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# BioPerl module for Bio::DB::GenPept |
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# |
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# Please direct questions and support issues to |
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# |
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# Cared for by Aaron Mackey |
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# |
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# Copyright Aaron Mackey |
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# |
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# You may distribute this module under the same terms as perl itself |
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# POD documentation - main docs before the code |
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# completely reworked by Jason Stajich to use Bio::DB::WebDBSeqI 2000-12-12 |
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=head1 NAME |
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Bio::DB::GenPept - Database object interface to GenPept |
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=head1 SYNOPSIS |
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$gb = Bio::DB::GenPept->new(); |
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$seq = $gb->get_Seq_by_id('195055'); # Unique ID |
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# or ... |
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$seq = $gb->get_Seq_by_acc('DEECTH'); # Accession Number |
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my $seqio = $gb->get_Stream_by_id(['195055', 'DEECTH']); |
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while( my $seq = $seqio->next_seq ) { |
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print "seq is is ", $seq->display_id, "\n"; |
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} |
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=head1 DESCRIPTION |
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Allows the dynamic retrieval of Sequence objects (Bio::Seq) from the GenPept |
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database at NCBI, via an Entrez query. |
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WARNING: Please do NOT spam the Entrez web server with multiple requests. |
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NCBI offers Batch Entrez for this purpose. Batch Entrez support will likely |
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be supported in a future version of DB::GenPept. |
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Currently the only return format supported by NCBI Entrez for GenPept |
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database is GenPept format, so any format specification passed to |
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GenPept will be ignored still be forced to GenPept format (which is |
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just GenBank format). |
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=head1 FEEDBACK |
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=head2 Mailing Lists |
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User feedback is an integral part of the |
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evolution of this and other Bioperl modules. Send |
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your comments and suggestions preferably to one |
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of the Bioperl mailing lists. Your participation |
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is much appreciated. |
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bioperl-l@bioperl.org - General discussion |
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http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
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=head2 Support |
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Please direct usage questions or support issues to the mailing list: |
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I |
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rather than to the module maintainer directly. Many experienced and |
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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=head2 Reporting Bugs |
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Report bugs to the Bioperl bug tracking system to help us keep track |
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the bugs and their resolution. Bug reports can be submitted via the |
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web: |
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https://github.com/bioperl/bioperl-live/issues |
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81
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=head1 AUTHOR - Aaron Mackey, Jason Stajich |
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Email amackey@virginia.edu |
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Email jason@bioperl.org |
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86
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=head1 APPENDIX |
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The rest of the documentation details each of the object |
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methods. Internal methods are usually preceded with a _ |
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=cut |
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93
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# Let the code begin... |
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package Bio::DB::GenPept; |
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use strict; |
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use vars qw($DEFAULTFORMAT $DEFAULTMODE %PARAMSTRING); |
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use base qw(Bio::DB::NCBIHelper); |
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105
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BEGIN { |
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$DEFAULTMODE = 'single'; |
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$DEFAULTFORMAT = 'gp'; |
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%PARAMSTRING = ( |
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'batch' => { 'db' => 'protein', |
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'usehistory' => 'n', |
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'tool' => 'bioperl'}, |
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# no query? |
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'gi' => { 'db' => 'protein', |
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'usehistory' => 'n', |
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'tool' => 'bioperl', |
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'retmode' => 'text'}, |
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'version' => { 'db' => 'protein', |
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'usehistory' => 'n', |
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'tool' => 'bioperl', |
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'retmode' => 'text'}, |
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'single' => { 'db' => 'protein', |
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'usehistory' => 'n', |
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'tool' => 'bioperl', |
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'retmode' => 'text'}, |
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'webenv' => { |
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'query_key' => 'querykey', |
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'WebEnv' => 'cookie', |
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'db' => 'protein', |
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'usehistory' => 'n', |
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'tool' => 'bioperl', |
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'retmode' => 'text'}, |
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); |
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} |
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# the new way to make modules a little more lightweight |
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sub new { |
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my($class, @args) = @_; |
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my $self = $class->SUPER::new(@args); |
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$self->request_format($self->default_format); |
