|
|
|
|
|
pup|single_end_quality |
208
|
|
|
|
|
|
|
pup|discard_wrong_pairs |
209
|
|
|
|
|
|
|
pup|verbose |
210
|
|
|
|
|
|
|
pup|show_base_posn |
211
|
|
|
|
|
|
|
sub|discard_wrong_pairs |
212
|
|
|
|
|
|
|
x2m|retain_filt_reads |
213
|
|
|
|
|
|
|
); |
214
|
|
|
|
|
|
|
|
215
|
|
|
|
|
|
|
our %param_translation = ( |
216
|
|
|
|
|
|
|
'q2q|n' => 'n', |
217
|
|
|
|
|
|
|
'map|max_mismatches' => 'n', |
218
|
|
|
|
|
|
|
'map|max_outer_distance' => 'a', |
219
|
|
|
|
|
|
|
'map|max_outer_distance_rf' => 'A', |
220
|
|
|
|
|
|
|
'map|first_read_length' => '1', |
221
|
|
|
|
|
|
|
'map|second_read_length' => '2', |
222
|
|
|
|
|
|
|
'map|mutation_rate' => 'm', |
223
|
|
|
|
|
|
|
'map|adaptor_file' => 'd', |
224
|
|
|
|
|
|
|
'map|unmapped_dump' => 'u', |
225
|
|
|
|
|
|
|
'map|mismatch_thr' => 'e', |
226
|
|
|
|
|
|
|
'map|mismatch_dump' => 'H', |
227
|
|
|
|
|
|
|
'map|max_hits' => 'C', |
228
|
|
|
|
|
|
|
'map|mismatch_posn_dump' => 'N', |
229
|
|
|
|
|
|
|
'asm|error_dep_coeff' => 't', |
230
|
|
|
|
|
|
|
'asm|het_fraction' => 'r', |
231
|
|
|
|
|
|
|
'asm|max_mismatches' => 'm', |
232
|
|
|
|
|
|
|
'asm|max_quality_sum' => 'Q', |
233
|
|
|
|
|
|
|
'asm|min_map_quality' => 'q', |
234
|
|
|
|
|
|
|
'asm|num_haplotypes' => 'N', |
235
|
|
|
|
|
|
|
'mck|max_mismatches' => 'm', |
236
|
|
|
|
|
|
|
'mck|min_map_quality' => 'q', |
237
|
|
|
|
|
|
|
'pup|max_mismatches' => 'm', |
238
|
|
|
|
|
|
|
'pup|max_quality_vals' => 'Q', |
239
|
|
|
|
|
|
|
'pup|min_map_quality' => 'q', |
240
|
|
|
|
|
|
|
'pup|site_input_file' => 'l', |
241
|
|
|
|
|
|
|
'c2q|min_map_quality' => 'Q', |
242
|
|
|
|
|
|
|
'c2q|min_read_depth' => 'd', |
243
|
|
|
|
|
|
|
'c2q|min_nbr_quality' => 'n', |
244
|
|
|
|
|
|
|
'c2q|max_read_depth' => 'D', |
245
|
|
|
|
|
|
|
'c2w|window_size' => 'w', |
246
|
|
|
|
|
|
|
'c2w|ref_seq' => 'c', |
247
|
|
|
|
|
|
|
'c2w|start_posn' => 'b', |
248
|
|
|
|
|
|
|
'c2w|end_posn' => 'e', |
249
|
|
|
|
|
|
|
'c2w|min_cons_quality' => 'q', |
250
|
|
|
|
|
|
|
'sub|max_mismatches' => 'm', |
251
|
|
|
|
|
|
|
'sub|max_quality_sum' => 'Q', |
252
|
|
|
|
|
|
|
'sub|min_map_quality' => 'q', |
253
|
|
|
|
|
|
|
'l2q|def_qual' => 'q', |
254
|
|
|
|
|
|
|
'x2m|max_outer_distance' => 'a', |
255
|
|
|
|
|
|
|
'x2m|first_read_length' => '1', |
256
|
|
|
|
|
|
|
'x2m|second_read_length' => '2', |
257
|
|
|
|
|
|
|
'asm|single_end_quality' => 's', |
258
|
|
|
|
|
|
|
'asm|discard_wrong_pairs' => 'p', |
259
|
|
|
|
|
|
|
'mv|omit_seq_qual' => 'b', |
260
|
|
|
|
|
|
|
'mv|show_mismatch_posns' => 'N', |
261
|
|
|
|
|
|
|
'mck|single_end_quality' => 's', |
262
|
|
|
|
|
|
|
'pup|single_end_quality' => 's', |
263
|
|
|
|
|
|
|
'pup|discard_wrong_pairs' => 'p', |
264
|
|
|
|
|
|
|
'pup|verbose' => 'v', |
265
|
|
|
|
|
|
|
'pup|show_base_posn' => 'P', |
266
|
|
|
|
|
|
|
'sub|discard_wrong_pairs' => 'p', |
267
|
|
|
|
|
|
|
'x2m|retain_filt_reads' => 'n' |
268
|
|
|
|
|
|
|
); |
269
|
|
|
|
|
|
|
|
270
|
|
|
|
|
|
|
# |
271
|
|
|
|
|
|
|
# the order in the arrayrefs is the order required |
272
|
|
|
|
|
|
|
# on the command line |
273
|
|
|
|
|
|
|
# |
274
|
|
