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#----------------------------------------------------------------- |
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# |
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# BioPerl module Bio::Search::Result::ResultI |
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# |
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# Please direct questions and support issues to |
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# |
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# Cared for by Steve Chervitz |
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# |
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# Originally created by Aaron Mackey |
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# |
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# You may distribute this module under the same terms as perl itself |
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#----------------------------------------------------------------- |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::Search::Result::ResultI - Abstract interface to Search Result objects |
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=head1 SYNOPSIS |
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# Bio::Search::Result::ResultI objects cannot be instantiated since this |
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# module defines a pure interface. |
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# Given an object that implements the Bio::Search::Result::ResultI interface, |
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# you can do the following things with it: |
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use Bio::SearchIO; |
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my $io = Bio::SearchIO->new(-format => 'blast', |
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-file => 't/data/HUMBETGLOA.tblastx'); |
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my $result = $io->next_result; |
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while( $hit = $result->next_hit()) { # enter code here for hit processing |
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} |
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my $id = $result->query_name(); |
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my $desc = $result->query_description(); |
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my $dbname = $result->database_name(); |
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my $size = $result->database_letters(); |
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my $num_entries = $result->database_entries(); |
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my $gap_ext = $result->get_parameter('gapext'); |
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my @params = $result->available_parameters; |
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my $kappa = $result->get_statistic('kappa'); |
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my @statnames = $result->available_statistics; |
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=head1 DESCRIPTION |
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Bio::Search::Result::ResultI objects are data structures containing |
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the results from the execution of a search algorithm. As such, it may |
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contain various algorithm specific information as well as details of |
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the execution, but will contain a few fundamental elements, including |
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the ability to return Bio::Search::Hit::HitI objects. |
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=head1 FEEDBACK |
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=head2 Mailing Lists |
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User feedback is an integral part of the evolution of this |
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and other Bioperl modules. Send your comments and suggestions preferably |
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to one of the Bioperl mailing lists. |
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Your participation is much appreciated. |
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bioperl-l@bioperl.org - General discussion |
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http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
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=head2 Support |
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Please direct usage questions or support issues to the mailing list: |
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I |
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rather than to the module maintainer directly. Many experienced and |
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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=head2 Reporting Bugs |
