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# BioPerl module for Bio::Search::HSP::WABAHSP |
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# |
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# Please direct questions and support issues to |
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# |
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# Cared for by Jason Stajich |
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# Copyright Jason Stajich |
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# |
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# You may distribute this module under the same terms as perl itself |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::Search::HSP::WABAHSP - HSP object suitable for describing WABA alignments |
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=head1 SYNOPSIS |
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# use this object as you would a GenericHSP |
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# a few other methods have been added including state |
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=head1 DESCRIPTION |
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This object implements a few of the extra methods such as |
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hmmstate_string which returns the HMM state representation for the |
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WABA alignment. We also must implement a method to calculate |
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homology_string since it is not returned by the algorithm in the |
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machine readable format. |
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=head1 FEEDBACK |
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=head2 Mailing Lists |
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User feedback is an integral part of the evolution of this and other |
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Bioperl modules. Send your comments and suggestions preferably to |
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the Bioperl mailing list. Your participation is much appreciated. |
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bioperl-l@bioperl.org - General discussion |
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http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
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=head2 Support |
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Please direct usage questions or support issues to the mailing list: |
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I |
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rather than to the module maintainer directly. Many experienced and |
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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=head2 Reporting Bugs |
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Report bugs to the Bioperl bug tracking system to help us keep track |
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of the bugs and their resolution. Bug reports can be submitted via the |
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web: |
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https://github.com/bioperl/bioperl-live/issues |
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=head1 AUTHOR - Jason Stajich |
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Email jason@bioperl.org |
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=head1 APPENDIX |
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The rest of the documentation details each of the object methods. |
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Internal methods are usually preceded with a _ |
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=cut |
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# Let the code begin... |
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package Bio::Search::HSP::WABAHSP; |
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use strict; |
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use Bio::Root::RootI; |
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use base qw(Bio::Search::HSP::GenericHSP); |
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=head2 new |
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Title : new |
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Usage : my $obj = Bio::Search::HSP::WABAHSP->new(); |
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Function: Builds a new Bio::Search::HSP::WABAHSP object |
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Returns : Bio::Search::HSP::WABAHSP |
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Args : -hmmstate_seq => the string representing the state output from WABA |
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=cut |
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sub new { |
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my($class,@args) = @_; |
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# gotta do some preprocessing before we send the arguments to the superclass |
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my ($len,$qs,$hs) = Bio::Root::RootI->_rearrange([qw(HSP_LENGTH |
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QUERY_SEQ |
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HIT_SEQ)],@args); |
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if( $len != length($qs) ) { |
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Bio::Root::RootI->warn("HSP_LENGTH must equal length of query_seq string, using value from QUERY_SEQ\n"); |
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$len = length($qs); |
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} |
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my( $homol_seq,$gapct,$identical) = ('',0,0); |
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for(my $i=0;$i<$len;$i++) { |
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my $q = substr($qs,$i,1); |
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my $h = substr($hs,$i,1); |
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if( $q eq '-' || $h eq '-' ) { |
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$homol_seq .= ' '; |
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$gapct ++; |
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} elsif( $q eq $h ) { |
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$homol_seq .= '|'; |
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$identical++; |
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} else { |
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$homol_seq .= ' '; |
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} |
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} |
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my $self = $class->SUPER::new('-conserved' => $identical, |
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'-identical' => $identical, |
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'-gaps' => $gapct, |
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'-homology_seq' => $homol_seq, |
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@args); |
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my ($hmmst) = $self->_rearrange([qw(HMMSTATE_SEQ)],@args); |
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defined $hmmst && $self->hmmstate_string($hmmst); |
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$self->add_tag_value('Target' , join(" ","Sequence:".$self->hit->seq_id, |
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$self->hit->start, $self->hit->end)); |
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return $self; |
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} |
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=head2 hmmstate_string |
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Title : hmmstate_string |
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Usage : my $hmmseq = $wabahsp->hmmstate_string(); |
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Function: Get/Set the WABA HMM stateseq |
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Returns : string |
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Args : [optional] string |
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=cut |
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sub hmmstate_string{ |
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my ($self,$val) = @_; |
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if( defined $val ) { |
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$self->{'_hmmstate_string'} = $val; |
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} |
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return $self->{'_hmmstate_string'}; |
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} |
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=head2 homology_string |
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Title : homolgy_string |
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Usage : my $homology_str = $hsp->homology_string(); |
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Function: Homology string must be calculated for a WABA HSP so we can do |
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so here and cache the result so it is only done once |
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Returns : string |
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Args : none |
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=cut |
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sub homology_string{ |
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my ($self) = @_; |
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return ''; |
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} |
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1; |