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# BioPerl module for Bio::Seq::RichSeq |
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# Please direct questions and support issues to |
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# |
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# Cared for by Ewan Birney |
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# |
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# Copyright Ewan Birney |
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# |
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# You may distribute this module under the same terms as perl itself |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::Seq::RichSeq - Module implementing a sequence created from a rich |
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sequence database entry |
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=head1 SYNOPSIS |
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See L and documentation of methods. |
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=head1 DESCRIPTION |
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This module implements Bio::Seq::RichSeqI, an interface for sequences |
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created from or created for entries from/of rich sequence databanks, |
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like EMBL, GenBank, and SwissProt. Methods added to the Bio::SeqI |
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interface therefore focus on databank-specific information. Note that |
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not every rich databank format may use all of the properties provided. |
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For more information, please see the relevant |
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=head1 Implemented Interfaces |
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This class implementes the following interfaces. |
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=over 4 |
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=item L |
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Note that this includes implementing L and L, |
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specifically via L and L. Please review the |
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documentation for those modules on implementation details relevant to those |
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interfaces, as well as the ones below. |
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=item L |
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=item L |
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=item L |
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=back |
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=head1 FEEDBACK |
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=head2 Mailing Lists |
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User feedback is an integral part of the evolution of this |
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and other Bioperl modules. Send your comments and suggestions preferably |
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to one of the Bioperl mailing lists. |
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Your participation is much appreciated. |
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bioperl-l@bioperl.org - General discussion |
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http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
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=head2 Support |
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Please direct usage questions or support issues to the mailing list: |
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I |
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rather than to the module maintainer directly. Many experienced and |
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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=head2 Reporting Bugs |
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Report bugs to the Bioperl bug tracking system to help us keep track |
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the bugs and their resolution. Bug reports can be submitted via the |
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web: |
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https://github.com/bioperl/bioperl-live/issues |
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=head1 AUTHOR - Ewan Birney |
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Email birney@ebi.ac.uk |
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=head1 APPENDIX |
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The rest of the documentation details each of the object |
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methods. Internal methods are usually preceded with a _ |
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=cut |
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# Let the code begin... |
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package Bio::Seq::RichSeq; |
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use vars qw($AUTOLOAD); |
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use strict; |
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use base qw(Bio::Seq Bio::Seq::RichSeqI); |
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=head2 new |
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Title : new |
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Usage : $seq = Bio::Seq::RichSeq->new( -seq => 'ATGGGGGTGGTGGTACCCT', |
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-id => 'human_id', |
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-accession_number => 'AL000012', |
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); |
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Function: Returns a new seq object from |
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basic constructors, being a string for the sequence |
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and strings for id and accession_number |
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Returns : a new Bio::Seq::RichSeq object |
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=cut |
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sub new { |
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# standard new call.. |
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my($caller,@args) = @_; |
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my $self = $caller->SUPER::new(@args); |
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$self->{'_dates'} = []; |