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Report bugs to the Bioperl bug tracking system to help us keep track |
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the bugs and their resolution. Bug reports can be submitted via the |
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web: |
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https://github.com/bioperl/bioperl-live/issues |
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=head1 AUTHOR |
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Aaron Mackey Eamackey@virginia.eduE (original author) |
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Steve Chervitz Esac@bioperl.orgE |
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See L for where to send bug reports and comments. |
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=head1 COPYRIGHT |
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103
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Copyright (c) 1999-2001 Aaron Mackey, Steve Chervitz. All Rights Reserved. |
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=head1 DISCLAIMER |
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This software is provided "as is" without warranty of any kind. |
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=head1 APPENDIX |
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The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ |
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=cut |
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#' |
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# Let the code begin... |
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package Bio::Search::Result::ResultI; |
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use strict; |
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use base qw(Bio::AnalysisResultI); |
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=head2 next_hit |
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129
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Title : next_hit |
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Usage : while( $hit = $result->next_hit()) { ... } |
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Function: Returns the next available Hit object, representing potential |
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matches between the query and various entities from the database. |
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Returns : a Bio::Search::Hit::HitI object or undef if there are no more. |
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Args : none |
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=cut |
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sub next_hit { |
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$self->throw_not_implemented; |
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} |
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=head2 sort_hits |
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Title : sort_hits |
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Usage : $result->sort_hits(\&sort_function) |
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Function : Sorts the available hit objects by a user-supplied function. Defaults to sort |
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by descending score. |
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Returns : n/a |
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Args : A coderef for the sort function. See the documentation on the Perl sort() |
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function for guidelines on writing sort functions. |
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Note : To access the special variables $a and $b used by the Perl sort() function |
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the user function must access Bio::Search::Result::ResultI namespace. |
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For example, use : |
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$result->sort_hits( sub{$Bio::Search::Result::ResultI::a->length <=> |
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$Bio::Search::Result::ResultI::b->length}); |
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NOT $result->sort_hits($a->length <=>$b->length); |
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=cut |
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sub sort_hits { |
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my ($self, $coderef) = @_; |
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my @sorted_hits; |
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if ($coderef) { |
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$self->throw('sort_hits requires a sort function passed as a subroutine reference') |
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unless (ref($coderef) eq 'CODE'); |
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} |
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else { |
