line |
stmt |
bran |
cond |
sub |
pod |
time |
code |
1
|
|
|
|
|
|
|
# $Id: Util.pm 15875 2009-07-21 19:20:00Z chmille4 $ |
2
|
|
|
|
|
|
|
# |
3
|
|
|
|
|
|
|
# BioPerl module for Bio::Nexml::Factory |
4
|
|
|
|
|
|
|
# |
5
|
|
|
|
|
|
|
# Please direct questions and support issues to |
6
|
|
|
|
|
|
|
# |
7
|
|
|
|
|
|
|
# Cared for by Chase Miller |
8
|
|
|
|
|
|
|
# |
9
|
|
|
|
|
|
|
# Copyright Chase Miller |
10
|
|
|
|
|
|
|
# |
11
|
|
|
|
|
|
|
# You may distribute this module under the same terms as perl itself |
12
|
|
|
|
|
|
|
|
13
|
|
|
|
|
|
|
# POD documentation - main docs before the code |
14
|
|
|
|
|
|
|
|
15
|
|
|
|
|
|
|
=head1 NAME |
16
|
|
|
|
|
|
|
|
17
|
|
|
|
|
|
|
Bio::Nexml::Factory - A factory module for creating BioPerl and Bio::Phylo objects from/to nexml documents |
18
|
|
|
|
|
|
|
|
19
|
|
|
|
|
|
|
=head1 SYNOPSIS |
20
|
|
|
|
|
|
|
|
21
|
|
|
|
|
|
|
Do not use this module directly. It shoulde be used through |
22
|
|
|
|
|
|
|
Bio::NexmlIO, Bio::SeqIO::nexml, Bio::AlignIO::nexml, or |
23
|
|
|
|
|
|
|
Bio::TreeIO::nexml |
24
|
|
|
|
|
|
|
|
25
|
|
|
|
|
|
|
|
26
|
|
|
|
|
|
|
=head1 DESCRIPTION |
27
|
|
|
|
|
|
|
|
28
|
|
|
|
|
|
|
This is a factory/utility module in the Nexml namespace. It contains |
29
|
|
|
|
|
|
|
methods that are needed by multiple modules. |
30
|
|
|
|
|
|
|
|
31
|
|
|
|
|
|
|
This module handles the creation of BioPerl objects from Bio::Phylo |
32
|
|
|
|
|
|
|
objects and vice versa, which is used to read and write nexml |
33
|
|
|
|
|
|
|
documents to and from BioPerl objects. |
34
|
|
|
|
|
|
|
|
35
|
|
|
|
|
|
|
=head1 FEEDBACK |
36
|
|
|
|
|
|
|
|
37
|
|
|
|
|
|
|
=head2 Mailing Lists |
38
|
|
|
|
|
|
|
|
39
|
|
|
|
|
|
|
User feedback is an integral part of the evolution of this and other |
40
|
|
|
|
|
|
|
Bioperl modules. Send your comments and suggestions preferably to |
41
|
|
|
|
|
|
|
the Bioperl mailing list. Your participation is much appreciated. |
42
|
|
|
|
|
|
|
|
43
|
|
|
|
|
|
|
bioperl-l@bioperl.org - General discussion |
44
|
|
|
|
|
|
|
http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
45
|
|
|
|
|
|
|
|
46
|
|
|
|
|
|
|
=head2 Support |
47
|
|
|
|
|
|
|
|
48
|
|
|
|
|
|
|
Please direct usage questions or support issues to the mailing list: |
49
|
|
|
|
|
|
|
|
50
|
|
|
|
|
|
|
I |
51
|
|
|
|
|
|
|
|
52
|
|
|
|
|
|
|
rather than to the module maintainer directly. Many experienced and |
53
|
|
|
|
|
|
|
reponsive experts will be able look at the problem and quickly |
54
|
|
|
|
|
|
|
address it. Please include a thorough description of the problem |
55
|
|
|
|
|
|
|
with code and data examples if at all possible. |
56
|
|
|
|
|
|
|
|
57
|
|
|
|
|
|
|
=head2 Reporting Bugs |
58
|
|
|
|
|
|
|
|
59
|
|
|
|
|
|
|
Report bugs to the Bioperl bug tracking system to help us keep track |
60
|
|
|
|
|
|
|
of the bugs and their resolution. Bug reports can be submitted via |
61
|
|
|
|
|
|
|
the web: |
62
|
|
|
|
|
|
|
|
63
|
|
|
|
|
|
|
https://github.com/bioperl/bioperl-live/issues |
64
|
|
|
|
|
|
|
|
65
|
|
|
|
|
|
|
=head1 AUTHOR - Chase Miller |
66
|
|
|
|
|
|
|
|
67
|
|
|
|
|
|
|
Email chmille4@gmail.com |
68
|
|
|
|
|
|
|
|
69
|
|
|
|
|
|
|
=head1 APPENDIX |
70
|
|
|
|
|
|
|
|
71
|
|
|
|
|
|
|
The rest of the documentation details each of the object methods. |
72
|
|
|
|
|
|
|
Internal methods are usually preceded with a _ |
73
|
|
|
|
|
|
|
|
74
|
|
|
|
|
|
|
=cut |
75
|
|
|
|
|
|
|
|
76
|
|
|
|
|
|
|
|
77
|
|
|
|
|
|
|
#Let the code begin |
78
|
|
|
|
|
|
|
|
79
|
|
|
|
|
|
|
package Bio::Nexml::Factory; |
80
|
|
|
|
|
|
|
|
81
|
3
|
|
|
3
|
|
9
|
use strict; |
|
3
|
|
|
|
|
3
|
|
|
3
|
|
|
|
|
77
|
|
82
|
|
|
|
|
|
|
|
83
|
|
|
|
|
|
|
BEGIN { |
84
|
3
|
|
|
3
|
|
384
|
use Bio::Root::Root; |
|
3
|
|
|
|
|
3
|
|
|
3
|
|
|
|
|
93
|
|
85
|
3
|
50
|
|
3
|
|
133
|
unless (eval "require Bio::Phylo; 1") { |
86
|
0
|
|
|
|
|
0
|
Bio::Root::Root->throw("Bio::Phylo package required; see http://www.nexml.org for download details"); |
87
|
|
|
|
|
|
|
} |
88
|
|
|
|
|
|
|
} |
89
|
|
|
|
|
|
|
|
90
|
3
|
|
|
3
|
|
1129
|
use Bio::Phylo::Factory; |
|
3
|
|
|
|
|
1779
|
|
|
3
|
|
|
|
|
15
|
|
91
|
3
|
|
|
3
|
|
1084
|
use Bio::Phylo::Matrices; |
|
3
|
|
|
|
|
47724
|
|
|
3
|
|
|
|
|
34
|
|
92
|
3
|
|
|
3
|
|
1272
|
use Bio::Phylo::Matrices::Matrix; |
|
3
|
|
|
|
|
43410
|
|
|
3
|
|
|
|
|
20
|
|
93
|
3
|
|
|
3
|
|
82
|
use Bio::Phylo::Matrices::Datum; |
|
3
|
|
|
|
|
3
|
|
|
3
|
|
|
|
|
13
|
|
94
|
3
|
|
|
3
|
|
1375
|
use Bio::Phylo::Forest::Tree; |
|
3
|
|
|
|
|
53319
|
|
|
3
|
|
|
|
|
25
|
|
95
|
3
|
|
|
3
|
|
78
|
use Bio::Phylo::Matrices; |
|
3
|
|
|
|
|
4
|
|
|
3
|
|
|
|
|
15
|
|
96
|
3
|
|
|
3
|
|
50
|