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$self->{'_secondary_accession'} = []; |
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my ($dates, $xtra, $sv, |
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$keywords, $pid, $mol, |
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$division ) = $self->_rearrange([qw(DATES |
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SECONDARY_ACCESSIONS |
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SEQ_VERSION |
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KEYWORDS |
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PID |
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MOLECULE |
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DIVISION |
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)], |
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@args); |
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defined $division && $self->division($division); |
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defined $mol && $self->molecule($mol); |
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if(defined($keywords)) { |
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if(ref($keywords) && (ref($keywords) eq "ARRAY")) { |
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$self->add_keyword(@$keywords); |
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} else { |
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# got a string - use the old API |
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$self->keywords($keywords); |
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} |
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} |
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defined $sv && $self->seq_version($sv); |
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defined $pid && $self->pid($pid); |
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if( defined $dates ) { |
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if( ref($dates) eq "ARRAY" ) { |
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foreach ( @$dates) { |
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$self->add_date($_); |
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} |
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} else { |
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$self->add_date($dates); |
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} |
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} |
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if( defined $xtra ) { |
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if( ref($xtra) eq "ARRAY" ) { |
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foreach ( @$xtra) { |
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$self->add_secondary_accession($_); |
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} |
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} else { |
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$self->add_secondary_accession($xtra); |
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} |
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} |
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return $self; |
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} |
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=head2 division |
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Title : division |
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Usage : $obj->division($newval) |
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Function: |
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Returns : value of division |
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Args : newvalue (optional) |
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=cut |
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sub division { |
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my $obj = shift; |
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if( @_ ) { |
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my $value = shift; |
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$obj->{'_division'} = $value; |
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} |
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return $obj->{'_division'}; |
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} |
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=head2 molecule |
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Title : molecule |
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Usage : $obj->molecule($newval) |
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Function: |
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Returns : type of molecule (DNA, mRNA) |
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Args : newvalue (optional) |
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=cut |
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sub molecule { |
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my $obj = shift; |
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if( @_ ) { |
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my $value = shift; |
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$obj->{'_molecule'} = $value; |
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} |
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return $obj->{'_molecule'}; |
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} |
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222
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=head2 add_date |
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224
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Title : add_date |
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Usage : $self->add_date($datestr) |
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Function: adds one or more dates |
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228
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This implementation stores dates as keyed annotation, the |
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key being 'date_changed'. You can take advantage of this |
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fact when accessing the annotation collection directly. |
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Example : |
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Returns : |
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Args : a date string or an array of such strings |
235
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=cut |
238
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239
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sub add_date { |