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$coderef = \&_default_sort_hits; |
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# throw a warning? |
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} |
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my @hits = $self->hits(); |
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eval {@sorted_hits = sort $coderef @hits }; |
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if ($@) { |
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$self->throw("Unable to sort hits: $@"); |
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} |
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else { |
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$self->{'_hits'} = \@sorted_hits; |
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$self->{'_no_iterations'} = 1; # to bypass iteration checking in hits() method |
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1; |
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} |
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} |
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189
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=head2 _default sort_hits |
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191
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Title : _default_sort_hits |
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Usage : Do not call directly. |
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Function: Sort hits in descending order by score |
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Args : None |
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Returns: 1 on success |
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Note : Used by $result->sort_hits() |
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198
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=cut |
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200
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sub _default_sort_hits { |
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$Bio::Search::Result::ResultI::b->score <=> |
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$Bio::Search::Result::ResultI::a->score; |
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204
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} |
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206
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=head2 query_name |
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208
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Title : query_name |
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Usage : $id = $result->query_name(); |
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Function: Get the string identifier of the query used by the |
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algorithm that performed the search. |
212
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Returns : a string. |
213
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Args : none |
214
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215
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=cut |
216
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217
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sub query_name { |
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my ($self,@args) = @_; |
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$self->throw_not_implemented; |
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} |
221
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222
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=head2 query_accession |
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224
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Title : query_accession |
225
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Usage : $id = $result->query_accession(); |
226
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Function: Get the accession (if available) for the query sequence |
227
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Returns : a string |
228
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Args : none |
229
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230
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=cut |
231
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232
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sub query_accession { |
233
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0
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1
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my ($self,@args) = @_; |
234
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0
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$self->throw_not_implemented; |
235
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} |
236
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237
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238
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=head2 query_length |
