| line |
stmt |
bran |
cond |
sub |
pod |
time |
code |
|
1
|
|
|
|
|
|
|
# |
|
2
|
|
|
|
|
|
|
# BioPerl module for Bio::Map::PositionI |
|
3
|
|
|
|
|
|
|
# |
|
4
|
|
|
|
|
|
|
# Please direct questions and support issues to |
|
5
|
|
|
|
|
|
|
# |
|
6
|
|
|
|
|
|
|
# Cared for by Sendu Bala |
|
7
|
|
|
|
|
|
|
# |
|
8
|
|
|
|
|
|
|
# Copyright Jason Stajich |
|
9
|
|
|
|
|
|
|
# |
|
10
|
|
|
|
|
|
|
# You may distribute this module under the same terms as perl itself |
|
11
|
|
|
|
|
|
|
|
|
12
|
|
|
|
|
|
|
# POD documentation - main docs before the code |
|
13
|
|
|
|
|
|
|
|
|
14
|
|
|
|
|
|
|
=head1 NAME |
|
15
|
|
|
|
|
|
|
|
|
16
|
|
|
|
|
|
|
Bio::Map::PositionI - Abstracts the notion of a position having a value in the context of a marker and a Map |
|
17
|
|
|
|
|
|
|
|
|
18
|
|
|
|
|
|
|
=head1 SYNOPSIS |
|
19
|
|
|
|
|
|
|
|
|
20
|
|
|
|
|
|
|
# do not use this module directly |
|
21
|
|
|
|
|
|
|
# See Bio::Map::Position for an example of |
|
22
|
|
|
|
|
|
|
# implementation. |
|
23
|
|
|
|
|
|
|
|
|
24
|
|
|
|
|
|
|
=head1 DESCRIPTION |
|
25
|
|
|
|
|
|
|
|
|
26
|
|
|
|
|
|
|
This object stores one of the postions that a mappable object |
|
27
|
|
|
|
|
|
|
(e.g. Marker) may have in a map. |
|
28
|
|
|
|
|
|
|
|
|
29
|
|
|
|
|
|
|
Positions can have non-numeric values or other methods to store the locations, |
|
30
|
|
|
|
|
|
|
so they have a method numeric() which does the conversion. numeric() |
|
31
|
|
|
|
|
|
|
returns the position in a form that can be compared between other positions of |
|
32
|
|
|
|
|
|
|
the same type. It is not necessarily a value suitable for sorting positions (it |
|
33
|
|
|
|
|
|
|
may be the distance from the previous position); for that purpose the result of |
|
34
|
|
|
|
|
|
|
sortable() should be used. |
|
35
|
|
|
|
|
|
|
|
|
36
|
|
|
|
|
|
|
A 'position', in addition to being a single point, can also be an area and so |
|
37
|
|
|
|
|
|
|
can be imagined as a range and compared with other positions on the basis of |
|
38
|
|
|
|
|
|
|
overlap, intersection etc. |
|
39
|
|
|
|
|
|
|
|
|
40
|
|
|
|
|
|
|
=head1 FEEDBACK |
|
41
|
|
|
|
|
|
|
|
|
42
|
|
|
|
|
|
|
=head2 Mailing Lists |
|
43
|
|
|
|
|
|
|
|
|
44
|
|
|
|
|
|
|
User feedback is an integral part of the evolution of this and other |
|
45
|
|
|
|
|
|
|
Bioperl modules. Send your comments and suggestions preferably to |
|
46
|
|
|
|
|
|
|
the Bioperl mailing list. Your participation is much appreciated. |
|
47
|
|
|
|
|
|
|
|
|
48
|
|
|
|
|
|
|
bioperl-l@bioperl.org - General discussion |
|
49
|
|
|
|
|
|
|
http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
|
50
|
|
|
|
|
|
|
|
|
51
|
|
|
|
|
|
|
=head2 Support |
|
52
|
|
|
|
|
|
|
|
|
53
|
|
|
|
|
|
|
Please direct usage questions or support issues to the mailing list: |
|
54
|
|
|
|
|
|
|
|
|
55
|
|
|
|
|
|
|
I |
|
56
|
|
|
|
|
|
|
|
|
57
|
|
|
|
|
|
|
rather than to the module maintainer directly. Many experienced and |
|
58
|
|
|
|
|
|
|
reponsive experts will be able look at the problem and quickly |
|
59
|
|
|
|
|
|
|
address it. Please include a thorough description of the problem |
|
60
|
|
|
|
|
|
|
with code and data examples if at all possible. |
|
61
|
|
|
|
|
|
|
|
|
62
|
|
|
|
|
|
|
=head2 Reporting Bugs |
|
63
|
|
|
|
|
|
|
|
|
64
|
|
|
|
|
|
|
Report bugs to the Bioperl bug tracking system to help us keep track |
|
65
|
|
|
|
|
|
|
of the bugs and their resolution. Bug reports can be submitted via the |
|
66
|
|
|
|
|
|
|
web: |
|
67
|
|
|
|
|
|
|
|
|
68
|
|
|
|
|
|
|
https://github.com/bioperl/bioperl-live/issues |
|
69
|
|
|
|
|
|
|
|
|
70
|
|
|
|
|
|
|
=head1 AUTHOR - Jason Stajich |
|
71
|
|
|
|
|
|
|
|
|
72
|
|
|
|
|
|
|
Email jason-at-bioperl.org |
|
73
|
|
|
|
|
|
|
|
|
74
|
|
|
|
|
|
|
=head1 CONTRIBUTORS |
|
75
|
|
|
|
|
|
|
|
|
76
|
|
|
|
|
|
|
Lincoln Stein, lstein-at-cshl.org |
|
77
|
|
|
|
|
|
|
Heikki Lehvaslaiho, heikki-at-bioperl-dot-org |
|
78
|
|
|
|
|
|
|
Sendu Bala, bix@sendu.me.uk |
|
79
|
|
|
|
|
|
|
|
|
80
|
|
|
|
|
|
|
=head1 APPENDIX |
|
81
|
|
|
|
|
|
|
|
|
82
|
|
|
|
|
|
|
The rest of the documentation details each of the object methods. |
|
83
|
|
|
|
|
|
|
Internal methods are usually preceded with a _ |
|
84
|
|
|
|
|
|
|
|
|
85
|
|
|
|
|
|
|
=cut |
|
86
|
|
|
|
|
|
|
|
|
87
|
|
|
|
|
|
|
# Let the code begin... |
|
88
|
|
|
|
|
|
|
|
|
89
|
|
|
|
|
|
|
package Bio::Map::PositionI; |
|
90
|
9
|
|
|
9
|
|
41
|
use strict; |
|
|
9
|
|
|
|
|
11
|
|
|
|
9
|
|
|
|
|
210
|
|
|
91
|
9
|
|
|
9
|
|
1441
|
use Bio::Map::PositionHandler; |
|
|
9
|
|
|
|
|
15
|
|
|
|
9
|
|
|
|
|
180
|
|
|
92
|
9
|
|
|
9
|
|
2941
|
use Bio::Map::Mappable; |
|
|
9
|
|
|
|
|
15
|
|
|
|
9
|
|
|
|
|
247
|
|
|
93
|
9
|
|
|
9
|
|
42
|
use Scalar::Util qw(looks_like_number); |
|
|
9
|
|
|
|
|
10
|
|
|
|
9
|
|
|
|
|
477
|
|
|
94
|
|
|
|
|
|
|
|
|
95
|
9
|
|
|
9
|
|
37
|
use base qw(Bio::Map::EntityI Bio::RangeI); |
|
|
9
|
|
|
|
|
11
|
|
|
|
9
|
|
|
|
|
9964
|
|
|
96
|
|
|
|
|
|
|
|
|
97
|
|
|
|
|
|
|
=head2 EntityI methods |
|
98
|
|
|
|
|
|
|
|
|
99
|
|
|
|
|
|
|
These are fundamental to coordination of Positions and other entities, so are |
|
100
|
|
|
|
|
|
|
implemented at the interface level |
|
101
|
|
|
|
|
|
|
|
|
102
|
|
|
|
|
|
|
=cut |
|
103
|
|
|
|
|
|
|
|
|
104
|
|
|
|
|
|
|
=head2 get_position_handler |
|
105
|
|
|
|
|
|
|
|
|
106
|
|
|
|
|
|
|
Title : get_position_handler |
|
107
|
|
|
|
|
|
|
Usage : my $position_handler = $entity->get_position_handler(); |
|
108
|
|
|
|
|
|
|
Function: Gets a PositionHandlerI that $entity is registered with. |
|
109
|
|
|
|
|
|
|
Returns : Bio::Map::PositionHandlerI object |
|
110
|
|
|
|
|
|
|
Args : none |
|
111
|
|
|
|
|
|
|
|
|
112
|
|
|
|
|
|
|
=cut |
|
113
|
|
|
|
|
|
|
|
|
114
|
|
|
|
|
|
|
sub get_position_handler { |
|
115
|
4059
|
|
|
4059
|
1
|
3051
|
my $self = shift; |
|
116
|
4059
|
100
|
|
|
|
5424
|
unless (defined $self->{_eh}) { |
|
117
|
190
|
|
|
|
|
541
|
my $ph = Bio::Map::PositionHandler->new(-self => $self); |
|
118
|
190
|
|
|
|
|
235
|
$self->{_eh} = $ph; |
|
119
|
190
|
|
|
|
|
300
|
$ph->register; |
|
120
|
|
|
|
|
|
|
} |
|
121
|
4059
|
|
|
|
|
7138
|
return $self->{_eh}; |
