File Coverage

blib/lib/Bio/Polloc/Typing/bandingPattern.pm

Criterion	Covered	Total	%
statement	20	82	24.3
branch	6	22	27.2
condition	0	17	0.0
subroutine	5	11	45.4
pod	7	7	100.0
total	38	139	27.3

=head1 NAME

Bio::Polloc::Typing::bandingPattern - banding-pattern-based methods
for typing assessment

=head1 DESCRIPTION

Category 1 of genotyping methods in:

  Li, W., Raoult, D., & Fournier, P.-E. (2009).
  Bacterial strain typing in the genomic era.
  FEMS Microbiology Reviews, 33(5), 892-916.

=head1 AUTHOR - Luis M. Rodriguez-R

Email lmrodriguezr at gmail dot com

=cut

package Bio::Polloc::Typing::bandingPattern;
use base qw(Bio::Polloc::TypingI);
use strict;
our $VERSION = 1.0503; # [a-version] from Bio::Polloc::Polloc::Version


=head1 APPENDIX

Methods provided by the package

=head2 new

Generic initialization method

=cut

sub new {
   my($caller,@args) = @_;
   my $self = $caller->SUPER::new(@args);
   $self->_initialize(@args);
   return $self;
}

=head1 METHODS FROM Bio::Polloc::TypingI

=head2 scan

See L<Bio::Polloc::TypingI-E<gt>scan>.

L<fragments> must be implemented by the C<Bio::Polloc::Typing::bandingPattern::*>
object.

=cut

sub scan {
   my ($self, @args) = @_;
   my ($locigroup) = $self->_rearrange([qw(LOCIGROUP)], @args);
   $locigroup ||= $self->locigroup;
   return $self->_scan_locigroup($self->fragments(-locigroup=>$locigroup));
}

=head2 cluster

=head2 typing_value

See L<Bio::Polloc::TypingI-E<gt>typing_value>.

Returns the size of the loci between the minimum (L<min_size>)
and the maximum (L<max_size>) size.

=head3 Returns

A reference to an array of integers.

=cut

sub typing_value {
   my($self, @args) = @_;
   my($loci) = $self->_rearrange([qw(LOCI)], @args);
   $self->throw("Impossible to analyze loci", $loci)
   	unless defined $loci and ref($loci) and ref($loci)=~/ARRAY/i;
   my $out = [];
   for my $l (@$loci){
      my $size = abs($l->to - $l->from);
      push @$out, $size if $size<=$self->max_size and $size>=$self->min_size;
   }
   return $out;
}

=head2 graph_content

Generates the expected gel.  See L<Bio::Polloc::TypingI-E<gt>graph>.

=cut

sub graph_content {
   my($self, $locigroup, $width, $height, $font) = @_;
   return unless defined $locigroup;
   return unless $self->_load_module('GD::Simple');

   # Prepare data
   my $struc = $locigroup->structured_loci;
   my $genomes = $locigroup->genomes;
   $self->throw("You must define the genomes of the loci group in order to create a gel")
   	unless defined $genomes;

   # Set the gel up
   my $below = 50;
   my($iw, $ih, $nameh, $maxa) = ($width, $height-$below, 75, $self->max_size);
   $maxa = 5e3 if $maxa > 5e10;
   my($lw, $lh, $nh) = ($iw/($#$genomes+1), int($maxa/750), ($ih-$nameh)/$maxa);
   my $img = GD::Simple->new($width, $height);
   $img->bgcolor('black');
   $img->fgcolor('black');
   $img->rectangle(0, $nameh, $iw, $ih+$below);
   $img->font($font);
   my $white = $img->alphaColor(255,255,255,0);
   my $b1 = $img->alphaColor(130, 130, 130 ,0);
   $self->debug("GEL iw:$iw ih:$ih nameh:$nameh maxa:$maxa lw:$lw lh:$lh nh:$nh");

   # Draw bands
   for my $g (0 .. $#$struc){
      $img->fgcolor('black');
      $img->moveTo(int($lw*($g+0.2)), int($nameh*0.9));
      $img->fontsize(8);
      $img->angle(-45);
      $img->string($genomes->[$g]->name);
      $img->angle(0);
      my $x1 = int($lw*($g+0.1));
      my $x2 = $x1+int($lw*0.8);
      $self->debug("Lane from $x1 to $x2");
      for my $l (@{$struc->[$g]}){
	 $self->throw("Bad loci structure (g:$g)", $struc->[$g], "Bio::Polloc::Polloc::UnexpectedException")
	 	unless UNIVERSAL::can($l, 'isa') and $l->isa('Bio::Polloc::LocusI');
	 my $y1 = $nameh + int($nh*($maxa - $l->length));
	 my $y2 = $y1 + $lh;
	 my $S = ($l->score || 100) * 255 / 100;
	 my $b1 = $img->alphaColor($S, $S, $S, 0);
	 my $b2 = $img->alphaColor($S/2, $S/2, $S/2, 0);
	 $img->bgcolor($b2);
	 $img->fgcolor($b2);
	 $img->rectangle($x1, $y1-int($lh*0.75), $x2, $y2+int($lh*0.75));
	 $img->bgcolor($b1);
	 $img->fgcolor($b1);
	 $img->rectangle($x1, $y1, $x2, $y2);
	 $self->debug("Band from $y1 to $y2");
      }
   }
   return $img;
}


=head1 SPECIFIC METHODS

=head2 fragments

Generates fragments.

=head3 Arguments

=over

=item -locigroup I<Bio::Polloc::LociGroup>

The group of loci to be used as base to design the protocol.

=back

=head3 Returns

A L<Bio::Polloc::LociGrop>, where each locus is a fragment.

=head3 Throws

A L<Bio::Polloc::Polloc::NotImplementedException> unless implemented
by the specific C<Bio::Polloc::Typing::bandingPattern::*> object.

=cut

sub fragments { $_[0]->throw("fragments", $_[0], "Bio::Polloc::Polloc::NotImplementedException") }

=head2 max_size

Gets/sets the maximum locus size.  No limit (C<inf>) by default.

=cut

sub max_size {
   my($self, $value) = @_;
   $self->{'_max_size'} = $value+0 if defined $value;
   $self->{'_max_size'} = 0 + "Inf" unless defined $self->{'_max_size'};
   return $self->{'_max_size'};
}

=head2 min_size

Gets/sets the minimum locus size.  1 by default.

=cut

sub min_size {
   my($self, $value) = @_;
   $self->{'_min_size'} = $value+0 if defined $value;
   $self->{'_min_size'} = 1 unless defined $self->{'_min_size'};
   return $self->{'_min_size'};
}

=head1 INTERNAL METHODS

Methods intended to be used only within the scope of Bio::Polloc::*

=head2 _initialize

=cut

sub _initialize {
   my($self,@args) = @_;
   my($minSize, $maxSize) = $self->_rearrange([qw(MINSIZE MAXSIZE)], @args);
   $self->type('bandingPattern');
   $self->min_size($minSize);
   $self->max_size($maxSize);
   $self->_initialize_method(@args);
}

=head2 _initialize_method

=cut

sub _initialize_method { }

1;