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# BioPerl module for Bio::SeqIO::FTHelper |
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# |
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# Please direct questions and support issues to |
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# |
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# Cared for by Ewan Birney |
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# |
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# Copyright Ewan Birney |
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# |
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# You may distribute this module under the same terms as perl itself |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::SeqIO::FTHelper - Helper class for EMBL/Genbank feature tables |
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=head1 SYNOPSIS |
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Used by Bio::SeqIO::EMBL,Bio::SeqIO::genbank, and Bio::SeqIO::swiss to |
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help process the Feature Table |
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=head1 DESCRIPTION |
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Represents one particular Feature with the following fields |
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key - the key of the feature |
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loc - the location string of the feature |
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- other fields |
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=head1 FEEDBACK |
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=head2 Mailing Lists |
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User feedback is an integral part of the evolution of this and other |
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Bioperl modules. Send your comments and suggestions preferably to one |
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of the Bioperl mailing lists. Your participation is much appreciated. |
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bioperl-l@bioperl.org - General discussion |
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http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
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=head2 Support |
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Please direct usage questions or support issues to the mailing list: |
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I |
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rather than to the module maintainer directly. Many experienced and |
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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=head2 Reporting Bugs |
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Report bugs to the Bioperl bug tracking system to help us keep track |
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the bugs and their resolution. Bug reports can be submitted via the web: |
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https://github.com/bioperl/bioperl-live/issues |
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=head1 AUTHOR - Ewan Birney |
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Email birney@ebi.ac.uk |
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=head1 CONTRIBUTORS |
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Jason Stajich jason@bioperl.org |
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=head1 APPENDIX |
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70
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The rest of the documentation details each of the object |
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methods. Internal methods are usually preceded with a _ |
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=cut |
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# Let the code begin... |
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79
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package Bio::SeqIO::FTHelper; |
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use strict; |
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745
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6054
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use Bio::SeqFeature::Generic; |
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836
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use Bio::Location::Simple; |
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use Bio::Location::Fuzzy; |
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use Bio::Location::Split; |
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use base qw(Bio::Root::Root); |
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15422
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sub new { |
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9457
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1
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my ($class, @args) = @_; |
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# no chained new because we make lots and lots of these. |
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my $self = {}; |
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9457
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bless $self,$class; |
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$self->{'_field'} = {}; |
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return $self; |
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} |
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=head2 _generic_seqfeature |
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Title : _generic_seqfeature |
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Usage : $fthelper->_generic_seqfeature($annseq, "GenBank") |
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Function: processes fthelper into a generic seqfeature |
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Returns : TRUE on success and otherwise FALSE |
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Args : The Bio::Factory::LocationFactoryI object to use for parsing |
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location strings. The ID (e.g., display_id) of the sequence on which |
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this feature is located, optionally a string indicating the source |
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(GenBank/EMBL/SwissProt) |
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=cut |
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sub _generic_seqfeature { |
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my ($fth, $locfac, $seqid, $source) = @_; |
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my ($sf); |
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# set a default if not specified |
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if(! defined($source)) { |
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$source = "EMBL/GenBank/SwissProt"; |
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} |
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# initialize feature object |
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$sf = Bio::SeqFeature::Generic->direct_new(); |
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# parse location; this may cause an exception, in which case we gently |
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# recover and ignore this feature |
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128
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my $loc; |
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eval { |