|
122
|
|
|
|
|
|
|
} |
|
123
|
|
|
|
|
|
|
|
|
124
|
|
|
|
|
|
|
=head2 PositionHandlerI-related methods |
|
125
|
|
|
|
|
|
|
|
|
126
|
|
|
|
|
|
|
These are fundamental to coordination of Positions and other entities, so are |
|
127
|
|
|
|
|
|
|
implemented at the interface level |
|
128
|
|
|
|
|
|
|
|
|
129
|
|
|
|
|
|
|
=cut |
|
130
|
|
|
|
|
|
|
|
|
131
|
|
|
|
|
|
|
=head2 map |
|
132
|
|
|
|
|
|
|
|
|
133
|
|
|
|
|
|
|
Title : map |
|
134
|
|
|
|
|
|
|
Usage : my $map = $position->map(); |
|
135
|
|
|
|
|
|
|
$position->map($map); |
|
136
|
|
|
|
|
|
|
Function: Get/Set the map the position is in. |
|
137
|
|
|
|
|
|
|
Returns : L |
|
138
|
|
|
|
|
|
|
Args : none to get |
|
139
|
|
|
|
|
|
|
new L to set |
|
140
|
|
|
|
|
|
|
|
|
141
|
|
|
|
|
|
|
=cut |
|
142
|
|
|
|
|
|
|
|
|
143
|
|
|
|
|
|
|
sub map { |
|
144
|
3550
|
|
|
3550
|
1
|
3106
|
my ($self, $map) = @_; |
|
145
|
3550
|
|
|
|
|
3989
|
return $self->get_position_handler->map($map); |
|
146
|
|
|
|
|
|
|
} |
|
147
|
|
|
|
|
|
|
|
|
148
|
|
|
|
|
|
|
=head2 element |
|
149
|
|
|
|
|
|
|
|
|
150
|
|
|
|
|
|
|
Title : element |
|
151
|
|
|
|
|
|
|
Usage : my $element = $position->element(); |
|
152
|
|
|
|
|
|
|
$position->element($element); |
|
153
|
|
|
|
|
|
|
Function: Get/Set the element the position is for. |
|
154
|
|
|
|
|
|
|
Returns : L |
|
155
|
|
|
|
|
|
|
Args : none to get |
|
156
|
|
|
|
|
|
|
new L to set |
|
157
|
|
|
|
|
|
|
|
|
158
|
|
|
|
|
|
|
=cut |
|
159
|
|
|
|
|
|
|
|
|
160
|
|
|
|
|
|
|
sub element { |
|
161
|
107
|
|
|
107
|
1
|
93
|
my ($self, $element) = @_; |
|
162
|
107
|
|
|
|
|
148
|
return $self->get_position_handler->element($element); |
|
163
|
|
|
|
|
|
|
} |
|
164
|
|
|
|
|
|
|
|
|
165
|
|
|
|
|
|
|
=head2 marker |
|
166
|
|
|
|
|
|
|
|
|
167
|
|
|
|
|
|
|
Title : marker |
|
168
|
|
|
|
|
|
|
Function: This is a synonym of the element() method |
|
169
|
|
|
|
|
|
|
Status : deprecated, will be removed in the next version |
|
170
|
|
|
|
|
|
|
|
|
171
|
|
|
|
|
|
|
=cut |
|
172
|
|
|
|
|
|
|
|
|
173
|
|
|
|
|
|
|
*marker = \&element; |
|
174
|
|
|
|
|
|
|
|
|
175
|
|
|
|
|
|
|
=head2 PositionI-specific methods |
|
176
|
|
|
|
|
|
|
|
|
177
|
|
|
|
|
|
|
=cut |
|
178
|
|
|
|
|
|
|
|
|
179
|
|
|
|
|
|
|
=head2 value |
|
180
|
|
|
|
|
|
|
|
|
181
|
|
|
|
|
|
|
Title : value |
|
182
|
|
|
|
|
|
|
Usage : my $pos = $position->value(); |
|
183
|
|
|
|
|
|
|
Function: Get/Set the value for this position |
|
184
|
|
|
|
|
|
|
Returns : scalar, value |
|
185
|
|
|
|
|
|
|
Args : [optional] new value to set |
|
186
|
|
|
|
|
|
|
|
|
187
|
|
|
|
|
|
|
=cut |
|
188
|
|
|
|
|
|
|
|
|
189
|
|
|
|
|
|
|
sub value { |
|
190
|
0
|
|
|
0
|
1
|
0
|
my $self = shift; |
|
191
|
0
|
|
|
|
|
0
|
$self->throw_not_implemented(); |
|
192
|
|
|
|
|
|
|
} |
|
193
|
|
|
|
|
|
|
|
|
194
|
|
|
|
|
|
|
=head2 numeric |
|
195
|
|
|
|
|
|
|
|
|
196
|
|
|
|
|
|
|
Title : numeric |
|
197
|
|
|
|
|
|
|
Usage : my $num = $position->numeric; |
|
198
|
|
|
|
|
|
|
Function: Read-only method that is guaranteed to return a numeric |
|
199
|
|
|
|
|
|
|
representation of the start of this position. |
|
200
|
|
|
|
|
|
|
Returns : scalar numeric |
|
201
|
|
|
|
|
|
|
Args : none to get the co-ordinate normally (see absolute() method), OR |
|
202
|
|
|
|
|
|
|
Bio::Map::RelativeI to get the co-ordinate converted to be |
|
203
|
|
|
|
|
|
|
relative to what this Relative describes. |
|
204
|
|
|
|
|
|
|
|
|
205
|
|
|
|
|
|
|
=cut |
|
206
|
|
|
|
|
|
|
|
|
207
|
|
|
|
|
|
|
sub numeric { |
|
208
|
0
|
|
|
0
|
1
|
0
|
my $self = shift; |
|
209
|
0
|
|
|
|
|
0
|
$self->throw_not_implemented(); |
|
210
|
|
|
|
|
|
|
} |
|
211
|
|
|
|
|
|
|
|
|
212
|
|
|
|
|
|
|
=head2 sortable |
|
213
|
|
|
|
|
|
|
|
|
214
|
|
|
|
|
|
|
Title : sortable |
|
215
|
|
|
|
|
|
|
Usage : my $num = $position->sortable(); |
|
216
|
|
|
|
|
|
|
Function: Read-only method that is guaranteed to return a value suitable |
|
217
|
|
|
|
|
|
|
for correctly sorting this kind of position amongst other positions |
|
218
|
|
|
|
|
|
|
of the same kind on the same map. Note that sorting different kinds |
|
219
|
|
|
|
|
|
|
of position together is unlikely to give sane results. |
|
220
|
|
|
|
|
|
|
Returns : numeric |
|
221
|
|
|
|
|
|
|
Args : none |
|
222
|
|
|
|
|
|
|
|
|
223
|
|
|
|
|
|
|
=cut |
|
224
|
|
|
|
|
|
|
|
|
225
|
|
|
|
|
|
|
sub sortable { |
|
226
|
0
|
|
|
0
|
1
|
0
|
my $self = shift; |
|
227
|
0
|
|
|
|
|
0
|
$self->throw_not_implemented(); |
|
228
|
|
|
|
|
|
|
} |
|
229
|
|
|
|
|
|
|
|
|
230
|
|
|
|
|
|
|
=head2 relative |
|
231
|
|
|
|
|
|
|
|
|
232
|
|
|
|
|
|
|
Title : relative |
|
233
|
|
|
|
|
|
|
Usage : my $relative = $position->relative(); |
|
234
|
|
|
|
|
|
|
$position->relative($relative); |
|
235
|
|
|
|
|
|
|
Function: Get/set the thing this Position's coordinates (numerical(), start(), |
|
236
|
|
|
|
|
|
|
end()) are relative to, as described by a Relative object. |
|
237
|
|
|
|
|
|
|
Returns : Bio::Map::RelativeI (default is one describing "relative to the |
|
238
|
|
|
|
|
|
|
start of the Position's map") |
|
239
|
|
|
|
|
|
|
Args : none to get, OR |
|
240
|
|
|
|
|
|
|
Bio::Map::RelativeI to set |
|
241
|
|
|
|
|
|
|
|
|
242
|
|
|
|
|
|
|
=cut |
|
243
|
|
|
|
|
|
|
|
|
244
|
|
|
|
|
|
|
sub relative { |
|
245
|
0
|
|
|
0
|
1
|
0
|
my $self = shift; |
|
246
|
0
|
|
|
|
|
0
|
$self->throw_not_implemented(); |
|
247
|
|
|
|
|
|
|
} |
|
248
|
|
|
|
|
|
|
|
|
249
|
|
|
|
|
|
|
=head2 absolute |
|
250
|
|
|
|
|
|
|
|
|
251
|
|
|
|
|
|
|
Title : absolute |
|
252
|
|
|
|
|
|
|
Usage : my $absolute = $position->absolute(); |
|
253
|
|
|
|
|
|
|
$position->absolute($absolute); |
|
254
|
|
|
|
|
|
|
Function: Get/set how this Position's co-ordinates (numerical(), start(), |
|
255
|
|
|
|
|
|
|
end()) are reported. When absolute is off, co-ordinates are |
|
256
|
|
|
|
|
|
|
relative to the thing described by relative(). Ie. the value |
|
257
|
|
|
|
|
|
|
returned by start() will be the same as the value you set start() |
|
258
|
|
|
|
|
|
|
to. When absolute is on, co-ordinates are converted to be relative |
|
259
|
|
|
|
|
|
|
to the start of the map. |
|
260
|
|
|
|
|
|
|
