line |
stmt |
bran |
cond |
sub |
pod |
time |
code |
1
|
|
|
|
|
|
|
# |
2
|
|
|
|
|
|
|
# bioperl module for Bio::LiveSeq::DNA |
3
|
|
|
|
|
|
|
# |
4
|
|
|
|
|
|
|
# Please direct questions and support issues to |
5
|
|
|
|
|
|
|
# |
6
|
|
|
|
|
|
|
# Cared for by Joseph Insana |
7
|
|
|
|
|
|
|
# |
8
|
|
|
|
|
|
|
# Copyright Joseph Insana |
9
|
|
|
|
|
|
|
# |
10
|
|
|
|
|
|
|
# You may distribute this module under the same terms as perl itself |
11
|
|
|
|
|
|
|
# |
12
|
|
|
|
|
|
|
# POD documentation - main docs before the code |
13
|
|
|
|
|
|
|
|
14
|
|
|
|
|
|
|
=head1 NAME |
15
|
|
|
|
|
|
|
|
16
|
|
|
|
|
|
|
Bio::LiveSeq::DNA - DNA object for LiveSeq |
17
|
|
|
|
|
|
|
|
18
|
|
|
|
|
|
|
=head1 SYNOPSIS |
19
|
|
|
|
|
|
|
|
20
|
|
|
|
|
|
|
# documentation needed |
21
|
|
|
|
|
|
|
|
22
|
|
|
|
|
|
|
=head1 DESCRIPTION |
23
|
|
|
|
|
|
|
|
24
|
|
|
|
|
|
|
This holds the DNA sequence (or the RNA in the case of cDNA entries) |
25
|
|
|
|
|
|
|
and is accessed by exons, genes, transcripts... objects |
26
|
|
|
|
|
|
|
|
27
|
|
|
|
|
|
|
=head1 AUTHOR - Joseph A.L. Insana |
28
|
|
|
|
|
|
|
|
29
|
|
|
|
|
|
|
Email: Insana@ebi.ac.uk, jinsana@gmx.net |
30
|
|
|
|
|
|
|
|
31
|
|
|
|
|
|
|
=head1 APPENDIX |
32
|
|
|
|
|
|
|
|
33
|
|
|
|
|
|
|
The rest of the documentation details each of the object |
34
|
|
|
|
|
|
|
methods. Internal methods are usually preceded with a _ |
35
|
|
|
|
|
|
|
|
36
|
|
|
|
|
|
|
=cut |
37
|
|
|
|
|
|
|
|
38
|
|
|
|
|
|
|
# Let the code begin... |
39
|
|
|
|
|
|
|
|
40
|
|
|
|
|
|
|
package Bio::LiveSeq::DNA; |
41
|
|
|
|
|
|
|
|
42
|
2
|
|
|
2
|
|
10
|
use strict; |
|
2
|
|
|
|
|
3
|
|
|
2
|
|
|
|
|
55
|
|
43
|
2
|
|
|
2
|
|
11
|
use base qw(Bio::LiveSeq::SeqI); |
|
2
|
|
|
|
|
4
|
|
|
2
|
|
|
|
|
621
|
|
44
|
|
|
|
|
|
|
|
45
|
|
|
|
|
|
|
=head2 new |
46
|
|
|
|
|
|
|
|
47
|
|
|
|
|
|
|
Title : new |
48
|
|
|
|
|
|
|
Usage : $dna = Bio::LiveSeq::DNA->new(-seq => "atcgaccaatggacctca", |
49
|
|
|
|
|
|
|
-offset => 3 ); |
50
|
|
|
|
|
|
|
|
51
|
|
|
|
|
|
|
Function: generates a new Bio::LiveSeq::DNA |
52
|
|
|
|
|
|
|
Returns : reference to a new object of class DNA |
53
|
|
|
|
|
|
|
Errorcode -1 |
54
|
|
|
|
|
|
|
Args : a string |
55
|
|
|
|
|
|
|
AND an optional offset to create nucleotide labels (default is 1, i.e. |
56
|
|
|
|
|
|
|
starting the count of labels from "1") -> do not bother using it -> |
57
|
|
|
|
|
|
|
it could be used by alternative loaders !EMBL format |
58
|
|
|
|
|
|
|
NOTE : strand of DNA is set to 1 by default |
59
|
|
|
|
|
|
|
|
60
|
|
|
|
|
|
|
=cut |
61
|
|
|
|
|
|
|
|
62
|
|
|
|
|
|
|
sub new { |
63
|
6
|
|
|
6
|
1
|
23
|
my ($thing, %args) = @_; |
64
|
6
|
|
33
|
|
|
25
|
my $class = ref($thing) || $thing; |
65
|
6
|
|
|
|
|
9
|
my (%empty,$obj); |
66
|
|
|
|
|
|
|
|
67
|
6
|
50
|
|
|
|
18
|
if ($args{-seq}) { |
68
|
6
|
|
|
|
|
47
|
$obj = $thing->string2chain($args{-seq},$args{-offset}); # inherited from ChainI |
69
|
6
|
|
|
|
|
35
|
$obj = bless $obj, $class; |
70
|
|
|
|
|
|
|
} else { |
71
|
0
|
|
|
|
|
0
|
$obj=\%empty; |
72
|
0
|
|
|
|
|
0
|
$obj = bless $obj, $class; |
73
|
0
|
|
|
|
|
0
|
$obj->throw("$class not initialized properly"); |
74
|
|
|
|
|
|
|
} |
75
|
|
|
|
|
|
|
|
76
|
6
|
|
|
|
|
46
|
$obj->{'alphabet'}='dna'; # set alphabet default |
77
|
6
|
|
|
|
|
16
|
$obj->{'strand'}=1; # set strand default = 1 |
78
|
6
|
|
|
|
|
15
|
$obj->{'seq'}=$obj; # set seq field to itself |
79
|
|
|
|
|
|
|
|
80
|
6
|
|
|
|
|
35
|
return $obj; |
81
|
|
|
|
|
|
|
} |
82
|
|
|
|
|
|
|
|
83
|
|
|
|
|
|
|
# START method |
84
|
|
|
|
|
|
|
# it has to be redefined here because default from SeqI accesses field "start" |
85
|
|
|
|
|
|
|
sub start { |
86
|
21
|
|
|
21
|
1
|
45
|
my $self = shift; |
87
|
21
|
|
|
|
|
79
|
return $self->{'begin'}; # the chain's start is called begin |
88
|
|
|
|
|
|
|
} |
89
|
|
|
|
|
|
|
|
90
|
|
|
|
|
|
|
# it is overridden to provide faster output |
91
|
|
|
|
|
|
|
sub length { |
92
|
0
|
|
|
0
|
1
|
0
|
my $self=shift; |
93
|
0
|
|
|
|
|
0
|
return $self->chain_length(); |
94
|
|
|
|
|
|
|
} |
95
|
|
|
|
|
|
|
|
96
|
|
|
|
|
|
|
# it is overridden to provide MUCH faster output |
97
|
|
|
|
|
|
|
sub valid { |
98
|
3594
|
|
|
3594
|
1
|
3838
|
my $self=shift(@_); |
99
|
3594
|
|
|
|
|
5350
|
return $self->label_exists(@_); |
100
|
|
|
|
|
|
|
} |
101
|
|
|
|
|
|
|
|
102
|
|
|
|
|
|
|
1; |