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| 1 |  |  |  |  |  |  | # | 
| 2 |  |  |  |  |  |  | # BioPerl module for Bio::Restriction::IO | 
| 3 |  |  |  |  |  |  | # | 
| 4 |  |  |  |  |  |  | # Please direct questions and support issues to | 
| 5 |  |  |  |  |  |  | # | 
| 6 |  |  |  |  |  |  | # Cared for by Rob Edwards | 
| 7 |  |  |  |  |  |  | # | 
| 8 |  |  |  |  |  |  | # Copyright Rob Edwards | 
| 9 |  |  |  |  |  |  | # | 
| 10 |  |  |  |  |  |  | # You may distribute this module under the same terms as perl itself | 
| 11 |  |  |  |  |  |  | # | 
| 12 |  |  |  |  |  |  | # POD documentation - main docs before the code | 
| 13 |  |  |  |  |  |  |  | 
| 14 |  |  |  |  |  |  | =head1 NAME | 
| 15 |  |  |  |  |  |  |  | 
| 16 |  |  |  |  |  |  | Bio::Restriction::IO - Handler for sequence variation IO Formats | 
| 17 |  |  |  |  |  |  |  | 
| 18 |  |  |  |  |  |  | =head1 SYNOPSIS | 
| 19 |  |  |  |  |  |  |  | 
| 20 |  |  |  |  |  |  | use Bio::Restriction::IO; | 
| 21 |  |  |  |  |  |  |  | 
| 22 |  |  |  |  |  |  | $in  = Bio::Restriction::IO->new(-file => "inputfilename" , | 
| 23 |  |  |  |  |  |  | -format => 'withrefm'); | 
| 24 |  |  |  |  |  |  | my $res = $in->read; # a Bio::Restriction::EnzymeCollection | 
| 25 |  |  |  |  |  |  |  | 
| 26 |  |  |  |  |  |  | =head1 DESCRIPTION | 
| 27 |  |  |  |  |  |  |  | 
| 28 |  |  |  |  |  |  | L is a handler module for the formats in the | 
| 29 |  |  |  |  |  |  | Restriction IO set, e.g. C. It is the | 
| 30 |  |  |  |  |  |  | officially sanctioned way of getting at the format objects, which most | 
| 31 |  |  |  |  |  |  | people should use. | 
| 32 |  |  |  |  |  |  |  | 
| 33 |  |  |  |  |  |  | The structure, conventions and most of the code is inherited from | 
| 34 |  |  |  |  |  |  | L. The main difference is that instead of using methods | 
| 35 |  |  |  |  |  |  | C, you drop C<_seq> from the method name. | 
| 36 |  |  |  |  |  |  |  | 
| 37 |  |  |  |  |  |  | Also, instead of dealing only with individual L | 
| 38 |  |  |  |  |  |  | objects, C will slurp in all enzymes into a | 
| 39 |  |  |  |  |  |  | L object. | 
| 40 |  |  |  |  |  |  |  | 
| 41 |  |  |  |  |  |  | For more details, see documentation in L. | 
| 42 |  |  |  |  |  |  |  | 
| 43 |  |  |  |  |  |  | =head1 TO DO | 
| 44 |  |  |  |  |  |  |  | 
| 45 |  |  |  |  |  |  | At the moment, these can be use mainly to get a custom set if enzymes in | 
| 46 |  |  |  |  |  |  | C or C formats into L or | 
| 47 |  |  |  |  |  |  | L objects.  Using C format is | 
| 48 |  |  |  |  |  |  | highly experimental and is not recommmended at this time. | 
| 49 |  |  |  |  |  |  |  | 
| 50 |  |  |  |  |  |  | This class inherits from L for convenience sake, though this should | 