|
|
|
|
|
# the strings in the arrayrefs (less special chars) |
275
|
|
|
|
|
|
|
# become the keys for named parameters to run_maq |
276
|
|
|
|
|
|
|
# |
277
|
|
|
|
|
|
|
# special chars: |
278
|
|
|
|
|
|
|
# |
279
|
|
|
|
|
|
|
# '#' implies optional |
280
|
|
|
|
|
|
|
# '*' implies variable number of this type |
281
|
|
|
|
|
|
|
# <|> implies stdin/stdout redirect |
282
|
|
|
|
|
|
|
# |
283
|
|
|
|
|
|
|
|
284
|
|
|
|
|
|
|
our %command_files = ( |
285
|
|
|
|
|
|
|
'run' => [qw( faq fas faq )], |
286
|
|
|
|
|
|
|
'fastq2bfq' => [qw( faq bfq )], |
287
|
|
|
|
|
|
|
'fasta2bfa' => [qw( fas bfa )], |
288
|
|
|
|
|
|
|
'map' => [qw( map bfa bfq1 #bfq2 2>#log )], |
289
|
|
|
|
|
|
|
'mapmerge' => [qw( out_map *in_map )], |
290
|
|
|
|
|
|
|
'rmdup' => [qw( out_map in_map )], |
291
|
|
|
|
|
|
|
'assemble' => [qw( cns bfa map 2>#log )], |
292
|
|
|
|
|
|
|
'indelpe' => [qw( bfa map >txt )], |
293
|
|
|
|
|
|
|
'indelsoa' => [qw( bfa map >txt )], |
294
|
|
|
|
|
|
|
'sol2sanger' => [qw( in_faq out_faq )], |
295
|
|
|
|
|
|
|
'bfq2fastq' => [qw( bfq faq )], |
296
|
|
|
|
|
|
|
'mapass2maq' => [qw( in_map out_map )], |
297
|
|
|
|
|
|
|
'mapview' => [qw( map >txt )], |
298
|
|
|
|
|
|
|
'mapcheck' => [qw( bfa map >txt )], |
299
|
|
|
|
|
|
|
'pileup' => [qw( bfa map >txt )], |
300
|
|
|
|
|
|
|
'cns2fq' => [qw( cns >faq )], |
301
|
|
|
|
|
|
|
'cns2snp' => [qw( cns >txt )], |
302
|
|
|
|
|
|
|
'cns2view' => [qw( cns >txt )], |
303
|
|
|
|
|
|
|
'cns2ref' => [qw( cns >fas )], |
304
|
|
|
|
|
|
|
'cns2win' => [qw( cns >txt )], |
305
|
|
|
|
|
|
|
'fasta2csfa' => [qw( in_fas >out_fas )], |
306
|
|
|
|
|
|
|
'csmap2nt' => [qw( out_map bfa in_map )], |
307
|
|
|
|
|
|
|
'submap' => [qw( out_map in_map )], |
308
|
|
|
|
|
|
|
'eland2maq' => [qw( map lis eld )], |
309
|
|
|
|
|
|
|
'export2maq' => [qw( map lis xpt )] |
310
|
|
|
|
|
|
|
); |
311
|
|
|
|
|
|
|
|
312
|
|
|
|
|
|
|
INIT { |
313
|
|
|
|
|
|
|
# add subcommand params and switches for |
314
|
|
|
|
|
|
|
# composite commands |
315
|
1
|
|
|
1
|
|
65
|
my @sub_params; |
316
|
|
|
|
|
|
|
my @sub_switches; |
317
|
1
|
|
|
|
|
4
|
foreach my $cmd (keys %composite_commands) { |
318
|
1
|
|
|
|
|
1
|
foreach my $subcmd ( @{$composite_commands{$cmd}} ) { |
|
1
|
|
|
|
|
3
|
|
319
|
3
|
|
|
|
|
81
|
my @sub_program_params = grep /^$subcmd\|/, @program_params; |
320
|
3
|
|
|
|
|
30
|
my @sub_program_switches = grep /^$subcmd\|/, @program_switches; |
321
|
3
|
|
|
|
|
5
|
for (@sub_program_params) { |
322
|
22
|
|
|
|
|
57
|
m/^$subcmd\|(.*)/; |
323
|
22
|
|
|
|
|
39
|
push @sub_params, "$cmd\|${subcmd}_".$1; |
324
|
|
|
|
|
|
|
} |
325
|
3
|
|
|
|
|
5
|
for (@sub_program_switches) { |
326
|
2
|
|
|
|
|
8
|
m/^$subcmd\|(.*)/; |
327
|
2
|
|
|
|
|
7
|
push @sub_switches, "$cmd\|${subcmd}_".$1; |
328
|
|
|
|
|
|
|
} |
329
|
|
|
|
|
|
|
} |
330
|
|
|
|
|
|
|
} |
331
|
1
|
|
|
|
|
4
|
push @program_params, @sub_params; |
332
|
1
|
|
|
|
|
3
|
push @program_switches, @sub_switches; |
333
|
|
|
|
|
|
|
# translations for subcmd params/switches not necessary |
334
|
|
|
|
|
|
|
} |
335
|
|
|
|
|
|
|
1; |