use Bio::Phylo::IO; |
|
3
|
|
|
|
|
3
|
|
|
3
|
|
|
|
|
85
|
|
97
|
|
|
|
|
|
|
|
98
|
3
|
|
|
3
|
|
1698
|
use Bio::SeqFeature::Generic; |
|
3
|
|
|
|
|
5
|
|
|
3
|
|
|
|
|
78
|
|
99
|
|
|
|
|
|
|
|
100
|
|
|
|
|
|
|
|
101
|
3
|
|
|
3
|
|
16
|
use base qw(Bio::Root::Root); |
|
3
|
|
|
|
|
3
|
|
|
3
|
|
|
|
|
6314
|
|
102
|
|
|
|
|
|
|
|
103
|
|
|
|
|
|
|
my $fac = Bio::Phylo::Factory->new(); |
104
|
|
|
|
|
|
|
|
105
|
|
|
|
|
|
|
|
106
|
|
|
|
|
|
|
=head2 new |
107
|
|
|
|
|
|
|
|
108
|
|
|
|
|
|
|
Title : new |
109
|
|
|
|
|
|
|
Usage : my $obj = Bio::Nexml::Factory->new(); |
110
|
|
|
|
|
|
|
Function: Builds a new L object |
111
|
|
|
|
|
|
|
Returns : L object |
112
|
|
|
|
|
|
|
Args : none |
113
|
|
|
|
|
|
|
|
114
|
|
|
|
|
|
|
=cut |
115
|
|
|
|
|
|
|
|
116
|
|
|
|
|
|
|
sub new { |
117
|
1
|
|
|
1
|
1
|
3
|
my($class,@args) = @_; |
118
|
1
|
|
|
|
|
10
|
my $self = $class->SUPER::new(@args); |
119
|
|
|
|
|
|
|
} |
120
|
|
|
|
|
|
|
|
121
|
|
|
|
|
|
|
#should all these creates be private methods? |
122
|
|
|
|
|
|
|
# naah./maj |
123
|
|
|
|
|
|
|
|
124
|
|
|
|
|
|
|
=head2 create_bperl_aln |
125
|
|
|
|
|
|
|
|
126
|
|
|
|
|
|
|
Title : create_bperl_aln |
127
|
|
|
|
|
|
|
Usage : my @alns = $factory->create_bperl_aln($objIO); |
128
|
|
|
|
|
|
|
Function: Converts Bio::Phylo::Matrices::Matrix objects into L objects |
129
|
|
|
|
|
|
|
Returns : an array of L objects |
130
|
|
|
|
|
|
|
Args : Bio::NexmlIO, Bio::SeqIO, Bio::AlignIO, or Bio::TreeIO |
131
|
|
|
|
|
|
|
|
132
|
|
|
|
|
|
|
see [http://search.cpan.org/~rvosa/Bio-Phylo/lib/Bio/Phylo/Project.pm Bio::Phylo::Project] |
133
|
|
|
|
|
|
|
|
134
|
|
|
|
|
|
|
=cut |
135
|
|
|
|
|
|
|
|
136
|
|
|
|
|
|
|
sub create_bperl_aln { |
137
|
0
|
|
|
0
|
1
|
|
my ($self, $caller) = @_; |
138
|
0
|
|
|
|
|
|
my ($start, $end, $seq, $desc); |
139
|
0
|
|
|
|
|
|
my $matrices = $caller->doc->get_matrices(); |
140
|
0
|
|
|
|
|
|
my @alns; |
141
|
|
|
|
|
|
|
|
142
|
0
|
|
|
|
|
|
foreach my $matrix (@$matrices) |
143
|
|
|
|
|
|
|
{ |
144
|
|
|
|
|
|
|
#check if mol_type is something that makes sense to be an aln |
145
|
0
|
|
|
|
|
|
my $mol_type = lc($matrix->get_type()); |
146
|
0
|
0
|
0
|
|
|
|
unless ($mol_type eq 'dna' || $mol_type eq 'rna' || $mol_type eq 'protein') |
|
|
|
0
|
|
|
|
|
147
|
|
|
|
|
|
|
{ |
148
|
0
|
|
|
|
|
|
next; |
149
|
|
|
|
|
|
|
# something for the back-burner: BioPerl has objects |
150
|
|
|
|
|
|
|
# to handle arbitrary genotypes; might be cool to |
151
|
|
|
|
|
|
|
# be able to create something besides alignments |
152
|
|
|
|
|
|
|
# here .../maj |
153
|
|
|
|
|
|
|
} |
154
|
|
|
|
|
|
|
|
155
|
|
|
|
|
|
|
#continue creating an aln |
156
|
0
|
|
|
|
|
|
my $aln = Bio::SimpleAlign->new(); |
157
|
0
|
|
|
|
|
|
my $taxa = $matrix->get_taxa(); |
158
|
|
|
|
|
|
|
|
159
|
|
|
|
|
|
|
# TODO: should $caller->{_ID} always be defined? |
160
|
|
|
|
|
|
|
# ATM, this is a Bio::AlignIO::nexml stream... |
161
|
0
|
0
|
|
|
|
|
$aln->{_Nexml_ID} = $caller->{_ID}? $caller->{_ID} . $taxa->get_xml_id : $taxa->get_xml_id; |
162
|
|
|
|
|
|
|
|
163
|
0
|
|
|
|
|
|
my $aln_feats = Bio::SeqFeature::Generic->new(); |
164
|
0
|
|
|
|
|
|
$aln_feats->add_tag_value('NexmlIO_ID', $caller->{_ID}); |
165
|
|
|
|
|
|
|
#check if there is a taxa associated with this alignment |
166
|
0
|
0
|
|
|
|
|
if ($taxa) { |
167
|
0
|
|
|
|
|
|
$aln_feats->add_tag_value('taxa_id', $taxa->get_xml_id()); |
168
|
0
|
0
|
|
|
|
|
$aln_feats->add_tag_value('taxa_label', $taxa->get_name()) if $taxa->get_name(); |
169
|
|
|
|
|
|
|
|
170
|
0
|
|
|
|
|
|
my $taxon = $taxa->first; |
171
|
0
|
|
|
|
|
|
while ($taxon) { |
172
|
0
|
|
|
|
|
|
$aln_feats->add_tag_value('taxon', $taxon->get_name); |
173
|
0
|
|
|
|
|
|
$taxon = $taxa->next; |
174
|
|
|
|
|
|
|
} |
175
|
|
|
|
|
|
|
} |
176
|
0
|
|
|
|
|
|
$aln->add_SeqFeature($aln_feats); |
177
|
|
|
|
|
|
|
|
178
|
0
|
|
|
|
|
|
my $basename = $matrix->get_name(); |
179
|
0
|
|
|
|
|
|
$aln->id($basename); |
180
|
0
|
|
|
|
|
|
my $seqNum = 0; |
181
|
0
|
|
|
|
|
|
my$row = $matrix->first; |
182
|
0
|
|
|
|
|
|
while ($row) |
183
|
|
|
|
|
|
|
{ |
184
|
0
|
|
|
|
|
|
my $newSeq = $row->get_char(); |
185
|
0
|
|
|
|
|
|
my $rowlabel; |
186
|
0
|
|
|
|
|
|
$seqNum++; |
187
|
|
|
|
|
|
|
|
188
|
|
|
|
|
|
|
#constuct seqID based on matrix label and row id |
189
|
0
|
|
|
|
|
|
my $seqID = "$basename.row_$seqNum"; |
190
|
|
|
|
|
|
|
|
191
|
|
|
|
|
|
|
#Check if theres a row label and if not default to seqID |
192
|
0
|
0
|
|
|
|
|
if( !defined($rowlabel = $row->get_name())) {$rowlabel = $seqID;} |
|
0
|
|
|
|
|
|
|
193
|
|
|
|
|
|
|
|
194
|
0
|
|
|
|
|
|
$seq = Bio::LocatableSeq->new( |
195
