line |
stmt |
bran |
cond |
sub |
pod |
time |
code |
1
|
|
|
|
|
|
|
# |
2
|
|
|
|
|
|
|
# BioPerl module for Bio::Search::Hit::HMMERHit |
3
|
|
|
|
|
|
|
# |
4
|
|
|
|
|
|
|
# Please direct questions and support issues to |
5
|
|
|
|
|
|
|
# |
6
|
|
|
|
|
|
|
# Cared for by Jason Stajich |
7
|
|
|
|
|
|
|
# |
8
|
|
|
|
|
|
|
# Copyright Jason Stajich |
9
|
|
|
|
|
|
|
# |
10
|
|
|
|
|
|
|
# You may distribute this module under the same terms as perl itself |
11
|
|
|
|
|
|
|
|
12
|
|
|
|
|
|
|
# POD documentation - main docs before the code |
13
|
|
|
|
|
|
|
|
14
|
|
|
|
|
|
|
=head1 NAME |
15
|
|
|
|
|
|
|
|
16
|
|
|
|
|
|
|
Bio::Search::Hit::HMMERHit - A Hit module for HMMER hits |
17
|
|
|
|
|
|
|
|
18
|
|
|
|
|
|
|
=head1 SYNOPSIS |
19
|
|
|
|
|
|
|
|
20
|
|
|
|
|
|
|
use Bio::Search::Hit::HMMERHit; |
21
|
|
|
|
|
|
|
my $hit = Bio::Search::Hit::HMMERHit->new(); |
22
|
|
|
|
|
|
|
# use it in the same way as Bio::Search::Hit::GenericHit |
23
|
|
|
|
|
|
|
|
24
|
|
|
|
|
|
|
=head1 DESCRIPTION |
25
|
|
|
|
|
|
|
|
26
|
|
|
|
|
|
|
This is a specialization of L. There |
27
|
|
|
|
|
|
|
are a few news methods L and L. Note that |
28
|
|
|
|
|
|
|
L and L make no sense for this object and will |
29
|
|
|
|
|
|
|
return 0. |
30
|
|
|
|
|
|
|
|
31
|
|
|
|
|
|
|
=head1 FEEDBACK |
32
|
|
|
|
|
|
|
|
33
|
|
|
|
|
|
|
=head2 Mailing Lists |
34
|
|
|
|
|
|
|
|
35
|
|
|
|
|
|
|
User feedback is an integral part of the evolution of this and other |
36
|
|
|
|
|
|
|
Bioperl modules. Send your comments and suggestions preferably to |
37
|
|
|
|
|
|
|
the Bioperl mailing list. Your participation is much appreciated. |
38
|
|
|
|
|
|
|
|
39
|
|
|
|
|
|
|
bioperl-l@bioperl.org - General discussion |
40
|
|
|
|
|
|
|
http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
41
|
|
|
|
|
|
|
|
42
|
|
|
|
|
|
|
=head2 Support |
43
|
|
|
|
|
|
|
|
44
|
|
|
|
|
|
|
Please direct usage questions or support issues to the mailing list: |
45
|
|
|
|
|
|
|
|
46
|
|
|
|
|
|
|
I |
47
|
|
|
|
|
|
|
|
48
|
|
|
|
|
|
|
rather than to the module maintainer directly. Many experienced and |
49
|
|
|
|
|
|
|
reponsive experts will be able look at the problem and quickly |
50
|
|
|
|
|
|
|
address it. Please include a thorough description of the problem |
51
|
|
|
|
|
|
|
with code and data examples if at all possible. |
52
|
|
|
|
|
|
|
|
53
|
|
|
|
|
|
|
=head2 Reporting Bugs |
54
|
|
|
|
|
|
|
|
55
|
|
|
|
|
|
|
Report bugs to the Bioperl bug tracking system to help us keep track |
56
|
|
|
|
|
|
|
of the bugs and their resolution. Bug reports can be submitted via the |
