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# BioPerl module for Bio::Tools::FootPrinter |
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# |
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# Please direct questions and support issues to |
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# |
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# Cared for by Shawn Hoon |
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# |
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# Copyright Shawn Hoon |
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# |
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# You may distribute this module under the same terms as perl itself |
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# POD documentation - main docs before the code |
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=head1 NAME |
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Bio::Tools::FootPrinter - write sequence features in FootPrinter format |
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=head1 SYNOPSIS |
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use Bio::Tools::FootPrinter; |
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my $tool = Bio::Tools::FootPrinter->new(-file=>"footprinter.out"); |
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while (my $result = $tool->next_feature){ |
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foreach my $feat($result->sub_SeqFeature){ |
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print $result->seq_id."\t".$feat->start."\t".$feat->end."\t".$feat->seq->seq."\n"; |
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} |
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} |
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=head1 DESCRIPTION |
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This module writes sequence features in FootPrinter format. |
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See L for more details. |
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=head1 FEEDBACK |
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=head2 Mailing Lists |
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User feedback is an integral part of the evolution of this and other |
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Bioperl modules. Send your comments and suggestions preferably to |
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the Bioperl mailing list. Your participation is much appreciated. |
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bioperl-l@bioperl.org - General discussion |
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http://bioperl.org/wiki/Mailing_lists - About the mailing lists |
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=head2 Support |
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Please direct usage questions or support issues to the mailing list: |
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I |
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rather than to the module maintainer directly. Many experienced and |
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reponsive experts will be able look at the problem and quickly |
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address it. Please include a thorough description of the problem |
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with code and data examples if at all possible. |
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=head2 Reporting Bugs |
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Report bugs to the Bioperl bug tracking system to help us keep track |
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of the bugs and their resolution. Bug reports can be submitted via the |
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web: |
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https://github.com/bioperl/bioperl-live/issues |
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=head1 AUTHOR - Shawn Hoon |
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Email shawnh@fugu-sg.org |
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=head1 APPENDIX |
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The rest of the documentation details each of the object methods. |
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Internal methods are usually preceded with a _ |
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=cut |
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# Let the code begin... |
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package Bio::Tools::FootPrinter; |
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use strict; |
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use Bio::SeqFeature::Generic; |
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use Bio::PrimarySeq; |
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use base qw(Bio::Root::Root Bio::Root::IO); |
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=head2 new |
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Title : new |
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Usage : my $obj = Bio::Tools::FootPrinter->new(); |
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Function: Builds a new Bio::Tools::FootPrinter object |
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Returns : Bio::Tools::FootPrinter |
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Args : -fh/-file => $val, # for initing input, see Bio::Root::IO |
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=cut |
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sub new { |
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my($class,@args) = @_; |
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my $self = $class->SUPER::new(@args); |
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$self->_initialize_io(@args); |
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return $self; |
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} |
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=head2 next_feature |
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Title : next_feature |
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Usage : my $r = $footprint->next_feature |
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Function: Get the next feature from parser data |
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Returns : L |
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Args : none |
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=cut |
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sub next_feature{ |
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my ($self) = @_; |
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$self->_parse_predictions() unless $self->_predictions_parsed(); |
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return shift @{$self->{'_feature'}}; |
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} |
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=head2 _add_feature |
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Title : _add_feature |
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Usage : $footprint->_add_feature($feat) |
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Function: Add feature to array |
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Returns : none |
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Args : none |
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=cut |
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sub _add_feature { |
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my ($self,$feat) = @_; |
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if($feat){ |
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push @{$self->{'_feature'}},$feat; |
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} |
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} |
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=head2 _parse_predictions |
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Title : _parse_predictions |
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Usage : my $r = $footprint->_parse_predictions |
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Function: do the parsing |
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Returns : none |