|
|
|
|
|
|
|
-seq => $newSeq, |
196
|
|
|
|
|
|
|
-display_id => "$rowlabel", |
197
|
|
|
|
|
|
|
#-description => $desc, |
198
|
|
|
|
|
|
|
-alphabet => $mol_type, |
199
|
|
|
|
|
|
|
); |
200
|
0
|
|
|
|
|
|
my $seq_feats; |
201
|
|
|
|
|
|
|
#check if there is a taxa associated w/ this alignment |
202
|
0
|
0
|
|
|
|
|
if($taxa) |
203
|
|
|
|
|
|
|
{ |
204
|
0
|
0
|
|
|
|
|
if (my $taxon = $taxa->get_by_name($row->get_taxon->get_name())) { |
205
|
|
|
|
|
|
|
#attach taxon to each sequence by using the sequenceID because |
206
|
|
|
|
|
|
|
#LocatableSeq does not support features |
207
|
0
|
|
|
|
|
|
my $taxon_name = $taxon->get_name(); |
208
|
0
|
|
|
|
|
|
$seq_feats = Bio::SeqFeature::Generic->new(); |
209
|
0
|
|
|
|
|
|
$seq_feats->add_tag_value('taxon', "$taxon_name"); |
210
|
0
|
|
|
|
|
|
$seq_feats->add_tag_value('id', "$rowlabel"); |
211
|
|
|
|
|
|
|
} |
212
|
|
|
|
|
|
|
} |
213
|
0
|
|
|
|
|
|
$aln->add_seq($seq); |
214
|
0
|
|
|
|
|
|
$aln->add_SeqFeature($seq_feats); |
215
|
0
|
|
|
|
|
|
$self->debug("Reading r$rowlabel\n"); |
216
|
|
|
|
|
|
|
|
217
|
0
|
|
|
|
|
|
$row = $matrix->next(); |
218
|
|
|
|
|
|
|
} |
219
|
0
|
|
|
|
|
|
push (@alns, $aln); |
220
|
|
|
|
|
|
|
} |
221
|
0
|
|
|
|
|
|
return \@alns; |
222
|
|
|
|
|
|
|
} |
223
|
|
|
|
|
|
|
|
224
|
|
|
|
|
|
|
|
225
|
|
|
|
|
|
|
=head2 create_bperl_tree |
226
|
|
|
|
|
|
|
|
227
|
|
|
|
|
|
|
Title : create_bperl_tree |
228
|
|
|
|
|
|
|
Usage : my @trees = $factory->create_bperl_seq($objIO); |
229
|
|
|
|
|
|
|
Function: Converts Bio::Phylo::Forest::Tree objects into L objects |
230
|
|
|
|
|
|
|
Returns : an array of L objects |
231
|
|
|
|
|
|
|
Args : Bio::NexmlIO, Bio::SeqIO, Bio::AlignIO, or Bio::TreeIO |
232
|
|
|
|
|
|
|
|
233
|
|
|
|
|
|
|
see [http://search.cpan.org/~rvosa/Bio-Phylo/lib/Bio/Phylo/Project.pm Bio::Phylo::Project] |
234
|
|
|
|
|
|
|
|
235
|
|
|
|
|
|
|
=cut |
236
|
|
|
|
|
|
|
|
237
|
|
|
|
|
|
|
sub create_bperl_tree { |
238
|
0
|
|
|
0
|
1
|
|
my($self, $caller) = @_; |
239
|
0
|
|
|
|
|
|
my @trees; |
240
|
|
|
|
|
|
|
|
241
|
0
|
|
|
|
|
|
my $forests = $caller->doc->get_forests(); |
242
|
|
|
|
|
|
|
|
243
|
0
|
|
|
|
|
|
foreach my $forest (@$forests) |
244
|
|
|
|
|
|
|
{ |
245
|
0
|
|
0
|
|
|
|
my $basename = $forest->get_name() || ''; |
246
|
0
|
|
|
|
|
|
my $taxa = $forest->get_taxa(); |
247
|
0
|
|
|
|
|
|
my $taxa_label = $taxa->get_name(); |
248
|
0
|
|
|
|
|
|
my $taxa_id = $taxa->get_xml_id(); |
249
|
|
|
|
|
|
|
|
250
|
0
|
|
|
|
|
|
my $t = $forest->first(); |
251
|
|
|
|
|
|
|
|
252
|
0
|
|
|
|
|
|
while ($t) |
253
|
|
|
|
|
|
|
{ |
254
|
0
|
|
|
|
|
|
my %created_nodes; |
255
|
0
|
|
|
|
|
|
my $tree_id = $t->get_name(); |
256
|
0
|
|
|
|
|
|
my $tree = Bio::Tree::Tree->new(-id => "$basename.$tree_id"); |
257
|
|
|
|
|
|
|
|
258
|
|
|
|
|
|
|
#set the taxa info of the tree |
259
|
0
|
0
|
|
|
|
|
$tree->add_tag_value('taxa_label', $taxa_label) if defined($taxa_label); |
260
|
0
|
0
|
|
|
|
|
$tree->add_tag_value('taxa_id', $taxa_id) if defined($taxa_id); |
261
|
|
|
|
|
|
|
|
262
|
|
|
|
|
|
|
# TODO: should $caller->{_ID} always be defined? |
263
|
|
|
|
|
|
|
# ATM, this is a Bio::TreeIO::nexml stream... |
264
|
0
|
0
|
|
|
|
|
$tree->add_tag_value('_NexmlIO_ID', $caller->{_ID}) if $caller->{_ID}; |
265
|
|
|
|
|
|
|
|
266
|
0
|
|
|
|
|
|
my $taxon = $taxa->first; |
267
|
0
|
|
|
|
|
|
while($taxon) { |
268
|
0
|
0
|
|
|
|
|
$tree->add_tag_value('taxon', $taxon->get_name()) if defined($taxon->get_name); |
269
|
0
|
|
|
|
|
|
$taxon = $taxa->next; |
270
|
|
|
|
|
|
|
} |
271
|
|
|
|
|
|
|
|
272
|
|
|
|
|
|
|
#process terminals only, removing terminals as they get processed |
273
|
|
|
|
|
|
|
#which inturn creates new terminals to process until the entire tree has been processed |
274
|
0
|
|
|
|
|
|
my $terminals = $t->get_terminals(); |
275
|
|
|
|
|
|
|
# for(my $i=0; $i<@$terminals; $i++) |
276
|
0
|
|
|
|
|
|
while (my $terminal = shift @$terminals) |
277
|
|
|
|
|
|
|
{ |
278
|
|
|
|
|
|
|
# my $terminal = $$terminals[$i]; |
279
|
0
|
|
|
|
|
|
my $new_node_id = $terminal->get_name(); |
280
|
0
|
|
|
|
|
|
my $newNode; |
281
|
|
|
|
|
|
|
|
282
|
0
|
0
|
|
|
|
|
if(exists $created_nodes{$new_node_id}) |
283
|
|
|
|
|
|
|
{ |
284
|
0
|
|
|
|
|
|
$newNode = $created_nodes{$new_node_id}; |
285
|
|
|
|
|
|
|
} |
286
|
|
|
|
|
|
|
else |
287
|
|
|
|
|
|
|
{ |
288
|
0
|
|
|
|
|
|
$newNode = Bio::Tree::Node->new(); |
289
|
0
|
|
0
|
|
|
|
$new_node_id ||= 'internal_'.$newNode->_creation_id; |
290
|
0
|
|
|
|
|
|
$newNode->id($new_node_id); |
291
|
|
|
|
|
|
|
|
292
|
0
|
|
|
|
|
|
$created_nodes{$new_node_id} = $newNode; |
293
|
|
|
|
|
|
|
} |
294
|
|
|
|
