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# BioPerl module for Bio::Search::Result::HMMERResult |
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# |
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# Please direct questions and support issues to |
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# |
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# Cared for by Jason Stajich |
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# |
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# Copyright Jason Stajich |
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# |
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# You may distribute this module under the same terms as perl itself |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::Search::Result::HMMERResult - A Result object for HMMER results |
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=head1 SYNOPSIS |
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use Bio::Search::Result::HMMERResult; |
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my $result = Bio::Search::Result::HMMERResult->new |
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( -hmm_name => 'pfam', |
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-sequence_file => 'roa1.pep', |
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-hits => \@hits); |
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# generally we use Bio::SearchIO to build these objects |
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use Bio::SearchIO; |
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my $in = Bio::SearchIO->new(-format => 'hmmer', |
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-file => 'result.hmmer'); |
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while( my $result = $in->next_result ) { |
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print $result->query_name, " ", $result->algorithm, " ", $result->num_hits(), " hits\n"; |
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} |
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=head1 DESCRIPTION |
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This is a specialization of L. |
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There are a few extra methods, specifically L, |
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L, L, and L. |
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=head1 FEEDBACK |
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=head2 Mailing Lists |
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User feedback is an integral part of the evolution of this and other |
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Bioperl modules. Send your comments and suggestions preferably to |
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the Bioperl mailing list. Your participation is much appreciated. |
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bioperl-l@bioperl.org - General discussion |
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http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
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=head2 Support |
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Please direct usage questions or support issues to the mailing list: |
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I |
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rather than to the module maintainer directly. Many experienced and |
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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=head2 Reporting Bugs |
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Report bugs to the Bioperl bug tracking system to help us keep track |
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of the bugs and their resolution. Bug reports can be submitted via the |
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web: |
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https://github.com/bioperl/bioperl-live/issues |
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=head1 AUTHOR - Jason Stajich |
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Email jason@bioperl.org |
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=head1 APPENDIX |
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The rest of the documentation details each of the object methods. |
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Internal methods are usually preceded with a _ |
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=cut |
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# Let the code begin... |
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package Bio::Search::Result::HMMERResult; |
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use strict; |
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use base qw(Bio::Search::Result::GenericResult); |
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=head2 new |
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Title : new |
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Usage : my $obj = Bio::Search::Result::HMMERResult->new(); |
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Function: Builds a new Bio::Search::Result::HMMERResult object |
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Returns : Bio::Search::Result::HMMERResult |
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Args : -hmm_name => string, name of hmm file |
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-sequence_file => name of the sequence file |
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plus Bio::Search::Result::GenericResult parameters |
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-query_name => Name of query Sequence |
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-query_accession => Query accession number (if available) |
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-query_description => Description of query sequence |
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-query_length => Length of query sequence |
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-database_name => Name of database |
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-database_letters => Number of residues in database |
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-database_entries => Number of entries in database |
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-parameters => hash ref of search parameters (key => value) |
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-statistics => hash ref of search statistics (key => value) |
