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package Bio::ToolBox::utility; |
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our $VERSION = '1.69'; |
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4
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=head1 NAME |
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6
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Bio::ToolBox::utility - common utility functions for Bio::ToolBox |
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8
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=head1 DESCRIPTION |
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These are general subroutines that don't fit in with the other modules. |
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12
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=head1 REGULAR SUBROUTINES |
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The following subroutines are automatically exported when you use this module. |
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16
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=over 4 |
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18
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=item parse_list |
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my $index_request = '1,2,5-7'; |
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my @indices = parse_list($index_request); # returns [1,2,5,6,7] |
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23
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This subroutine parses a scalar value into a list of values. The scalar is |
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a text string of numbers (usually column or dataset indices) delimited by |
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commas and/or including a range. For example, a string "1,2,5-7" would become |
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an array of [1,2,5,6,7]. |
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Pass the module the scalar string. |
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30
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It will return the array of numbers. |
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31
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32
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=item format_with_commas |
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33
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34
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my $count = '4327908475'; |
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35
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printf " The final count was %s\n", format_with_commas($count); |
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36
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37
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This subroutine process a large number (e.g. 4327908475) into a human-friendly |
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38
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version with commas delimiting the thousands (4,327,908,475). |
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39
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40
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Pass the module a scalar string with a number value. |
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41
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42
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It will return a scalar value containing the formatted number. |
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43
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44
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=item ask_user_for_index |
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45
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46
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my @answers = ask_user_for_index($Data, 'Please enter 2 or more columns '); |
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47
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48
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This subroutine will present the list of column names from a L |
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49
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structure along with their numeric indexes to the user and prompt for one |
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50
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or more to be selected and entered. The function is smart enough to only print |
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51
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the list once (if it hasn't changed) so as not to annoy the user with repeated |
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52
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lists of header names when used more than once. A text prompt should be provided, |
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53
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or a generic one is used. The list of indices are validated, and a warning printed |
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54
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for invalid responses. The responses are then returned as a single value or array, |
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55
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depending on context. |
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56
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57
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=item simplify_dataset_name |
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58
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59
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my $simple_name = simplify_dataset_name($dataset); |
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60
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61
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This subroutine will take a dataset name and simplify it. Dataset names may |
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62
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often be file names of data files, such as Bam and bigWig files. These may |
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63
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include a C, C, or C prefix, one or more directory paths, |
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64
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and one or more file name extensions. Additionally, more than one dataset |
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65
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may be combined, for example two stranded bigWig files, with an ampersand. |
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66
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This function will safely remove the prefix, directories, and everything after |
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67
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the first period. |
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68
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69
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=item sane_chromo_sort |
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70
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71
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my @chromo = $db->seq_ids; |
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72
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my @sorted = sane_chromo_sort(@chromo); |
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73
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74
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This subroutine will take a list of chromosome or sequence identifiers and sort |
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75
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them into a reasonably sane order: standard numeric identifiers first (numeric |
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76
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order), sex chromosomes (alphabetical), mitochondrial, names with text and |
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77
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numbers (text first alphabetically, then numbers numerically) for contigs and |
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78