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$loc = $locfac->from_string($fth->loc); |
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}; |
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if(! $loc) { |
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0
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$fth->warn("exception while parsing location line [" . $fth->loc . |
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"] in reading $source, ignoring feature " . |
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$fth->key() . " (seqid=" . $seqid . "): " . $@); |
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0
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return; |
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} |
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# set additional location attributes |
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if($seqid && (! $loc->is_remote())) { |
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$loc->seq_id($seqid); # propagates if it is a split location |
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} |
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# set attributes of feature |
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$sf->location($loc); |
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$sf->primary_tag($fth->key); |
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$sf->source_tag($source); |
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$sf->seq_id($seqid); |
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foreach my $key ( keys %{$fth->field} ){ |
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15653
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41091
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foreach my $value ( @{$fth->field->{$key}} ) { |
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$sf->add_tag_value($key,$value); |
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} |
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} |
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8980
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return $sf; |
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} |
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159
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160
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=head2 from_SeqFeature |
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162
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Title : from_SeqFeature |
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Usage : @fthelperlist = Bio::SeqIO::FTHelper::from_SeqFeature($sf, |
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$context_annseq); |
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Function: constructor of fthelpers from SeqFeatures |
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: |
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: The additional annseq argument is to allow the building of FTHelper |
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: lines relevant to particular sequences (ie, when features are spread over |
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: enteries, knowing how to build this) |
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Returns : an array of FThelpers |
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Args : seq features |
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172
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173
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174
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=cut |
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176
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sub from_SeqFeature { |
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477
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477
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1
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my ($sf, $context_annseq) = @_; |
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477
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501
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my @ret; |
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180
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# |
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# If this object knows how to make FThelpers, then let it |
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182
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# - this allows us to store *really* weird objects that can write |
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183
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# themselves to the EMBL/GenBank... |
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184
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# |
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185
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186
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477
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50
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1885
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if ( $sf->can("to_FTHelper") ) { |
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0
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0
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return $sf->to_FTHelper($context_annseq); |
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} |
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189
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190
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477
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1081
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my $fth = Bio::SeqIO::FTHelper->new(); |
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191
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477
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1105
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my $key = $sf->primary_tag(); |
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192
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477
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1006
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my $locstr = $sf->location->to_FTstring; |
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193
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194
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# ES 25/06/01 Commented out this code, Jason to double check |
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195
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#The location FT string for all simple subseqfeatures is already |
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196
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#in the Split location FT string |
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197
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198
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# going into sub features |
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199
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#foreach my $sub ( $sf->sub_SeqFeature() ) { |
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200
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#my @subfth = &Bio::SeqIO::FTHelper::from_SeqFeature($sub); |
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201
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#push(@ret, @subfth); |
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202
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#} |
|
203
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204
