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# BioPerl module for Bio::Tools::HMMER::Domain |
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# Please direct questions and support issues to |
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# Cared for by Ewan Birney |
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# Copyright Ewan Birney |
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# |
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# You may distribute this module under the same terms as perl itself |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::Tools::HMMER::Domain - One particular domain hit from HMMER |
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=head1 SYNOPSIS |
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Read the Bio::Tools::HMMER::Results docs |
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=head1 DESCRIPTION |
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A particular domain score. We reuse the Homol SeqFeature system |
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here, so this inherits off Homol SeqFeature. As this code |
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originally came from a separate project, there are some backward |
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compatibility stuff provided to keep this working with old code. |
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Don't forget this inherits off Bio::SeqFeature, so all your usual |
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nice start/end/score stuff is ready for use. |
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=head1 CONTACT |
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Ewan Birney, birney@ebi.ac.uk |
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=head1 CONTRIBUTORS |
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Jason Stajich, jason@bioperl.org |
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=head1 APPENDIX |
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The rest of the documentation details each of the object |
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methods. Internal methods are usually preceded with a _ |
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=cut |
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#' |
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package Bio::Tools::HMMER::Domain; |
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use Bio::SeqFeature::Generic; |
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use strict; |
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use base qw(Bio::SeqFeature::FeaturePair); |
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sub new { |
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2434
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my($class,@args) = @_; |
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my $self = $class->SUPER::new(@args); |
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2434
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3695
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$self->{'alignlines'} = []; |
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2434
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my $hmmf1 = Bio::SeqFeature::Generic->new(@args); |
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my $hmmf2 = Bio::SeqFeature::Generic->new(@args); |
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2434
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$self->feature1($hmmf1); |
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$self->feature2($hmmf2); |
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return $self; |
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} |
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=head2 add_alignment_line |
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Title : add_alignment_line |
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Usage : $domain->add_alignment_line($line_from_hmmer_output); |
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Function: add an alignment line to this Domain object |
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Returns : Nothing |
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Args : scalar |
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Adds an alignment line, mainly for storing the HMMER alignments |
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as flat text which can be reguritated. You're right. This is *not |
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nice* and not the right way to do it. C'est la vie. |
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=cut |
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sub add_alignment_line { |
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my $self = shift; |
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my $line = shift; |
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push(@{$self->{'alignlines'}},$line); |
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} |
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=head2 each_alignment_line |
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Title : each_alignment_line |
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Usage : foreach $line ( $domain->each_alignment_line ) |
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Function: reguritates the alignment lines as they were fed in. |
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only useful realistically for printing. |
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Example : |
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Returns : |
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Args : None |
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=cut |
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sub each_alignment_line { |
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my $self = shift; |
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return @{$self->{'alignlines'}}; |
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} |
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=head2 get_nse |
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Title : get_nse |
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Usage : $domain->get_nse() |
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Function: Provides a seqname/start-end format, useful |
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for unique keys. nse stands for name-start-end |
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It is used a lot in Pfam |
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Example : |
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Returns : A string |
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Args : Optional separator 1 and separator 2 (default / and -) |
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=cut |
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sub get_nse { |
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1
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my $self = shift; |
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2755
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my $sep1 = shift; |
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3137
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my $sep2 = shift; |
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3038
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4380
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if( !defined $sep2 ) { |
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3119
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$sep2 = "-"; |
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} |
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if( !defined $sep1 ) { |
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$sep1 = "/"; |
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} |
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return sprintf("%s%s%d%s%d",$self->seq_id,$sep1,$self->start,$sep2,$self->end); |
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} |
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141
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# =head2 start_seq |
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# Title : start_seq |
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# Usage : Backward compatibility with old HMMER modules. |
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# should use $domain->start |
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# Function: |
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# Example : |
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# Returns : |
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# Args : |
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151
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# =cut |
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153
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sub start_seq { |
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my $self = shift; |
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my $start = shift; |
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$self->warn("Using old domain->start_seq. Should use domain->start"); |
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return $self->start($start); |
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} |
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# =head2 end_seq |
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# Title : end_seq |
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# Usage : Backward compatibility with old HMMER modules. |
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# should use $domain->end |
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# Function: |
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# Example : |
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# Returns : |
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# Args : |
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171
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# =cut |
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173
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sub end_seq { |
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my $self = shift; |
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my $end = shift; |
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$self->warn("Using old domain->end_seq. Should use domain->end"); |
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return $self->end($end); |
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} |
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# =head2 start_hmm |
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183
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# Title : start_hmm |
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# Usage : Backward compatibility with old HMMER modules, and |
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# for convience. Equivalent to $self->homol_SeqFeature->start |