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such, and finally anything else (aciibetically). Any 'chr' prefix is ignored. |
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79
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Roman numerals are properly handled numerically. |
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80
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81
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The provided list may be a list of SCALAR values (chromosome names) or ARRAY |
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82
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references, with the first element assumed to be the name, e.g. |
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83
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C<[$name, $length]>. |
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84
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85
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=back |
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86
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87
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=cut |
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88
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89
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4
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4
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658
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use strict; |
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4
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7
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4
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94
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90
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4
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4
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14
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use Carp; |
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4
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7
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4
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148
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91
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4
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4
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23
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use File::Spec; |
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4
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5
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4
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5568
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92
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require Exporter; |
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93
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94
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95
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### Variables |
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96
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# Export |
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97
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our @ISA = qw(Exporter); |
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98
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our @EXPORT = qw( |
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99
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parse_list |
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100
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format_with_commas |
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101
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ask_user_for_index |
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102
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simplify_dataset_name |
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103
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sane_chromo_sort |
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104
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); |
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105
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our $DATA_COLNAMES = undef; |
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106
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our $DATA_FILENAME = undef; |
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107
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108
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### The True Statement |
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109
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1; |
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110
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111
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112
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113
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################# The Subroutines ################### |
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114
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115
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### Parse string into list |
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116
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sub parse_list { |
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117
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# this subroutine will parse a string into an array |
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118
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# it is designed for a string of numbers delimited by commas |
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119
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# a range of numbers may be specified using a dash |
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120
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# hence 1,2,5-7 would become an array of 1,2,5,6,7 |
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121
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122
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2
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2
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1
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984
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my $string = shift; |
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123
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2
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50
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7
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return unless defined $string; |
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124
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2
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50
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6
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if ($string =~ /[^\d,\-\s\&]/) { |
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125
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0
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0
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carp " the string contains characters that can't be parsed\n"; |
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126
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0
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0
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return; |
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127
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} |
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128
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2
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3
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my @list; |
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129
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2
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9
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foreach (split /[,\s+]/, $string) { |
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130
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# check for a range |
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131
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5
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100
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10
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if (/\-/) { |