57
|
|
|
|
|
|
|
web: |
58
|
|
|
|
|
|
|
|
59
|
|
|
|
|
|
|
https://github.com/bioperl/bioperl-live/issues |
60
|
|
|
|
|
|
|
|
61
|
|
|
|
|
|
|
=head1 AUTHOR - Jason Stajich |
62
|
|
|
|
|
|
|
|
63
|
|
|
|
|
|
|
Email jason@bioperl.org |
64
|
|
|
|
|
|
|
|
65
|
|
|
|
|
|
|
=head1 APPENDIX |
66
|
|
|
|
|
|
|
|
67
|
|
|
|
|
|
|
The rest of the documentation details each of the object methods. |
68
|
|
|
|
|
|
|
Internal methods are usually preceded with a _ |
69
|
|
|
|
|
|
|
|
70
|
|
|
|
|
|
|
=cut |
71
|
|
|
|
|
|
|
|
72
|
|
|
|
|
|
|
|
73
|
|
|
|
|
|
|
# Let the code begin... |
74
|
|
|
|
|
|
|
|
75
|
|
|
|
|
|
|
|
76
|
|
|
|
|
|
|
package Bio::Search::Hit::HMMERHit; |
77
|
1
|
|
|
1
|
|
4
|
use strict; |
|
1
|
|
|
|
|
1
|
|
|
1
|
|
|
|
|
26
|
|
78
|
|
|
|
|
|
|
|
79
|
|
|
|
|
|
|
|
80
|
1
|
|
|
1
|
|
3
|
use base qw(Bio::Search::Hit::GenericHit); |
|
1
|
|
|
|
|
1
|
|
|
1
|
|
|
|
|
142
|
|
81
|
|
|
|
|
|
|
|
82
|
|
|
|
|
|
|
=head2 new |
83
|
|
|
|
|
|
|
|
84
|
|
|
|
|
|
|
Title : new |
85
|
|
|
|
|
|
|
Usage : my $obj = Bio::Search::Hit::HMMERHit->new(); |
86
|
|
|
|
|
|
|
Function: Builds a new Bio::Search::Hit::HMMERHit object |
87
|
|
|
|
|
|
|
Returns : Bio::Search::Hit::HMMERHit |
88
|
|
|
|
|
|
|
Args : |
89
|
|
|
|
|
|
|
|
90
|
|
|
|
|
|
|
Plus the Bio::Search::Hit::GenericHit inherited params |
91
|
|
|
|
|
|
|
-name => Name of Hit (required) |
92
|
|
|
|
|
|
|
-description => Description (optional) |
93
|
|
|
|
|
|
|
-accession => Accession number (optional) |
94
|
|
|
|
|
|
|
-length => Length of the Hit (optional) |
95
|
|
|
|
|
|
|
-score => Raw Score for the Hit (optional) |
96
|
|
|
|
|
|
|
-significance => Significance value for the Hit (optional) |
97
|
|
|
|
|
|
|
-algorithm => Algorithm used (BLASTP, FASTX, etc...) |
98
|
|
|
|
|
|
|
-hsps => Array ref of HSPs for this Hit. |
99
|
|
|
|
|
|
|
|
100
|
|
|
|
|
|
|
|
101
|
|
|
|
|
|
|
=cut |
102
|
|
|
|
|
|
|
|
103
|
|
|
|
|
|
|
|
104
|
|
|
|
|
|
|
=head2 next_domain |
105
|
|
|
|
|
|
|
|
106
|
|
|
|
|
|
|
Title : next_domain |
107
|
|
|
|
|
|
|
Usage : my $domain = $hit->next_domain(); |
108
|
|
|
|
|
|
|
Function: An alias for L, this will return the next HSP |
109
|
|
|
|
|
|
|
Returns : L object |
110
|
|
|
|
|
|
|
Args : none |
111
|
|
|
|
|
|
|
|
112
|
|
|
|
|
|
|
|
113
|
|
|
|
|
|
|
=cut |
114
|
|
|
|
|
|
|
|
115
|
32
|
|
|
32
|
1
|
4371
|
sub next_domain{ shift->next_hsp } |
116
|
|
|
|
|
|
|
|
117
|
|
|
|
|
|
|
=head2 domains |
118
|
|
|
|
|
|
|
|
119
|
|
|
|
|
|
|
Title : domains |
120
|
|
|
|
|
|
|
Usage : my @domains = $hit->domains(); |
121
|
|
|
|
|
|
|