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return shift->_add_annotation_value('date_changed',@_); |
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} |
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=head2 get_dates |
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Title : get_dates |
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Usage : my @dates = $seq->get_dates; |
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Function: Get the dates of the sequence (usually, when it was created and |
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changed. |
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Returns : an array of date strings |
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Args : |
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=cut |
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sub get_dates{ |
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return shift->_get_annotation_values('date_changed'); |
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} |
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=head2 pid |
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Title : pid |
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Usage : my $pid = $seq->pid(); |
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Function: Get (and set, depending on the implementation) the PID property |
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for the sequence. |
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Returns : a string |
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Args : |
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=cut |
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sub pid{ |
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my $self = shift; |
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return $self->{'_pid'} = shift if @_; |
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return $self->{'_pid'}; |
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} |
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=head2 accession |
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282
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Title : accession |
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Usage : $obj->accession($newval) |
284
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Function: Whilst the underlying sequence object does not |
285
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have an accession, so we need one here. |
286
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287
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In this implementation this is merely a synonym for |
288
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accession_number(). |
289
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Example : |
290
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Returns : value of accession |
291
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Args : newvalue (optional) |
292
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293
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294
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=cut |
295
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296
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sub accession { |
297
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7
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1
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605
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my ($obj,@args) = @_; |
298
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7
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34
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return $obj->accession_number(@args); |
299
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} |
300
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301
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=head2 add_secondary_accession |
302
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303
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Title : add_secondary_accession |
304
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Usage : $self->add_domment($ref) |
305
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Function: adds a secondary_accession |
306
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307
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This implementation stores secondary accession numbers as |
308
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keyed annotation, the key being 'secondary_accession'. You |
309
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can take advantage of this fact when accessing the |
310
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annotation collection directly. |
311
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312
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Example : |
313
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Returns : |
314
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Args : a string or an array of strings |
315
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316
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317
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=cut |
318
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319
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sub add_secondary_accession { |
320
|
136
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136
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1
|
265
|
return shift->_add_annotation_value('secondary_accession',@_); |
321
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} |
322
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323
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=head2 get_secondary_accessions |
324
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325
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|
|
Title : get_secondary_accessions |
326
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Usage : my @acc = $seq->get_secondary_accessions(); |
327
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|
Function: Get the secondary accession numbers as strings. |
328
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|
Returns : An array of strings |
329
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Args : none |
330
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331
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332
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=cut |
333
|
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334
|
|
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|
|