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-algorithm => program name (blastx) |
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-algorithm_version => version of the algorithm (2.1.2) |
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-program_reference => literature reference string for this algorithm |
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=cut |
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sub new { |
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my($class,@args) = @_; |
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my $self = $class->SUPER::new(@args); |
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my ($hmm,$seqfile) = $self->_rearrange([qw(HMM_NAME SEQUENCE_FILE)], |
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@args); |
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defined( $seqfile) && $self->sequence_file($seqfile); |
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defined( $hmm) && $self->hmm_name($hmm); |
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return $self; |
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} |
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=head2 hmm_name |
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Title : hmm_name |
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Usage : $obj->hmm_name($newval) |
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Function: Get/Set the value of hmm_name |
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Returns : value of hmm_name |
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Args : newvalue (optional) |
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=cut |
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sub hmm_name{ |
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my ($self,$value) = @_; |
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if( defined $value) { |
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$self->{'_hmm_name'} = $value; |
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} |
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return $self->{'_hmm_name'}; |
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} |
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=head2 sequence_file |
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Title : sequence_file |
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Usage : $obj->sequence_file($newval) |
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Function: Get/Set the value of sequence_file |
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Returns : value of sequence_file |
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Args : newvalue (optional) |
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=cut |
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sub sequence_file{ |
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my ($self,$value) = @_; |
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if( defined $value) { |
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$self->{'_sequence_file'} = $value; |
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} |
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return $self->{'_sequence_file'}; |
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} |
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=head2 next_model |
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Title : next_model |
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Usage : my $domain = $result->next_model |
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Function: Returns the next domain - this |
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is an alias for next_hit |
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Returns : L object |
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Args : none |
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=cut |
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sub next_model{ shift->next_hit } |
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=head2 models |
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Title : models |
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Usage : my @domains = $result->models; |
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Function: Returns the list of HMM models seen - this |
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is an alias for hits() |
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Returns : Array of L objects |
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Args : none |
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=cut |
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sub models{ shift->hits } |
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=head2 Bio::Search::Result::GenericResult inherited methods |
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=cut |
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=head2 algorithm |
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Title : algorithm |
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Usage : my $r_type = $hsp->algorithm |
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Function: Obtain the name of the algorithm used to obtain the Result |
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Returns : string (e.g., BLASTP) |
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Args : [optional] scalar string to set value |
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=cut |
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=head2 algorithm_version |
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Title : algorithm_version |
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Usage : my $r_version = $hsp->algorithm_version |
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Function: Obtain the version of the algorithm used to obtain the Result |
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Returns : string (e.g., 2.1.2) |
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Args : [optional] scalar string to set algorithm version value |
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=cut |
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=head2 Bio::Search::Result::ResultI interface methods |
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Bio::Search::Result::ResultI implementation |
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=head2 next_hit |
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Title : next_hit |
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Usage : while( $hit = $result->next_hit()) { ... } |
