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# BioPerl module for Bio::Seq::MetaI |
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# |
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# Please direct questions and support issues to |
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# |
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# Cared for by Heikki Lehvaslaiho |
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# |
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# Copyright Heikki Lehvaslaiho |
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# |
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# You may distribute this module under the same terms as perl itself |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::Seq::MetaI - Interface for sequence objects with residue-based |
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meta information |
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=head1 SYNOPSIS |
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# get a Bio::Seq::MetaI compliant object somehow |
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# to test this is a meta seq object |
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$obj->isa("Bio::Seq::MetaI") |
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|| $obj->throw("$obj not a Bio::Seq::MetaI"); |
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# accessors |
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$string = $obj->meta; |
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$string = $obj->meta_text; |
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$substring = $obj->submeta(12,50); |
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$unique_key = $obj->accession_number(); |
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=head1 DESCRIPTION |
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This class defines an abstract interface for basic residue-based meta |
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information. Examples of this kind of meta data are secondary |
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structures (RNA and protein), protein hydrophobicity assignments, or |
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other alternative alphabets for polypeptides, sequence quality data |
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and nucleotide alignments with translations. |
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The length of the meta data sequence is not dependent on the amount of |
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the meta information. The meta information always covers all the |
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residues, but a blank value is used to denote unavailable |
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information. If necessary the implementation quietly truncates or |
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extends meta information with blank values. Definition of blank is |
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implementation dependent. Gaps in MSAs should not have meta |
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information. |
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At this point a residue in a sequence object can have only one meta |
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value. If you need more, use multiple copies of the sequence object. |
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Meta data storage can be implemented in various ways, e.g: string, |
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array of scalars, array of hashes, array of objects. |
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If the implementation so chooses, there can be more then one meta |
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values associated to each residue. See L and |
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L. Note that use of arbitrary names is very prone to |
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typos leading to creation of additional copies of meta data sets. |
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Bio::Seq::Meta provides basic, pure perl implementation of sequences |
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with meta information. See L. Application specific |
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implementations will override and add to these methods. |
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=head2 Method naming |
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Character based meta data is read and set by method meta() and its |
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variants. These are the suffixes and prefixes used in the variants: |
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[named_] [sub] meta [_text] |
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=over 3 |
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=item _text |
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Suffix B<_text> guaranties that output is a string. Note that it does |
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not limit the input. |
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=item sub |
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Prefix B, like in subseq(), means that the method applies to sub |
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region of the sequence range and takes start and end as arguments. |
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Unlike subseq(), these methods are able to set values. If the range |
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is not defined, it defaults to the complete sequence. |
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=item named_ |
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Prefix B in method names allows the used to attach multiple |
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meta strings to one sequence by explicitly naming them. The name is |
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always the first argument to the method. The "unnamed" methods use the |
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class wide default name for the meta data and are thus special cases |
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"named" methods. |
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Note that internally names are keys in a hash and any misspelling of a |
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name will silently store the data under a wrong name. The used names |
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(keys) can be retrieved using method meta_names(). See L. |
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=back |
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=head1 SEE ALSO |
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L, |
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L, |
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L, |
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L, |
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L |
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=head1 FEEDBACK |
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=head2 Mailing Lists |
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User feedback is an integral part of the evolution of this and other |
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Bioperl modules. Send your comments and suggestions preferably to one |
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of the Bioperl mailing lists. Your participation is much appreciated. |
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bioperl-l@bioperl.org - General discussion |
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http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
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=head2 Support |
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Please direct usage questions or support issues to the mailing list: |
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I |
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rather than to the module maintainer directly. Many experienced and |
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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=head2 Reporting Bugs |
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Report bugs to the Bioperl bug tracking system to help us keep track |
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the bugs and their resolution. Bug reports can be submitted via the |
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web: |
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https://github.com/bioperl/bioperl-live/issues |
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=head1 AUTHOR - Heikki Lehvaslaiho |
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Email heikki-at-bioperl-dot-org |
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=head1 CONTRIBUTORS |
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Chad Matsalla, bioinformatics@dieselwurks.com; |
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Aaron Mackey, amackey@virginia.edu; |
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Peter Schattner schattner@alum.mit.edu; |
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Richard Adams, Richard.Adams@ed.ac.uk |
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=head1 APPENDIX |
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The rest of the documentation details each of the object methods. |
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Internal methods are usually preceded with a _ |