sub get_secondary_accessions{ |
335
|
40
|
|
|
40
|
1
|
130
|
return shift->_get_annotation_values('secondary_accession'); |
336
|
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|
|
} |
337
|
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338
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|
=head2 seq_version |
339
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340
|
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|
|
Title : seq_version |
341
|
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|
|
Usage : $obj->seq_version($newval) |
342
|
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|
|
|
Function: Get/set the sequence version |
343
|
|
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|
|
|
|
Returns : value of seq_version (a scalar) |
344
|
|
|
|
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|
|
Args : on set, new value (a scalar or undef, optional) |
345
|
|
|
|
|
|
|
Note : this differs from Bio::PrimarySeq version() in that this explicitly |
346
|
|
|
|
|
|
|
refers to the sequence record version one would find in a typical |
347
|
|
|
|
|
|
|
sequence file. |
348
|
|
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|
|
|
|
349
|
|
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|
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|
|
=cut |
350
|
|
|
|
|
|
|
|
351
|
|
|
|
|
|
|
sub seq_version{ |
352
|
293
|
|
|
293
|
1
|
766
|
my $self = shift; |
353
|
|
|
|
|
|
|
|
354
|
293
|
100
|
|
|
|
825
|
return $self->{'_seq_version'} = shift if @_; |
355
|
61
|
|
|
|
|
268
|
return $self->{'_seq_version'}; |
356
|
|
|
|
|
|
|
} |
357
|
|
|
|
|
|
|
|
358
|
|
|
|
|
|
|
|
359
|
|
|
|
|
|
|
=head2 add_keyword |
360
|
|
|
|
|
|
|
|
361
|
|
|
|
|
|
|
Title : add_keyword |
362
|
|
|
|
|
|
|
Usage : $obj->add_keyword($newval) |
363
|
|
|
|
|
|
|
Function: Add a new keyword to the annotation of the sequence. |
364
|
|
|
|
|
|
|
|
365
|
|
|
|
|
|
|
This implementation stores keywords as keyed annotation, |
366
|
|
|
|
|
|
|
the key being 'keyword'. You can take advantage of this |
367
|
|
|
|
|
|
|
fact when accessing the annotation collection directly. |
368
|
|
|
|
|
|
|
|
369
|
|
|
|
|
|
|
Returns : |
370
|
|
|
|
|
|
|
Args : value to be added (optional) (a string) |
371
|
|
|
|
|
|
|
|
372
|
|
|
|
|
|
|
|
373
|
|
|
|
|
|
|
=cut |
374
|
|
|
|
|
|
|
|
375
|
|
|
|
|
|
|
sub add_keyword { |
376
|
211
|
|
|
211
|
1
|
711
|
return shift->_add_annotation_value('keyword',@_); |
377
|
|
|
|
|
|
|
} |
378
|
|
|
|
|
|
|
|
379
|
|
|
|
|
|
|
=head2 get_keywords |
380
|
|
|
|
|
|
|
|
381
|
|
|
|
|
|
|
Title : get_keywords |
382
|
|
|
|
|
|
|
Usage : $obj->get_keywords($newval) |
383
|
|
|
|
|
|
|
Function: Get the keywords for this sequence as an array of strings. |
384
|
|
|
|
|
|
|
Returns : an array of strings |
385
|
|
|
|
|
|
|
Args : |
386
|
|
|
|
|
|
|
|
387
|
|
|
|
|
|
|
|
388
|
|
|
|
|
|
|
=cut |
389
|
|
|
|
|
|
|
|
390
|
|
|
|
|
|
|
sub get_keywords { |
391
|
42
|
|
|
42
|
1
|
139
|
return shift->_get_annotation_values('keyword'); |
392
|
|
|
|
|
|
|
} |
393
|
|
|
|
|
|
|
|
394
|
|
|
|
|
|
|
=head1 Private methods and synonyms for backward compatibility |
395
|
|
|
|
|
|
|
|
396
|
|
|
|
|
|
|
=cut |
397
|
|
|
|
|
|
|
|
398
|
|
|
|
|
|
|
=head2 _add_annotation_value |
399
|
|
|
|
|
|
|
|
400
|
|
|
|
|
|
|
Title : _add_annotation_value |
401
|
|
|
|
|
|
|
Usage : |
402
|
|
|
|
|
|
|
Function: Adds a value to the annotation collection under the specified |
403
|
|
|
|
|
|
|
key. Note that this is not a public method. |
404
|
|
|
|
|
|
|
Returns : |
405
|
|
|
|
|
|
|
Args : key (a string), value(s) (one or more scalars) |
406
|
|
|
|
|
|
|
|
407
|
|
|
|
|
|
|
|
408
|
|
|
|
|
|
|
=cut |
409
|
|
|
|
|
|
|
|
410
|
|
|
|
|
|
|
sub _add_annotation_value{ |
411
|
670
|
|
|
670
|
|
612
|
my $self = shift; |
412
|
670
|
|
|
|
|
677
|
my $key = shift; |
413
|
|
|
|
|
|
|
|
414
|
670
|
|
|
|
|
882
|
foreach my $val (@_) { |
415
|
1471
|
|
|
|
|
2814
|
$self->annotation->add_Annotation( |
416
|
|
|
|
|
|
|
Bio::Annotation::SimpleValue->new(-tagname => $key, |
417
|
|
|
|
|
|
|
-value => $val) |
418
|
|
|
|
|
|
|
); |
419
|
|
|
|
|
|
|
} |
420
|
|
|
|
|
|
|
} |
421
|
|
|
|
|
|
|
|
422
|
|
|
|
|
|
|
=head2 _get_annotation_values |
423
|
|
|
|
|
|
|
|
424
|
|
|
|
|
|
|
Title : _get_annotation_values |
425
|
|
|
|
|
|
|
Usage : |
426
|
|
|
|
|
|
|
Function: Gets the values of a specific annotation as identified by the |
427
|
|
|
|
|
|
|
key from the annotation collection. Note that this is not a |
428
|
|
|
|
|
|
|
public method. |
429
|
|
|
|
|
|
|
Example : |
430
|
|
|
|
|
|
|
Returns : an array of strings |
431
|
|
|
|
|
|
|
Args : the key (a string) |
432
|
|
|
|
|
|
|
|
433
|
|
|
|
|
|
|
|
434
|
|
|
|
|
|
|
=cut |
435
|
|
|
|
|
|
|
|
436
|
|
|
|
|
|
|
sub _get_annotation_values{ |
437
|
148
|
|
|
148
|
|
214
|
my $self = shift; |
438
|
|
|
|
|
|
|
|
439
|
148
|
|
|
|
|
405
|
return map { $_->value(); } $self->annotation->get_Annotations(shift); |
|
465
|
|
|
|
|
656
|
|
440
|
|
|
|
|
|
|
} |
441
|
|
|
|
|
|
|
|
442
|
|
|
|
|
|
|
# |
443
|
|
|
|
|
|
|
## |
444
|
|
|
|
|
|
|
### Deprecated methods kept for ease of transition |
445
|
|
|
|
|
|
|
## |
446
|
|
|
|
|
|
|
# |
447
|
|
|
|
|
|
|
|
448
|
|
|
|
|
|
|
sub keywords { |
449
|
39
|
|
|
39
|
0
|
75
|
my $self = shift; |
450
|
|
|
|
|
|
|
|
451
|
|
|
|
|
|
|
# have we been called in set mode? |
452
|
39
|
100
|
|
|
|
119
|
if(@_) { |
453
|
|
|
|
|
|
|
# yes; translate to the new API |
454
|
1
|
|
|
|
|
2
|
foreach my $kwd (@_) { |
455
|
1
|
|
|
|
|
8
|
$self->add_keyword(split(/\s*;\s*/,$kwd)); |
456
|
|
|
|
|
|
|
} |
457
|
|
|
|
|
|
|
} else { |
458
|
|
|
|
|
|
|
# no; translate read-only to the new API |
459
|
38
|
|
|
|
|
136
|
return join("; ",$self->get_keywords()); |
460
|
|
|
|
|
|
|
} |
461
|
|
|
|
|
|
|
} |
462
|
|
|
|
|
|
|
|
463
|
|
|
|
|
|
|
sub each_date { |
464
|
0
|
|
|
0
|
0
|
|
my ($self) = @_; |
465
|
0
|
|
|
|
|
|
$self->warn("Deprecated method... please use get_dates"); |
466
|
0
|
|
|
|
|
|
return $self->get_dates; |
467
|
|
|
|
|
|
|
} |
468
|
|
|
|
|
|
|
|
469
|
|
|
|
|
|
|
|
470
|
|
|
|
|
|
|
sub each_secondary_accession { |
471
|
0
|
|
|
0
|
0
|
|
my ($self) = @_; |
472
|
0
|
|
|
|
|
|
$self->warn("each_secondary_accession - deprecated method. use get_secondary_accessions"); |
473
|
0
|
|
|
|
|
|
return $self->get_secondary_accessions; |
474
|
|
|
|
|
|
|
|
475
|
|
|
|
|
|
|
} |
476
|
|
|
|
|
|
|
|
477
|
|
|
|
|
|
|
sub sv { |
478
|
0
|
|
|
0
|
0
|
|
my ($obj,$value) = @_; |
479
|
0
|
|
|
|
|
|
$obj->warn("sv - deprecated method. use seq_version"); |
480
|
0
|
|
|
|
|
|
$obj->seq_version($value); |
481
|
|
|
|
|
|
|
} |
482
|
|
|
|
|
|
|
|
483
|
|
|
|
|
|
|
|
484
|
|
|
|
|
|
|
1; |