|
|
261
|
|
|
|
|
|
|
So if relative() currently points to a Relative object describing |
|
262
|
|
|
|
|
|
|
"relative to another position which is 100 bp from the start of |
|
263
|
|
|
|
|
|
|
the map", this Position's start() had been set to 50 and absolute() |
|
264
|
|
|
|
|
|
|
returns 1, $position->start() will return 150. If absolute() returns |
|
265
|
|
|
|
|
|
|
0 in the same situation, $position->start() would return 50. |
|
266
|
|
|
|
|
|
|
|
|
267
|
|
|
|
|
|
|
Returns : boolean (default 0) |
|
268
|
|
|
|
|
|
|
Args : none to get, OR |
|
269
|
|
|
|
|
|
|
boolean to set |
|
270
|
|
|
|
|
|
|
|
|
271
|
|
|
|
|
|
|
=cut |
|
272
|
|
|
|
|
|
|
|
|
273
|
|
|
|
|
|
|
sub absolute { |
|
274
|
0
|
|
|
0
|
1
|
0
|
my $self = shift; |
|
275
|
0
|
|
|
|
|
0
|
$self->throw_not_implemented(); |
|
276
|
|
|
|
|
|
|
} |
|
277
|
|
|
|
|
|
|
|
|
278
|
|
|
|
|
|
|
=head2 RangeI-based methods |
|
279
|
|
|
|
|
|
|
|
|
280
|
|
|
|
|
|
|
=cut |
|
281
|
|
|
|
|
|
|
|
|
282
|
|
|
|
|
|
|
=head2 start |
|
283
|
|
|
|
|
|
|
|
|
284
|
|
|
|
|
|
|
Title : start |
|
285
|
|
|
|
|
|
|
Usage : my $start = $position->start(); |
|
286
|
|
|
|
|
|
|
$position->start($start); |
|
287
|
|
|
|
|
|
|
Function: Get/set the start co-ordinate of this position. |
|
288
|
|
|
|
|
|
|
Returns : the start of this position |
|
289
|
|
|
|
|
|
|
Args : scalar numeric to set, OR |
|
290
|
|
|
|
|
|
|
none to get the co-ordinate normally (see absolute() method), OR |
|
291
|
|
|
|
|
|
|
Bio::Map::RelativeI to get the co-ordinate converted to be |
|
292
|
|
|
|
|
|
|
relative to what this Relative describes. |
|
293
|
|
|
|
|
|
|
|
|
294
|
|
|
|
|
|
|
=cut |
|
295
|
|
|
|
|
|
|
|
|
296
|
|
|
|
|
|
|
=head2 end |
|
297
|
|
|
|
|
|
|
|
|
298
|
|
|
|
|
|
|
Title : end |
|
299
|
|
|
|
|
|
|
Usage : my $end = $position->end(); |
|
300
|
|
|
|
|
|
|
$position->end($end); |
|
301
|
|
|
|
|
|
|
Function: Get/set the end co-ordinate of this position. |
|
302
|
|
|
|
|
|
|
Returns : the end of this position |
|
303
|
|
|
|
|
|
|
Args : scalar numeric to set, OR |
|
304
|
|
|
|
|
|
|
none to get the co-ordinate normally (see absolute() method), OR |
|
305
|
|
|
|
|
|
|
Bio::Map::RelativeI to get the co-ordinate converted to be |
|
306
|
|
|
|
|
|
|
relative to what this Relative describes. |
|
307
|
|
|
|
|
|
|
|
|
308
|
|
|
|
|
|
|
=cut |
|
309
|
|
|
|
|
|
|
|
|
310
|
|
|
|
|
|
|
=head2 length |
|
311
|
|
|
|
|
|
|
|
|
312
|
|
|
|
|
|
|
Title : length |
|
313
|
|
|
|
|
|
|
Usage : $length = $position->length(); |
|
314
|
|
|
|
|
|
|
Function: Get the length of this position. |
|
315
|
|
|
|
|
|
|
Returns : the length of this position |
|
316
|
|
|
|
|
|
|
Args : none |
|
317
|
|
|
|
|
|
|
|
|
318
|
|
|
|
|
|
|
=cut |
|
319
|
|
|
|
|
|
|
|
|
320
|
|
|
|
|
|
|
=head2 strand |
|
321
|
|
|
|
|
|
|
|
|
322
|
|
|
|
|
|
|
Title : strand |
|
323
|
|
|
|
|
|
|
Usage : $strand = $position->strand(); |
|
324
|
|
|
|
|
|
|
Function: Get the strand of this position; it is always 1 since maps to not |
|
325
|
|
|
|
|
|
|
have strands. |
|
326
|
|
|
|
|
|
|
Returns : 1 |
|
327
|
|
|
|
|
|
|
Args : none |
|
328
|
|
|
|
|
|
|
|
|
329
|
|
|
|
|
|
|
=cut |
|
330
|
|
|
|
|
|
|
|
|
331
|
|
|
|
|
|
|
sub strand { |
|
332
|
202
|
|
|
202
|
1
|
383
|
return 1; |
|
333
|
|
|
|
|
|
|
} |
|
334
|
|
|
|
|
|
|
|
|
335
|
|
|
|
|
|
|
=head2 toString |
|
336
|
|
|
|
|
|
|
|
|
337
|
|
|
|
|
|
|
Title : toString |
|
338
|
|
|
|
|
|
|
Usage : print $position->toString(), "\n"; |
|
339
|
|
|
|
|
|
|
Function: stringifies this range |
|
340
|
|
|
|
|
|
|
Returns : a string representation of the range of this Position |
|
341
|
|
|
|
|
|
|
Args : optional Bio::Map::RelativeI to have the co-ordinates reported |
|
342
|
|
|
|
|
|
|
relative to the thing described by that Relative |
|
343
|
|
|
|
|
|
|
|
|
344
|
|
|
|
|
|
|
=cut |
|
345
|
|
|
|
|
|
|
|
|
346
|
|
|
|
|
|
|
sub toString { |
|
347
|
0
|
|
|
0
|
1
|
0
|
my $self = shift; |
|
348
|
0
|
|
|
|
|
0
|
$self->throw_not_implemented(); |
|
349
|
|
|
|
|
|
|
} |
|
350
|
|
|
|
|
|
|
|
|
351
|
|
|
|
|
|
|
=head1 RangeI-related methods |
|
352
|
|
|
|
|
|
|
|
|
353
|
|
|
|
|
|
|
These methods work by considering only the values of start() and end(), as |
|
354
|
|
|
|
|
|
|
modified by considering every such co-ordinate relative to the start of the map |
|
355
|
|
|
|
|
|
|
(ie. absolute(1) is set temporarily during the calculation), or any supplied |
|
356
|
|
|
|
|
|
|
Relative. For the boolean methods, when the comparison Position is on the same |
|
357
|
|
|
|
|
|
|
map as the calling Position, there is no point supplying a Relative since the |
|
358
|
|
|
|
|
|
|
answer will be the same as without. Relative is most useful when comparing |
|
359
|
|
|
|
|
|
|
Positions on different maps and you have a Relative that describes some special |
|
360
|
|
|
|
|
|
|
place on each map like 'the start of the gene', where the actual start of the |
|
361
|
|
|
|
|
|
|
gene relative to the start of the map is different for each map. |
|
362
|
|
|
|
|
|
|
|
|
363
|
|
|
|
|
|
|
The methods do not consider maps during their calculations - things on different |
|
364
|
|
|
|
|
|
|
maps can overlap/contain/intersect/etc. each other. |
|
365
|
|
|
|
|
|
|
|
|
366
|
|
|
|
|
|
|
The geometrical methods (intersect, union etc.) do things to the geometry of |
|
367
|
|
|
|
|
|
|
ranges, and return Bio::Map::PositionI compliant objects or triplets (start, |
|
368
|
|
|
|
|
|
|
stop, strand) from which new positions could be built. When a PositionI is made |
|
369
|
|
|
|
|
|
|
it will have a map transferred to it if all the arguments shared the same map. |
|
370
|
|
|
|
|
|
|
If a Relative was supplied the result will have that same Relative. |
|
371
|
|
|
|
|
|
|
|
|
372
|
|
|
|
|
|
|
Note that the strand-testing args are there for compatibility with the RangeI |
|
373
|
|
|
|
|
|
|
interface. They have no meaning when only using PositionI objects since maps do |
|
374
|
|
|
|
|
|
|
not have strands. Typically you will just set the argument to undef if you want |
|
375
|
|
|
|
|
|
|
to supply the argument after it. |
|
376
|
|
|
|
|
|
|
|
|
377
|
|
|
|
|
|
|
=head2 equals |
|