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=cut |
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# Let the code begin... |
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package Bio::Seq::MetaI; |
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use strict; |
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use base qw(Bio::Root::RootI); |
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=head2 meta |
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Title : meta |
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Usage : $meta_values = $obj->meta($values_string); |
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Function: |
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Get and set method for the unnamed meta data starting from |
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residue position one. Since it is dependent on the length |
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of the sequence, it needs to be manipulated after the |
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sequence. |
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The implementation may choose to accept argument values in |
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a string or in an array (reference) or in a hash |
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(reference). |
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The return value may be a string or an array reference, |
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depending on the implementation. If in doubt, use meta_text() |
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which is a variant guarantied to return a string. See |
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L. |
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The length of the returned value always matches the length |
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of the sequence. |
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Returns : A reference to an array or a string |
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Args : new value, optional |
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=cut |
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sub meta { shift->throw_not_implemented } |
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=head2 meta_text |
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Title : meta_text() |
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Usage : $meta_values = $obj->meta_text($values_arrayref); |
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Function: Variant of meta() guarantied to return a textual |
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representation of the meta data. For details, see L. |
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Returns : a string |
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Args : new value, optional |
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=cut |
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sub meta_text { shift->throw_not_implemented } |
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=head2 named_meta |
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Title : named_meta() |
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Usage : $meta_values = $obj->named_meta($name, $values_arrayref); |
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Function: A more general version of meta(). Each meta data set needs |
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to be named. See also L. |
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Returns : a string |
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Args : scalar, name of the meta data set |
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new value, optional |
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=cut |
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sub named_meta { shift->throw_not_implemented } |
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=head2 named_meta_text |
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Title : named_meta_text() |
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Usage : $meta_values = $obj->named_meta_text($name, $values_arrayref); |
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Function: Variant of named_meta() guarantied to return a textual |
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representation of the named meta data. |
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For details, see L. |
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Returns : a string |
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Args : scalar, name of the meta data set |
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new value, optional |
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=cut |
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sub named_meta_text { shift->throw_not_implemented } |
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=head2 submeta |
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Title : submeta |
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Usage : $subset_of_meta_values = $obj->submeta(10, 20, $value_string); |
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$subset_of_meta_values = $obj->submeta(10, undef, $value_string); |
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Function: |
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Get and set method for meta data for subsequences. |
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Numbering starts from 1 and the number is inclusive, ie 1-2 |
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are the first two residue of the sequence. Start cannot be |
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larger than end but can be equal. |
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If the second argument is missing the returned values |
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should extend to the end of the sequence. |
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If implementation tries to set values beyond the current |
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sequence, they should be ignored. |
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The return value may be a string or an array reference, |
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depending on the implementation. If in doubt, use |
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submeta_text() which is a variant guarantied to return a |
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string. See L. |
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Returns : A reference to an array or a string |
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Args : integer, start position, optional |
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integer, end position, optional |
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new value, optional |
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=cut |
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sub submeta { shift->throw_not_implemented } |
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=head2 submeta_text |
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Title : submeta_text |
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Usage : $meta_values = $obj->submeta_text(20, $value_string); |
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Function: Variant of submeta() guarantied to return a textual |
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representation of meta data. For details, see L. |
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Returns : a string |
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Args : integer, start position, optional |
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integer, end position, optional |
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new value, optional |
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=cut |
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sub submeta_text { shift->throw_not_implemented } |
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=head2 named_submeta |
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Title : named_submeta |
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Usage : $subset_of_meta_values = $obj->named_submeta($name, 10, 20, $value_string); |
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$subset_of_meta_values = $obj->named_submeta($name, 10); |
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Function: Variant of submeta() guarantied to return a textual |
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representation of meta data. For details, see L. |
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Returns : A reference to an array or a string |
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Args : scalar, name of the meta data set |
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integer, start position |
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integer, end position, optional when a third argument present |
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new value, optional |
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=cut |
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sub named_submeta { shift->throw_not_implemented } |
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=head2 named_submeta_text |
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306
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Title : named_submeta_text |
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Usage : $meta_values = $obj->named_submeta_text($name, 20, $value_string); |