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# Function: |
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# Example : |
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# Returns : |
189
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# Args : |
190
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191
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# =cut |
192
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193
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sub start_hmm { |
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my $self = shift; |
195
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my $start = shift; |
196
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$self->warn("Using old domain->start_hmm. Should use domain->hstart"); |
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return $self->hstart($start); |
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} |
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200
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# =head2 end_hmm |
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202
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# Title : end_hmm |
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# Usage : Backward compatibility with old HMMER modules, and |
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# for convience. Equivalent to $self->homol_SeqFeature->start |
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# Function: |
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# Example : |
207
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# Returns : |
208
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# Args : |
209
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210
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# =cut |
211
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212
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sub end_hmm { |
213
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0
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0
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0
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0
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my $self = shift; |
214
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0
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0
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my $end = shift; |
215
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216
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0
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0
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$self->warn("Using old domain->end_hmm. Should use domain->hend"); |
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0
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0
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return $self->hend($end); |
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} |
219
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220
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=head2 hmmacc |
221
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222
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Title : hmmacc |
223
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Usage : $domain->hmmacc($newacc) |
224
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Function: set get for HMM accession number. This is placed in the homol |
225
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feature of the HMM |
226
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Example : |
227
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Returns : |
228
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Args : |
229
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230
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231
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=cut |
232
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233
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sub hmmacc{ |
234
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1
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1
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1
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3
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my ($self,$acc) = @_; |
235
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1
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50
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2
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if( defined $acc ) { |
236
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1
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3
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$self->feature2->add_tag_value('accession',$acc); |
237
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} |
238
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1
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4
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my @vals = $self->feature2->each_tag_value('accession'); |
239
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1
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2
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return shift @vals; |
240
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} |
241
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242
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=head2 hmmname |
243
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244
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Title : hmmname |
245
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Usage : $domain->hmmname($newname) |
246
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Function: set get for HMM accession number. This is placed in the homol |
247
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feature of the HMM |
248
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Example : |
249
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Returns : |
250
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Args : |
251
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252
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=cut |
253
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254
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sub hmmname { |
255
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2437
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2437
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1
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3650
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return shift->hseq_id(@_); |
256
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} |
257
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258
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=head2 bits |
259
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260
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Title : bits |
261
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Usage : |
262
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Function: backward compatibility. Same as score |
263
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Example : |
264
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Returns : |
265
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Args : |
266
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267
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=cut |
268
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269
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sub bits{ |
270
|
3145
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3145
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1
|
5725
|
return shift->score(@_); |
271
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} |
272
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273
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|
=head2 evalue |
274
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275
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Title : evalue |
276
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Usage : |
277
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|
Function: $domain->evalue($value); |
278
|
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Example : |
279
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Returns : |
280
|
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Args : |
281
|
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282
|
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|
=cut |
283
|
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284
|
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|
|
sub evalue{ |
285
|
2435
|
|
|
2435
|
1
|
4820
|
return shift->_tag_value('evalue',@_); |
286
|
|
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|
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|
|
} |
287
|
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|
|
288
|
|
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|
|
=head2 seqbits |
289
|
|
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|
|
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|
|
290
|
|
|
|
|
|
|
Title : seqbits |
291
|
|
|
|
|
|
|
Usage : |
292
|
|
|
|
|
|
|
Function: $domain->seqbits($value); |
293
|
|
|
|
|
|
|
Example : |
294
|
|
|
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|
|
|
Returns : |
295
|
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|
|
Args : |
296
|
|
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|
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|
|
297
|
|
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|
|
|
|
=cut |
298
|
|
|
|
|
|
|
|
299
|
|
|
|
|
|
|
sub seqbits { |
300
|
2434
|
|
|
2434
|
1
|
3903
|
return shift->_tag_value('seqbits',@_); |
301
|
|
|
|
|
|
|
} |
302
|
|
|
|
|
|
|
|
303
|
|
|
|
|
|
|
=head2 seq_range |
304
|
|
|
|
|
|
|
|
305
|
|
|
|
|
|
|
Title : seq_range |
306
|
|
|
|
|
|
|
Usage : |
307
|
|
|
|
|
|
|
Function: Throws an exception to catch scripts which need to upgrade |
308
|
|
|
|
|
|
|
Example : |
309
|
|
|
|
|
|
|
Returns : |
310
|
|
|
|
|
|
|
Args : |
311
|
|
|
|
|
|
|
|
312
|
|
|
|
|
|
|
=cut |
313
|
|
|
|
|
|
|
|
314
|
|
|
|
|
|
|
sub seq_range{ |
315
|
0
|
|
|
0
|
1
|
|
my ($self,@args) = @_; |
316
|
|
|
|
|
|
|
|
317
|
0
|
|
|
|
|
|
$self->throw("You have accessed an old method. Please recode your script to the new bioperl HMMER module"); |
318
|
|
|
|
|
|
|
} |
319
|
|
|
|
|
|
|
|
320
|
|
|
|
|
|
|
=head2 hmm_range |
321
|
|
|
|
|
|
|
|
322
|
|
|
|
|
|
|
Title : hmm_range |
323
|
|
|
|
|
|
|
Usage : |
324
|
|
|
|
|
|
|
Function: Throws an exception to catch scripts which need to upgrade |
325
|
|
|
|
|
|
|
Example : |
326
|
|
|
|
|
|
|
Returns : |
327
|
|
|
|
|
|
|
Args : |
328
|
|
|
|
|
|
|
|
329
|
|
|
|
|
|
|
|
330
|
|
|
|
|
|
|
=cut |
331
|
|
|
|
|
|
|
|
332
|
|
|
|
|
|
|
sub hmm_range{ |
333
|
0
|
|
|
0
|
1
|
|
my ($self,@args) = @_; |
334
|
|
|
|
|
|
|
|
335
|
0
|
|
|
|
|
|
$self->throw("You have accessed an old method. Please recode your script to the new bioperl HMMER module"); |
336
|
|
|
|
|
|
|
} |
337
|
|
|
|
|
|
|
|
338
|
|
|
|
|
|
|
1; # says use was ok |
339
|
|
|
|
|
|
|
__END__ |