Function: An alias for L, this will return the full list of hsps |
122
|
|
|
|
|
|
|
Returns : array of L objects |
123
|
|
|
|
|
|
|
Args : none |
124
|
|
|
|
|
|
|
|
125
|
|
|
|
|
|
|
|
126
|
|
|
|
|
|
|
=cut |
127
|
|
|
|
|
|
|
|
128
|
0
|
|
|
0
|
1
|
0
|
sub domains{ shift->hsps() } |
129
|
|
|
|
|
|
|
|
130
|
|
|
|
|
|
|
|
131
|
|
|
|
|
|
|
=head2 inherited Bio::Search::Hit::GenericHit methods |
132
|
|
|
|
|
|
|
|
133
|
|
|
|
|
|
|
=cut |
134
|
|
|
|
|
|
|
|
135
|
|
|
|
|
|
|
=head2 add_hsp |
136
|
|
|
|
|
|
|
|
137
|
|
|
|
|
|
|
Title : add_hsp |
138
|
|
|
|
|
|
|
Usage : $hit->add_hsp($hsp) |
139
|
|
|
|
|
|
|
Function: Add a HSP to the collection of HSPs for a Hit |
140
|
|
|
|
|
|
|
Returns : number of HSPs in the Hit |
141
|
|
|
|
|
|
|
Args : Bio::Search::HSP::HSPI object |
142
|
|
|
|
|
|
|
|
143
|
|
|
|
|
|
|
|
144
|
|
|
|
|
|
|
=cut |
145
|
|
|
|
|
|
|
|
146
|
|
|
|
|
|
|
=head2 Bio::Search::Hit::HitI methods |
147
|
|
|
|
|
|
|
|
148
|
|
|
|
|
|
|
=cut |
149
|
|
|
|
|
|
|
|
150
|
|
|
|
|
|
|
=head2 name |
151
|
|
|
|
|
|
|
|
152
|
|
|
|
|
|
|
Title : name |
153
|
|
|
|
|
|
|
Usage : $hit_name = $hit->name(); |
154
|
|
|
|
|
|
|
Function: returns the name of the Hit sequence |
155
|
|
|
|
|
|
|
Returns : a scalar string |
156
|
|
|
|
|
|
|
Args : [optional] scalar string to set the name |
157
|
|
|
|
|
|
|
|
158
|
|
|
|
|
|
|
=cut |
159
|
|
|
|
|
|
|
|
160
|
|
|
|
|
|
|
=head2 accession |
161
|
|
|
|
|
|
|
|
162
|
|
|
|
|
|
|
Title : accession |
163
|
|
|
|
|
|
|
Usage : $acc = $hit->accession(); |
164
|
|
|
|
|
|
|
Function: Retrieve the accession (if available) for the hit |
165
|
|
|
|
|
|
|
Returns : a scalar string (empty string if not set) |
166
|
|
|
|
|
|
|
Args : none |
167
|
|
|
|
|
|
|
|
168
|
|
|
|
|
|
|
=cut |
169
|
|
|
|
|
|
|
|
170
|
|
|
|
|
|
|
=head2 description |
171
|
|
|
|
|
|
|
|
172
|
|
|
|
|
|
|
Title : description |
173
|
|
|
|
|
|
|
Usage : $desc = $hit->description(); |
174
|
|
|
|
|
|
|
Function: Retrieve the description for the hit |
175
|
|
|
|
|
|
|
Returns : a scalar string |
176
|
|
|
|
|
|
|
Args : [optional] scalar string to set the descrition |
177
|
|
|
|
|
|
|
|
178
|
|
|
|
|
|
|
=cut |
179
|
|
|
|
|
|
|
|
180
|
|
|
|
|
|
|
=head2 length |
181
|
|
|
|
|
|
|
|
182
|
|
|
|
|
|
|
Title : length |
183
|
|
|
|
|
|
|
Usage : my $len = $hit->length |
184
|
|
|
|
|
|
|
Function: Returns the length of the hit |
185
|
|
|
|
|
|
|
Returns : integer |
186
|
|
|
|
|
|
|
Args : [optional] integer to set the length |
187
|
|
|
|
|
|
|
|
188
|
|
|
|
|
|
|
=cut |
189
|
|
|
|
|
|
|
|
190
|
|
|
|
|
|
|
=head2 algorithm |