239
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240
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|
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Title : query_length |
241
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|
Usage : $id = $result->query_length(); |
242
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|
Function: Get the length of the query sequence |
243
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used in the search. |
244
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|
Returns : a number |
245
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|
Args : none |
246
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247
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=cut |
248
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249
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sub query_length { |
250
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0
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0
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1
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|
my ($self,@args) = @_; |
251
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0
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|
$self->throw_not_implemented; |
252
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} |
253
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254
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=head2 query_description |
255
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256
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Title : query_description |
257
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Usage : $id = $result->query_description(); |
258
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Function: Get the description of the query sequence |
259
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used in the search. |
260
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Returns : a string |
261
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Args : none |
262
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263
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=cut |
264
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265
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sub query_description { |
266
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0
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0
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1
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|
my ($self,@args) = @_; |
267
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0
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|
$self->throw_not_implemented; |
268
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|
} |
269
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270
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271
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=head2 database_name |
272
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273
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Title : database_name |
274
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|
|
Usage : $name = $result->database_name() |
275
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|
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|
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|
|
Function: Used to obtain the name of the database that the query was searched |
276
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|
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|
|
against by the algorithm. |
277
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|
Returns : a scalar string |
278
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|
Args : none |
279
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280
|
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|
=cut |
281
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282
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|
sub database_name { |
283
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0
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|
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0
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1
|
|
my ($self,@args) = @_; |
284
|
|
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|
|
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|
|
285
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0
|
|
|
|
|
|
$self->throw_not_implemented; |
286
|
|
|
|
|
|
|
} |
287
|
|
|
|
|
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|
|
288
|
|
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|
|
=head2 database_letters |
289
|
|
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|
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290
|
|
|
|
|
|
|
Title : database_letters |
291
|
|
|
|
|
|
|
Usage : $size = $result->database_letters() |
292
|
|
|
|
|
|
|
Function: Used to obtain the size of database that was searched against. |