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Function: Returns the next available Hit object, representing potential |
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matches between the query and various entities from the database. |
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Returns : a Bio::Search::Hit::HitI object or undef if there are no more. |
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Args : none |
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=cut |
240
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241
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=head2 query_name |
242
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243
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Title : query_name |
244
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Usage : $id = $result->query_name(); |
245
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Function: Get the string identifier of the query used by the |
246
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algorithm that performed the search. |
247
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Returns : a string. |
248
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Args : [optional] new string value for query name |
249
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250
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=cut |
251
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252
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=head2 query_accession |
253
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254
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Title : query_accession |
255
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Usage : $id = $result->query_accession(); |
256
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Function: Get the accession (if available) for the query sequence |
257
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Returns : a string |
258
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Args : [optional] new string value for accession |
259
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260
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=cut |
261
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262
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=head2 query_length |
263
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264
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Title : query_length |
265
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Usage : $id = $result->query_length(); |
266
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Function: Get the length of the query sequence |
267
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used in the search. |
268
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Returns : a number |
269
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Args : [optional] new integer value for query length |
270
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271
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=cut |
272
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273
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=head2 query_description |
274
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275
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Title : query_description |
276
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Usage : $id = $result->query_description(); |
277
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Function: Get the description of the query sequence |
278
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used in the search. |
279
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Returns : a string |
280
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Args : [optional] new string for the query description |
281
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282
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=cut |
283
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284
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=head2 database_name |
285
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286
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Title : database_name |
287
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|
Usage : $name = $result->database_name() |
288
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|
Function: Used to obtain the name of the database that the query was searched |
289
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against by the algorithm. |
290
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|
Returns : a scalar string |
291
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|
|
Args : [optional] new string for the db name |
292
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293
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|
=cut |
294
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295
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|
=head2 database_letters |
296
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297
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|
Title : database_letters |
298
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|
|
Usage : $size = $result->database_letters() |
299
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|
|
Function: Used to obtain the size of database that was searched against. |
300
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|
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|
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|
|
Returns : a scalar integer (units specific to algorithm, but probably the |
301
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|
|
total number of residues in the database, if available) or undef if |
302
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|
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|
|
the information was not available to the Processor object. |
303
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|
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|
|
Args : [optional] new scalar integer for number of letters in db |
304
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305
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306
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|
|
=cut |
307
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308
|
|
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|
|
=head2 database_entries |
309
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|