| 51 |  |  |  |  |  |  | inherit from L.  Get rid of L inheritance by | 
| 52 |  |  |  |  |  |  | copying relevant methods in. | 
| 53 |  |  |  |  |  |  |  | 
| 54 |  |  |  |  |  |  | C methods are currently not implemented for any format except C. | 
| 55 |  |  |  |  |  |  | Using C even with C format is not recommended as it does not | 
| 56 |  |  |  |  |  |  | support multicut/multisite enzyme output. | 
| 57 |  |  |  |  |  |  |  | 
| 58 |  |  |  |  |  |  | Should additional formats be supported (such as XML)? | 
| 59 |  |  |  |  |  |  |  | 
| 60 |  |  |  |  |  |  | =head1 SEE ALSO | 
| 61 |  |  |  |  |  |  |  | 
| 62 |  |  |  |  |  |  | L, | 
| 63 |  |  |  |  |  |  | L, | 
| 64 |  |  |  |  |  |  | L | 
| 65 |  |  |  |  |  |  |  | 
| 66 |  |  |  |  |  |  | =head1 FEEDBACK | 
| 67 |  |  |  |  |  |  |  | 
| 68 |  |  |  |  |  |  | =head2 Mailing Lists | 
| 69 |  |  |  |  |  |  |  | 
| 70 |  |  |  |  |  |  | User feedback is an integral part of the evolution of this and other | 
| 71 |  |  |  |  |  |  | Bioperl modules. Send your comments and suggestions preferably to the | 
| 72 |  |  |  |  |  |  | Bioperl mailing lists Your participation is much appreciated. | 
| 73 |  |  |  |  |  |  |  | 
| 74 |  |  |  |  |  |  | bioperl-l@bioperl.org                  - General discussion | 
| 75 |  |  |  |  |  |  | http://bioperl.org/wiki/Mailing_lists  - About the mailing lists | 
| 76 |  |  |  |  |  |  |  | 
| 77 |  |  |  |  |  |  | =head2 Support | 
| 78 |  |  |  |  |  |  |  | 
| 79 |  |  |  |  |  |  | Please direct usage questions or support issues to the mailing list: | 
| 80 |  |  |  |  |  |  |  | 
| 81 |  |  |  |  |  |  | I | 
| 82 |  |  |  |  |  |  |  | 
| 83 |  |  |  |  |  |  | rather than to the module maintainer directly. Many experienced and | 
| 84 |  |  |  |  |  |  | reponsive experts will be able look at the problem and quickly | 
| 85 |  |  |  |  |  |  | address it. Please include a thorough description of the problem | 
| 86 |  |  |  |  |  |  | with code and data examples if at all possible. | 
| 87 |  |  |  |  |  |  |  | 
| 88 |  |  |  |  |  |  | =head2 Reporting Bugs | 
| 89 |  |  |  |  |  |  |  | 
| 90 |  |  |  |  |  |  | report bugs to the Bioperl bug tracking system to help us keep track | 
| 91 |  |  |  |  |  |  | the bugs and their resolution.  Bug reports can be submitted via the | 
| 92 |  |  |  |  |  |  | web: | 
| 93 |  |  |  |  |  |  |  | 
| 94 |  |  |  |  |  |  | https://github.com/bioperl/bioperl-live/issues | 
| 95 |  |  |  |  |  |  |  | 
| 96 |  |  |  |  |  |  | =head1 AUTHOR | 
| 97 |  |  |  |  |  |  |  | 
| 98 |  |  |  |  |  |  | Rob Edwards, redwards@utmem.edu | 
| 99 |  |  |  |  |  |  |  | 
| 100 |  |  |  |  |  |  | =head1 CONTRIBUTORS | 
| 101 |  |  |  |  |  |  |  | 
