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# BioPerl module for Bio::Restriction::IO |
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# |
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# Please direct questions and support issues to |
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# |
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# Cared for by Rob Edwards |
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# |
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# Copyright Rob Edwards |
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# |
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# You may distribute this module under the same terms as perl itself |
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# |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::Restriction::IO - Handler for sequence variation IO Formats |
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=head1 SYNOPSIS |
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use Bio::Restriction::IO; |
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$in = Bio::Restriction::IO->new(-file => "inputfilename" , |
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-format => 'withrefm'); |
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my $res = $in->read; # a Bio::Restriction::EnzymeCollection |
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=head1 DESCRIPTION |
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L is a handler module for the formats in the |
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Restriction IO set, e.g. C. It is the |
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officially sanctioned way of getting at the format objects, which most |
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people should use. |
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The structure, conventions and most of the code is inherited from |
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L. The main difference is that instead of using methods |
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C, you drop C<_seq> from the method name. |
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Also, instead of dealing only with individual L |
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objects, C will slurp in all enzymes into a |
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L object. |
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For more details, see documentation in L. |
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=head1 TO DO |
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At the moment, these can be use mainly to get a custom set if enzymes in |
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C or C formats into L or |
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L objects. Using C format is |
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highly experimental and is not recommmended at this time. |
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This class inherits from L for convenience sake, though this should |
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inherit from L. Get rid of L inheritance by |
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copying relevant methods in. |
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C methods are currently not implemented for any format except C. |
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Using C even with C format is not recommended as it does not |
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support multicut/multisite enzyme output. |
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Should additional formats be supported (such as XML)? |
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=head1 SEE ALSO |
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L, |
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L, |
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L |
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=head1 FEEDBACK |
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=head2 Mailing Lists |
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User feedback is an integral part of the evolution of this and other |
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Bioperl modules. Send your comments and suggestions preferably to the |
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Bioperl mailing lists Your participation is much appreciated. |
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bioperl-l@bioperl.org - General discussion |
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http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
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=head2 Support |
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Please direct usage questions or support issues to the mailing list: |
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I |
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rather than to the module maintainer directly. Many experienced and |
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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=head2 Reporting Bugs |
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report bugs to the Bioperl bug tracking system to help us keep track |
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the bugs and their resolution. Bug reports can be submitted via the |
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web: |
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https://github.com/bioperl/bioperl-live/issues |
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=head1 AUTHOR |
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Rob Edwards, redwards@utmem.edu |
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=head1 CONTRIBUTORS |
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Heikki Lehvaslaiho, heikki-at-bioperl-dot-org |
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=head1 APPENDIX |
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The rest of the documentation details each of the object |
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methods. Internal methods are usually preceded with a _ |
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=cut |
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# Let the code begin... |
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package Bio::Restriction::IO; |
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use strict; |
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use vars qw(%FORMAT); |
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use base qw(Bio::SeqIO); |
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%FORMAT = ( |
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'itype2' => 'itype2', |
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'8' => 'itype2', |
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'withrefm' => 'withrefm', |
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'31' => 'withrefm', |
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'base' => 'base', |
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'0' => 'base', |
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'bairoch' => 'bairoch', |
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'19' => 'bairoch', |
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'macvector' => 'bairoch', |
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'vectorNTI' => 'bairoch', |
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'neo' => 'prototype', |
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'prototype' => 'prototype' |
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); |
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=head2 new |
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Title : new |
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Usage : $stream = Bio::Restriction::IO->new(-file => $filename, |
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-format => 'Format') |
