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# BioPerl module for Bio::Search::Hit::GenericHit |
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# |
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# Please direct questions and support issues to |
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# |
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# Cared for by Jason Stajich |
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# |
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# Copyright Jason Stajich |
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# |
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# You may distribute this module under the same terms as perl itself |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::Search::Hit::BlastHit - Blast-specific subclass of Bio::Search::Hit::GenericHit |
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=head1 SYNOPSIS |
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use Bio::Search::Hit::BlastHit; |
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my $hit = Bio::Search::Hit::BlastHit->new(-algorithm => 'blastp'); |
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# See Bio::Search::Hit::GenericHit for information about working with Hits. |
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# TODO: Describe how to configure a SearchIO stream so that it generates |
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# GenericHit objects. |
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=head1 DESCRIPTION |
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This object is a subclass of Bio::Search::Hit::GenericHit |
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and provides some operations that facilitate working with BLAST |
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and PSI-BLAST Hits. |
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For general information about working with Hits, see |
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Bio::Search::Hit::GenericHit. |
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=head1 FEEDBACK |
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=head2 Mailing Lists |
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User feedback is an integral part of the evolution of this and other |
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Bioperl modules. Send your comments and suggestions preferably to |
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the Bioperl mailing list. Your participation is much appreciated. |
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bioperl-l@bioperl.org - General discussion |
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http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
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=head2 Support |
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Please direct usage questions or support issues to the mailing list: |
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I |
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rather than to the module maintainer directly. Many experienced and |
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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=head2 Reporting Bugs |
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Report bugs to the Bioperl bug tracking system to help us keep track |
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of the bugs and their resolution. Bug reports can be submitted via the |
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web: |
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https://github.com/bioperl/bioperl-live/issues |
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=head1 AUTHOR - Jason Stajich and Steve Chervitz |
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Email jason@bioperl.org |
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Email sac@bioperl.org |
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=head1 APPENDIX |
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The rest of the documentation details each of the object methods. |
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Internal methods are usually preceded with a _ |
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=cut |
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# Let the code begin... |
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package Bio::Search::Hit::BlastHit; |
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use strict; |
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use Bio::Search::SearchUtils; |
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use base qw(Bio::Search::Hit::GenericHit); |
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=head2 new |
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Title : new |
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Usage : my $obj = Bio::Search::Hit::GenericHit->new(); |
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Function: Builds a new Bio::Search::Hit::GenericHit object |
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Returns : Bio::Search::Hit::GenericHit |
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Args : See Bio::Search::Hit::GenericHit() for other args. |
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Here are the BLAST-specific args that can be used when |
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creating BlastHit objects: |
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-iteration => integer for the PSI-Blast iteration number |
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-found_again => boolean, true if hit appears in a |
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"previously found" section of a PSI-Blast report. |
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=cut |
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sub new { |
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my($class,@args) = @_; |
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my $self = $class->SUPER::new(@args); |
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my ($iter,$found) = $self->_rearrange([qw(ITERATION |
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FOUND_AGAIN |
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)], @args); |
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defined $iter && $self->iteration($iter); |
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defined $found && $self->found_again($found); |
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return $self; |
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} |
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=head2 iteration |
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Usage : $hit->iteration( $iteration_num ); |
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Purpose : Gets the iteration number in which the Hit was found. |
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Example : $iteration_num = $sbjct->iteration(); |
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Returns : Integer greater than or equal to 1 |
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Non-PSI-BLAST reports will report iteration as 1, but this number |
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is only meaningful for PSI-BLAST reports. |
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Argument : iteration_num (optional, used when setting only) |
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Throws : none |
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See Also : L |
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=cut |
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sub iteration{ |
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if( defined $value) { |
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$self->{'_psiblast_iteration'} = $value; |
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} |
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return $self->{'_psiblast_iteration'}; |
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} |
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=head2 found_again |
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Title : found_again |
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Usage : $hit->found_again; |
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$hit->found_again(1); |
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Purpose : Gets a boolean indicator whether or not the hit has |
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been found in a previous iteration. |
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This is only applicable to PSI-BLAST reports. |
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This method indicates if the hit was reported in the |
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"Sequences used in model and found again" section of the |
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PSI-BLAST report or if it was reported in the |
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"Sequences not found previously or not previously below threshold" |
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section of the PSI-BLAST report. Only for hits in iteration > 1. |
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Example : if( $hit->found_again()) { ... }; |
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Returns : Boolean, true (1) if the hit has been found in a |
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previous PSI-BLAST iteration. |
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Returns false (0 or undef) for hits that have not occurred in a |
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previous PSI-BLAST iteration. |
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Argument : Boolean (1 or 0). Only used for setting. |
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Throws : none |
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See Also : L |
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=cut |
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sub found_again { |
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return $self->{'_found_again'} = shift if @_; |
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return $self->{'_found_again'}; |
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} |
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sub expect { shift->significance(@_) } |
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1; |