| 102 |  |  |  |  |  |  | Heikki Lehvaslaiho, heikki-at-bioperl-dot-org | 
| 103 |  |  |  |  |  |  |  | 
| 104 |  |  |  |  |  |  | =head1 APPENDIX | 
| 105 |  |  |  |  |  |  |  | 
| 106 |  |  |  |  |  |  | The rest of the documentation details each of the object | 
| 107 |  |  |  |  |  |  | methods. Internal methods are usually preceded with a _ | 
| 108 |  |  |  |  |  |  |  | 
| 109 |  |  |  |  |  |  | =cut | 
| 110 |  |  |  |  |  |  |  | 
| 111 |  |  |  |  |  |  | # Let the code begin... | 
| 112 |  |  |  |  |  |  |  | 
| 113 |  |  |  |  |  |  | package Bio::Restriction::IO; | 
| 114 |  |  |  |  |  |  |  | 
| 115 | 4 |  |  | 4 |  | 906 | use strict; | 
|  | 4 |  |  |  |  | 5 |  | 
|  | 4 |  |  |  |  | 98 |  | 
| 116 | 4 |  |  | 4 |  | 13 | use vars qw(%FORMAT); | 
|  | 4 |  |  |  |  | 4 |  | 
|  | 4 |  |  |  |  | 128 |  | 
| 117 | 4 |  |  | 4 |  | 12 | use base qw(Bio::SeqIO); | 
|  | 4 |  |  |  |  | 3 |  | 
|  | 4 |  |  |  |  | 1402 |  | 
| 118 |  |  |  |  |  |  |  | 
| 119 |  |  |  |  |  |  | %FORMAT = ( | 
| 120 |  |  |  |  |  |  | 'itype2'    => 'itype2', | 
| 121 |  |  |  |  |  |  | '8'         => 'itype2', | 
| 122 |  |  |  |  |  |  | 'withrefm'  => 'withrefm', | 
| 123 |  |  |  |  |  |  | '31'        => 'withrefm', | 
| 124 |  |  |  |  |  |  | 'base'      => 'base', | 
| 125 |  |  |  |  |  |  | '0'         => 'base', | 
| 126 |  |  |  |  |  |  | 'bairoch'   => 'bairoch', | 
| 127 |  |  |  |  |  |  | '19'        => 'bairoch', | 
| 128 |  |  |  |  |  |  | 'macvector' => 'bairoch', | 
| 129 |  |  |  |  |  |  | 'vectorNTI' => 'bairoch', | 
| 130 |  |  |  |  |  |  | 'neo'       => 'prototype', | 
| 131 |  |  |  |  |  |  | 'prototype' => 'prototype' | 
| 132 |  |  |  |  |  |  | ); | 
| 133 |  |  |  |  |  |  |  | 
| 134 |  |  |  |  |  |  | =head2 new | 
| 135 |  |  |  |  |  |  |  | 
| 136 |  |  |  |  |  |  | Title   : new | 
| 137 |  |  |  |  |  |  | Usage   : $stream = Bio::Restriction::IO->new(-file => $filename, | 
| 138 |  |  |  |  |  |  | -format => 'Format') | 
| 139 |  |  |  |  |  |  | Function: Returns a new seqstream | 
| 140 |  |  |  |  |  |  | Returns : A Bio::Restriction::IO::Handler initialised with | 
| 141 |  |  |  |  |  |  | the appropriate format | 
| 142 |  |  |  |  |  |  | Args    : -file => $filename | 
| 143 |  |  |  |  |  |  | -format => format | 
| 144 |  |  |  |  |  |  | -fh => filehandle to attach to | 
| 145 |  |  |  |  |  |  |  | 
| 146 |  |  |  |  |  |  | =cut | 
| 147 |  |  |  |  |  |  |  | 
| 148 |  |  |  |  |  |  | sub new { | 
| 149 | 11 |  |  | 11 | 1 | 183 | my ($class, %param) = @_; | 
| 150 | 11 |  |  |  |  | 15 | my ($format); | 
| 151 |  |  |  |  |  |  |  | 
| 152 | 11 |  |  |  |  | 42 | @param{ map { lc $_ } keys %param } = values %param;  # lowercase keys | 