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132
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2
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5
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my ($start, $stop) = split /\-/; |
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133
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# add each item in the range to the list |
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134
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2
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6
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for (my $i = $start; $i <= $stop; $i++) { |
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135
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13
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19
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push @list, $i; |
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136
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} |
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137
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2
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3
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next; |
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138
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} |
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139
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else { |
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140
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# either an ordinary number or an "&"ed list of numbers |
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141
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3
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4
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push @list, $_; |
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142
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} |
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143
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} |
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144
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2
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8
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return @list; |
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145
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} |
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146
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147
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148
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149
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### Format a number into readable comma-delimited by thousands number |
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150
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sub format_with_commas { |
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151
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# for formatting a number with commas |
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152
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3
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3
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1
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1459
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my $number = shift; |
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153
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154
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# check number |
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155
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3
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4
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my ($integers, $decimals, $sign); |
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156
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3
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100
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28
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if ($number =~ /^(\-)?(\d+)\.(\d+)$/) { |
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50
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157
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2
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5
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$sign = $1; |
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158
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2
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3
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$integers = $2; |
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159
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2
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4
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$decimals = $3; |
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160
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} |
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161
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elsif ($number =~ /^(\-)?(\d+)$/) { |
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162
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1
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3
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$sign = $1; |
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163
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1
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2
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$integers = $2; |
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164
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} |
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165
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else { |
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166
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0
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0
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carp " the string contains characters that can't be parsed\n"; |
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167
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0
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0
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return $number; |
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168
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} |
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169
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170
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# format |
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171
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3
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10
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my @digits = split //, $integers; |
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172
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3
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3
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my @formatted; |
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173
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3
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7
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while (@digits) { |
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174
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10
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100
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15
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if (@digits > 3) { |
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175
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7
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12
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unshift @formatted, pop @digits; |
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176
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7
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10
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unshift @formatted, pop @digits; |
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177
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7
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9
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unshift @formatted, pop @digits; |
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178
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7
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10