378
|
|
|
|
|
|
|
|
|
379
|
|
|
|
|
|
|
Title : equals |
|
380
|
|
|
|
|
|
|
Usage : if ($p1->equals($p2)) {...} |
|
381
|
|
|
|
|
|
|
Function: Test whether $p1 has the same start, end, length as $p2. |
|
382
|
|
|
|
|
|
|
Returns : true if they are describing the same position (regardless of map) |
|
383
|
|
|
|
|
|
|
Args : arg #1 = a Bio::RangeI (eg. a Bio::Map::Position) to compare this |
|
384
|
|
|
|
|
|
|
one to (mandatory) |
|
385
|
|
|
|
|
|
|
arg #2 = optional strand-testing arg ('strong', 'weak', 'ignore') |
|
386
|
|
|
|
|
|
|
arg #3 = optional Bio::Map::RelativeI to ask if the Positions |
|
387
|
|
|
|
|
|
|
equal in terms of their relative position to the thing |
|
388
|
|
|
|
|
|
|
described by that Relative |
|
389
|
|
|
|
|
|
|
|
|
390
|
|
|
|
|
|
|
=cut |
|
391
|
|
|
|
|
|
|
|
|
392
|
|
|
|
|
|
|
sub equals { |
|
393
|
|
|
|
|
|
|
# overriding the RangeI implementation so we can handle Relative |
|
394
|
8
|
|
|
8
|
1
|
498
|
my ($self, $other, $so, $rel) = @_; |
|
395
|
|
|
|
|
|
|
|
|
396
|
8
|
|
|
|
|
29
|
my ($own_start, $own_end) = $self->_pre_rangei($self, $rel); |
|
397
|
8
|
|
|
|
|
17
|
my ($other_start, $other_end) = $self->_pre_rangei($other, $rel); |
|
398
|
|
|
|
|
|
|
|
|
399
|
8
|
|
66
|
|
|
30
|
return ($self->_testStrand($other, $so) and |
|
400
|
|
|
|
|
|
|
$own_start == $other_start and $own_end == $other_end); |
|
401
|
|
|
|
|
|
|
} |
|
402
|
|
|
|
|
|
|
|
|
403
|
|
|
|
|
|
|
|
|
404
|
|
|
|
|
|
|
=head2 less_than |
|
405
|
|
|
|
|
|
|
|
|
406
|
|
|
|
|
|
|
Title : less_than |
|
407
|
|
|
|
|
|
|
Usage : if ($position->less_than($other_position)) {...} |
|
408
|
|
|
|
|
|
|
Function: Ask if this Position ends before another starts. |
|
409
|
|
|
|
|
|
|
Returns : boolean |
|
410
|
|
|
|
|
|
|
Args : arg #1 = a Bio::RangeI (eg. a Bio::Map::Position) to compare this |
|
411
|
|
|
|
|
|
|
one to (mandatory) |
|
412
|
|
|
|
|
|
|
arg #2 = optional Bio::Map::RelativeI to ask if the Position is less |
|
413
|
|
|
|
|
|
|
in terms of their relative position to the thing described |
|
414
|
|
|
|
|
|
|
by that Relative |
|
415
|
|
|
|
|
|
|
|
|
416
|
|
|
|
|
|
|
=cut |
|
417
|
|
|
|
|
|
|
|
|
418
|
|
|
|
|
|
|
sub less_than { |
|
419
|
3
|
|
|
3
|
1
|
4
|
my ($self, $other, $rel) = @_; |
|
420
|
|
|
|
|
|
|
|
|
421
|
3
|
|
|
|
|
9
|
my ($own_start, $own_end) = $self->_pre_rangei($self, $rel); |
|
422
|
3
|
|
|
|
|
7
|
my ($other_start, $other_end) = $self->_pre_rangei($other, $rel); |
|
423
|
|
|
|
|
|
|
|
|
424
|
3
|
|
|
|
|
11
|
return $own_end < $other_start; |
|
425
|
|
|
|
|
|
|
} |
|
426
|
|
|
|
|
|
|
|
|
427
|
|
|
|
|
|
|
=head2 greater_than |
|
428
|
|
|
|
|
|
|
|
|
429
|
|
|
|
|
|
|
Title : greater_than |
|
430
|
|
|
|
|
|
|
Usage : if ($position->greater_than($other_position)) {...} |
|
431
|
|
|
|
|
|
|
Function: Ask if this Position starts after another ends. |
|
432
|
|
|
|
|
|
|
Returns : boolean |
|
433
|
|
|
|
|
|
|
Args : arg #1 = a Bio::RangeI (eg. a Bio::Map::Position) to compare this |
|
434
|
|
|
|
|
|
|
one to (mandatory) |
|
435
|
|
|
|
|
|
|
arg #2 = optional Bio::Map::RelativeI to ask if the Position is |
|
436
|
|
|
|
|
|
|
greater in terms of their relative position to the thing |
|
437
|
|
|
|
|
|
|
described by that Relative |
|
438
|
|
|
|
|
|
|
|
|
439
|
|
|
|
|
|
|
=cut |
|
440
|
|
|
|
|
|
|
|
|
441
|
|
|
|
|
|
|
sub greater_than { |
|
442
|
4
|
|
|
4
|
1
|
7
|
my ($self, $other, $rel) = @_; |
|
443
|
|
|
|
|
|
|
|
|
444
|
4
|
|
|
|
|
9
|
my ($own_start, $own_end) = $self->_pre_rangei($self, $rel); |
|
445
|
4
|
|
|
|
|
8
|
my ($other_start, $other_end) = $self->_pre_rangei($other, $rel); |
|
446
|
|
|
|
|
|
|
|
|
447
|
4
|
|
|
|
|
10
|
return $own_start > $other_end; |
|
448
|
|
|
|
|
|
|
} |
|
449
|
|
|
|
|
|
|
|
|
450
|
|
|
|
|
|
|
=head2 overlaps |
|
451
|
|
|
|
|
|
|
|
|
452
|
|
|
|
|
|
|
Title : overlaps |
|
453
|
|
|
|
|
|
|
Usage : if ($p1->overlaps($p2)) {...} |
|
454
|
|
|
|
|
|
|
Function: Tests if $p1 overlaps $p2. |
|
455
|
|
|
|
|
|
|
Returns : True if the positions overlap (regardless of map), false otherwise |
|
456
|
|
|
|
|
|
|
Args : arg #1 = a Bio::RangeI (eg. a Bio::Map::Position) to compare this |
|
457
|
|
|
|
|
|
|
one to (mandatory) |
|
458
|
|
|
|
|
|
|
arg #2 = optional strand-testing arg ('strong', 'weak', 'ignore') |
|
459
|
|
|
|
|
|
|
arg #3 = optional Bio::Map::RelativeI to ask if the Positions |
|
460
|
|
|
|
|
|
|
overlap in terms of their relative position to the thing |
|
461
|
|
|
|
|
|
|
described by that Relative |
|
462
|
|
|
|
|
|
|
arg #4 = optional minimum percentage length of the overlap before |
|
463
|
|
|
|
|
|
|
reporting an overlap exists (default 0) |
|
464
|
|
|
|
|
|
|
|
|
465
|
|
|
|
|
|
|
=cut |
|
466
|
|
|
|
|
|
|
|
|
467
|
|
|
|
|
|
|
sub overlaps { |
|
468
|
|
|
|
|
|
|
# overriding the RangeI implementation so we can handle Relative |
|
469
|
171
|
|
|
171
|
1
|
177
|
my ($self, $other, $so, $rel, $min_percent) = @_; |
|
470
|
171
|
|
50
|
|
|
406
|
$min_percent ||= 0; |
|
471
|
|
|
|
|
|
|
|
|
472
|
171
|
|
|
|
|
143
|
my ($own_min, $other_min) = (0, 0); |
|
473
|
171
|
50
|
|
|
|
221
|
if ($min_percent > 0) { |
|
474
|
0
|
|
|
|
|
0
|
$own_min = (($self->length / 100) * $min_percent) - 1; |
|
475
|
0
|
|
|
|
|
0
|
$other_min = (($other->length / 100) * $min_percent) - 1; |
|
476
|
|
|
|
|
|
|
} |
|
477
|
|
|
|
|
|
|
|
|
478
|
171
|
|
|
|
|
211
|
my ($own_start, $own_end) = $self->_pre_rangei($self, $rel); |
|
479
|
171
|
|
|
|
|
248
|
my ($other_start, $other_end) = $self->_pre_rangei($other, $rel); |
|
480
|
|
|
|
|
|
|
|
|
481
|
171
|
|
66
|
|
|
342
|
return ($self->_testStrand($other, $so) and not |
|
482
|
|
|
|
|
|
|
(($own_start + $own_min > $other_end or $own_end - $own_min < $other_start) || |
|
483
|
|
|
|
|
|
|
($own_start > $other_end - $other_min or $own_end < $other_start + $other_min))); |
|
484
|
|
|
|
|
|
|
} |
|
485
|
|
|
|
|
|
|
|
|
486
|
|
|
|
|
|
|
=head2 contains |
|
487
|
|
|
|
|
|
|
|
|
488
|
|
|
|
|
|
|
Title : contains |
|
489
|
|
|
|
|
|
|
Usage : if ($p1->contains($p2)) {...} |
|
490
|
|
|
|
|
|
|
Function: Tests whether $p1 totally contains $p2. |
|
491
|
|
|
|
|
|
|