|  | 17 |  |  |  |  | 39 |  | 
| 153 | 11 | 100 |  |  |  | 46 | $format = $FORMAT{$param{'-format'}} if defined $param{'-format'}; | 
| 154 | 11 |  | 50 |  |  | 58 | $format ||= $class->_guess_format( $param{-file} || $ARGV[0] ) | 
|  |  |  | 66 |  |  |  |  | 
| 155 |  |  |  |  |  |  | || 'base'; | 
| 156 | 11 |  |  |  |  | 18 | $format = "\L$format"; # normalize capitalization to lower case | 
| 157 |  |  |  |  |  |  |  | 
| 158 | 11 | 50 |  |  |  | 33 | return unless $class->_load_format_module($format); | 
| 159 | 11 |  |  |  |  | 131 | return "Bio::Restriction::IO::$format"->new(%param); | 
| 160 |  |  |  |  |  |  | } | 
| 161 |  |  |  |  |  |  |  | 
| 162 |  |  |  |  |  |  |  | 
| 163 |  |  |  |  |  |  | =head2 format | 
| 164 |  |  |  |  |  |  |  | 
| 165 |  |  |  |  |  |  | Title   : format | 
| 166 |  |  |  |  |  |  | Usage   : $format = $stream->format() | 
| 167 |  |  |  |  |  |  | Function: Get the restriction format | 
| 168 |  |  |  |  |  |  | Returns : restriction format | 
| 169 |  |  |  |  |  |  | Args    : none | 
| 170 |  |  |  |  |  |  |  | 
| 171 |  |  |  |  |  |  | =cut | 
| 172 |  |  |  |  |  |  |  | 
| 173 |  |  |  |  |  |  | # format() method inherited from Bio::Root::IO | 
| 174 |  |  |  |  |  |  |  | 
| 175 |  |  |  |  |  |  |  | 
| 176 |  |  |  |  |  |  | sub _load_format_module { | 
| 177 | 11 |  |  | 11 |  | 18 | my ($class, $format) = @_; | 
| 178 | 11 |  |  |  |  | 25 | my $module = "Bio::Restriction::IO::" . $format; | 
| 179 | 11 |  |  |  |  | 11 | my $ok; | 
| 180 | 11 |  |  |  |  | 21 | eval { | 
| 181 | 11 |  |  |  |  | 64 | $ok = $class->_load_module($module); | 
| 182 |  |  |  |  |  |  | }; | 
| 183 | 11 | 50 |  |  |  | 27 | if ( $@ ) { | 
| 184 | 0 |  |  |  |  | 0 | print STDERR < | 
| 185 |  |  |  |  |  |  | $class: $format cannot be found | 
| 186 |  |  |  |  |  |  | Exception $@ | 
| 187 |  |  |  |  |  |  | For more information about the IO system please see the IO docs. | 
| 188 |  |  |  |  |  |  | This includes ways of checking for formats at compile time, not run time | 
| 189 |  |  |  |  |  |  | END | 
| 190 |  |  |  |  |  |  | ; | 
| 191 |  |  |  |  |  |  | } | 
| 192 | 11 |  |  |  |  | 31 | return $ok; | 
| 193 |  |  |  |  |  |  | } | 
| 194 |  |  |  |  |  |  |  | 
| 195 |  |  |  |  |  |  | =head2 read | 
| 196 |  |  |  |  |  |  |  | 
| 197 |  |  |  |  |  |  | Title   : read | 
| 198 |  |  |  |  |  |  | Usage   : $renzs = $stream->read | 
| 199 |  |  |  |  |  |  | Function: reads all the restrction enzymes from the stream | 
| 200 |  |  |  |  |  |  | Returns : a Bio::Restriction::EnzymeCollection object | 
| 201 |  |  |  |  |  |  | Args    : | 
| 202 |  |  |  |  |  |  |  | 
| 203 |  |  |  |  |  |  | =cut | 
| 204 |  |  |  |  |  |  |  | 