293
|
|
|
|
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|
|
Returns : a scalar integer (units specific to algorithm, but probably the |
294
|
|
|
|
|
|
|
total number of residues in the database, if available) or undef if |
295
|
|
|
|
|
|
|
the information was not available to the Processor object. |
296
|
|
|
|
|
|
|
Args : none |
297
|
|
|
|
|
|
|
|
298
|
|
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|
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|
|
|
299
|
|
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|
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|
=cut |
300
|
|
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|
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|
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301
|
|
|
|
|
|
|
sub database_letters { |
302
|
0
|
|
|
0
|
1
|
|
my ($self,@args) = @_; |
303
|
0
|
|
|
|
|
|
$self->throw_not_implemented(); |
304
|
|
|
|
|
|
|
} |
305
|
|
|
|
|
|
|
|
306
|
|
|
|
|
|
|
=head2 database_entries |
307
|
|
|
|
|
|
|
|
308
|
|
|
|
|
|
|
Title : database_entries |
309
|
|
|
|
|
|
|
Usage : $num_entries = $result->database_entries() |
310
|
|
|
|
|
|
|
Function: Used to obtain the number of entries contained in the database. |
311
|
|
|
|
|
|
|
Returns : a scalar integer representing the number of entities in the database |
312
|
|
|
|
|
|
|
or undef if the information was not available. |
313
|
|
|
|
|
|
|
Args : none |
314
|
|
|
|
|
|
|
|
315
|
|
|
|
|
|
|
|
316
|
|
|
|
|
|
|
=cut |
317
|
|
|
|
|
|
|
|
318
|
|
|
|
|
|
|
sub database_entries { |
319
|
0
|
|
|
0
|
1
|
|
my ($self,@args) = @_; |
320
|
|
|
|
|
|
|
|
321
|
0
|
|
|
|
|
|
$self->throw_not_implemented(); |
322
|
|
|
|
|
|
|
} |
323
|
|
|
|
|
|
|
|
324
|
|
|
|
|
|
|
=head2 get_parameter |
325
|
|
|
|
|
|
|
|
326
|
|
|
|
|
|
|
Title : get_parameter |
327
|
|
|
|
|
|
|
Usage : my $gap_ext = $result->get_parameter('gapext') |
328
|
|
|
|
|
|
|
Function: Returns the value for a specific parameter used |
329
|
|
|
|
|
|
|
when running this result |
330
|
|
|
|
|
|
|
Returns : string |
331
|
|
|
|
|
|
|
Args : name of parameter (string) |
332
|
|
|
|
|
|
|
|
333
|
|
|
|
|
|
|
=cut |
334
|
|
|
|
|
|
|
|
335
|
|
|
|
|
|
|
sub get_parameter{ |
336
|
0
|
|
|
0
|
1
|
|
my ($self,@args) = @_; |
337
|
0
|
|
|
|
|
|
$self->throw_not_implemented(); |
338
|
|
|
|
|
|
|
} |
339
|
|
|
|
|
|
|
|
340
|
|
|
|
|
|
|
=head2 available_parameters |
341
|
|
|
|
|
|
|
|
342
|
|
|
|
|
|
|
Title : available_parameters |
343
|
|
|
|
|
|
|
Usage : my @params = $result->available_parameters |
344
|
|
|
|
|
|
|
Function: Returns the names of the available parameters |
345
|
|
|
|
|
|
|
Returns : Return list of available parameters used for this result |
346
|
|
|
|
|
|
|
Args : none |
347
|
|
|
|
|
|
|
|
348
|
|
|
|
|
|
|
=cut |
349
|
|
|
|
|
|
|
|
350
|
|
|
|
|
|
|
sub available_parameters{ |
351
|
0
|
|
|
0
|
1
|
|
my ($self) = @_; |
352
|
0
|
|
|
|
|
|
$self->throw_not_implemented(); |
353
|
|
|
|
|
|
|
} |
354
|
|
|
|
|
|
|
|
355
|
|
|
|
|
|
|
=head2 get_statistic |
356
|
|
|
|
|
|
|
|
357
|
|
|
|
|
|
|
Title : get_statistic |
358
|
|
|
|
|
|
|
Usage : my $gap_ext = $result->get_statistic('kappa') |
359
|
|
|
|
|
|
|
Function: Returns the value for a specific statistic available |
360
|
|
|
|
|
|
|
from this result |
361
|
|
|
|
|
|
|
Returns : string |
362
|
|
|
|
|
|
|
Args : name of statistic (string) |
363
|
|
|
|
|
|
|
|
364
|
|
|
|
|
|
|
=cut |
365
|
|
|
|
|
|
|
|
366
|
|
|
|
|
|
|
sub get_statistic{ |
367
|
0
|
|
|
0
|
1
|
|
my ($self,@args) = @_; |
368
|
0
|
|
|
|
|
|
$self->throw_not_implemented(); |
369
|
|
|
|
|
|
|
} |
370
|
|
|
|
|
|
|
|
371
|
|
|
|
|
|
|
=head2 available_statistics |
372
|
|
|
|
|
|
|
|
373
|
|
|
|
|
|
|
Title : available_statistics |
374
|
|
|
|
|
|
|
Usage : my @statnames = $result->available_statistics |
375
|
|
|
|
|
|
|
Function: Returns the names of the available statistics |
376
|
|
|
|
|
|
|
Returns : Return list of available statistics used for this result |
377
|
|
|
|
|
|
|
Args : none |
378
|
|
|
|
|
|
|
|
379
|
|
|
|
|
|
|
=cut |
380
|
|
|
|
|
|
|
|
381
|
|
|
|
|
|
|
sub available_statistics{ |
382
|
0
|
|
|
0
|
1
|
|
my ($self) = @_; |
383
|
0
|
|
|
|
|
|
$self->throw_not_implemented(); |
384
|
|
|
|
|
|
|
} |
385
|
|
|
|
|
|
|
|
386
|
|
|
|
|
|
|
=head2 algorithm |
387
|
|
|
|
|
|
|
|
388
|
|
|
|
|
|
|
Title : algorithm |
389
|
|
|
|
|
|
|
Usage : my $r_type = $result->algorithm |
390
|
|
|
|
|
|
|
Function: Obtain the name of the algorithm used to obtain the Result |
391
|
|
|
|
|
|
|
Returns : string (e.g., BLASTP) |
392
|
|
|
|
|
|
|
Args : [optional] scalar string to set value |
393
|
|
|
|
|
|
|
|
394
|
|
|
|
|
|
|
=cut |
395
|
|
|
|
|
|
|
|
396
|
|
|
|
|
|
|
sub algorithm{ |
397
|
0
|
|
|
0
|
1
|
|
my ($self) = @_; |
398
|
0
|
|
|
|
|
|
$self->throw_not_implemented(); |
399
|
|
|
|
|
|
|
} |
400
|
|
|
|
|
|
|
|
401
|
|
|
|
|
|
|
=head2 algorithm_version |
402
|
|
|
|
|
|
|
|
403
|
|
|
|
|
|
|
Title : algorithm_version |
404
|
|
|
|
|
|
|