Returns : true if the argument is totally contained within this position |
|
492
|
|
|
|
|
|
|
(regardless of map), false otherwise |
|
493
|
|
|
|
|
|
|
Args : arg #1 = a Bio::RangeI (eg. a Bio::Map::Position) to compare this |
|
494
|
|
|
|
|
|
|
one to, or scalar number (mandatory) |
|
495
|
|
|
|
|
|
|
arg #2 = optional strand-testing arg ('strong', 'weak', 'ignore') |
|
496
|
|
|
|
|
|
|
arg #3 = optional Bio::Map::RelativeI to ask if the Position |
|
497
|
|
|
|
|
|
|
is contained in terms of their relative position to the |
|
498
|
|
|
|
|
|
|
thing described by that Relative |
|
499
|
|
|
|
|
|
|
|
|
500
|
|
|
|
|
|
|
=cut |
|
501
|
|
|
|
|
|
|
|
|
502
|
|
|
|
|
|
|
sub contains { |
|
503
|
|
|
|
|
|
|
# overriding the RangeI implementation so we can handle Relative |
|
504
|
12
|
|
|
12
|
1
|
18
|
my ($self, $other, $so, $rel) = @_; |
|
505
|
|
|
|
|
|
|
|
|
506
|
12
|
|
|
|
|
32
|
my ($own_start, $own_end) = $self->_pre_rangei($self, $rel); |
|
507
|
12
|
|
|
|
|
25
|
my ($other_start, $other_end) = $self->_pre_rangei($other, $rel); |
|
508
|
|
|
|
|
|
|
|
|
509
|
12
|
|
100
|
|
|
39
|
return ($self->_testStrand($other, $so) and |
|
510
|
|
|
|
|
|
|
$other_start >= $own_start and $other_end <= $own_end); |
|
511
|
|
|
|
|
|
|
} |
|
512
|
|
|
|
|
|
|
|
|
513
|
|
|
|
|
|
|
=head2 intersection |
|
514
|
|
|
|
|
|
|
|
|
515
|
|
|
|
|
|
|
Title : intersection |
|
516
|
|
|
|
|
|
|
Usage : ($start, $stop, $strand) = $p1->intersection($p2) |
|
517
|
|
|
|
|
|
|
($start, $stop, $strand) = Bio::Map::Position->intersection(\@positions); |
|
518
|
|
|
|
|
|
|
$mappable = $p1->intersection($p2, undef, $relative); |
|
519
|
|
|
|
|
|
|
$mappable = Bio::Map::Position->intersection(\@positions); |
|
520
|
|
|
|
|
|
|
Function: gives the range that is contained by all ranges |
|
521
|
|
|
|
|
|
|
Returns : undef if they do not overlap, OR |
|
522
|
|
|
|
|
|
|
Bio::Map::Mappable object who's positions are the |
|
523
|
|
|
|
|
|
|
cross-map-calculated intersection of the input positions on all the |
|
524
|
|
|
|
|
|
|
maps that the input positions belong to, OR, in list context, a three |
|
525
|
|
|
|
|
|
|
element array (start, end, strand) |
|
526
|
|
|
|
|
|
|
Args : arg #1 = [REQUIRED] a Bio::RangeI (eg. a Bio::Map::Position) to |
|
527
|
|
|
|
|
|
|
compare this one to, or an array ref of Bio::RangeI |
|
528
|
|
|
|
|
|
|
arg #2 = optional strand-testing arg ('strong', 'weak', 'ignore') |
|
529
|
|
|
|
|
|
|
arg #3 = optional Bio::Map::RelativeI to ask how the Positions |
|
530
|
|
|
|
|
|
|
intersect in terms of their relative position to the thing |
|
531
|
|
|
|
|
|
|
described by that Relative |
|
532
|
|
|
|
|
|
|
|
|
533
|
|
|
|
|
|
|
=cut |
|
534
|
|
|
|
|
|
|
|
|
535
|
|
|
|
|
|
|
sub intersection { |
|
536
|
|
|
|
|
|
|
# overriding the RangeI implementation so we can transfer map and handle |
|
537
|
|
|
|
|
|
|
# Relative |
|
538
|
4
|
|
|
4
|
1
|
8
|
my ($self, $given, $so, $rel) = @_; |
|
539
|
4
|
50
|
|
|
|
12
|
$self->throw("missing arg: you need to pass in another argument") unless $given; |
|
540
|
|
|
|
|
|
|
|
|
541
|
4
|
|
|
|
|
5
|
my @positions; |
|
542
|
4
|
50
|
|
|
|
14
|
if ($self eq "Bio::Map::PositionI") { |
|
543
|
0
|
|
|
|
|
0
|
$self = "Bio::Map::Position"; |
|
544
|
0
|
|
|
|
|
0
|
$self->warn("calling static methods of an interface is deprecated; use $self instead"); |
|
545
|
|
|
|
|
|
|
} |
|
546
|
4
|
50
|
|
|
|
10
|
if (ref $self) { |
|
547
|
4
|
|
|
|
|
5
|
push(@positions, $self); |
|
548
|
|
|
|
|
|
|
} |
|
549
|
4
|
100
|
|
|
|
12
|
ref($given) eq 'ARRAY' ? push(@positions, @{$given}) : push(@positions, $given); |
|
|
2
|
|
|
|
|
4
|
|
|
550
|
4
|
50
|
|
|
|
10
|
$self->throw("Need at least 2 Positions") unless @positions >= 2; |
|
551
|
|
|
|
|
|
|
|
|
552
|
4
|
|
|
|
|
5
|
my ($intersect, $i_start, $i_end, $c_start, $c_end, %known_maps); |
|
553
|
4
|
|
|
|
|
13
|
while (@positions > 0) { |
|
554
|
12
|
100
|
|
|
|
20
|
unless ($intersect) { |
|
555
|
4
|
|
|
|
|
7
|
$intersect = shift(@positions); |
|
556
|
4
|
|
|
|
|
9
|
($i_start, $i_end) = $self->_pre_rangei($intersect, $rel); |
|
557
|
4
|
|
|
|
|
11
|
my $map = $intersect->map; |
|
558
|
4
|
|
|
|
|
15
|
$known_maps{$map->unique_id} = $map; |
|
559
|
|
|
|
|
|
|
} |
|
560
|
|
|
|
|
|
|
|
|
561
|
12
|
|
|
|
|
15
|
my $compare = shift(@positions); |
|
562
|
12
|
|
|
|
|
22
|
($c_start, $c_end) = $self->_pre_rangei($compare, $rel); |
|
563
|
12
|
50
|
|
|
|
30
|
return unless $compare->_testStrand($intersect, $so); |
|
564
|
12
|
50
|
|
|
|
26
|
if ($compare->isa('Bio::Map::PositionI')) { |
|
565
|
12
|
|
|
|
|
15
|
my $this_map = $compare->map; |
|
566
|
12
|
50
|
|
|
|
21
|
if ($this_map) { |
|
567
|
12
|
|
|
|
|
23
|
$known_maps{$this_map->unique_id} = $this_map; |
|
568
|
|
|
|
|
|
|
} |
|
569
|
|
|
|
|
|
|
} |
|
570
|
|
|
|
|
|
|
else { |
|
571
|
0
|
|
|
|
|
0
|
$self->throw("Only Bio::Map::PositionI objects are supported, not [$compare]"); |
|
572
|
|
|
|
|
|
|
} |
|
573
|
|
|
|
|
|
|
|
|
574
|
12
|
|
|
|
|
35
|
my @starts = sort {$a <=> $b} ($i_start, $c_start); |
|
|
12
|
|
|
|
|
24
|
|
|
575
|
12
|
|
|
|
|
16
|
my @ends = sort {$a <=> $b} ($i_end, $c_end); |
|
|
12
|
|
|
|
|
19
|
|
|
576
|
|
|
|
|
|
|
|
|
577
|
12
|
|
|
|
|
13
|
my $start = pop @starts; # larger of the 2 starts |
|
578
|
12
|
|
|
|
|
11
|
my $end = shift @ends; # smaller of the 2 ends |
|
579
|
|
|
|
|
|
|
|
|
580
|
12
|
|
|
|
|
9
|
my $intersect_strand; # strand for the intersection |
|
581
|
12
|
50
|
33
|
|
|
31
|
if (defined($intersect->strand) && defined($compare->strand) && $intersect->strand == $compare->strand) { |
|
|
|
|
33
|
|
|
|
|
|
582
|
12
|
|
|
|
|
17
|
$intersect_strand = $compare->strand; |
|
583
|
|
|
|
|
|
|
} |
|
584
|
|
|
|
|
|
|
else { |
|
585
|
0
|
|
|
|
|
0
|
$intersect_strand = 0; |
|
586
|
|
|
|
|
|
|
} |
|
587
|
|
|
|
|
|
|
|
|
588
|
12
|
50
|
|
|
|
20
|
if ($start > $end) { |
|
589
|
0
|
|
|
|
|
0
|
return; |
|
590
|
|
|
|
|
|
|
} |
|
591
|
|
|
|
|
|
|
else { |
|
592
|
12
|
|
|
|
|
40
|
$intersect = $self->new(-start => $start, |
|
593
|
|
|
|
|
|
|
-end => $end, |
|
594
|
|
|
|
|
|
|
-strand => $intersect_strand); |
|
595
|
|
|
|
|
|
|