|
477
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1101
|
$fth->loc($locstr); |
|
205
|
477
|
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|
857
|
$fth->key($key); |
|
206
|
477
|
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|
905
|
$fth->field->{'note'} = []; |
|
207
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208
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# the lines below take specific tags (e.g. /score=23 ) and re-enter them as |
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209
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|
|
# new tags like /note="score=25" - if the file is round-tripped this creates |
|
210
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|
# duplicate values |
|
211
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212
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|
|
#$sf->source_tag && do { push(@{$fth->field->{'note'}},"source=" . $sf->source_tag ); }; |
|
213
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214
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#($sf->can('score') && $sf->score) && do { push(@{$fth->field->{'note'}}, |
|
215
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|
|
# "score=" . $sf->score ); }; |
|
216
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217
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#($sf->can('frame') && $sf->frame) && do { push(@{$fth->field->{'note'}}, |
|
218
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|
|
# "frame=" . $sf->frame ); }; |
|
219
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220
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|
|
#$sf->strand && do { push(@{$fth->field->{'note'}},"strand=" . $sf->strand ); }; |
|
221
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222
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477
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|
996
|
foreach my $tag ( $sf->get_all_tags ) { |
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223
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|
# Tags which begin with underscores are considered |
|
224
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|
|
# private, and are therefore not printed |
|
225
|
953
|
50
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|
1880
|
next if $tag =~ /^_/; |
|
226
|
953
|
100
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|
1249
|
if ( !defined $fth->field->{$tag} ) { |
|
227
|
646
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|
946
|
$fth->field->{$tag} = []; |
|
228
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|
|
} |
|
229
|
953
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|
1671
|
foreach my $val ( $sf->get_tag_values($tag) ) { |
|
230
|
1233
|
|
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|
1292
|
push(@{$fth->field->{$tag}},$val); |
|
|
1233
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|
1484
|
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|
231
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|
|
} |
|
232
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|
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} |
|
233
|
477
|
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|
793
|
push(@ret, $fth); |
|
234
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|
235
|
477
|
50
|
|
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|
782
|
unless (@ret) { |
|
236
|
0
|
|
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|
|
0
|
$context_annseq->throw("Problem in processing seqfeature $sf - no fthelpers. Error!"); |
|
237
|
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|
|
} |
|
238
|
477
|
|
|
|
|
612
|
foreach my $ft (@ret) { |
|
239
|
477
|
50
|
|
|
|
1368
|
if ( !$ft->isa('Bio::SeqIO::FTHelper') ) { |
|
240
|
0
|
|
|
|
|
0
|
$sf->throw("Problem in processing seqfeature $sf - made a $fth!"); |
|
241
|
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|
|
} |
|
242
|
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|
|
} |
|
243
|
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|
244
|
477
|
|
|
|
|
1086
|
return @ret; |
|
245
|
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|
|
} |
|
246
|
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|
247
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|
248
|
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|
|
=head2 key |
|
249
|
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|
250
|
|
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|
|
Title : key |
|
251
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|
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|
|
Usage : $obj->key($newval) |
|
252
|
|
|
|
|
|
|
Function: |
|
253
|
|
|
|
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|
|
Example : |
|
254
|
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|
|
|
Returns : value of key |
|
255
|
|
|
|
|
|
|
Args : newvalue (optional) |
|
256
|
|
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|
|
257
|
|
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|
258
|
|
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|
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|
|
=cut |
|
259
|
|
|
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|
|
260
|
|
|
|
|
|
|
sub key { |
|
261
|
19420
|
|
|
19420
|
1
|
26472
|
my ($obj, $value) = @_; |
|
262
|
19420
|
100
|
|
|
|
28484
|
if ( defined $value ) { |
|
263
|
9457
|
|
|
|
|
14805
|
$obj->{'key'} = $value; |
|
264
|
|
|
|
|
|
|
} |
|
265
|
19420
|
|
|
|
|
38313
|
return $obj->{'key'}; |
|
266
|
|
|
|
|
|
|
|
|
267
|
|
|
|
|
|
|
} |
|
268
|
|
|
|
|
|
|
|
|
269
|
|
|
|
|
|
|
=head2 loc |
|
270
|
|
|
|
|
|
|
|
|
271
|
|
|
|
|
|
|
Title : loc |
|
272
|
|
|
|
|
|
|
Usage : $obj->loc($newval) |
|
273
|
|
|
|
|
|
|
Function: |
|
274
|
|
|
|
|
|
|
Example : |
|
275
|
|
|
|
|
|
|
Returns : value of loc |
|
276
|
|
|
|
|
|
|
Args : newvalue (optional) |
|
277
|
|
|
|
|
|
|
|
|
278
|
|
|
|
|
|
|
|
|
279
|
|
|
|
|
|
|
=cut |
|
280
|
|
|
|
|
|
|
|
|
281
|
|
|
|
|
|
|
sub loc { |
|
282
|
18917
|
|
|
18917
|
1
|
27247
|
my ($obj, $value) = @_; |
|
283
|
18917
|
100
|
|
|
|
27006
|
if ( defined $value ) { |
|
284
|
9457
|
|
|
|
|
14160
|
$obj->{'loc'} = $value; |
|
285
|
|
|
|
|
|
|
} |
|
286
|
18917
|
|
|
|
|
38624
|
return $obj->{'loc'}; |
|
287
|
|
|
|
|
|
|
} |
|
288
|
|
|
|
|
|
|
|
|
289
|
|
|
|
|
|
|
|
|
290
|
|
|
|
|
|
|
=head2 field |
|
291
|
|
|
|
|
|
|
|
|
292
|
|
|
|
|
|
|
Title : field |
|
293
|
|
|
|
|
|
|
Usage : |
|
294
|
|
|
|
|
|
|
Function: |
|
295
|
|
|
|
|
|
|
Example : |
|
296
|
|
|
|
|
|
|
Returns : |
|
297
|
|
|
|
|
|
|
Args : |
|
298
|
|
|
|
|
|
|
|
|
299
|
|
|
|
|
|
|
|
|
300
|
|
|
|
|
|
|
=cut |
|
301
|
|
|
|
|
|
|
|
|
302
|
|
|
|
|
|
|
sub field { |
|
303
|
169692
|
|
|
169692
|
1
|
183344
|
my ($self) = @_; |
|
304
|
|
|
|
|
|
|
|
|
305
|
169692
|
|
|
|
|
440949
|
return $self->{'_field'}; |
|
306
|
|
|
|
|
|
|
} |
|
307
|
|
|
|
|
|
|
|
|
308
|
|
|
|
|
|
|
=head2 add_field |
|
309
|
|
|
|
|
|
|
|
|
310
|
|
|
|
|
|
|
Title : add_field |
|
311
|
|
|
|
|
|
|
Usage : |
|
312
|
|
|
|
|
|
|
Function: |
|
313
|
|
|
|
|
|
|
Example : |
|
314
|
|
|
|
|
|
|
Returns : |
|
315
|
|
|
|
|
|
|
Args : |
|
316
|
|
|
|
|
|
|
|
|
317
|
|
|
|
|
|
|
|
|
318
|
|
|
|
|
|
|
=cut |
|
319
|
|
|
|
|
|
|
|
|
320
|
|
|
|
|
|
|
sub add_field { |
|
321
|
0
|
|
|
0
|
1
|
|
my ($self, $key, $val) = @_; |
|
322
|
|
|
|
|
|
|
|
|
323
|
0
|
0
|
|
|
|
|
if ( !exists $self->field->{$key} ) { |
|
324
|
0
|
|
|
|
|
|
$self->field->{$key} = []; |
|
325
|
|
|
|
|
|
|
} |
|
326
|
0
|
|
|
|
|
|
push( @{$self->field->{$key}} , $val); |
|
|
0
|
|
|
|
|
|
|
|
327
|
|
|
|
|
|
|
|
|
328
|
|
|
|
|
|
|
} |
|
329
|
|
|
|
|
|
|
|
|
330
|
|
|
|
|
|
|
1; |