|
|
|
|
295
|
|
|
|
|
|
|
#check if taxa data exists for the current node ($terminal) |
296
|
0
|
0
|
|
|
|
|
if($taxa) { |
297
|
0
|
|
|
|
|
|
my $taxon = $terminal->get_taxon(); |
298
|
0
|
0
|
0
|
|
|
|
$newNode->add_tag_value("taxon", $taxon->get_name()) if $taxon && $taxon->get_name; |
299
|
|
|
|
|
|
|
} |
300
|
|
|
|
|
|
|
|
301
|
|
|
|
|
|
|
#check if you've reached the root of the tree and if so, stop. |
302
|
0
|
0
|
|
|
|
|
if($terminal->is_root()) { |
303
|
0
|
|
|
|
|
|
$tree->set_root_node($newNode); |
304
|
0
|
|
|
|
|
|
last; |
305
|
|
|
|
|
|
|
} |
306
|
|
|
|
|
|
|
|
307
|
|
|
|
|
|
|
#transfer attributes that apply to non-root only nodes |
308
|
0
|
|
|
|
|
|
$newNode->branch_length($terminal->get_branch_length()); |
309
|
|
|
|
|
|
|
|
310
|
0
|
|
|
|
|
|
my $parent = $terminal->get_parent(); |
311
|
0
|
|
|
|
|
|
my $parentID = $parent->get_name(); |
312
|
0
|
0
|
|
|
|
|
if(exists $created_nodes{$parentID}) |
313
|
|
|
|
|
|
|
{ |
314
|
|
|
|
|
|
|
|
315
|
0
|
|
|
|
|
|
$created_nodes{$parentID}->add_Descendent($newNode); |
316
|
|
|
|
|
|
|
} |
317
|
|
|
|
|
|
|
else |
318
|
|
|
|
|
|
|
{ |
319
|
0
|
|
|
|
|
|
my $parent_node = Bio::Tree::Node->new(); |
320
|
0
|
|
0
|
|
|
|
$parentID ||= 'internal_'.$parent_node->_creation_id; |
321
|
0
|
|
|
|
|
|
$parent_node->id($parentID); |
322
|
0
|
|
|
|
|
|
$parent_node->add_Descendent($newNode); |
323
|
0
|
|
|
|
|
|
$created_nodes{$parentID} = $parent_node; |
324
|
|
|
|
|
|
|
} |
325
|
|
|
|
|
|
|
#remove processed node from tree |
326
|
0
|
|
|
|
|
|
$parent->prune_child($terminal); |
327
|
|
|
|
|
|
|
|
328
|
|
|
|
|
|
|
#check if the parent of the removed node is now a terminal node and should be added for processing |
329
|
0
|
0
|
|
|
|
|
if($parent->is_terminal()) |
330
|
|
|
|
|
|
|
{ |
331
|
0
|
0
|
|
|
|
|
push(@$terminals, $terminal->get_parent()) if $terminal->get_parent; |
332
|
|
|
|
|
|
|
} |
333
|
|
|
|
|
|
|
} |
334
|
0
|
|
|
|
|
|
push @trees, $tree; |
335
|
0
|
|
|
|
|
|
$t = $forest->next(); |
336
|
|
|
|
|
|
|
} |
337
|
|
|
|
|
|
|
} |
338
|
0
|
|
|
|
|
|
return \@trees; |
339
|
|
|
|
|
|
|
} |
340
|
|
|
|
|
|
|
|
341
|
|
|
|
|
|
|
=head2 create_bperl_seq |
342
|
|
|
|
|
|
|
|
343
|
|
|
|
|
|
|
Title : create_bperl_seq |
344
|
|
|
|
|
|
|
Usage : my @seqs = $factory->create_bperl_seq($objIO); |
345
|
|
|
|
|
|
|
Function: Converts Bio::Phylo::Matrices::Datum objects into L objects |
346
|
|
|
|
|
|
|
Returns : an array of L objects |
347
|
|
|
|
|
|
|
Args : Bio::NexmlIO, Bio::SeqIO, Bio::AlignIO, or Bio::TreeIO |
348
|
|
|
|
|
|
|
|
349
|
|
|
|
|
|
|
see [http://search.cpan.org/~rvosa/Bio-Phylo/lib/Bio/Phylo/Project.pm Bio::Phylo::Project] |
350
|
|
|
|
|
|
|
|
351
|
|
|
|
|
|
|
=cut |
352
|
|
|
|
|
|
|
|
353
|
|
|
|
|
|
|
sub create_bperl_seq { |
354
|
0
|
|
|
0
|
1
|
|
my($self, $caller) = @_; |
355
|
0
|
|
|
|
|
|
my $matrices = $caller->doc->get_matrices(); |
356
|
0
|
|
|
|
|
|
my @seqs; |
357
|
|
|
|
|
|
|
|
358
|
0
|
|
|
|
|
|
foreach my $matrix (@$matrices) |
359
|
|
|
|
|
|
|
{ |
360
|
|
|
|
|
|
|
#check if mol_type is something that makes sense to be a seq |
361
|
0
|
|
|
|
|
|
my $mol_type = lc($matrix->get_type()); |
362
|
0
|
0
|
0
|
|
|
|
unless ($mol_type eq 'dna' || $mol_type eq 'rna' || $mol_type eq 'protein') |
|
|
|
0
|
|
|
|
|
363
|
|
|
|
|
|
|
{ |
364
|
0
|
|
|
|
|
|
next; |
365
|
|
|
|
|
|
|
} |
366
|
|
|
|
|
|
|
|
367
|
0
|
|
|
|
|
|
my $taxa = $matrix->get_taxa(); |
368
|
0
|
|
|
|
|
|
my $seqnum = 0; |
369
|
0
|
|
|
|
|
|
my $taxa_id = $taxa->get_xml_id(); |
370
|
0
|
|
|
|
|
|
my $taxa_label = $taxa->get_name(); |
371
|
0
|
|
|
|
|
|
my $basename = $matrix->get_name(); |
372
|
0
|
|
|
|
|
|
my $row = $matrix->first; |
373
|
0
|
|
|
|
|
|
while ($row) |
374
|
|
|
|
|
|
|
{ |
375
|
0
|
|
|
|
|
|
my $newSeq = $row->get_char(); |
376
|
0
|
|
|
|
|
|
my $feat = Bio::SeqFeature::Generic->new(); |
377
|
0
|
0
|
|
|
|
|
$feat->add_tag_value('matrix_label', $matrix->get_name()) if defined($matrix->get_name); |
378
|
0
|
|
|
|
|
|
$feat->add_tag_value('matrix_id', $matrix->get_xml_id()); |
379
|
0
|
|
|
|
|
|
$feat->add_tag_value('NexmlIO_ID', $caller->{_ID}); |
380
|
0
|
0
|
|
|
|
|
$feat->add_tag_value('taxa_id', $taxa_id) if defined($taxa_id); |
381
|
0
|
0
|
|
|
|
|
$feat->add_tag_value('taxa_label', $taxa_label) if defined($taxa_label); |
382
|
|
|
|
|
|
|
|
383
|
0
|
|
|
|
|
|
$seqnum++; |
384
|
|
|
|
|
|
|
#construct full sequence id by using bio::phylo "matrix label" and "row id" |
385
|
0
|
|
|
|
|
|
my $seqID = "$basename.seq_$seqnum"; |
386
|
0
|
|
|
|
|
|
my $rowlabel; |
387
|
|
|
|
|
|
|
#check if there is a label for the row, if not default to seqID |
388
|
0
|
0
|