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Args : none |
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=cut |
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sub _parse_predictions { |
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my ($self) = @_; |
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$/=""; |
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my ($seq,$second,$third,$name); |
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while ($_ = $self->_readline) { |
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chomp; |
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my @array = split("\n",$_); |
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if ($#array == 5) { |
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# get rid of header |
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shift(@array); shift(@array); |
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} |
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if($#array == 3){ |
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if($name){ |
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$name=~s/>//; |
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my $feat = $self->_parse($name,$seq,$second,$third); |
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$self->_add_feature($feat); |
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} |
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$name = shift @array; |
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$seq = $array[0]; |
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$second = $array[1]; |
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$third = $array[2]; |
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next; |
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} |
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$seq .= $array[0]; |
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$third .= $array[2]; |
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} |
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$seq || return; |
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$name=~s/>//; |
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my $feat = $self->_parse($name,$seq,$second,$third); |
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$self->_add_feature($feat); |
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$self->_predictions_parsed(1); |
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} |
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=head2 _predictions_parsed |
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188
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Title : _predictions_parsed |
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Usage : $footprint->_predictions_parsed(1) |
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Function: Get/Set for whether predictions parsed |
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Returns : 1/0 |
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Args : none |
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194
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=cut |
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sub _predictions_parsed { |
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my ($self,$val) = @_; |
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if($val){ |
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$self->{'_predictions_parsed'} = $val; |
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} |
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return $self->{'_predictions_parsed'}; |
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} |
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=head2 _parse |
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207
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Title : _parse |
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Usage : $footprint->_parse($name,$seq,$pattern) |
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Function: do the actual parsing |
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Returns : L |
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Args : none |
212
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=cut |
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215
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sub _parse { |
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my ($self,$name,$seq,$score,$pattern) = @_; |
217
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my @char = split('',$pattern); |
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my @score = split('',$score); |
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220
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5
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my ($prev,$word,@words,@word_scores,$word_score); |
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my $i = 0; |
223
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6
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11
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for my $c ( @char ) { |
224
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4702
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100
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5132
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if( ! $word) { |
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225
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$word .= $c; |
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$prev = $c; |
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defined $score[$i] && |
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|
|
|
($score[$i] =~ /\d/) && ($word_score += $score[$i]); |
229
|
|
|
|
|
|
|
} elsif ($c eq $prev){ |
230
|
4677
|
|
|
|
|
2555
|
$word .=$c; |
231
|
4677
|
|
|
|
|
2719
|
$prev = $c; |
232
|
4677
|
50
|
66
|
|
|
6395
|
defined $score[$i] && |
233
|
|
|
|
|
|
|
($score[$i] =~ /\d/) && ($word_score += $score[$i]); |
234
|
|
|
|
|
|
|
} else { |
235
|
|
|
|
|
|
|
# remove words with only \s |
236
|
19
|
|
|
|
|
48
|
$word=~s/\s+//g; |
237
|
19
|
100
|
|
|
|
30
|
if ($word ne ''){ |
238
|
12
|
|
|
|
|
19
|
push @words, $word; |
239
|
12
|
|
|
|
|
15
|
push @word_scores, ($word_score/length($word)); |
240
|
|
|
|
|
|
|
} |
241
|
19
|
|
|
|
|
15
|
$word =$c; |
242
|
19
|
|
|
|
|
10
|
$prev = $c; |
243
|
19
|
|
|
|
|
18
|
$word_score = 0; |
244
|
19
|
50
|
33
|
|
|
30
|
defined $score[$i] && |
245
|
|
|
|
|
|
|
($score[$i] =~ /\d/) && ($word_score += $score[$i]); |
246
|
|
|
|
|
|
|
} |
247
|
4702
|
|
|
|
|
3095
|
$i++; |
248
|
|
|
|
|
|
|
} |
249
|
6
|
|
|
|
|
26
|
$word =~s/\s+//g; |
250
|
6
|
50
|
|
|
|
14
|
if( length($word) ){ |
251
|
0
|
|
|
|
|
0
|
push @words, $word; |
252
|
|
|
|
|
|
|
} |
253
|
6
|
|
|
|
|
4
|
my $last; |
254
|
6
|
|
|
|
|
30
|
my $feat = Bio::SeqFeature::Generic->new(-seq_id=>$name); |
255
|
6
|
|
|
|
|
7
|
my $offset = $i = 0; |
256
|
6
|
|
|
|
|
4
|
my $count = 1; |
257
|
6
|
|
|
|
|
8
|
for my $w (@words){ |
258
|
12
|
50
|
|
|
|
18
|
if(length($w) ) { |
259
|
12
|
|
|
|
|
19
|
my $index = index($pattern,$w,$offset); |
260
|
12
|
|
|
|
|
10
|
$offset = $index + length($w); |
261
|
12
|
|
|
|
|
55
|
my $subfeat = Bio::SeqFeature::Generic->new |
262
|
|
|
|
|
|
|
( -seq_id =>"$name-motif".$count++, |
263
|
|
|
|
|
|
|
-start => $index+1, |
264
|
|
|
|
|
|
|
-end => $index+length($w), |
265
|
|
|
|
|
|
|
-source =>"FootPrinter", |
266
|
|
|
|
|
|
|
-score => $word_scores[$i] |
267
|
|
|
|
|
|
|
); |
268
|
|
|
|
|
|
|
# ugh - not sure the sub_SeqFeature situation will |
269
|
|
|
|
|
|
|
# be around forever- things should probably be |
270
|
|
|
|
|
|
|
# grouped by a 'group' tag instead ala GFF3 |
271
|
|
|
|
|
|
|
# perhaps when Lincoln's API changes are |
272
|
|
|
|
|
|
|
# made to SeqFeatures this will get changed |
273
|
12
|
|
|
|
|
26
|
$feat->add_sub_SeqFeature($subfeat,'EXPAND'); |
274
|
|
|
|
|
|
|
} |
275
|
12
|
|
|
|
|
14
|
$i++; |
276
|
|
|
|
|
|
|
} |
277
|
6
|
|
|
|
|
25
|
my $priseq = Bio::PrimarySeq->new(-id=>$name,-seq=>$seq); |
278
|
6
|
|
|
|
|
14
|
$feat->attach_seq($priseq); |
279
|
6
|
|
|
|
|
192
|
return $feat; |
280
|
|
|
|
|
|
|
|
281
|
|
|
|
|
|
|
} |
282
|
|
|
|
|
|
|
|
283
|
|
|
|
|
|
|
1; |