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310
|
|
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|
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|
|
Title : database_entries |
311
|
|
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|
|
Usage : $num_entries = $result->database_entries() |
312
|
|
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|
|
Function: Used to obtain the number of entries contained in the database. |
313
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|
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|
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|
|
Returns : a scalar integer representing the number of entities in the database |
314
|
|
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|
|
|
|
or undef if the information was not available. |
315
|
|
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|
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|
|
Args : [optional] new integer for the number of sequence entries in the db |
316
|
|
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|
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|
|
317
|
|
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318
|
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|
=cut |
319
|
|
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|
|
320
|
|
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|
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|
|
=head2 get_parameter |
321
|
|
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|
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|
|
322
|
|
|
|
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|
|
Title : get_parameter |
323
|
|
|
|
|
|
|
Usage : my $gap_ext = $report->get_parameter('gapext') |
324
|
|
|
|
|
|
|
Function: Returns the value for a specific parameter used |
325
|
|
|
|
|
|
|
when running this report |
326
|
|
|
|
|
|
|
Returns : string |
327
|
|
|
|
|
|
|
Args : name of parameter (string) |
328
|
|
|
|
|
|
|
|
329
|
|
|
|
|
|
|
=cut |
330
|
|
|
|
|
|
|
|
331
|
|
|
|
|
|
|
=head2 available_parameters |
332
|
|
|
|
|
|
|
|
333
|
|
|
|
|
|
|
Title : available_parameters |
334
|
|
|
|
|
|
|
Usage : my @params = $report->available_paramters |
335
|
|
|
|
|
|
|
Function: Returns the names of the available parameters |
336
|
|
|
|
|
|
|
Returns : Return list of available parameters used for this report |
337
|
|
|
|
|
|
|
Args : none |
338
|
|
|
|
|
|
|
|
339
|
|
|
|
|
|
|
=cut |
340
|
|
|
|
|
|
|
|
341
|
|
|
|
|
|
|
=head2 get_statistic |
342
|
|
|
|
|
|
|
|
343
|
|
|
|
|
|
|
Title : get_statistic |
344
|
|
|
|
|
|
|
Usage : my $gap_ext = $report->get_statistic('kappa') |
345
|
|
|
|
|
|
|
Function: Returns the value for a specific statistic available |
346
|
|
|
|
|
|
|
from this report |
347
|
|
|
|
|
|
|
Returns : string |
348
|
|
|
|
|
|
|
Args : name of statistic (string) |
349
|
|
|
|
|
|
|
|
350
|
|
|
|
|
|
|
=cut |
351
|
|
|
|
|
|
|
|
352
|
|
|
|
|
|
|
=head2 available_statistics |
353
|
|
|
|
|
|
|
|
354
|
|
|
|
|
|
|
Title : available_statistics |
355
|
|
|
|
|
|
|
Usage : my @statnames = $report->available_statistics |
356
|
|
|
|
|
|
|
Function: Returns the names of the available statistics |
357
|
|
|
|
|
|
|
Returns : Return list of available statistics used for this report |
358
|
|
|
|
|
|
|
Args : none |
359
|
|
|
|
|
|
|
|
360
|
|
|
|
|
|
|
=cut |
361
|
|
|
|
|
|
|
|
362
|
|
|
|
|
|
|
=head2 Bio::Search::Result::GenericResult specific methods |
363
|
|
|
|
|
|
|
|
364
|
|
|
|
|
|
|
=cut |
365
|
|
|
|
|
|
|
|
366
|
|
|
|
|
|
|
=head2 add_hit |
367
|
|
|
|
|
|
|
|
368
|
|
|
|
|
|
|
Title : add_hit |
369
|
|
|
|
|
|
|
Usage : $report->add_hit($hit) |
370
|
|
|
|
|
|
|
Function: Adds a HitI to the stored list of hits |
371
|
|
|
|
|
|
|
Returns : Number of HitI currently stored |
372
|
|
|
|
|
|
|
Args : Bio::Search::Hit::HitI |
373
|
|
|
|
|
|
|
|
374
|
|
|
|
|
|
|
=cut |
375
|
|
|
|
|
|
|
|
376
|
|
|
|
|
|
|
=head2 rewind |
377
|
|
|
|
|
|
|
|
378
|
|
|
|
|
|
|
Title : rewind |
379
|
|
|
|
|
|
|
Usage : $result->rewind; |
380
|
|
|
|
|
|
|
Function: Allow one to reset the Hit iteration to the beginning |
381
|
|
|
|
|
|
|
Since this is an in-memory implementation |
382
|
|
|
|
|
|
|
Returns : none |
383
|
|
|
|
|
|
|
Args : none |
384
|
|
|
|
|
|
|
|
385
|
|
|
|
|
|
|
=cut |
386
|
|
|
|
|
|
|
|
387
|
|
|
|
|
|
|
sub rewind{ |
388
|
0
|
|
|
0
|
1
|
|
my ($self) = @_; |
389
|
0
|
|
|
|
|
|
$self->{'_hitindex'} = 0; |
390
|
|
|
|
|
|
|
} |
391
|
|
|
|
|
|
|
|
392
|
|
|
|
|
|
|
|
393
|
|
|
|
|
|
|
=head2 add_parameter |
394
|
|
|
|
|
|
|
|
395
|
|
|
|
|
|
|
Title : add_parameter |
396
|
|
|
|
|
|
|
Usage : $report->add_parameter('gapext', 11); |
397
|
|
|
|
|
|
|
Function: Adds a parameter |
398
|
|
|
|
|
|
|
Returns : none |
399
|
|
|
|
|
|
|
Args : key - key value name for this parama |
400
|
|
|
|
|
|
|
value - value for this parameter |
401
|
|
|
|
|
|
|
|
402
|
|
|
|
|
|
|
=cut |
403
|
|
|
|
|
|
|
|
404
|
|
|
|
|
|
|
=head2 add_statistic |
405
|
|
|
|
|
|
|
|
406
|
|
|
|
|
|
|
Title : add_statistic |
407
|
|
|
|
|
|
|
Usage : $report->add_statistic('lambda', 2.3); |
408
|
|
|
|
|
|
|
Function: Adds a parameter |
409
|
|
|
|
|
|
|
Returns : none |
410
|
|
|
|
|
|
|
Args : key - key value name for this parama |
411
|
|
|
|
|
|
|
value - value for this parameter |
412
|
|
|
|
|
|
|
|
413
|
|
|
|
|
|
|
=cut |
414
|
|
|
|
|
|
|
|
415
|
|
|
|
|
|
|
=head2 num_hits |
416
|
|
|
|
|
|
|
|
417
|
|
|
|
|
|
|
Title : num_hits |
418
|
|
|
|
|
|
|
Usage : my $hitcount= $result->num_hits |
419
|
|
|
|
|
|
|
Function: returns the number of hits for this query result |
420
|
|
|
|
|
|
|
Returns : integer |
421
|
|
|
|
|
|
|
Args : none |
422
|
|
|
|
|
|
|
|
423
|
|
|
|
|
|
|
|
424
|
|
|
|
|
|
|
=cut |
425
|
|
|
|
|
|
|
|
426
|
|
|
|
|
|
|
=head2 hits |
427
|
|
|
|
|
|
|
|
428
|
|
|
|
|
|
|
Title : hits |
429
|
|
|
|
|
|
|
Usage : my @hits = $result->hits |
430
|
|
|
|
|
|
|
Function: Returns the available hits for this Result |
431
|
|
|
|
|
|
|
Returns : Array of L objects |
432
|
|
|
|
|
|
|
Args : none |
433
|
|
|
|
|
|
|
|
434
|
|
|
|
|
|
|
|
435
|
|
|
|
|
|
|
=cut |
436
|
|
|
|
|
|
|
|
437
|
|
|
|
|
|
|
=head2 program_reference |
438
|
|
|
|
|
|
|
|
439
|
|
|
|
|
|
|
Title : program_reference |
440
|
|
|
|
|
|
|
Usage : $obj->program_reference($newval) |
441
|
|
|
|
|
|
|
Function: |
442
|
|
|
|
|
|
|
Returns : value of the literature reference for the algorithm |
443
|
|
|
|
|
|
|
Args : newvalue (optional) |
444
|
|
|
|
|
|
|
|
445
|
|
|
|
|
|
|
|
446
|
|
|
|
|
|
|
=cut |
447
|
|
|
|
|
|
|
|
448
|
|
|
|
|
|
|
1; |