|
|
|
|
if (!defined ($rowlabel = $row->get_name())) {$rowlabel = $seqID;} |
|
0
|
|
|
|
|
|
|
389
|
0
|
|
|
|
|
|
else {$seqID = $rowlabel;} |
390
|
|
|
|
|
|
|
|
391
|
|
|
|
|
|
|
#build the seq object using the factory create method |
392
|
0
|
|
|
|
|
|
my $seqbuilder = Bio::Seq::SeqFactory->new('-type' => 'Bio::Seq'); |
393
|
0
|
|
|
|
|
|
my $seq = $seqbuilder->create( |
394
|
|
|
|
|
|
|
-seq => $newSeq, |
395
|
|
|
|
|
|
|
-id => $rowlabel, |
396
|
|
|
|
|
|
|
-primary_id => $seqID, |
397
|
|
|
|
|
|
|
#-desc => $fulldesc, |
398
|
|
|
|
|
|
|
-alphabet => $mol_type, |
399
|
|
|
|
|
|
|
-direct => 1, |
400
|
|
|
|
|
|
|
); |
401
|
|
|
|
|
|
|
# TODO: should $caller->{_ID} always be defined? |
402
|
|
|
|
|
|
|
# ATM, this is a Bio::SeqIO::nexml stream... |
403
|
0
|
0
|
|
|
|
|
$seq->{_Nexml_ID} = $caller->{_ID} ? $caller->{_ID} . $taxa_id : $taxa_id; |
404
|
0
|
0
|
|
|
|
|
$seq->{_Nexml_matrix_ID} = $caller->{_ID} ? $caller->{_ID} . $matrix->get_xml_id() : $matrix->get_xml_id(); |
405
|
|
|
|
|
|
|
|
406
|
|
|
|
|
|
|
#check if taxon linked to sequence if so create feature to attach to alignment |
407
|
0
|
0
|
|
|
|
|
if ($taxa) { |
408
|
0
|
|
|
|
|
|
my $taxon = $taxa->first; |
409
|
0
|
|
|
|
|
|
while ($taxon) { |
410
|
0
|
0
|
|
|
|
|
$feat->add_tag_value('taxon', $taxon->get_name) if defined($taxon->get_name); |
411
|
0
|
0
|
|
|
|
|
if($taxon eq $row->get_taxon) { |
412
|
0
|
|
|
|
|
|
my $taxon_name = $taxon->get_name(); |
413
|
|
|
|
|
|
|
|
414
|
0
|
0
|
|
|
|
|
$feat->add_tag_value('my_taxon', "$taxon_name") if defined($taxon_name); |
415
|
0
|
|
|
|
|
|
$feat->add_tag_value('id', $rowlabel); |
416
|
|
|
|
|
|
|
} |
417
|
0
|
|
|
|
|
|
$taxon = $taxa->next; |
418
|
|
|
|
|
|
|
} |
419
|
|
|
|
|
|
|
} |
420
|
0
|
|
|
|
|
|
$seq->add_SeqFeature($feat); |
421
|
0
|
|
|
|
|
|
push (@seqs, $seq); |
422
|
|
|
|
|
|
|
|
423
|
0
|
|
|
|
|
|
$row = $matrix->next; |
424
|
|
|
|
|
|
|
} |
425
|
|
|
|
|
|
|
} |
426
|
0
|
|
|
|
|
|
return \@seqs; |
427
|
|
|
|
|
|
|
} |
428
|
|
|
|
|
|
|
|
429
|
|
|
|
|
|
|
=head2 create_bphylo_tree |
430
|
|
|
|
|
|
|
|
431
|
|
|
|
|
|
|
Title : create_bphylo_tree |
432
|
|
|
|
|
|
|
Usage : my $bphylo_tree = $factory->create_bphylo_tree($bperl_tree); |
433
|
|
|
|
|
|
|
Function: Converts a L object into Bio::Phylo::Forest::Tree object |
434
|
|
|
|
|
|
|
Returns : a Bio::Phylo::Forest::Tree object |
435
|
|
|
|
|
|
|
Args : Bio::Tree::Tree object |
436
|
|
|
|
|
|
|
|
437
|
|
|
|
|
|
|
=cut |
438
|
|
|
|
|
|
|
|
439
|
|
|
|
|
|
|
sub create_bphylo_tree { |
440
|
0
|
|
|
0
|
1
|
|
my ($self, $bptree, $taxa) = @_; |
441
|
|
|
|
|
|
|
#most of the code below ripped form Bio::Phylo::Forest::Tree::new_from_bioperl()d |
442
|
|
|
|
|
|
|
|
443
|
0
|
|
|
|
|
|
my $tree = $fac->create_tree; |
444
|
0
|
|
|
|
|
|
my $class = 'Bio::Phylo::Forest::Tree'; |
445
|
|
|
|
|
|
|
|
446
|
0
|
0
|
0
|
|
|
|
if ( ref $bptree && $bptree->isa('Bio::Tree::TreeI') ) { |
447
|
0
|
|
|
|
|
|
bless $tree, $class; |
448
|
0
|
|
|
|
|
|
($tree) = _copy_tree( $tree, $bptree->get_root_node, "", $taxa); |
449
|
|
|
|
|
|
|
|
450
|
|
|
|
|
|
|
# copy name |
451
|
0
|
|
|
|
|
|
my $name = $bptree->id; |
452
|
0
|
0
|
|
|
|
|
$tree->set_name( $name ) if defined $name; |
453
|
|
|
|
|
|
|
|
454
|
|
|
|
|
|
|
# copy score |
455
|
0
|
|
|
|
|
|
my $score = $bptree->score; |
456
|
0
|
0
|
|
|
|
|
$tree->set_score( $score ) if defined $score; |
457
|
|
|
|
|
|
|
} |
458
|
|
|
|
|
|
|
else { |
459
|
0
|
|
|
|
|
|
$self->throw('Not a bioperl tree!'); |
460
|
|
|
|
|
|
|
} |
461
|
0
|
|
|
|
|
|
return $tree; |
462
|
|
|
|
|
|
|
} |
463
|
|
|
|
|
|
|
|
464
|
|
|
|
|
|
|
|
465
|
|
|
|
|
|
|
sub _copy_tree { |
466
|
0
|
|
|
0
|
|
|
my ( $tree, $bpnode, $parent, $taxa ) = @_; |
467
|
0
|
|
|
|
|
|
my $node = create_bphylo_node($bpnode); |
468
|
0
|
|
|
|
|
|
my $taxon; |
469
|
0
|
0
|
|
|
|
|
if ($parent) { |
470
|
0
|
|
|
|
|
|
$parent->set_child($node); |
471
|
|
|
|
|
|
|
} |
472
|
0
|
0
|
|
|
|
|
if (my $bptaxon_name = $bpnode->get_tag_values('taxon')) |
473
|
|
|
|
|
|
|
{ |
474
|
0
|
|
|
|
|
|
$node->set_taxon($taxa->get_by_name($bptaxon_name)); |
475
|
|
|
|
|
|
|
} |
476
|
0
|
|
|
|
|
|
$tree->insert($node); |
477
|
0
|
|
|
|
|
|
foreach my $bpchild ( $bpnode->each_Descendent ) { |
478
|
0
|
|
|
|
|
|
_copy_tree( $tree, $bpchild, $node, $taxa ); |
479
|
|
|
|
|
|
|
} |
480
|
0
|
|
|
|
|
|
return $tree; |
481
|
|
|
|
|
|
|
} |
482
|
|
|
|
|
|
|
|
483
|
|
|
|
|
|
|
=head2 create_bphylo_node |
484
|
|
|
|
|
|
|
|
485
|
|
|
|
|
|
|
Title : create_bphylo_node |
486
|
|
|
|
|
|
|
Usage : my $bphylo_node = $factory->create_bphylo_node($bperl_node); |
487
|
|
|
|
|
|
|
