line |
stmt |
bran |
cond |
sub |
pod |
time |
code |
1
|
|
|
|
|
|
|
package Bio::VertRes::Config::Pipelines::BamImprovement; |
2
|
|
|
|
|
|
|
|
3
|
|
|
|
|
|
|
# ABSTRACT: The base class for the bam improvement pipeline. |
4
|
|
|
|
|
|
|
|
5
|
|
|
|
|
|
|
|
6
|
1
|
|
|
1
|
|
132268
|
use Moose; |
|
0
|
|
|
|
|
|
|
|
0
|
|
|
|
|
|
|
7
|
|
|
|
|
|
|
use Bio::VertRes::Config::Pipelines::Common; |
8
|
|
|
|
|
|
|
use Bio::VertRes::Config::References; |
9
|
|
|
|
|
|
|
use Bio::VertRes::Config::Pipelines::Roles::MetaDataFilter; |
10
|
|
|
|
|
|
|
use Bio::VertRes::Config::Pipelines::Roles::MultiplePrefix; |
11
|
|
|
|
|
|
|
extends 'Bio::VertRes::Config::Pipelines::Common'; |
12
|
|
|
|
|
|
|
with 'Bio::VertRes::Config::Pipelines::Roles::MetaDataFilter'; |
13
|
|
|
|
|
|
|
with 'Bio::VertRes::Config::Pipelines::Roles::MultiplePrefix'; |
14
|
|
|
|
|
|
|
with 'Bio::VertRes::Config::Pipelines::Roles::FilenameWithReference'; |
15
|
|
|
|
|
|
|
|
16
|
|
|
|
|
|
|
has 'pipeline_short_name' => ( is => 'ro', isa => 'Str', default => 'improvement' ); |
17
|
|
|
|
|
|
|
has 'module' => ( is => 'ro', isa => 'Str', default => 'VertRes::Pipelines::BamImprovement::NonHuman' ); |
18
|
|
|
|
|
|
|
has 'reference' => ( is => 'ro', isa => 'Str', required => 1 ); |
19
|
|
|
|
|
|
|
has 'reference_lookup_file' => ( is => 'ro', isa => 'Str', required => 1); |
20
|
|
|
|
|
|
|
has 'toplevel_action' => ( is => 'ro', isa => 'Str', default => '__VRTrack_BamImprovement__' ); |
21
|
|
|
|
|
|
|
|
22
|
|
|
|
|
|
|
has 'slx_mapper' => ( is => 'ro', isa => 'Str', default => 'smalt' ); |
23
|
|
|
|
|
|
|
has '_reference_fasta' => ( is => 'ro', isa => 'Str', lazy => 1, builder => '_build__reference_fasta' ); |
24
|
|
|
|
|
|
|
has '_keep_original_bam_files' => ( is => 'ro', isa => 'Bool', default => 0 ); |
25
|
|
|
|
|
|
|
has '_ignore_bam_improvement_status' => ( is => 'ro', isa => 'Int', default => 1 ); |
26
|
|
|
|
|
|
|
|
27
|
|
|
|
|
|
|
sub _build__reference_fasta { |
28
|
|
|
|
|
|
|
my ($self) = @_; |
29
|
|
|
|
|
|
|
Bio::VertRes::Config::References->new( reference_lookup_file => $self->reference_lookup_file ) |
30
|
|
|
|
|
|
|
->get_reference_location_on_disk( $self->reference ); |
31
|
|
|
|
|
|
|
} |
32
|
|
|
|
|
|
|
|
33
|
|
|
|
|
|
|
override 'to_hash' => sub { |
34
|
|
|
|
|
|
|
my ($self) = @_; |
35
|
|
|
|
|
|
|
my $output_hash = super(); |