191
|
|
|
|
|
|
|
|
192
|
|
|
|
|
|
|
Title : algorithm |
193
|
|
|
|
|
|
|
Usage : $alg = $hit->algorithm(); |
194
|
|
|
|
|
|
|
Function: Gets the algorithm specification that was used to obtain the hit |
195
|
|
|
|
|
|
|
For BLAST, the algorithm denotes what type of sequence was aligned |
196
|
|
|
|
|
|
|
against what (BLASTN: dna-dna, BLASTP prt-prt, BLASTX translated |
197
|
|
|
|
|
|
|
dna-prt, TBLASTN prt-translated dna, TBLASTX translated |
198
|
|
|
|
|
|
|
dna-translated dna). |
199
|
|
|
|
|
|
|
Returns : a scalar string |
200
|
|
|
|
|
|
|
Args : [optional] scalar string to set the algorithm |
201
|
|
|
|
|
|
|
|
202
|
|
|
|
|
|
|
=cut |
203
|
|
|
|
|
|
|
|
204
|
|
|
|
|
|
|
=head2 raw_score |
205
|
|
|
|
|
|
|
|
206
|
|
|
|
|
|
|
Title : raw_score |
207
|
|
|
|
|
|
|
Usage : $score = $hit->raw_score(); |
208
|
|
|
|
|
|
|
Function: Gets the "raw score" generated by the algorithm. What |
209
|
|
|
|
|
|
|
this score is exactly will vary from algorithm to algorithm, |
210
|
|
|
|
|
|
|
returning undef if unavailable. |
211
|
|
|
|
|
|
|
Returns : a scalar value |
212
|
|
|
|
|
|
|
Args : [optional] scalar value to set the raw score |
213
|
|
|
|
|
|
|
|
214
|
|
|
|
|
|
|
=cut |
215
|
|
|
|
|
|
|
|
216
|
|
|
|
|
|
|
=head2 significance |
217
|
|
|
|
|
|
|
|
218
|
|
|
|
|
|
|
Title : significance |
219
|
|
|
|
|
|
|
Usage : $significance = $hit->significance(); |
220
|
|
|
|
|
|
|
Function: Used to obtain the E or P value of a hit, i.e. the probability that |
221
|
|
|
|
|
|
|
this particular hit was obtained purely by random chance. If |
222
|
|
|
|
|
|
|
information is not available (nor calculatable from other |
223
|
|
|
|
|
|
|
information sources), return undef. |
224
|
|
|
|
|
|
|
Returns : a scalar value or undef if unavailable |
225
|
|
|
|
|
|
|
Args : [optional] scalar value to set the significance |
226
|
|
|
|
|
|
|
|
227
|
|
|
|
|
|
|
=cut |
228
|
|
|
|
|
|
|
|
229
|
|
|
|
|
|
|
=head2 bits |
230
|
|
|
|
|
|
|
|
231
|
|
|
|
|
|
|
Usage : $hit_object->bits(); |
232
|
|
|
|
|
|
|
Purpose : Gets the bit score of the best HSP for the current hit. |
233
|
|
|
|
|
|
|
Example : $bits = $hit_object->bits(); |
234
|
|
|
|
|
|
|
Returns : Integer or undef if bit score is not set |
235
|
|
|
|
|
|
|
Argument : n/a |
236
|
|
|
|
|
|
|
|
237
|
|
|
|
|
|
|
See Also : L |
238
|
|
|
|
|
|
|
|
239
|
|
|
|
|
|
|
=cut |
240
|
|
|
|
|
|
|
|
241
|
11
|
|
|
11
|
1
|
43
|
sub bits { return 0 } |
242
|
|
|
|
|
|
|
|
243
|
|
|
|
|
|
|
=head2 next_hsp |
244
|
|
|
|
|
|
|
|
245
|
|
|
|
|
|
|
Title : next_hsp |
246
|
|
|
|
|
|
|
