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package Bio::VertRes::Config::Pipelines::Mapping; |
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# ABSTRACT: The base class for the mapping pipeline. |
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133198
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use Moose; |
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use Bio::VertRes::Config::Pipelines::Common; |
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use Bio::VertRes::Config::References; |
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use Bio::VertRes::Config::Pipelines::Roles::MetaDataFilter; |
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use Bio::VertRes::Config::Pipelines::Roles::MultiplePrefix; |
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extends 'Bio::VertRes::Config::Pipelines::Common'; |
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with 'Bio::VertRes::Config::Pipelines::Roles::MetaDataFilter'; |
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with 'Bio::VertRes::Config::Pipelines::Roles::MultiplePrefix'; |
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has 'pipeline_short_name' => ( is => 'ro', isa => 'Str', default => 'mapping' ); |
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has 'module' => ( is => 'ro', isa => 'Str', default => 'VertRes::Pipelines::Mapping' ); |
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has 'reference' => ( is => 'ro', isa => 'Str', required => 1 ); |
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has 'reference_lookup_file' => ( is => 'ro', isa => 'Str', required => 1 ); |
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has 'toplevel_action' => ( is => 'ro', isa => 'Str', default => '__VRTrack_Mapping__' ); |
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has 'slx_mapper' => ( is => 'ro', isa => 'Str', required => 1 ); |
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has 'slx_mapper_exe' => ( is => 'ro', isa => 'Str', required => 1 ); |
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has '_reference_fasta' => ( is => 'ro', isa => 'Str', lazy => 1, builder => '_build__reference_fasta'); |
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has '_mark_duplicates' => ( is => 'ro', isa => 'Int', default => 1 ); |
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has '_do_cleanup' => ( is => 'ro', isa => 'Int', default => 1 ); |
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has '_do_recalibration' => ( is => 'ro', isa => 'Int', default => 0 ); |
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has '_exit_on_errors' => ( is => 'ro', isa => 'Int', default => 0 ); |
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has '_get_genome_coverage' => ( is => 'ro', isa => 'Int', default => 1 ); |
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has '_add_index' => ( is => 'ro', isa => 'Int', default => 1 ); |
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has '_ignore_mapped_status' => ( is => 'ro', isa => 'Int', default => 1 ); |
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has '_dont_use_get_lanes' => ( is => 'ro', isa => 'Bool', default => 1 ); |
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sub _build__reference_fasta { |
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my ($self) = @_; |
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Bio::VertRes::Config::References->new( reference_lookup_file => $self->reference_lookup_file ) |
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->get_reference_location_on_disk( $self->reference ); |
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} |
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sub _construct_filename |
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{ |
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my ($self, $suffix) = @_; |
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my $output_filename = $self->_limits_values_part_of_filename(); |
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$output_filename = join( '_', ($output_filename, $self->reference, $self->slx_mapper ) ); |
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return $self->_filter_characters_truncate_and_add_suffix($output_filename,$suffix); |
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} |
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override 'log_file_name' => sub { |
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my ($self) = @_; |
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return $self->_construct_filename('log'); |
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}; |
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override 'config_file_name' => sub { |
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my ($self) = @_; |
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return $self->_construct_filename('conf'); |
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}; |
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override 'to_hash' => sub { |
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my ($self) = @_; |
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my $output_hash = super(); |
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$output_hash->{vrtrack_processed_flags} = { import => 1, qc => 1, stored => 1 }; |
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$output_hash->{limits} = $self->_escaped_limits; |
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$output_hash->{data}{mark_duplicates} = $self->_mark_duplicates; |
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$output_hash->{data}{reference} = $self->_reference_fasta; |
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$output_hash->{data}{assembly_name} = $self->reference; |
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$output_hash->{data}{do_cleanup} = $self->_do_cleanup; |
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$output_hash->{data}{do_recalibration} = $self->_do_recalibration; |
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$output_hash->{data}{exit_on_errors} = $self->_exit_on_errors; |
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$output_hash->{data}{get_genome_coverage} = $self->_get_genome_coverage; |
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$output_hash->{data}{add_index} = $self->_add_index; |
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$output_hash->{data}{ignore_mapped_status} = $self->_ignore_mapped_status; |
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$output_hash->{data}{slx_mapper} = $self->slx_mapper; |
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$output_hash->{data}{slx_mapper_exe} = $self->slx_mapper_exe; |
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$output_hash->{dont_use_get_lanes} = $self->_dont_use_get_lanes; |
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return $output_hash; |
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}; |
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__PACKAGE__->meta->make_immutable; |
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no Moose; |
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1; |
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__END__ |
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=pod |
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=head1 NAME |
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Bio::VertRes::Config::Pipelines::Mapping - The base class for the mapping pipeline. |
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=head1 VERSION |
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version 1.133090 |
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=head1 SYNOPSIS |
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The base class for the mapping pipeline. It wont produce useable output on its own, you need to call a sub classed mapper for that. |
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use Bio::VertRes::Config::Pipelines::Mapping; |
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my $pipeline = Bio::VertRes::Config::Pipelines::Mapping->new( |
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database => 'abc', |
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reference => 'Staphylococcus_aureus_subsp_aureus_ABC_v1', |
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limits => { |
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project => ['ABC study'], |
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species => ['EFG'] |
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}, |
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slx_mapper => 'bwa', |
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slx_mapper_exe => '/path/to/mapper/mapper.exe' |
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); |
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$pipeline->to_hash(); |
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=head1 AUTHOR |
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Andrew J. Page <ap13@sanger.ac.uk> |
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=head1 COPYRIGHT AND LICENSE |
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This software is Copyright (c) 2013 by Wellcome Trust Sanger Institute. |
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This is free software, licensed under: |
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The GNU General Public License, Version 3, June 2007 |
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=cut |