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return $self; |
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} |
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=head2 get_params |
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Title : get_params |
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Usage : my %params = $self->get_params($mode) |
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Function: Returns key,value pairs to be passed to NCBI database |
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for either 'batch' or 'single' sequence retrieval method |
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Returns : a key,value pair hash |
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Args : 'single' or 'batch' mode for retrieval |
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=cut |
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sub get_params { |
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my ($self, $mode) = @_; |
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return defined $PARAMSTRING{$mode} ? %{$PARAMSTRING{$mode}} : %{$PARAMSTRING{$DEFAULTMODE}}; |
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} |
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=head2 default_format |
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156
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Title : default_format |
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Usage : my $format = $self->default_format |
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Function: Returns default sequence format for this module |
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Returns : string |
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Args : none |
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162
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=cut |
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164
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sub default_format { |
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return $DEFAULTFORMAT; |
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} |
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168
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# from Bio::DB::WebDBSeqI from Bio::DB::RandomAccessI |
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170
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=head1 Routines from Bio::DB::WebDBSeqI and Bio::DB::RandomAccessI |
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172
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=head2 get_Seq_by_id |
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174
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Title : get_Seq_by_id |
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Usage : $seq = $db->get_Seq_by_id('ROA1_HUMAN') |
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Function: Gets a Bio::Seq object by its name |
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Returns : a Bio::Seq object |
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Args : the id (as a string) of a sequence |
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Throws : "id does not exist" exception |
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181
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=head2 get_Seq_by_acc |
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183
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Title : get_Seq_by_acc |
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Usage : $seq = $db->get_Seq_by_acc('AAC73346'); |
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185
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Function: Gets a Seq objects by accession number |
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186
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Returns : Bio::Seq object |
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187
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Args : accession number to retrive by |
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188
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189
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=head1 Routines implemented by Bio::DB::NCBIHelper |
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191
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=head2 get_request |
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192
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193
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Title : get_request |
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194
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Usage : my $url = $self->get_request |
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195
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Function: HTTP::Request |
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196
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Returns : |
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197
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Args : %qualifiers = a hash of qualifiers (ids, format, etc) |
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198
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199
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=head2 get_Stream_by_id |
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200
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201
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Title : get_Stream_by_id |
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202
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Usage : $stream = $db->get_Stream_by_id( [$uid1, $uid2] ); |
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203
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Function: Gets a series of Seq objects by unique identifiers |
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Returns : a Bio::SeqIO stream object |
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205
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Args : $ref : a reference to an array of unique identifiers for |
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206
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the desired sequence entries |
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207
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208
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=head2 get_Stream_by_acc (2) |
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209
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210
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Title : get_Stream_by_acc |
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Usage : $seq = $db->get_Stream_by_acc($acc); |
|
212
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|
|
|
|
Function: Gets a series of Seq objects by accession numbers |
|
213
|
|
|
|
|
|
|
Returns : a Bio::SeqIO stream object |
|
214
|
|
|
|
|
|
|
Args : $ref : a reference to an array of accession numbers for |
|
215
|
|
|
|
|
|
|
the desired sequence entries |
|
216
|
|
|
|
|
|
|
Note : For GenBank, this just calls the same code for get_Stream_by_id() |
|
217
|
|
|
|
|
|
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|
|
218
|
|
|
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|
|
|
=head2 request_format |
|
219
|
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|
|
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|
|
|
|
220
|
|
|
|
|
|
|
Title : request_format |
|
221
|
|
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|
|
|
|
Usage : my $format = $self->request_format; |
|
222
|
|
|
|
|
|
|
$self->request_format($format); |
|
223
|
|
|
|
|
|
|
Function: Get/Set sequence format retrieval |
|
224
|
|
|
|
|
|
|
Returns : string representing format |
|
225
|
|
|
|
|
|
|
Args : $format = sequence format |
|
226
|
|
|
|
|
|
|
|
|
227
|
|
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|
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|
|
=cut |
|
228
|
|
|
|
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|
|
|
|
229
|
|
|
|
|
|
|
# override to force format to be GenPept regardless |
|
230
|
|
|
|
|
|
|
sub request_format { |
|
231
|
0
|
|
|
0
|
1
|
|
my ($self) = @_; |
|
232
|
0
|
|
|
|
|
|
return $self->SUPER::request_format($self->default_format()); |
|
233
|
|
|
|
|
|
|
} |
|
234
|
|
|
|
|
|
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|
|
235
|
|
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|
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|
|
1; |
|
236
|
|
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|
|
__END__ |