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Function: Variant of submeta() guarantied to return a textual |
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representation of meta data. For details, see L. |
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Returns : a string |
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Args : scalar, name of the meta data |
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Args : integer, start position, optional |
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integer, end position, optional |
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new value, optional |
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=cut |
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318
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sub named_submeta_text { shift->throw_not_implemented } |
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320
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=head2 meta_names |
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322
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Title : meta_names |
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Usage : @meta_names = $obj->meta_names() |
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Function: Retrieves an array of meta data set names. The default (unnamed) |
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set name is guarantied to be the first name. |
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Returns : an array of names |
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Args : none |
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329
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=cut |
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331
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sub meta_names { shift->throw_not_implemented } |
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333
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334
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=head2 force_flush |
335
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336
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Title : force_flush() |
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Usage : $force_flush = $obj->force_flush(1); |
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Function: Automatically pad with empty values or truncate meta values to |
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sequence length |
340
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Returns : boolean 1 or 0 |
341
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Args : optional boolean value |
342
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343
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=cut |
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345
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sub force_flush { shift->throw_not_implemented } |
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347
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348
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=head2 is_flush |
349
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350
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Title : is_flush |
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Usage : $is_flush = $obj->is_flush() |
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or $is_flush = $obj->is_flush($my_meta_name) |
353
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Function: Boolean to tell if all meta values are in |
354
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flush with the sequence length. |
355
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Returns true if force_flush() is set |
356
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Set verbosity to a positive value to see failed meta sets |
357
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Returns : boolean 1 or 0 |
358
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Args : optional name of the meta set |
359
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360
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=cut |
361
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362
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0
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sub is_flush { shift->throw_not_implemented } |
363
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364
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365
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=head2 meta_length |
366
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367
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Title : meta_length() |
368
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Usage : $meeta_len = $obj->meta_length(); |
369
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Function: return the number of elements in the meta set |
370
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Returns : integer |
371
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Args : - |
372
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373
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=cut |
374
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375
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0
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0
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1
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sub meta_length { shift->throw_not_implemented } |
376
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377
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=head2 named_meta_length |
378
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379
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Title : named_meta_length() |
380
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Usage : $meeta_len = $obj->named_meta_length($name); |
381
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Function: return the number of elements in the named meta set |
382
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Returns : integer |
383
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Args : - |
384
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385
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=cut |
386
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387
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0
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0
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1
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sub named_meta_length { shift->throw_not_implemented } |
388
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389
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390
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=head1 Bio::PrimarySeqI methods |
391
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392
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Implementing classes will need to rewrite these Bio::PrimaryI methods. |
393
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394
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=cut |
395
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396
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=head2 revcom |
397
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398
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Title : revcom |
399
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Usage : $newseq = $seq->revcom(); |
400
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Function: Produces a new Bio::Seq::MetaI implementing object where |
401
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the order of residues and their meta information is reversed. |
402
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Returns : A new (fresh) Bio::Seq::MetaI object |
403
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Args : none |
404
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405
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=cut |
406
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407
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0
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0
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1
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sub revcom { shift->throw_not_implemented } |
408
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409
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=head2 trunc |
410
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411
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Title : trunc |
412
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Usage : $subseq = $myseq->trunc(10,100); |
413
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Function: Provides a truncation of a sequence |
414
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Returns : a fresh Bio::Seq::MetaI implementing object |
415
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Args : Two integers denoting first and last residue of the sub-sequence. |
416
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417
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=cut |
418
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419
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0
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0
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1
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sub trunc { shift->throw_not_implemented } |
420
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421
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422
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1; |