} |
|
596
|
|
|
|
|
|
|
} |
|
597
|
|
|
|
|
|
|
|
|
598
|
4
|
50
|
|
|
|
10
|
$intersect || return; |
|
599
|
4
|
|
|
|
|
9
|
my ($start, $end, $strand) = ($intersect->start, $intersect->end, $intersect->strand); |
|
600
|
|
|
|
|
|
|
|
|
601
|
4
|
|
|
|
|
6
|
my @intersects; |
|
602
|
4
|
|
|
|
|
13
|
foreach my $known_map (values %known_maps) { |
|
603
|
9
|
|
|
|
|
31
|
my $new_intersect = $intersect->new(-start => $start, |
|
604
|
|
|
|
|
|
|
-end => $end, |
|
605
|
|
|
|
|
|
|
-strand => $strand, |
|
606
|
|
|
|
|
|
|
-map => $known_map); |
|
607
|
9
|
100
|
|
|
|
24
|
$new_intersect->relative($rel) if $rel; |
|
608
|
9
|
|
|
|
|
16
|
push(@intersects, $new_intersect); |
|
609
|
|
|
|
|
|
|
} |
|
610
|
4
|
50
|
|
|
|
9
|
unless (@intersects) { |
|
611
|
0
|
0
|
|
|
|
0
|
$intersect->relative($rel) if $rel; |
|
612
|
0
|
|
|
|
|
0
|
@intersects = ($intersect); |
|
613
|
|
|
|
|
|
|
} |
|
614
|
|
|
|
|
|
|
|
|
615
|
4
|
|
|
|
|
17
|
my $result = Bio::Map::Mappable->new(); |
|
616
|
4
|
|
|
|
|
20
|
$result->add_position(@intersects); # sneaky, add_position can take a list of positions |
|
617
|
4
|
|
|
|
|
22
|
return $result; |
|
618
|
|
|
|
|
|
|
} |
|
619
|
|
|
|
|
|
|
|
|
620
|
|
|
|
|
|
|
=head2 union |
|
621
|
|
|
|
|
|
|
|
|
622
|
|
|
|
|
|
|
Title : union |
|
623
|
|
|
|
|
|
|
Usage : ($start, $stop, $strand) = $p1->union($p2); |
|
624
|
|
|
|
|
|
|
($start, $stop, $strand) = Bio::Map::Position->union(@positions); |
|
625
|
|
|
|
|
|
|
my $mappable = $p1->union($p2); |
|
626
|
|
|
|
|
|
|
my $mappable = Bio::Map::Position->union(@positions); |
|
627
|
|
|
|
|
|
|
Function: finds the minimal position/range that contains all of the positions |
|
628
|
|
|
|
|
|
|
Returns : Bio::Map::Mappable object who's positions are the |
|
629
|
|
|
|
|
|
|
cross-map-calculated union of the input positions on all the maps |
|
630
|
|
|
|
|
|
|
that the input positions belong to, OR, in list context, a three |
|
631
|
|
|
|
|
|
|
element array (start, end, strand) |
|
632
|
|
|
|
|
|
|
Args : a Bio::Map::PositionI to compare this one to, or a list of such |
|
633
|
|
|
|
|
|
|
OR |
|
634
|
|
|
|
|
|
|
a single Bio::Map::PositionI or array ref of such AND a |
|
635
|
|
|
|
|
|
|
Bio::Map::RelativeI to ask for the Position's union in terms of their |
|
636
|
|
|
|
|
|
|
relative position to the thing described by that Relative |
|
637
|
|
|
|
|
|
|
|
|
638
|
|
|
|
|
|
|
=cut |
|
639
|
|
|
|
|
|
|
|
|
640
|
|
|
|
|
|
|
sub union { |
|
641
|
|
|
|
|
|
|
# overriding the RangeI implementation so we can transfer map and handle |
|
642
|
|
|
|
|
|
|
# Relative |
|
643
|
38
|
|
|
38
|
1
|
57
|
my ($self, @args) = @_; |
|
644
|
38
|
50
|
|
|
|
62
|
$self->throw("Not enough arguments") unless @args >= 1; |
|
645
|
|
|
|
|
|
|
|
|
646
|
38
|
|
|
|
|
40
|
my @positions; |
|
647
|
|
|
|
|
|
|
my $rel; |
|
648
|
38
|
50
|
|
|
|
69
|
if ($self eq "Bio::Map::PositionI") { |
|
649
|
0
|
|
|
|
|
0
|
$self = "Bio::Map::Position"; |
|
650
|
0
|
|
|
|
|
0
|
$self->warn("calling static methods of an interface is deprecated; use $self instead"); |
|
651
|
|
|
|
|
|
|
} |
|
652
|
38
|
50
|
|
|
|
63
|
if (ref $self) { |
|
653
|
38
|
|
|
|
|
41
|
push(@positions, $self); |
|
654
|
|
|
|
|
|
|
} |
|
655
|
38
|
100
|
|
|
|
60
|
if (ref $args[0] eq 'ARRAY') { |
|
656
|
1
|
|
|
|
|
2
|
push(@positions, @{shift(@args)}); |
|
|
1
|
|
|
|
|
1
|
|
|
657
|
|
|
|
|
|
|
} |
|
658
|
|
|
|
|
|
|
else { |
|
659
|
37
|
|
|
|
|
41
|
push(@positions, shift(@args)); |
|
660
|
|
|
|
|
|
|
} |
|
661
|
38
|
100
|
66
|
|
|
156
|
if ($args[0] && $args[0]->isa('Bio::Map::RelativeI')) { |
|
662
|
37
|
|
|
|
|
36
|
$rel = shift(@args); |
|
663
|
|
|
|
|
|
|
} |
|
664
|
38
|
|
|
|
|
64
|
foreach my $arg (@args) { |
|
665
|
|
|
|
|
|
|
# avoid pushing undefined values into @positions |
|
666
|
0
|
0
|
|
|
|
0
|
push(@positions, $arg) if $arg; |
|
667
|
|
|
|
|
|
|
} |
|
668
|
38
|
50
|
|
|
|
61
|
$self->throw("Need at least 2 Positions") unless @positions >= 2; |
|
669
|
|
|
|
|
|
|
|
|
670
|
38
|
|
|
|
|
24
|
my (@starts, @ends, %known_maps, $union_strand); |
|
671
|
38
|
|
|
|
|
36
|
foreach my $compare (@positions) { |
|
672
|
|
|
|
|
|
|
# RangeI union allows start or end to be undefined; however _pre_rangei |
|
673
|
|
|
|
|
|
|
# will throw |
|
674
|
78
|
|
|
|
|
95
|
my ($start, $end) = $self->_pre_rangei($compare, $rel); |
|
675
|
|
|
|
|
|
|
|
|
676
|
78
|
50
|
|
|
|
167
|
if ($compare->isa('Bio::Map::PositionI')) { |
|
677
|
78
|
|
|
|
|
93
|
my $this_map = $compare->map; |
|
678
|
78
|
50
|
|
|
|
126
|
if ($this_map) { |
|
679
|
78
|
|
|
|
|
123
|
$known_maps{$this_map->unique_id} = $this_map; |
|
680
|
|
|
|
|
|
|
} |
|
681
|
|
|
|
|
|
|
} |
|
682
|
|
|
|
|
|
|
else { |
|
683
|
0
|
|
|
|
|
0
|
$self->throw("Only Bio::Map::PositionI objects are supported, not [$compare]"); |
|
684
|
|
|
|
|
|
|
} |
|
685
|
|
|
|
|
|
|
|
|
686
|
78
|
100
|
|
|
|
109
|
if (! defined $union_strand) { |
|
687
|
38
|
|
|
|
|
55
|
$union_strand = $compare->strand; |
|
688
|
|
|
|
|
|
|
} |
|
689
|
|
|
|
|
|
|
else { |
|
690
|
40
|
50
|
33
|
|
|
52
|
if (! defined $compare->strand or $union_strand ne $compare->strand) { |
|
691
|
0
|
|
|
|
|
0
|
$union_strand = 0; |
|
692
|
|
|
|
|
|
|
} |
|
693
|
|
|
|
|
|
|
} |
|
694
|
|
|
|
|
|
|
|
|
695
|
78
|
|
|
|
|
84
|
push(@starts, $start); |
|
696
|
78
|
|
|
|
|
92
|
push(@ends, $end); |
|
697
|
|
|
|
|
|
|
} |
|
698
|
|
|
|
|
|
|
|
|
699
|
38
|
|
|
|
|
91
|
@starts = sort { $a <=> $b } @starts; |
|
|
41
|
|
|
|
|
58
|
|
|
700
|
38
|
|
|
|
|
31
|
@ends = sort { $a <=> $b } @ends; |
|
|
41
|
|
|
|
|
35
|
|
|
701
|
38
|
|
|
|
|
31
|
my $start = shift @starts; |
|
702
|
38
|
|
|
|
|
32
|
my $end = pop @ends; |
|
703
|
|
|
|
|
|
|
|
|
704
|
38
|
|
|
|
|
31
|
my @unions; |
|
705
|
38
|
|
|
|
|
53
|
foreach my $known_map (values %known_maps) { |
|
706
|
45
|
|
|
|
|
135
|
my $new_union = $self->new(-start => $start, |
|
707
|
|
|
|
|
|
|
-end => $end, |
|
708
|
|
|
|
|
|
|
-strand => $union_strand, |
|
709
|
|
|
|
|
|
|
-map => $known_map); |
|
710
|
45
|
100
|
|
|
|
110
|
$new_union->relative($rel) if $rel; |
|
711
|
45
|
|
|
|
|
66
|
push(@unions, $new_union); |