Function: Converts a L object into Bio::Phylo::Forest::Node object |
488
|
|
|
|
|
|
|
Returns : a Bio::Phylo::Forest::Node object |
489
|
|
|
|
|
|
|
Args : L object |
490
|
|
|
|
|
|
|
|
491
|
|
|
|
|
|
|
=cut |
492
|
|
|
|
|
|
|
|
493
|
|
|
|
|
|
|
sub create_bphylo_node { |
494
|
0
|
|
|
0
|
1
|
|
my ($bpnode) = @_; |
495
|
0
|
|
|
|
|
|
my $node = Bio::Phylo::Forest::Node->new(); |
496
|
|
|
|
|
|
|
|
497
|
|
|
|
|
|
|
#mostly ripped from Bio::Phylo::Forest::Node->new_from_bioperl() |
498
|
|
|
|
|
|
|
# copy name |
499
|
0
|
|
|
|
|
|
my $name = $bpnode->id; |
500
|
0
|
0
|
|
|
|
|
$node->set_name( $name ) if defined $name; |
501
|
|
|
|
|
|
|
|
502
|
|
|
|
|
|
|
# copy branch length |
503
|
0
|
|
|
|
|
|
my $branch_length = $bpnode->branch_length; |
504
|
0
|
0
|
|
|
|
|
$node->set_branch_length( $branch_length ) if defined $branch_length; |
505
|
|
|
|
|
|
|
|
506
|
|
|
|
|
|
|
# copy description |
507
|
0
|
|
|
|
|
|
my $desc = $bpnode->description; |
508
|
0
|
0
|
|
|
|
|
$node->set_desc( $desc ) if defined $desc; |
509
|
|
|
|
|
|
|
|
510
|
|
|
|
|
|
|
# copy bootstrap |
511
|
0
|
|
|
|
|
|
my $bootstrap = $bpnode->bootstrap; |
512
|
0
|
0
|
0
|
|
|
|
$node->set_score( $bootstrap ) if defined $bootstrap and looks_like_number $bootstrap; |
513
|
|
|
|
|
|
|
|
514
|
|
|
|
|
|
|
# copy other tags |
515
|
0
|
|
|
|
|
|
for my $tag ( $bpnode->get_all_tags ) { |
516
|
0
|
|
|
|
|
|
my @values = $bpnode->get_tag_values( $tag ); |
517
|
0
|
|
|
|
|
|
$node->set_generic( $tag => \@values ); |
518
|
|
|
|
|
|
|
} |
519
|
0
|
|
|
|
|
|
return $node; |
520
|
|
|
|
|
|
|
} |
521
|
|
|
|
|
|
|
|
522
|
|
|
|
|
|
|
|
523
|
|
|
|
|
|
|
=head2 create_bphylo_aln |
524
|
|
|
|
|
|
|
|
525
|
|
|
|
|
|
|
Title : create_bphylo_aln |
526
|
|
|
|
|
|
|
Usage : my $bphylo_aln = $factory->create_bphylo_aln($bperl_aln); |
527
|
|
|
|
|
|
|
Function: Converts a L object into Bio::Phylo::Matrices::Matrix object |
528
|
|
|
|
|
|
|
Returns : a Bio::Phylo::Matrices::Matrix object |
529
|
|
|
|
|
|
|
Args : Bio::SimpleAlign object |
530
|
|
|
|
|
|
|
|
531
|
|
|
|
|
|
|
=cut |
532
|
|
|
|
|
|
|
|
533
|
|
|
|
|
|
|
sub create_bphylo_aln { |
534
|
|
|
|
|
|
|
|
535
|
0
|
|
|
0
|
1
|
|
my ($self, $aln, $taxa, @args) = @_; |
536
|
|
|
|
|
|
|
|
537
|
|
|
|
|
|
|
#most of the code below ripped from Bio::Phylo::Matrices::Matrix::new_from_bioperl() |
538
|
0
|
0
|
|
|
|
|
if ( $aln->isa('Bio::Align::AlignI') ) { |
539
|
0
|
|
|
|
|
|
$aln->unmatch; |
540
|
0
|
|
|
|
|
|
$aln->map_chars('\.','-'); |
541
|
0
|
|
|
|
|
|
my @seqs = $aln->each_seq; |
542
|
0
|
|
|
|
|
|
my ( $type, $missing, $gap, $matchchar ); |
543
|
0
|
0
|
|
|
|
|
if ( $seqs[0] ) { |
544
|
0
|
|
0
|
|
|
|
$type = $seqs[0]->alphabet || $seqs[0]->_guess_alphabet || 'dna'; |
545
|
|
|
|
|
|
|
} |
546
|
|
|
|
|
|
|
else { |
547
|
0
|
|
|
|
|
|
$type = 'dna'; |
548
|
|
|
|
|
|
|
} |
549
|
|
|
|
|
|
|
|
550
|
0
|
|
0
|
|
|
|
my $matrix = $fac->create_matrix( |
|
|
|
0
|
|
|
|
|
|
|
|
0
|
|
|
|
|
551
|
|
|
|
|
|
|
'-type' => $type, |
552
|
|
|
|
|
|
|
'-special_symbols' => { |
553
|
|
|
|
|
|
|
'-missing' => $aln->missing_char || '?', |
554
|
|
|
|
|
|
|
'-matchchar' => $aln->match_char || '.', |
555
|
|
|
|
|
|
|
'-gap' => $aln->gap_char || '-', |
556
|
|
|
|
|
|
|
}, |
557
|
|
|
|
|
|
|
@args |
558
|
|
|
|
|
|
|
); |
559
|
|
|
|
|
|
|
# XXX create raw getter/setter pairs for annotation, accession, consensus_meta source |
560
|
0
|
|
|
|
|
|
for my $field ( qw(description accession id annotation consensus_meta score source) ) { |
561
|
0
|
|
|
|
|
|
$matrix->$field( $aln->$field ); |
562
|
|
|
|
|
|
|
} |
563
|
0
|
|
|
|
|
|
my $to = $matrix->get_type_object; |
564
|
0
|
|
|
|
|
|
my @feats = $aln->get_all_SeqFeatures(); |
565
|
|
|
|
|
|
|
|
566
|
0
|
|
|
|
|
|
for my $seq ( @seqs ) { |
567
|
|
|
|
|
|
|
#create datum linked to taxa |
568
|
0
|
|
|
|
|
|
my $datum = create_bphylo_datum($seq, $taxa, \@feats, '-type_object' => $to); |
569
|
0
|
|
|
|
|
|
$matrix->insert($datum); |
570
|
|
|
|
|
|
|
} |
571
|
0
|
|
|
|
|
|
return $matrix; |
572
|
|
|
|
|
|
|
} |
573
|
|
|
|
|
|
|
else { |
574
|
0
|
|
|
|
|
|
$self->throw('Not a bioperl alignment!'); |
575
|
|
|
|
|
|
|
} |
576
|
|
|
|
|
|
|
} |
577
|
|
|
|
|
|
|
|
578
|
|
|
|
|
|
|
|
579
|
|
|
|
|
|
|
|
580
|
|
|
|
|
|
|
=head2 create_bphylo_seq |
581
|
|
|
|
|
|
|
|
582
|
|
|
|
|
|
|
Title : create_bphylo_seq |
583
|
|
|
|
|
|
|
Usage : my $bphylo_seq = $factory->create_bphylo_seq($bperl_seq); |
584
|
|
|
|
|
|
|
Function: Converts a L object into Bio::Phylo::Matrices::Matrix object |
585
|
|
|
|
|
|
|
Returns : a Bio::Phylo::Matrices::Matrix object |
586
|
|
|
|
|
|
|
Args : Bio::Seq object |
587
|
|
|
|
|
|
|
|
588
|