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Function: Returns a new seqstream |
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Returns : A Bio::Restriction::IO::Handler initialised with |
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the appropriate format |
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Args : -file => $filename |
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-format => format |
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-fh => filehandle to attach to |
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=cut |
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sub new { |
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my ($class, %param) = @_; |
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my ($format); |
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@param{ map { lc $_ } keys %param } = values %param; # lowercase keys |
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$format = $FORMAT{$param{'-format'}} if defined $param{'-format'}; |
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$format ||= $class->_guess_format( $param{-file} || $ARGV[0] ) |
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|| 'base'; |
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$format = "\L$format"; # normalize capitalization to lower case |
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return unless $class->_load_format_module($format); |
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return "Bio::Restriction::IO::$format"->new(%param); |
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} |
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=head2 format |
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Title : format |
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Usage : $format = $stream->format() |
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Function: Get the restriction format |
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Returns : restriction format |
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Args : none |
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=cut |
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# format() method inherited from Bio::Root::IO |
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sub _load_format_module { |
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my ($class, $format) = @_; |
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my $module = "Bio::Restriction::IO::" . $format; |
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my $ok; |
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eval { |
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$ok = $class->_load_module($module); |
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}; |
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if ( $@ ) { |
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print STDERR <
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$class: $format cannot be found |
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Exception $@ |
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For more information about the IO system please see the IO docs. |
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This includes ways of checking for formats at compile time, not run time |
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END |
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; |
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} |
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return $ok; |
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} |
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=head2 read |
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Title : read |
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Usage : $renzs = $stream->read |
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Function: reads all the restrction enzymes from the stream |
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Returns : a Bio::Restriction::EnzymeCollection object |
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Args : |
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203
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=cut |
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205
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sub read { |
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0
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0
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1
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my ($self, $seq) = @_; |
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$self->throw_not_implemented(); |
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} |
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sub next { |
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my ($self, $seq) = @_; |
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$self->throw_not_implemented(); |
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} |
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215
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sub next_seq { |
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1
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my ($self, $seq) = @_; |
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$self->throw_not_implemented(); |
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} |
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220
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=head2 write |
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222
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Title : write |
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Usage : $stream->write($seq) |
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Function: writes the $seq object into the stream |
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Returns : 1 for success and 0 for error |
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Args : Bio::Restriction::EnzymeCollection object |
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228
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=cut |
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230
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sub write { |
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0
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0
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1
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0
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my ($self, $seq) = @_; |
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0
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0
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$self->throw("Sorry, you cannot write to a generic ". |
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"Bio::Restricion::IO object."); |
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} |
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|
236
|
|
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|
|
|
|
sub write_seq { |
237
|
0
|
|
|
0
|
1
|
0
|
my ($self, $seq) = @_; |
238
|
0
|
|
|
|
|
0
|
$self->warn("These are not sequence objects. ". |
239
|
|
|
|
|
|
|
"Use method 'write' instead of 'write_seq'."); |
240
|
0
|
|
|
|
|
0
|
$self->write($seq); |
241
|
|
|
|
|
|
|
} |
242
|
|
|
|
|
|
|
|
243
|
|
|
|
|
|
|
=head2 _guess_format |
244
|
|
|
|
|
|
|
|
245
|
|
|
|
|
|
|
Title : _guess_format |
246
|
|
|
|
|
|
|
Usage : $obj->_guess_format($filename) |
247
|
|
|
|
|
|
|
Function: |
248
|
|
|
|
|
|
|
Example : |
249
|
|
|
|
|
|
|
Returns : guessed format of filename (lower case) |
250
|
|
|
|
|
|
|
Args : |
251
|
|
|
|
|
|
|
|
252
|
|
|
|
|
|
|
=cut |
253
|
|
|
|
|
|
|
|
254
|
|
|
|
|
|
|
sub _guess_format { |
255
|
5
|
|
|
5
|
|
7
|
my $class = shift; |
256
|
5
|
50
|
|
|
|
36
|
return unless $_ = shift; |
257
|
0
|
0
|
|
|
|
|
return 'flat' if /\.dat$/i; |
258
|
0
|
0
|
|
|
|
|
return 'xml' if /\.xml$/i; |
259
|
|
|
|
|
|
|
} |
260
|
|
|
|
|
|
|
|
261
|
|
|
|
|
|
|
|
262
|
|
|
|
|
|
|
1; |