36
|
|
|
|
|
|
|
$output_hash->{limits} = $self->_escaped_limits; |
37
|
|
|
|
|
|
|
|
38
|
|
|
|
|
|
|
$output_hash->{vrtrack_processed_flags} = { import => 1, qc => 1, stored => 1, mapped => 1 }; |
39
|
|
|
|
|
|
|
$output_hash->{data}{reference} = $self->_reference_fasta; |
40
|
|
|
|
|
|
|
$output_hash->{data}{assembly_name} = $self->reference; |
41
|
|
|
|
|
|
|
$output_hash->{data}{slx_mapper} = $self->slx_mapper; |
42
|
|
|
|
|
|
|
$output_hash->{data}{keep_original_bam_files} = $self->_keep_original_bam_files; |
43
|
|
|
|
|
|
|
$output_hash->{data}{ignore_bam_improvement_status} = $self->_ignore_bam_improvement_status; |
44
|
|
|
|
|
|
|
return $output_hash; |
45
|
|
|
|
|
|
|
}; |
46
|
|
|
|
|
|
|
|
47
|
|
|
|
|
|
|
__PACKAGE__->meta->make_immutable; |
48
|
|
|
|
|
|
|
no Moose; |
49
|
|
|
|
|
|
|
1; |
50
|
|
|
|
|
|
|
|
51
|
|
|
|
|
|
|
__END__ |
52
|
|
|
|
|
|
|
|
53
|
|
|
|
|
|
|
=pod |
54
|
|
|
|
|
|
|
|
55
|
|
|
|
|
|
|
=head1 NAME |
56
|
|
|
|
|
|
|
|
57
|
|
|
|
|
|
|
Bio::VertRes::Config::Pipelines::BamImprovement - The base class for the bam improvement pipeline. |
58
|
|
|
|
|
|
|
|
59
|
|
|
|
|
|
|
=head1 VERSION |
60
|
|
|
|
|
|
|
|
61
|
|
|
|
|
|
|
version 1.133090 |
62
|
|
|
|
|
|
|
|
63
|
|
|
|
|
|
|
=head1 SYNOPSIS |
64
|
|
|
|
|
|
|
|
65
|
|
|
|
|
|
|
The base class for the bam improvement pipeline. |
66
|
|
|
|
|
|
|
use Bio::VertRes::Config::Pipelines::BamImprovement; |
67
|
|
|
|
|
|
|
|
68
|
|
|
|
|
|
|
my $pipeline = Bio::VertRes::Config::Pipelines::BamImprovement->new( |
69
|
|
|
|
|
|
|
database => 'abc', |
70
|
|
|
|
|
|
|
reference => 'Staphylococcus_aureus_subsp_aureus_ABC_v1', |
71
|
|
|
|
|
|
|
limits => { |
72
|
|
|
|
|
|
|
project => ['ABC study'], |
73
|
|
|
|
|
|
|
species => ['EFG'] |
74
|
|
|
|
|
|
|
} |
75
|
|
|
|
|
|
|
|
76
|
|
|
|
|
|
|
); |
77
|
|
|
|
|
|
|
$pipeline->to_hash(); |
78
|
|
|
|
|
|
|
|
79
|
|
|
|
|
|
|
=head1 AUTHOR |
80
|
|
|
|
|
|
|
|
81
|
|
|
|
|
|
|
Andrew J. Page <ap13@sanger.ac.uk> |
82
|
|
|
|
|
|
|
|
83
|
|
|
|
|
|
|
=head1 COPYRIGHT AND LICENSE |
84
|
|
|
|
|
|
|
|
85
|
|
|
|
|
|
|
This software is Copyright (c) 2013 by Wellcome Trust Sanger Institute. |
86
|
|
|
|
|
|
|
|
87
|
|
|
|
|
|
|
This is free software, licensed under: |
88
|
|
|
|
|
|
|
|
89
|
|
|
|
|
|
|
The GNU General Public License, Version 3, June 2007 |
90
|
|
|
|
|
|
|
|
91
|
|
|
|
|
|
|
=cut |