| 205 |  |  |  |  |  |  | sub read { | 
| 206 | 0 |  |  | 0 | 1 | 0 | my ($self, $seq) = @_; | 
| 207 | 0 |  |  |  |  | 0 | $self->throw_not_implemented(); | 
| 208 |  |  |  |  |  |  | } | 
| 209 |  |  |  |  |  |  |  | 
| 210 |  |  |  |  |  |  | sub next { | 
| 211 | 0 |  |  | 0 | 0 | 0 | my ($self, $seq) = @_; | 
| 212 | 0 |  |  |  |  | 0 | $self->throw_not_implemented(); | 
| 213 |  |  |  |  |  |  | } | 
| 214 |  |  |  |  |  |  |  | 
| 215 |  |  |  |  |  |  | sub next_seq { | 
| 216 | 0 |  |  | 0 | 1 | 0 | my ($self, $seq) = @_; | 
| 217 | 0 |  |  |  |  | 0 | $self->throw_not_implemented(); | 
| 218 |  |  |  |  |  |  | } | 
| 219 |  |  |  |  |  |  |  | 
| 220 |  |  |  |  |  |  | =head2 write | 
| 221 |  |  |  |  |  |  |  | 
| 222 |  |  |  |  |  |  | Title   : write | 
| 223 |  |  |  |  |  |  | Usage   : $stream->write($seq) | 
| 224 |  |  |  |  |  |  | Function: writes the $seq object into the stream | 
| 225 |  |  |  |  |  |  | Returns : 1 for success and 0 for error | 
| 226 |  |  |  |  |  |  | Args    : Bio::Restriction::EnzymeCollection object | 
| 227 |  |  |  |  |  |  |  | 
| 228 |  |  |  |  |  |  | =cut | 
| 229 |  |  |  |  |  |  |  | 
| 230 |  |  |  |  |  |  | sub write { | 
| 231 | 0 |  |  | 0 | 1 | 0 | my ($self, $seq) = @_; | 
| 232 | 0 |  |  |  |  | 0 | $self->throw("Sorry, you cannot write to a generic ". | 
| 233 |  |  |  |  |  |  | "Bio::Restricion::IO object."); | 
| 234 |  |  |  |  |  |  | } | 
| 235 |  |  |  |  |  |  |  | 
| 236 |  |  |  |  |  |  | sub write_seq { | 
| 237 | 0 |  |  | 0 | 1 | 0 | my ($self, $seq) = @_; | 
| 238 | 0 |  |  |  |  | 0 | $self->warn("These are not sequence objects. ". | 
| 239 |  |  |  |  |  |  | "Use method 'write' instead of 'write_seq'."); | 
| 240 | 0 |  |  |  |  | 0 | $self->write($seq); | 
| 241 |  |  |  |  |  |  | } | 
| 242 |  |  |  |  |  |  |  | 
| 243 |  |  |  |  |  |  | =head2 _guess_format | 
| 244 |  |  |  |  |  |  |  | 
| 245 |  |  |  |  |  |  | Title   : _guess_format | 
| 246 |  |  |  |  |  |  | Usage   : $obj->_guess_format($filename) | 
| 247 |  |  |  |  |  |  | Function: | 
| 248 |  |  |  |  |  |  | Example : | 
| 249 |  |  |  |  |  |  | Returns : guessed format of filename (lower case) | 
| 250 |  |  |  |  |  |  | Args    : | 
| 251 |  |  |  |  |  |  |  | 
| 252 |  |  |  |  |  |  | =cut | 
| 253 |  |  |  |  |  |  |  | 
| 254 |  |  |  |  |  |  | sub _guess_format { | 
| 255 | 5 |  |  | 5 |  | 7 | my $class = shift; | 
| 256 | 5 | 50 |  |  |  | 36 | return  unless $_ = shift; | 
| 257 | 0 | 0 |  |  |  |  | return 'flat' if /\.dat$/i; | 
| 258 | 0 | 0 |  |  |  |  | return 'xml'  if /\.xml$/i; | 
| 259 |  |  |  |  |  |  | } | 
| 260 |  |  |  |  |  |  |  | 
| 261 |  |  |  |  |  |  |  | 
| 262 |  |  |  |  |  |  | 1; |