|
|
|
|
|
|
=cut |
589
|
|
|
|
|
|
|
|
590
|
|
|
|
|
|
|
sub create_bphylo_seq { |
591
|
0
|
|
|
0
|
1
|
|
my ($self, $seq, $taxa, @args) = @_; |
592
|
0
|
|
0
|
|
|
|
my $type = $seq->alphabet || $seq->_guess_alphabet || 'dna'; |
593
|
0
|
|
|
|
|
|
$type = uc($type); |
594
|
|
|
|
|
|
|
|
595
|
0
|
|
|
|
|
|
my $dat = create_bphylo_datum($seq, $taxa, '-type' => $type); |
596
|
|
|
|
|
|
|
|
597
|
|
|
|
|
|
|
# copy seq string |
598
|
0
|
|
|
|
|
|
my $seqstring = $seq->seq; |
599
|
0
|
0
|
0
|
|
|
|
if ( $seqstring and $seqstring =~ /\S/ ) { |
600
|
0
|
|
|
|
|
|
eval { $dat->set_char( $seqstring ) }; |
|
0
|
|
|
|
|
|
|
601
|
0
|
0
|
0
|
|
|
|
if ( $@ and UNIVERSAL::isa($@,'Bio::Phylo::Util::Exceptions::InvalidData') ) { |
602
|
0
|
|
|
|
|
|
$self->throw("\n\nThe BioPerl sequence object contains invalid data ($seqstring)\n"); |
603
|
|
|
|
|
|
|
} |
604
|
|
|
|
|
|
|
} |
605
|
|
|
|
|
|
|
|
606
|
|
|
|
|
|
|
# copy name |
607
|
0
|
|
|
|
|
|
my $name = $seq->display_id; |
608
|
|
|
|
|
|
|
#$dat->set_name( $name ) if defined $name; |
609
|
|
|
|
|
|
|
|
610
|
|
|
|
|
|
|
# copy desc |
611
|
0
|
|
|
|
|
|
my $desc = $seq->desc; |
612
|
0
|
0
|
|
|
|
|
$dat->set_desc( $desc ) if defined $desc; |
613
|
|
|
|
|
|
|
|
614
|
|
|
|
|
|
|
#get features from SeqFeatureI |
615
|
0
|
|
|
|
|
|
for my $field ( qw(start end strand) ) { |
616
|
0
|
0
|
|
|
|
|
$dat->$field( $seq->$field ) if $seq->can($field); |
617
|
|
|
|
|
|
|
} |
618
|
0
|
|
|
|
|
|
return $dat; |
619
|
|
|
|
|
|
|
} |
620
|
|
|
|
|
|
|
|
621
|
|
|
|
|
|
|
=head2 create_bphylo_taxa |
622
|
|
|
|
|
|
|
|
623
|
|
|
|
|
|
|
Title : create_bphylo_seq |
624
|
|
|
|
|
|
|
Usage : my $taxa = $factory->create_bphylo_taxa($bperl_obj); |
625
|
|
|
|
|
|
|
Function: creates a taxa object from the data attached to a bioperl object |
626
|
|
|
|
|
|
|
Returns : a Bio::Phylo::Taxa object |
627
|
|
|
|
|
|
|
Args : L object, or L object, or L object |
628
|
|
|
|
|
|
|
|
629
|
|
|
|
|
|
|
=cut |
630
|
|
|
|
|
|
|
|
631
|
|
|
|
|
|
|
sub create_bphylo_taxa { |
632
|
0
|
|
|
0
|
1
|
|
my $self = shift @_; |
633
|
0
|
|
|
|
|
|
my ($obj) = @_; |
634
|
|
|
|
|
|
|
|
635
|
|
|
|
|
|
|
#check if tree or aln object |
636
|
0
|
0
|
0
|
|
|
|
if ( UNIVERSAL::isa( $obj, 'Bio::Align::AlignI' ) || UNIVERSAL::isa( $obj, 'Bio::Seq')) { |
|
|
0
|
|
|
|
|
|
637
|
0
|
|
|
|
|
|
return $self->_create_bphylo_matrix_taxa(@_); |
638
|
|
|
|
|
|
|
} |
639
|
|
|
|
|
|
|
elsif ( UNIVERSAL::isa( $obj, 'Bio::Tree::TreeI' ) ) { |
640
|
0
|
|
|
|
|
|
return $self->_create_bphylo_tree_taxa(@_); |
641
|
|
|
|
|
|
|
} |
642
|
|
|
|
|
|
|
} |
643
|
|
|
|
|
|
|
|
644
|
|
|
|
|
|
|
sub _create_bphylo_tree_taxa { |
645
|
0
|
|
|
0
|
|
|
my ($self, $tree) = @_; |
646
|
|
|
|
|
|
|
|
647
|
0
|
|
|
|
|
|
my $taxa = $fac->create_taxa(); |
648
|
0
|
|
|
|
|
|
my $taxon; |
649
|
|
|
|
|
|
|
|
650
|
|
|
|
|
|
|
#check if taxa exists |
651
|
0
|
0
|
|
|
|
|
unless ($tree->has_tag('taxa_id')) { |
652
|
0
|
|
|
|
|
|
return 0; |
653
|
|
|
|
|
|
|
} |
654
|
|
|
|
|
|
|
|
655
|
|
|
|
|
|
|
#copy taxa details |
656
|
0
|
|
|
|
|
|
$taxa->set_xml_id(($tree->get_tag_values('taxa_id'))[0]); |
657
|
0
|
|
|
|
|
|
$taxa->set_name(($tree->get_tag_values('taxa_label'))[0]); |
658
|
|
|
|
|
|
|
|
659
|
0
|
|
|
|
|
|
foreach my $taxon_name ($tree->get_tag_values('taxon')) { |
660
|
|
|
|
|
|
|
|
661
|
0
|
|
|
|
|
|
$taxon = $fac->create_taxon(-name => $taxon_name); |
662
|
0
|
|
|
|
|
|
$taxa->insert($taxon); |
663
|
|
|
|
|
|
|
} |
664
|
0
|
|
|
|
|
|
return $taxa; |
665
|
|
|
|
|
|
|
} |
666
|
|
|
|
|
|
|
|
667
|
|
|
|
|
|
|
sub _create_bphylo_matrix_taxa { |
668
|
0
|
|
|
0
|
|
|
my ($self, $aln) = @_; |
669
|
|
|
|
|
|
|
|
670
|
0
|
|
|
|
|
|
my $taxa = $fac->create_taxa(); |
671
|
0
|
|
|
|
|
|
my $taxon; |
672
|
0
|
|
|
|
|
|
my @feats = $aln->get_all_SeqFeatures(); |
673
|
|
|
|
|
|
|
|
674
|
0
|
|
|
|
|
|
foreach my $feat (@feats) { |
675
|
0
|
0
|
|
|
|
|
if (my $taxa_id = ($feat->get_tag_values('taxa_id'))[0]) { |
676
|
0
|
|
|
|
|
|
my $taxa_label = ($feat->get_tag_values('taxa_label'))[0]; |
677
|
|
|
|
|
|
|
|
678
|
0
|
0
|
|
|
|
|
$taxa->set_name($taxa_label) if defined $taxa_label; |
679
|
0
|
0
|
|
|
|
|
$taxa->set_xml_id($taxa_id) if defined $taxa_label; |
680
|
0
|
|
|
|
|
|
my @taxa_bp = $feat->get_tag_values('taxon'); |
681
|
0
|
|
|
|
|
|
foreach my $taxon_name (@taxa_bp) { |
682
|
0
|
|
|
|
|
|
$taxon = $fac->create_taxon(-name => $taxon_name); |
683
|
0
|
|
|
|
|
|
$taxa->insert($taxon); |
684
|
|
|
|
|
|
|
} |
685
|
0
|
|
|
|
|
|
last; |
686
|
|
|
|
|
|
|
} |
687
|
|
|
|
|
|
|
} |
688
|
0
|
|
|
|
|
|
return $taxa |
689
|
|
|
|
|
|
|
} |
690
|
|
|
|
|
|
|
|
691
|
|
|
|
|