Usage : my $r_version = $result->algorithm_version |
405
|
|
|
|
|
|
|
Function: Obtain the version of the algorithm used to obtain the Result |
406
|
|
|
|
|
|
|
Returns : string (e.g., 2.1.2) |
407
|
|
|
|
|
|
|
Args : [optional] scalar string to set algorithm version value |
408
|
|
|
|
|
|
|
|
409
|
|
|
|
|
|
|
=cut |
410
|
|
|
|
|
|
|
|
411
|
|
|
|
|
|
|
sub algorithm_version{ |
412
|
0
|
|
|
0
|
1
|
|
my ($self) = @_; |
413
|
0
|
|
|
|
|
|
$self->throw_not_implemented(); |
414
|
|
|
|
|
|
|
} |
415
|
|
|
|
|
|
|
|
416
|
|
|
|
|
|
|
|
417
|
|
|
|
|
|
|
=head2 algorithm_reference |
418
|
|
|
|
|
|
|
|
419
|
|
|
|
|
|
|
Title : algorithm_reference |
420
|
|
|
|
|
|
|
Usage : $obj->algorithm_reference($newval) |
421
|
|
|
|
|
|
|
Function: |
422
|
|
|
|
|
|
|
Returns : value of the literature reference for the algorithm |
423
|
|
|
|
|
|
|
Args : newvalue (optional) |
424
|
|
|
|
|
|
|
Comments: The default implementation in ResultI returns an empty string |
425
|
|
|
|
|
|
|
rather than throwing a NotImplemented exception, since |
426
|
|
|
|
|
|
|
the ref may not always be available and is not critical. |
427
|
|
|
|
|
|
|
|
428
|
|
|
|
|
|
|
=cut |
429
|
|
|
|
|
|
|
|
430
|
|
|
|
|
|
|
sub algorithm_reference{ |
431
|
0
|
|
|
0
|
1
|
|
my ($self) = @_; |
432
|
0
|
|
|
|
|
|
return ''; |
433
|
|
|
|
|
|
|
} |
434
|
|
|
|
|
|
|
|
435
|
|
|
|
|
|
|
=head2 rid |
436
|
|
|
|
|
|
|
|
437
|
|
|
|
|
|
|
Title : rid |
438
|
|
|
|
|
|
|
Usage : $obj->rid($newval) |
439
|
|
|
|
|
|
|
Function: |
440
|
|
|
|
|
|
|
Returns : value of the BLAST Request ID (eg. RID: ZABJ4EA7014) |
441
|
|
|
|
|
|
|
Args : newvalue (optional) |
442
|
|
|
|
|
|
|
Comments: The default implementation in ResultI returns an empty string |
443
|
|
|
|
|
|
|
rather than throwing a NotImplemented exception, since |
444
|
|
|
|
|
|
|
the RID may not always be available and is not critical. |
445
|
|
|
|
|
|
|
See: (1) https://www.ncbi.nlm.nih.gov/Class/MLACourse/Modules/BLAST/rid.html |
446
|
|
|
|
|
|
|
(2) https://www.ncbi.nlm.nih.gov/staff/tao/URLAPI/new/node63.html |
447
|
|
|
|
|
|
|
=cut |
448
|
|
|
|
|
|
|
|
449
|
|
|
|
|
|
|
sub rid{ |
450
|
0
|
|
|
0
|
1
|
|
my ($self) = @_; |
451
|
0
|
|
|
|
|
|
return ''; |
452
|
|
|
|
|
|
|
} |
453
|
|
|
|
|
|
|
|
454
|
|
|
|
|
|
|
=head2 num_hits |
455
|
|
|
|
|
|
|
|
456
|
|
|
|
|
|
|
Title : num_hits |
457
|
|
|
|
|
|
|
Usage : my $hitcount= $result->num_hits |
458
|
|
|
|
|
|
|
Function: returns the number of hits for this query result |
459
|
|
|
|
|
|
|
Returns : integer |
460
|
|
|
|
|
|
|
Args : none |
461
|
|
|
|
|
|
|
|
462
|
|
|
|
|
|
|
|
463
|
|
|
|
|
|
|
=cut |
464
|
|
|
|
|
|
|
|
465
|
|
|
|
|
|
|
sub num_hits{ |
466
|
0
|
|
|
0
|
1
|
|
my ($self,@args) = @_; |
467
|
0
|
|
|
|
|
|
$self->throw_not_implemented(); |
468
|
|
|
|
|
|
|
} |
469
|
|
|
|
|
|
|
|
470
|
|
|
|
|
|
|
=head2 hits |
471
|
|
|
|
|
|
|
|
472
|
|
|
|
|
|
|
Title : hits |
473
|
|
|
|
|
|
|
Usage : my @hits = $result->hits |
474
|
|
|
|
|
|
|
Function: Returns the HitI objects contained within this Result |
475
|
|
|
|
|
|
|
Returns : Array of Bio::Search::Hit::HitI objects |
476
|
|
|
|
|
|
|
Args : none |
477
|
|
|
|
|
|
|
|
478
|
|
|
|
|
|
|
See Also: L |
479
|
|
|
|
|
|
|
|
480
|
|
|
|
|
|
|
=cut |
481
|
|
|
|
|
|
|
|
482
|
|
|
|
|
|
|
sub hits{ |
483
|
0
|
|
|
0
|
1
|
|
my ($self,@args) = @_; |
484
|
0
|
|
|
|
|
|
$self->throw_not_implemented(); |
485
|
|
|
|
|
|
|
} |
486
|
|
|
|
|
|
|
|
487
|
|
|
|
|
|
|
|
488
|
|
|
|
|
|
|
=head2 no_hits_found |
489
|
|
|
|
|
|
|
|
490
|
|
|
|
|
|
|
Usage : $nohits = $blast->no_hits_found(); |
491
|
|
|
|
|
|
|
Purpose : Get boolean indicator indicating whether or not any hits |
492
|
|
|
|
|
|
|
were present in the report. |
493
|
|
|
|
|
|
|
|
494
|
|
|
|
|
|
|
This is NOT the same as determining the number of hits via |
495
|
|
|
|
|
|
|
the hits() method, which will return zero hits if there were no |
496
|
|
|
|
|
|
|
hits in the report or if all hits were filtered out during the parse. |
497
|
|
|
|
|
|
|
|
498
|
|
|
|
|
|
|
Thus, this method can be used to distinguish these possibilities |
499
|
|
|
|
|
|
|
for hitless reports generated when filtering. |
500
|
|
|
|
|
|
|
|
501
|
|
|
|
|
|
|
Returns : Boolean |
502
|
|
|
|
|
|
|
Argument : none |
503
|
|
|
|
|
|
|
|
504
|
|
|
|
|
|
|
=cut |
505
|
|
|
|
|
|
|
|
506
|
|
|
|
|
|
|
#----------- |
507
|
0
|
|
|
0
|
1
|
|
sub no_hits_found { shift->throw_not_implemented } |
508
|
|
|
|
|
|
|
|
509
|
|
|
|
|
|
|
|
510
|
|
|
|
|
|
|
|
511
|
|
|
|
|
|
|
=head2 set_no_hits_found |
512
|
|
|
|
|
|
|
|
513
|
|
|
|
|
|
|
Usage : $blast->set_no_hits_found(); |
514
|
|
|
|
|
|
|
Purpose : Set boolean indicator indicating whether or not any hits |
515
|
|
|
|
|
|
|
were present in the report. |
516
|
|
|
|
|
|
|
Returns : n/a |
517
|
|
|
|
|
|
|
Argument : none |
518
|
|
|
|
|
|
|
|
519
|
|
|
|
|
|
|
=cut |
520
|
|
|
|
|
|
|
|
521
|
0
|
|
|
0
|
1
|
|
sub set_no_hits_found { shift->throw_not_implemented } |
522
|
|
|
|
|
|
|
|
523
|
|
|
|
|
|
|
1; |
524
|
|
|
|
|
|
|
|
525
|
|
|
|
|
|
|
|