|
712
|
|
|
|
|
|
|
} |
|
713
|
38
|
50
|
|
|
|
56
|
unless (@unions) { |
|
714
|
0
|
|
|
|
|
0
|
@unions = ($self->new(-start => $start, |
|
715
|
|
|
|
|
|
|
-end => $end, |
|
716
|
|
|
|
|
|
|
-strand => $union_strand)); |
|
717
|
0
|
0
|
|
|
|
0
|
$unions[0]->relative($rel) if $rel; |
|
718
|
|
|
|
|
|
|
} |
|
719
|
|
|
|
|
|
|
|
|
720
|
38
|
|
|
|
|
78
|
my $result = Bio::Map::Mappable->new(); |
|
721
|
38
|
|
|
|
|
68
|
$result->add_position(@unions); # sneaky, add_position can take a list of positions |
|
722
|
38
|
|
|
|
|
102
|
return $result; |
|
723
|
|
|
|
|
|
|
} |
|
724
|
|
|
|
|
|
|
|
|
725
|
|
|
|
|
|
|
=head2 overlap_extent |
|
726
|
|
|
|
|
|
|
|
|
727
|
|
|
|
|
|
|
Title : overlap_extent |
|
728
|
|
|
|
|
|
|
Usage : ($a_unique,$common,$b_unique) = $a->overlap_extent($b) |
|
729
|
|
|
|
|
|
|
Function: Provides actual amount of overlap between two different |
|
730
|
|
|
|
|
|
|
positions |
|
731
|
|
|
|
|
|
|
Example : |
|
732
|
|
|
|
|
|
|
Returns : array of values containing the length unique to the calling |
|
733
|
|
|
|
|
|
|
position, the length common to both, and the length unique to |
|
734
|
|
|
|
|
|
|
the argument position |
|
735
|
|
|
|
|
|
|
Args : a position |
|
736
|
|
|
|
|
|
|
|
|
737
|
|
|
|
|
|
|
=cut |
|
738
|
|
|
|
|
|
|
|
|
739
|
|
|
|
|
|
|
#*** should this be overridden from RangeI? |
|
740
|
|
|
|
|
|
|
|
|
741
|
|
|
|
|
|
|
=head2 disconnected_ranges |
|
742
|
|
|
|
|
|
|
|
|
743
|
|
|
|
|
|
|
Title : disconnected_ranges |
|
744
|
|
|
|
|
|
|
Usage : my @disc_ranges = Bio::Map::Position->disconnected_ranges(@ranges); |
|
745
|
|
|
|
|
|
|
Function: Creates the minimal set of positions such that each input position is |
|
746
|
|
|
|
|
|
|
fully contained by at least one output position, and none of the |
|
747
|
|
|
|
|
|
|
output positions overlap. |
|
748
|
|
|
|
|
|
|
Returns : Bio::Map::Mappable with the calculated disconnected ranges |
|
749
|
|
|
|
|
|
|
Args : a Bio::Map::PositionI to compare this one to, or a list of such, |
|
750
|
|
|
|
|
|
|
OR |
|
751
|
|
|
|
|
|
|
a single Bio::Map::PositionI or array ref of such AND a |
|
752
|
|
|
|
|
|
|
Bio::Map::RelativeI to consider all Position's co-ordinates in terms |
|
753
|
|
|
|
|
|
|
of their relative position to the thing described by that Relative, |
|
754
|
|
|
|
|
|
|
AND, optionally, an int for the minimum percentage of overlap that |
|
755
|
|
|
|
|
|
|
must be present before considering two ranges to be overlapping |
|
756
|
|
|
|
|
|
|
(default 0) |
|
757
|
|
|
|
|
|
|
|
|
758
|
|
|
|
|
|
|
=cut |
|
759
|
|
|
|
|
|
|
|
|
760
|
|
|
|
|
|
|
sub disconnected_ranges { |
|
761
|
|
|
|
|
|
|
# overriding the RangeI implementation so we can transfer map and handle |
|
762
|
|
|
|
|
|
|
# Relative |
|
763
|
10
|
|
|
10
|
1
|
12
|
my ($self, @args) = @_; |
|
764
|
10
|
50
|
|
|
|
18
|
$self->throw("Not enough arguments") unless @args >= 1; |
|
765
|
|
|
|
|
|
|
|
|
766
|
10
|
|
|
|
|
9
|
my @positions; |
|
767
|
|
|
|
|
|
|
my $rel; |
|
768
|
10
|
|
|
|
|
12
|
my $overlap = 0; |
|
769
|
10
|
50
|
|
|
|
24
|
if ($self eq "Bio::Map::PositionI") { |
|
770
|
0
|
|
|
|
|
0
|
$self = "Bio::Map::Position"; |
|
771
|
0
|
|
|
|
|
0
|
$self->warn("calling static methods of an interface is deprecated; use $self instead"); |
|
772
|
|
|
|
|
|
|
} |
|
773
|
10
|
50
|
|
|
|
21
|
if (ref $self) { |
|
774
|
10
|
|
|
|
|
10
|
push(@positions, $self); |
|
775
|
|
|
|
|
|
|
} |
|
776
|
10
|
50
|
|
|
|
22
|
if (ref $args[0] eq 'ARRAY') { |
|
777
|
10
|
|
|
|
|
10
|
push(@positions, @{shift(@args)}); |
|
|
10
|
|
|
|
|
18
|
|
|
778
|
|
|
|
|
|
|
} |
|
779
|
|
|
|
|
|
|
else { |
|
780
|
0
|
|
|
|
|
0
|
push(@positions, shift(@args)); |
|
781
|
|
|
|
|
|
|
} |
|
782
|
10
|
50
|
33
|
|
|
54
|
if ($args[0] && $args[0]->isa('Bio::Map::RelativeI')) { |
|
783
|
10
|
|
|
|
|
9
|
$rel = shift(@args); |
|
784
|
10
|
|
|
|
|
11
|
$overlap = shift(@args); |
|
785
|
|
|
|
|
|
|
} |
|
786
|
10
|
|
|
|
|
18
|
foreach my $arg (@args) { |
|
787
|
0
|
0
|
|
|
|
0
|
push(@positions, $arg) if $arg; |
|
788
|
|
|
|
|
|
|
} |
|
789
|
10
|
50
|
|
|
|
17
|
$self->throw("Need at least 2 Positions") unless @positions >= 2; |
|
790
|
|
|
|
|
|
|
|
|
791
|
10
|
|
|
|
|
9
|
my %known_maps; |
|
792
|
10
|
|
|
|
|
15
|
foreach my $pos (@positions) { |
|
793
|
55
|
50
|
|
|
|
98
|
$pos->isa('Bio::Map::PositionI') || $self->throw("Must supply only Bio::Map::PositionI objects, not [$pos]"); |
|
794
|
55
|
|
50
|
|
|
56
|
my $map = $pos->map || next; |
|
795
|
55
|
|
|
|
|
82
|
$known_maps{$map->unique_id} = $map; |
|
796
|
|
|
|
|
|
|
} |
|
797
|
10
|
|
|
|
|
9
|
my %prior_positions; |
|
798
|
10
|
|
|
|
|
16
|
foreach my $map (values %known_maps) { |
|
799
|
15
|
|
|
|
|
40
|
foreach my $pos ($map->get_positions) { |
|
800
|
92
|
|
|
|
|
127
|
$prior_positions{$pos} = 1; |
|
801
|
|
|
|
|
|
|
} |
|
802
|
|
|
|
|
|
|
} |
|
803
|
|
|
|
|
|
|
|
|
804
|
10
|
|
|
|
|
15
|
my @outranges = (); |
|
805
|
10
|
|
|
|
|
14
|
foreach my $inrange (@positions) { |
|
806
|
55
|
|
|
|
|
49
|
my @outranges_new = (); |
|
807
|
55
|
|
|
|
|
53
|
my %overlapping_ranges = (); |
|
808
|
|
|
|
|
|
|
|
|
809
|
55
|
|
|
|
|
97
|
for (my $i=0; $i<@outranges; $i++) { |
|
810
|
50
|
|
|
|
|
48
|
my $outrange = $outranges[$i]; |
|
811
|
50
|
100
|
|
|
|
102
|
if ($inrange->overlaps($outrange, undef, $rel, $overlap)) { |
|
812
|
35
|
|
|
|
|
61
|
my $union_able = $inrange->union($outrange, $rel); # using $inrange->union($outrange, $rel); gives >6x speedup, |
|
813
|
|
|
|
|
|
|
# but different answer, not necessarily incorrect... |
|
814
|
35
|
|
|
|
|
65
|
foreach my $pos ($union_able->get_positions) { |
|
815
|
35
|
|
|
|
|
62
|
$overlapping_ranges{$pos->toString} = $pos; # we flatten down to a result on a single map |
|
816
|
|
|
|
|
|
|
# to avoid creating 10s of thousands of positions during this process; |
|
817
|
|
|
|
|
|
|
# we then apply the final answer to all maps at the very end |
|
818
|
35
|
|
|
|
|
107
|
last; |
|
819
|
|
|
|
|
|
|
} |
|
820
|
|
|
|
|
|
|
} |
|
821
|
|
|
|
|
|
|
else { |
|
822
|
15
|
|
|
|
|
42
|
push(@outranges_new, $outrange); |
|
823
|
|
|
|
|
|
|
} |
|
824
|
|
|
|
|
|
|