|
|
=head2 create_bphylo_datum |
692
|
|
|
|
|
|
|
|
693
|
|
|
|
|
|
|
Title : create_bphylo_datum |
694
|
|
|
|
|
|
|
Usage : my $bphylo_datum = $factory->create_bphylo_datum($bperl_datum); |
695
|
|
|
|
|
|
|
Function: Converts a L object into Bio::Phylo::Matrices::datum object |
696
|
|
|
|
|
|
|
Returns : a Bio::Phylo::Matrices::datum object |
697
|
|
|
|
|
|
|
Args : Bio::Seq object, Bio::Phylo::Taxa object, |
698
|
|
|
|
|
|
|
[optional] arrayref to SeqFeatures, |
699
|
|
|
|
|
|
|
[optional] key => value pairs to pass to Bio::Phylo constructor |
700
|
|
|
|
|
|
|
|
701
|
|
|
|
|
|
|
=cut |
702
|
|
|
|
|
|
|
|
703
|
|
|
|
|
|
|
sub create_bphylo_datum { |
704
|
|
|
|
|
|
|
#mostly ripped from Bio::Phylo::Matrices::Datum::new_from_bioperl() |
705
|
0
|
|
|
0
|
1
|
|
my ( $seq, $taxa, @args ) = @_; |
706
|
0
|
|
|
|
|
|
my $class = 'Bio::Phylo::Matrices::Datum'; |
707
|
0
|
|
|
|
|
|
my $feats; |
708
|
|
|
|
|
|
|
# want $seq type-check here? Allowable: is-a Bio::PrimarySeq, |
709
|
|
|
|
|
|
|
# Bio::LocatableSeq /maj |
710
|
0
|
0
|
|
|
|
|
if (@args % 2) { # odd |
711
|
0
|
|
|
|
|
|
$feats = shift @args; |
712
|
0
|
0
|
|
|
|
|
unless (ref($feats) eq 'ARRAY') { |
713
|
0
|
|
|
|
|
|
Bio::Root::Root->throw("Third argument must be array of SeqFeatures"); |
714
|
|
|
|
|
|
|
} |
715
|
|
|
|
|
|
|
} |
716
|
0
|
|
0
|
|
|
|
my $type = $seq->alphabet || $seq->_guess_alphabet || 'dna'; |
717
|
0
|
|
|
|
|
|
my $self = $class->new( '-type' => $type, @args ); |
718
|
|
|
|
|
|
|
# copy seq string |
719
|
0
|
|
|
|
|
|
my $seqstring = $seq->seq; |
720
|
0
|
0
|
0
|
|
|
|
if ( $seqstring and $seqstring =~ /\S/ ) { |
721
|
0
|
|
|
|
|
|
eval { $self->set_char( $seqstring ) }; |
|
0
|
|
|
|
|
|
|
722
|
0
|
0
|
0
|
|
|
|
if ( $@ and UNIVERSAL::isa($@,'Bio::Phylo::Util::Exceptions::InvalidData') ) { |
723
|
0
|
|
|
|
|
|
$self->throw("\n\nThe BioPerl sequence object contains invalid data ($seqstring)\n"); |
724
|
|
|
|
|
|
|
} |
725
|
|
|
|
|
|
|
} |
726
|
|
|
|
|
|
|
|
727
|
|
|
|
|
|
|
# copy name |
728
|
0
|
|
|
|
|
|
my $name = $seq->display_id; |
729
|
0
|
0
|
|
|
|
|
$self->set_name( $name ) if defined $name; |
730
|
0
|
|
|
|
|
|
my $taxon; |
731
|
0
|
0
|
|
|
|
|
my @feats = (defined $feats ? @$feats : $seq->get_all_SeqFeatures); |
732
|
|
|
|
|
|
|
# convert taxa |
733
|
0
|
|
|
|
|
|
foreach my $feat (@feats) |
734
|
|
|
|
|
|
|
{ |
735
|
|
|
|
|
|
|
#get sequence id associated with taxa to compare |
736
|
0
|
0
|
|
|
|
|
my $taxa_id = ($feat->get_tag_values('id'))[0] if $feat->has_tag('id'); |
737
|
0
|
0
|
0
|
|
|
|
if ($taxa_id && $name eq $taxa_id) |
738
|
|
|
|
|
|
|
{ |
739
|
0
|
|
|
|
|
|
my $taxon_name; |
740
|
0
|
0
|
|
|
|
|
if($feat->has_tag('my_taxon')) { |
741
|
0
|
|
|
|
|
|
$taxon_name = ($feat->get_tag_values('my_taxon'))[0] |
742
|
|
|
|
|
|
|
} |
743
|
|
|
|
|
|
|
else { |
744
|
0
|
|
|
|
|
|
$taxon_name = ($feat->get_tag_values('taxon'))[0]; |
745
|
|
|
|
|
|
|
} |
746
|
0
|
|
|
|
|
|
$self->set_taxon($taxa->get_by_name($taxon_name)); |
747
|
|
|
|
|
|
|
} |
748
|
|
|
|
|
|
|
} |
749
|
|
|
|
|
|
|
|
750
|
|
|
|
|
|
|
# copy desc |
751
|
0
|
|
|
|
|
|
my $desc = $seq->desc; |
752
|
0
|
0
|
|
|
|
|
$self->set_desc( $desc ) if defined $desc; |
753
|
|
|
|
|
|
|
|
754
|
|
|
|
|
|
|
# only Bio::LocatableSeq objs have these fields... |
755
|
0
|
|
|
|
|
|
for my $field ( qw(start end strand) ) { |
756
|
0
|
0
|
|
|
|
|
$self->$field( $seq->$field ) if $seq->can($field); |
757
|
|
|
|
|
|
|
} |
758
|
0
|
|
|
|
|
|
return $self; |
759
|
|
|
|
|
|
|
} |
760
|
|
|
|
|
|
|
|
761
|
|
|
|
|
|
|
=head2 CREATOR |
762
|
|
|
|
|
|
|
|
763
|
|
|
|
|
|
|
=cut |
764
|
|
|
|
|
|
|
|
765
|
|
|
|
|
|
|
=head1 bioperl_create |
766
|
|
|
|
|
|
|
|
767
|
|
|
|
|
|
|
Title : bioperl_create |
768
|
|
|
|
|
|
|
Usage : $bioperl_obj = $fac->bioperl_create($obj_type, $biophylo_proj); |
769
|
|
|
|
|
|
|
Function: Create a specified bioperl object using a Bio::Phylo project |
770
|
|
|
|
|
|
|
Args : scalar string ('aln', 'tree', 'seq') type designator |
771
|
|
|
|
|
|
|
Bio::Phylo::Project object |
772
|
|
|
|
|
|
|
Returns : Appropriate BioPerl object |
773
|
|
|
|
|
|
|
|
774
|
|
|
|
|
|
|
=cut |
775
|
|
|
|
|
|
|
|
776
|
|
|
|
|
|
|
sub bioperl_create { |
777
|
0
|
|
|
0
|
0
|
|
my $self = shift; |
778
|
0
|
|
|
|
|
|
my ($type, @args) = @_; |
779
|
0
|
0
|
|
|
|
|
unless (grep /^type/,qw( seq aln tree )) { |
780
|
0
|
|
|
|
|
|
$self->throw("Unrecognized type for argument 1"); |
781
|
|
|
|
|
|
|
} |
782
|
0
|
|
|
|
|
|
my $call = 'create_bioperl_'.$type; |
783
|
0
|
|
|
|
|
|
return $self->$call(@args); |
784
|
|
|
|
|
|
|
} |
785
|
|
|
|
|
|
|
|
786
|
|
|
|
|
|
|
1; |
787
|
|
|
|
|
|
|
|