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unshift @formatted, ','; |
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179
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} |
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180
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else { |
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181
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3
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6
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while (@digits) { |
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182
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4
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9
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unshift @formatted, pop @digits; |
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183
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} |
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184
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} |
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185
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} |
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186
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187
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# finished |
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188
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3
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100
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4
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my $final = $sign ? $sign : ''; |
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189
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3
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7
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$final .= join("", @formatted); |
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190
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3
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100
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7
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$final .= '.' . $decimals if defined $decimals; |
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191
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3
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15
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return $final; |
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192
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} |
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193
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194
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195
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sub ask_user_for_index { |
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196
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0
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0
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1
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0
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my $Data = shift; |
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197
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0
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0
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0
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my $line = shift || ' Enter the desired column index '; |
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198
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0
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0
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0
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unless (ref($Data) =~ /Bio::ToolBox::Data/) { |
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199
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0
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0
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carp "Must pass a Bio::ToolBox::Data object!\n"; |
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200
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0
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0
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return; |
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201
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} |
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202
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203
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# print column header names only if we have not done so before |
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204
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0
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0
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0
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0
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unless ( |
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205
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# we use filename and column number as indicators |
|
206
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$Data->filename eq $DATA_FILENAME and |
|
207
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join(";", $Data->list_columns) eq $DATA_COLNAMES |
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208
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) { |
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209
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0
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0
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print " These are the columns in the file\n"; |
|
210
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0
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0
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my $i = 0; |
|
211
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0
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0
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foreach ($Data->list_columns) { |
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212
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0
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0
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print " $i\t$_\n"; |
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213
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0
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0
|
$i++; |
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214
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} |
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215
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# remember for next time |
|
216
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0
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0
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$DATA_FILENAME = $Data->filename; |
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217
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0
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0
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$DATA_COLNAMES = join(";", $Data->list_columns); |
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218
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} |
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219
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0
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0
|
print $line; |
|
220
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221
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# get response |
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222
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0
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0
|
my $response = ; |
|
223
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0
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0
|
chomp $response; |
|
224
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0
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0
|
my @indices = parse_list($response); |
|
225
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226
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# verify |
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227
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0
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0
|
my @good; |
|
228
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0
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0
|
foreach (@indices) { |
|
229
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0
