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# BioPerl module for Bio::Tools::Seg |
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# |
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# Copyright Balamurugan Kumarasamy |
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# Totally re-written, added docs and tests -- Torsten Seemann, Sep 2006 |
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# |
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# Copyright |
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# You may distribute this module under the same terms as perl itself |
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# |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::Tools::Seg - parse C output |
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=head1 SYNOPSIS |
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use Bio::Tools::Seg; |
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my $parser = Bio::Tools::Seg->(-file => 'seg.fasta'); |
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while ( my $f = $parser->next_result ) { |
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if ($f->score < 1.5) { |
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print $f->location->to_FTstring, " is low complexity\n"; |
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} |
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} |
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=head1 DESCRIPTION |
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C identifies low-complexity regions on a protein sequence. |
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It is usually part of the C and C packages. |
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The L module will only parse the "fasta" output |
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modes of C, i.e. C (low complexity regions only), |
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C (high complexity regions only), or C (both low |
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and high). |
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It creates a L for each FASTA-like entry |
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found in the input file. It is up to the user to appropriately filter |
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these using the feature's score. |
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=head1 FEEDBACK |
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=head2 Mailing Lists |
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User feedback is an integral part of the evolution of this and other |
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Bioperl modules. Send your comments and suggestions preferably to |
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the Bioperl mailing list. Your participation is much appreciated. |
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bioperl-l@bioperl.org - General discussion |
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http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
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=head2 Support |
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Please direct usage questions or support issues to the mailing list: |
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I |
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rather than to the module maintainer directly. Many experienced and |
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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=head2 Reporting Bugs |
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Report bugs to the Bioperl bug tracking system to help us keep track |
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of the bugs and their resolution. Bug reports can be submitted via the |
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web: |
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https://github.com/bioperl/bioperl-live/issues |
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=head1 AUTHOR - Torsten Seemann |
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Email - torsten.seemann AT infotech.monash.edu.au |
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=head1 CONTRIBUTOR - Bala |
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Email - savikalpa@fugu-sg.org |
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=head1 APPENDIX |
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The rest of the documentation details each of the object methods. |
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Internal methods are usually preceded with a _ |
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=cut |
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package Bio::Tools::Seg; |
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use strict; |
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use Bio::SeqFeature::Generic; |
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use base qw(Bio::Root::Root Bio::Root::IO); |
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=head2 new |
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Title : new |
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Usage : my $obj = Bio::Tools::Seg->new(); |
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Function: Builds a new Bio::Tools::Seg object |
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Returns : Bio::Tools::Seg |
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Args : -fh/-file => $val, # for initing input, see Bio::Root::IO |
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=cut |
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sub new { |
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my($class,@args) = @_; |
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my $self = $class->SUPER::new(@args); |
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$self->_initialize_io(@args); |
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return $self; |
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} |
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=head2 next_result |
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Title : next_result |
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Usage : my $feat = $seg->next_result |
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Function: Get the next result set from parser data |
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Returns : Bio::SeqFeature::Generic |
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Args : none |
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=cut |
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sub next_result { |
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my ($self) = @_; |
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# For example in this line |
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# test_prot(214-226) complexity=2.26 (12/2.20/2.50) |
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# $1 is test_prot |
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# $2 is 214 |
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# $3 is 226 |
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# $4 is 2.26 |
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while (my $line = $self->_readline) { |
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if ($line =~ /^\>\s*?(\S+)?\s*?\((\d+)\-(\d+)\)\s*complexity=(\S+)/) { |
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return Bio::SeqFeature::Generic->new( |
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-seq_id => $1, |
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-start => $2, |
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-end => $3, |
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-score => $4, |
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-source_tag => 'Seg', |
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-primary => 'low_complexity' |
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); |
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} |
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} |
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} |
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1; |