} |
|
825
|
|
|
|
|
|
|
|
|
826
|
55
|
|
|
|
|
68
|
@outranges = @outranges_new; |
|
827
|
|
|
|
|
|
|
|
|
828
|
55
|
|
|
|
|
67
|
my @overlappers = values %overlapping_ranges; |
|
829
|
55
|
100
|
|
|
|
68
|
if (@overlappers) { |
|
830
|
35
|
50
|
|
|
|
42
|
if (@overlappers > 1) { |
|
831
|
0
|
|
|
|
|
0
|
my $merged_range_able = shift(@overlappers)->union(\@overlappers, $rel); |
|
832
|
0
|
|
|
|
|
0
|
push(@outranges, $merged_range_able->get_positions); |
|
833
|
|
|
|
|
|
|
} |
|
834
|
|
|
|
|
|
|
else { |
|
835
|
35
|
|
|
|
|
74
|
push(@outranges, @overlappers); |
|
836
|
|
|
|
|
|
|
} |
|
837
|
|
|
|
|
|
|
} |
|
838
|
|
|
|
|
|
|
else { |
|
839
|
20
|
|
|
|
|
47
|
push(@outranges, $self->new(-start => $inrange->start($rel), -end => $inrange->end($rel), -strand => $inrange->strand, -map => $inrange->map, -relative => $rel)); |
|
840
|
|
|
|
|
|
|
} |
|
841
|
|
|
|
|
|
|
} |
|
842
|
|
|
|
|
|
|
|
|
843
|
|
|
|
|
|
|
# purge positions that were created whilst calculating the answer, but |
|
844
|
|
|
|
|
|
|
# aren't the final answer and weren't there previously |
|
845
|
10
|
|
|
|
|
15
|
my %answers = map { $_ => 1 } @outranges; |
|
|
20
|
|
|
|
|
48
|
|
|
846
|
10
|
|
|
|
|
23
|
foreach my $map (values %known_maps) { |
|
847
|
15
|
|
|
|
|
31
|
foreach my $pos ($map->get_positions) { |
|
848
|
154
|
100
|
66
|
|
|
322
|
if (! exists $prior_positions{$pos} && ! exists $answers{$pos}) { |
|
849
|
42
|
|
|
|
|
86
|
$map->purge_positions($pos); |
|
850
|
|
|
|
|
|
|
} |
|
851
|
|
|
|
|
|
|
} |
|
852
|
|
|
|
|
|
|
} |
|
853
|
|
|
|
|
|
|
|
|
854
|
10
|
|
|
|
|
12
|
my %post_positions; |
|
855
|
10
|
|
|
|
|
19
|
foreach my $map (values %known_maps) { |
|
856
|
15
|
|
|
|
|
27
|
foreach my $pos ($map->get_positions) { |
|
857
|
112
|
|
|
|
|
146
|
$post_positions{$pos} = 1; |
|
858
|
|
|
|
|
|
|
} |
|
859
|
|
|
|
|
|
|
} |
|
860
|
|
|
|
|
|
|
|
|
861
|
10
|
50
|
|
|
|
22
|
@outranges || return; |
|
862
|
|
|
|
|
|
|
|
|
863
|
|
|
|
|
|
|
# make an outrange on all known maps |
|
864
|
10
|
|
|
|
|
12
|
my @final_positions; |
|
865
|
10
|
|
|
|
|
22
|
foreach my $map (values %known_maps) { |
|
866
|
15
|
|
|
|
|
20
|
foreach my $pos (@outranges) { |
|
867
|
30
|
100
|
|
|
|
45
|
if ($pos->map eq $map) { |
|
868
|
20
|
|
|
|
|
34
|
push(@final_positions, $pos); |
|
869
|
|
|
|
|
|
|
} |
|
870
|
|
|
|
|
|
|
else { |
|
871
|
10
|
|
|
|
|
22
|
push(@final_positions, $pos->new(-start => $pos->start, |
|
872
|
|
|
|
|
|
|
-end => $pos->end, |
|
873
|
|
|
|
|
|
|
-relative => $pos->relative, |
|
874
|
|
|
|
|
|
|
-map => $map)); |
|
875
|
|
|
|
|
|
|
} |
|
876
|
|
|
|
|
|
|
} |
|
877
|
|
|
|
|
|
|
} |
|
878
|
|
|
|
|
|
|
|
|
879
|
|
|
|
|
|
|
# assign the positions to a result mappable |
|
880
|
10
|
|
|
|
|
27
|
my $result = Bio::Map::Mappable->new(); |
|
881
|
10
|
|
|
|
|
21
|
$result->add_position(@final_positions); # sneaky, add_position can take a list of positions |
|
882
|
10
|
|
|
|
|
66
|
return $result; |
|
883
|
|
|
|
|
|
|
} |
|
884
|
|
|
|
|
|
|
|
|
885
|
|
|
|
|
|
|
# get start & end suitable for rangeI methods, taking relative into account |
|
886
|
|
|
|
|
|
|
sub _pre_rangei { |
|
887
|
490
|
|
|
490
|
|
514
|
my ($self, $other, $rel) = @_; |
|
888
|
490
|
50
|
|
|
|
648
|
$self->throw("Must supply an object") unless $other; |
|
889
|
490
|
100
|
|
|
|
740
|
if ($rel) { |
|
890
|
414
|
50
|
|
|
|
555
|
$self->throw("Must supply an object for the Relative argument") unless ref($rel); |
|
891
|
414
|
50
|
|
|
|
854
|
$self->throw("This is [$rel], not a Bio::Map::RelativeI") unless $rel->isa('Bio::Map::RelativeI'); |
|
892
|
|
|
|
|
|
|
} |
|
893
|
|
|
|
|
|
|
|
|
894
|
490
|
|
|
|
|
338
|
my ($other_start, $other_end); |
|
895
|
490
|
100
|
|
|
|
585
|
if (ref($other)) { |
|
896
|
489
|
50
|
|
|
|
651
|
if (ref($other) eq 'ARRAY') { |
|
897
|
0
|
|
|
|
|
0
|
$self->throw("_pre_rangei got an array"); |
|
898
|
|
|
|
|
|
|
} |
|
899
|
489
|
50
|
33
|
|
|
1632
|
$self->throw("This is [$other], not a Bio::RangeI object") unless defined $other && $other->isa('Bio::RangeI'); |
|
900
|
|
|
|
|
|
|
|
|
901
|
489
|
100
|
|
|
|
752
|
if ($other->isa('Bio::Map::PositionI')) { |
|
902
|
|
|
|
|
|
|
# to get the desired start/end we need the position to be on a map; |
|
903
|
|
|
|
|
|
|
# if it isn't on one temporarily place it on self's map |
|
904
|
|
|
|
|
|
|
# - this lets us have 'generic' positions that aren't on any map |
|
905
|
|
|
|
|
|
|
# but have a relative defined and can thus be usefully compared to |
|
906
|
|
|
|
|
|
|
# positions that /are/ on maps |
|
907
|
488
|
|
|
|
|
584
|
my $other_map = $other->map; |
|
908
|
488
|
50
|
|
|
|
798
|
unless ($other_map) { |
|
909
|
0
|
|
0
|
|
|
0
|
my $self_map = $self->map || $self->throw("Trying to compare two positions but neither had been placed on a map"); |
|
910
|
0
|
|
|
|
|
0
|
$other->map($self_map); |
|
911
|
|
|
|
|
|
|
} |
|
912
|
|
|
|
|
|
|
|
|
913
|
|
|
|
|
|
|
# want start and end positions relative to the supplied rel or map start |
|
914
|
488
|
|
66
|
|
|
726
|
$rel ||= $other->absolute_relative; |
|
915
|
488
|
|
|
|
|
760
|
$other_start = $other->start($rel); |
|
916
|
488
|
|
|
|
|
741
|
$other_end = $other->end($rel); |
|
917
|
|
|
|
|
|
|
|
|
918
|
488
|
50
|
|
|
|
789
|
unless ($other_map) { |
|
919
|
0
|
|
|
|
|
0
|
$self->map->purge_positions($other); |
|
920
|
|
|
|
|
|
|
} |
|
921
|
|
|
|
|
|
|
} |
|
922
|
|
|
|
|
|
|
else { |
|
923
|
1
|
|
|
|
|
5
|
$other_start = $other->start; |
|
924
|
1
|
|
|
|
|
4
|
$other_end = $other->end; |
|
925
|
|
|
|
|
|
|
} |
|
926
|
|
|
|
|
|
|
} |
|
927
|
|
|
|
|
|
|
else { |
|
928
|
1
|
50
|
|
|
|
5
|
$self->throw("not a number") unless looks_like_number($other); |
|
929
|
1
|
|
|
|
|
2
|
$other_start = $other_end = $other; |
|
930
|
|
|
|
|
|
|
} |
|
931
|
|
|
|
|
|
|
|
|
932
|
490
|
50
|
|
|
|
555
|
$other->throw("start is undefined") unless defined $other_start; |
|
933
|
490
|
50
|
|
|
|
507
|
$other->throw("end is undefined") unless defined $other_end; |
|
934
|
|
|
|
|
|
|
|
|
935
|
490
|
|
|
|
|
608
|
return ($other_start, $other_end); |
|
936
|
|
|
|
|
|
|
} |
|
937
|
|
|
|
|
|
|
|
|
938
|
|
|
|
|
|
|
1; |