Usage : while( $hsp = $obj->next_hsp()) { ... } |
247
|
|
|
|
|
|
|
Function : Returns the next available High Scoring Pair |
248
|
|
|
|
|
|
|
Example : |
249
|
|
|
|
|
|
|
Returns : Bio::Search::HSP::HSPI object or null if finished |
250
|
|
|
|
|
|
|
Args : none |
251
|
|
|
|
|
|
|
|
252
|
|
|
|
|
|
|
=cut |
253
|
|
|
|
|
|
|
|
254
|
|
|
|
|
|
|
=head2 hsps |
255
|
|
|
|
|
|
|
|
256
|
|
|
|
|
|
|
Usage : $hit_object->hsps(); |
257
|
|
|
|
|
|
|
Purpose : Get a list containing all HSP objects. |
258
|
|
|
|
|
|
|
: Get the numbers of HSPs for the current hit. |
259
|
|
|
|
|
|
|
Example : @hsps = $hit_object->hsps(); |
260
|
|
|
|
|
|
|
: $num = $hit_object->hsps(); # alternatively, use num_hsps() |
261
|
|
|
|
|
|
|
Returns : Array context : list of Bio::Search::HSP::BlastHSP.pm objects. |
262
|
|
|
|
|
|
|
: Scalar context: integer (number of HSPs). |
263
|
|
|
|
|
|
|
: (Equivalent to num_hsps()). |
264
|
|
|
|
|
|
|
Argument : n/a. Relies on wantarray |
265
|
|
|
|
|
|
|
Throws : Exception if the HSPs have not been collected. |
266
|
|
|
|
|
|
|
|
267
|
|
|
|
|
|
|
See Also : L, L |
268
|
|
|
|
|
|
|
|
269
|
|
|
|
|
|
|
=cut |
270
|
|
|
|
|
|
|
|
271
|
|
|
|
|
|
|
=head2 num_hsps |
272
|
|
|
|
|
|
|
|
273
|
|
|
|
|
|
|
Usage : $hit_object->num_hsps(); |
274
|
|
|
|
|
|
|
Purpose : Get the number of HSPs for the present Blast hit. |
275
|
|
|
|
|
|
|
Example : $nhsps = $hit_object->num_hsps(); |
276
|
|
|
|
|
|
|
Returns : Integer |
277
|
|
|
|
|
|
|
Argument : n/a |
278
|
|
|
|
|
|
|
Throws : Exception if the HSPs have not been collected. |
279
|
|
|
|
|
|
|
|
280
|
|
|
|
|
|
|
See Also : L |
281
|
|
|
|
|
|
|
|
282
|
|
|
|
|
|
|
=cut |
283
|
|
|
|
|
|
|
|
284
|
|
|
|
|
|
|
=head2 rewind |
285
|
|
|
|
|
|
|
|
286
|
|
|
|
|
|
|
Title : rewind |
287
|
|
|
|
|
|
|
Usage : $hit->rewind; |
288
|
|
|
|
|
|
|
Function: Allow one to reset the HSP iteration to the beginning |
289
|
|
|
|
|
|
|
Since this is an in-memory implementation |
290
|
|
|
|
|
|
|
Returns : none |
291
|
|
|
|
|
|
|
Args : none |
292
|
|
|
|
|
|
|
|
293
|
|
|
|
|
|
|
=cut |
294
|
|
|
|
|
|
|
|
295
|
|
|
|
|
|
|
=head2 iteration |
296
|
|
|
|
|
|
|
|
297
|
|
|
|
|
|
|
Title : iteration |
298
|
|
|
|
|
|
|
Usage : $obj->iteration($newval) |
299
|
|
|
|
|
|
|
Function: PSI-BLAST iteration |
300
|
|
|
|
|
|
|
Returns : value of iteration |
301
|
|
|
|
|
|
|
Args : newvalue (optional) |
302
|
|
|
|
|
|
|
|
303
|
|
|
|
|
|
|
|
304
|
|
|
|
|
|
|
=cut |
305
|
|
|
|
|
|
|
|
306
|
|
|
|
|
|
|
|
307
|
0
|
|
|
0
|
1
|
|
sub iteration { return 0 } |
308
|
|
|
|
|
|
|
|
309
|
|
|
|
|
|
|
1; |