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# BioPerl module for Bio::Map::LinkageMap |
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# |
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# Please direct questions and support issues to |
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# |
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# Cared for by Sendu Bala |
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# |
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# Copyright Chad Matsalla |
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# |
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# You may distribute this module under the same terms as perl itself |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::Map::LinkageMap - A representation of a genetic linkage map. |
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=head1 SYNOPSIS |
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use Bio::Map::LinkageMap; |
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# create a new map |
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my $map = Bio::Map::LinkageMap->new(-name => 'Chads Superterriffic Map', |
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-type => 'Linkage', |
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-units=> 'cM'); |
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# create the location of a marker for that map |
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my $position = Bio::Map::LinkagePosition->new( -positions => 1, |
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-distance => "22.3"); |
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# create a marker and place it at that position |
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my $marker = Bio::Map::Marker::Microsatellite->new( |
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-name => 'SuuuperMarker', |
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-position => $position); |
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# place that marker on that map |
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$map->add_element($marker); |
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# done! |
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=head1 DESCRIPTION |
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This object describes the basic functionality of a genetic linkage map in |
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Bioperl. Each 'position' can have one or more markers that map some number of |
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units from the markers at the previous position. |
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=head1 FEEDBACK |
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=head2 Mailing Lists |
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User feedback is an integral part of the evolution of this and other |
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Bioperl modules. Send your comments and suggestions preferably to |
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the Bioperl mailing list. Your participation is much appreciated. |
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bioperl-l@bioperl.org - General discussion |
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http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
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=head2 Support |
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Please direct usage questions or support issues to the mailing list: |
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I |
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rather than to the module maintainer directly. Many experienced and |
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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=head2 Reporting Bugs |
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Report bugs to the Bioperl bug tracking system to help us keep track |
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of the bugs and their resolution. Bug reports can be submitted via the |
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web: |
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https://github.com/bioperl/bioperl-live/issues |
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=head1 AUTHOR - Chad Matsalla |
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Email bioinformatics1@dieselwurks.com |
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=head1 CONTRIBUTORS |
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Lincoln Stein lstein@cshl.org |
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Heikki Lehvaslaiho heikki-at-bioperl-dot-org |
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Jason Stajich jason@bioperl.org |
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Sendu Bala bix@sendu.me.uk |
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=head1 APPENDIX |
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The rest of the documentation details each of the object methods. |
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Internal methods are usually preceded with a _ |
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=cut |
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# Let the code begin... |
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package Bio::Map::LinkageMap; |
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use strict; |
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use base qw(Bio::Map::SimpleMap); |
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=head2 new |
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Title : new |
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Usage : my $linkage_map = Bio::Map::LinkageMap->new(); |
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Function: Builds a new Bio::Map::LinkageMap object |
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Returns : Bio::Map::LinkageMap |
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Args : -name => the name of the map (string) [optional] |
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-type => the type of this map (string, defaults to Linkage) [optional] |
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-species => species for this map (Bio::Species) [optional] |
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-units => the map units (string, defaults to cM) [optional] |
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-elements=> elements to initialize with |
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(arrayref of Bio::Map::MappableI objects) [optional] |
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-uid => Unique ID of this map |
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=cut |
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=head2 length |
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Title : length |
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Usage : my $length = $map->length(); |
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Function: Retrieves the length of the map. In the case of a LinkageMap, the |
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length is the sum of all marker distances. |
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Returns : An integer representing the length of this LinkageMap. Will return |
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0 if length is not calculateable |
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Args : None. |
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=cut |
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sub length { |
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my ($self) = @_; |
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$self->throw("Not yet implemented correctly"); |
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my $total_distance; |
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foreach my $element (@{$self->get_elements}) { |
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#*** there is no such method ->each_position_value! |
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$total_distance += ($element->position->each_position_value($self))[0]; |
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} |
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return $total_distance; |
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} |
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=head2 add_element($marker) |
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Title : add_element($marker) |
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Usage : $map->add_element($marker) |
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Function: Add a Bio::Map::MappableI object to the Map |
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Returns : none |
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Args : Bio::Map::MappableI object |
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Notes : It is strongly recommended that you use a |
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Bio::Map::LinkagePosition as the position in any |
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Bio::Map::Mappable that you create to place on this |
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map. Using some other Bio::Map::Position might work but might |
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be unpredictable. |
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N.B. I've added Bio::Map::OrderedPosition which should achieve |
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similar things from LinkagePosition and will work for |
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RH markers too. |
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=cut |
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#*** what is this? what calls it? note that it seems to be private |
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sub _add_element_will_be_deleted { |
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my ($self,$marker) = @_; |
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my $o_position = $marker->position(); |
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$self->debug( "marker position is ". $marker->position()); |
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# print("add_element: \$o_position is $o_position\n"); |
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# print("add_element: \$marker is $marker\n"); |
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my $position; |
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unless ( $o_position->isa('Bio::Map::LinkagePosition') || |
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$o_position->isa('Bio::Map::OrderedPosition') |
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) { |
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$self->warn("You really should use a Linkage Position for this object. This insures that there is only one position. Trying anyway..."); |
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my @p = ( $o_position->each_position_value($self)); |
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$position = shift @p; |
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if( ! defined $position ) { |
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$self->throw("This marker ($marker) does not have a position in this map ($self)"); |
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} |
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} else { |
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$position = $o_position->order; |
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} |
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if ($self->{'_elements'}[$position]) { |
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$self->warn("Replacing the marker in position $position because in a linkage map the position is a key."); |
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} |
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$self->{'_elements'}[$position] = $marker; |
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} |
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1; |