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0
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0
|
if ($Data->name($_)) { |
|
230
|
0
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0
|
push @good, $_; |
|
231
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} |
|
232
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else { |
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233
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0
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0
|
print " $_ is not a valid index!\n"; |
|
234
|
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|
} |
|
235
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} |
|
236
|
0
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0
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|
0
|
return wantarray ? @good : $good[0]; |
|
237
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|
} |
|
238
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|
239
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|
240
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|
|
sub simplify_dataset_name { |
|
241
|
14
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|
14
|
1
|
4665
|
my $dataset = shift; |
|
242
|
14
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|
17
|
my $new_name; |
|
243
|
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|
244
|
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|
|
|
# strip any file prefix |
|
245
|
14
|
|
|
|
|
39
|
$dataset =~ s/^(?:file|http|ftp):\/*//; |
|
246
|
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|
247
|
14
|
100
|
|
|
|
30
|
if ($dataset =~ /&/) { |
|
248
|
|
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|
|
|
|
# a combination dataset |
|
249
|
2
|
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|
|
5
|
foreach (split /&/, $dataset) { |
|
250
|
4
|
|
|
|
|
9
|
my $n = simplify_dataset_name($_); |
|
251
|
4
|
100
|
|
|
|
10
|
if ($new_name) { |
|
252
|
2
|
|
|
|
|
4
|
$new_name .= '&' . $n; |
|
253
|
|
|
|
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|
|
} |
|
254
|
|
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|
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|
|
else { |
|
255
|
2
|
|
|
|
|
3
|
$new_name = $n; |
|
256
|
|
|
|
|
|
|
} |
|
257
|
|
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|
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|
|
} |
|
258
|
|
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|
|
} |
|
259
|
|
|
|
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|
|
else { |
|
260
|
|
|
|
|
|
|
# a single dataset |
|
261
|
|
|
|
|
|
|
# this could be either a file name or an entry in a BioPerl or BigWigSet database |
|
262
|
|
|
|
|
|
|
# remove any possible paths |
|
263
|
12
|
|
|
|
|
95
|
(undef, undef, $new_name) = File::Spec->splitpath($dataset); |
|
264
|
|
|
|
|
|
|
|
|
265
|
|
|
|
|
|
|
# remove any known file extensions |
|
266
|
12
|
|
|
|
|
44
|
$new_name =~ s/\.(?:bw|bam|bb|useq|bigwig|bigbed|g[tf]f3?|cram|wig|bdg|bedgraph)(?:\.gz)?$//i; |
|
267
|
|
|
|
|
|
|
|
|
268
|
|
|
|
|
|
|
# remove common non-useful stuff |
|
269
|
|
|
|
|
|
|
# trying to imagine all sorts of possible things |
|
270
|
12
|
|
|
|
|
27
|
$new_name =~ s/[_\.\-](?:sort|sorted|dedup|dedupe|deduplicated|rmdup|mkdup|markdup|dup|unique|filt|filtered)\b//gi; |
|
271
|
12
|
|
|
|
|
23
|
$new_name =~ s/[_\.\-](?:coverage|rpm|ext\d*|extend\d*|log2fe|log\d+|qvalue|fragment|count|lambda_control|fe|fold.?enrichment|ratio|log\d*ratio)\b//gi; |
|
272
|
|
|
|
|
|
|
} |
|
273
|
14
|
|
|
|
|
24
|
return $new_name; |
|
274
|
|
|
|
|
|
|
} |
|
275
|
|
|
|
|
|
|
|
|
276
|
|
|
|
|
|
|
|
|
277
|
|
|
|
|
|
|
sub sane_chromo_sort { |
|
278
|
4
|
|
|
4
|
1
|
1496
|
my @chroms = @_; |
|
279
|
4
|
50
|
|
|
|
11
|
return unless scalar @chroms; |
|
280
|
|
|
|
|
|
|
|
|
281
|
|
|
|
|
|
|
# let's try and sort in some kind of rational order |
|
282
|
4
|
|
|
|
|
17
|
my @numeric; |
|
283
|
|
|
|
|
|
|
my @romanic; |
|
284
|
4
|
|
|
|
|
0
|
my @mixed; |
|
285
|
4
|
|
|
|
|
0
|
my @alphic; |
|
286
|
4
|
|
|
|
|
0
|
my @sex; |
|
287
|
4
|
|
|
|
|
0
|
my @mito; |
|
288
|
4
|
|
|
|
|
7
|
foreach my $c (@chroms) { |
|
289
|
|
|
|
|
|
|
|
|
290
|
42
|
|
|
|
|
43
|
my $name; |
|
291
|
42
|
50
|
|
|
|
51
|
if (ref($c) eq 'ARRAY') { |
|
292
|
0
|
|
|
|
|
0
|
$name = $c->[0]; |
|
293
|
|
|
|
|
|
|
} |
|
294
|
|
|
|
|
|
|
else { |
|
295
|
42
|
|
|
|
|
39
|
$name = $c; |
|
296
|
|
|
|
|
|
|
} |
|
297
|
|
|
|
|
|
|
|
|
298
|
|
|
|
|
|
|
# identify the type of chromosome name to sort |
|
299
|
42
|
100
|
|
|
|
164
|
if ($name =~ /^(?:chr)?([wxyz])$/i) { |
|
|
|
100
|
|
|
|
|
|
|
|
|
100
|
|
|
|
|
|
|
|
|
100
|
|
|
|
|
|
|
|
|
100
|
|
|
|
|
|
|
300
|
|
|
|
|
|
|
# sex chromosomes |
|
301
|
4
|
|
|
|
|
10
|
push @sex, [$1, $c]; |
|
302
|
|
|
|
|
|
|
} |
|
303
|
|
|
|
|
|
|
elsif ($name =~ /^(?:chr)?(?:m|mt|mito)(?:dna)?$/i) { |
|
304
|
|
|
|
|
|
|
# mitochondrial |
|
305
|
3
|
|
|
|
|
6
|
push @mito, [$name, $c]; |
|
306
|
|
|
|
|
|
|
} |
|
307
|
|
|
|
|
|
|
elsif ($name =~ /^(?:chr)?(\d+)$/i) { |
|
308
|
|
|
|
|
|
|
# standard numeric chromosome |
|
309
|
22
|
|
|
|
|
46
|
push @numeric, [$1, $c]; |
|
310
|
|
|
|
|
|
|
} |
|
311
|
|
|
|
|
|
|
elsif ($name =~ /^(?:chr)?([IVX]+)$/) { |
|
312
|
|
|
|
|
|
|
# Roman numerals - silly Saccharomyces cerevisiae |
|
313
|
7
|
|
|
|
|
19
|
push @romanic, [$1, $c]; |
|
314
|
|
|
|
|
|
|
} |
|
315
|
|
|
|
|
|
|
elsif ($name =~ /^([a-zA-Z_\-\.]+)(\d+)/) { |
|
316
|
|
|
|
|
|
|
# presumed contigs and such? |
|
317
|
5
|
|
|
|
|
12
|
push @mixed, [$1, $2, $name, $c]; |
|
318
|
|
|
|
|
|
|
} |
|
319
|
|
|
|
|
|
|
else { |
|
320
|
|
|
|
|
|
|
# everything else |
|
321
|
1
|
|
|
|
|
4
|
push @alphic, [$name, $c]; |
|
322
|
|
|
|
|
|
|
} |
|
323
|
|
|
|
|
|
|
} |
|
324
|
|
|
|
|
|
|
|
|
325
|
|
|
|
|
|
|
# check romanic |
|
326
|
4
|
100
|
|
|
|
9
|
if (scalar @romanic) { |
|
327
|
|
|
|
|
|
|
# looks like we have romanic chromosomes |
|
328
|
2
|
100
|
|
|
|
6
|
if (scalar @sex) { |
|
329
|
|
|
|
|
|
|
# probably caught up chrX, unlikely WYZ |
|
330
|
1
|
|
|
|
|
3
|
my @x = grep { $sex[$_]->[0] =~ m/^X$/ } (0 .. $#sex); |
|
|
2
|
|
|
|
|
6
|
|
|
331
|
1
|
|
|
|
|
3
|
foreach (reverse @x) { |
|
332
|
|
|
|
|
|
|
# I'm assuming and hoping there's only one chrX found |
|
333
|
|
|
|
|
|
|
# but reverse the list, just in case - assuming grep returns in order |
|
334
|
1
|
|
|
|
|
3
|
push @romanic, ( splice(@sex, $_, 1) ); |
|
335
|
|
|
|
|
|
|
} |
|
336
|
|
|
|
|
|
|
} |
|
337
|
2
|
50
|
|
|
|
5
|
if (scalar @numeric) { |
|
338
|
|
|
|
|
|
|
# well, shoot, this is weird, mix of both numeric and romanic chromosomes? |
|
339
|
|
|
|
|
|
|
# just merge romanic with alphic and hope for the best |
|
340
|
0
|
|
|
|
|
0
|
push @alphic, @romanic; |
|
341
|
|
|
|
|
|
|
} |
|
342
|
|
|
|
|
|
|
else { |
|
343
|
|
|
|
|
|
|
# convert the romanic to numeric |
|
344
|
2
|
|
|
|
|
5
|
while (@romanic) { |
|
345
|
8
|
|
|
|
|
15
|
my $r = shift @romanic; |
|
346
|
8
|
|
|
|
|
11
|
my $c = $r->[0]; |
|
347
|
8
|
|
|
|
|
12
|
$c =~ s/IV/4/; |
|
348
|
8
|
|
|
|
|
13
|
$c =~ s/IX/9/; |
|
349
|
8
|
|
|
|
|
12
|
$c =~ s/V/5/; |
|
350
|
8
|
|
|
|
|
12
|
$c =~ s/I/1/g; |
|
351
|
8
|
|
|
|
|
12
|
my $n = 0; |
|
352
|
8
|
|
|
|
|
15
|
foreach (split q(), $c) { |
|
353
|
15
|
100
|
|
|
|
22
|
if ($_ eq 'X') { |
|
354
|
2
|
|
|
|
|
3
|
$n += 10; |
|
355
|
|
|
|
|
|
|
} |
|
356
|
|
|
|
|
|
|
else { |
|
357
|
13
|
|
|
|
|
16
|
$n += $_; |
|
358
|
|
|
|
|
|
|
} |
|
359
|
|
|
|
|
|
|
} |
|
360
|
8
|
|
|
|
|
44
|
push @numeric, [$n, $r->[1]]; |
|
361
|
|
|
|
|
|
|
} |
|
362
|
|
|
|
|
|
|
} |
|
363
|
|
|
|
|
|
|
} |
|
364
|
|
|
|
|
|
|
|
|
365
|
|
|
|
|
|
|
# sort |
|
366
|
4
|
|
|
|
|
5
|
my @sorted; |
|
367
|
4
|
|
|
|
|
16
|
push @sorted, map { $_->[1] } sort {$a->[0] <=> $b->[0]} @numeric; |
|
|
30
|
|
|
|
|
40
|
|
|
|
66
|
|
|
|
|
68
|
|
|
368
|
4
|
|
|
|
|
9
|
push @sorted, map { $_->[1] } sort {$a->[0] cmp $b->[0]} @sex; |
|
|
3
|
|
|
|
|
5
|
|
|
|
1
|
|
|
|
|
3
|
|
|
369
|
4
|
|
|
|
|
6
|
push @sorted, map { $_->[1] } sort {$a->[0] cmp $b->[0]} @mito; |
|
|
3
|
|
|
|
|
7
|
|
|
|
0
|
|
|
|
|
0
|
|
|
370
|
5
|
|
|
|
|
7
|
push @sorted, map { $_->[3] } sort { |
|
371
|
4
|
50
|
33
|
|
|
7
|
$a->[0] cmp $b->[0] or |
|
|
4
|
|
|
|
|
18
|
|
|
372
|
|
|
|
|
|
|
$a->[1] <=> $b->[1] or |
|
373
|
|
|
|
|
|
|
$a->[2] cmp $b->[2] |
|
374
|
|
|
|
|
|
|
} @mixed; |
|
375
|
4
|
|
|
|
|
7
|
push @sorted, map { $_->[1] } sort { $a->[0] cmp $b->[0] } @alphic; |
|
|
1
|
|
|
|
|
1
|
|
|
|
0
|
|
|
|
|
0
|
|
|
376
|
|
|
|
|
|
|
|
|
377
|
4
|
|
|
|
|
22
|
return @sorted; |
|
378
|
|
|
|
|
|
|
} |
|
379
|
|
|
|
|
|
|
|
|
380
|
|
|
|
|
|
|
|
|
381
|
|
|
|
|
|
|
__END__ |