line |
stmt |
bran |
cond |
sub |
pod |
time |
code |
1
|
|
|
|
|
|
|
package Bio::Roary::External::Fasttree; |
2
|
|
|
|
|
|
|
$Bio::Roary::External::Fasttree::VERSION = '3.11.0'; |
3
|
|
|
|
|
|
|
# ABSTRACT: Wrapper to run Fasttree |
4
|
|
|
|
|
|
|
|
5
|
|
|
|
|
|
|
|
6
|
1
|
|
|
1
|
|
6
|
use Moose; |
|
1
|
|
|
|
|
2
|
|
|
1
|
|
|
|
|
5
|
|
7
|
|
|
|
|
|
|
with 'Bio::Roary::JobRunner::Role'; |
8
|
|
|
|
|
|
|
|
9
|
|
|
|
|
|
|
has 'input_file' => ( is => 'ro', isa => 'Str', required => 1 ); |
10
|
|
|
|
|
|
|
has 'output_file' => ( is => 'ro', isa => 'Str', lazy => 1, builder => '_build_output_file' ); |
11
|
|
|
|
|
|
|
has 'exec' => ( is => 'ro', isa => 'Str', default => 'FastTree' ); |
12
|
|
|
|
|
|
|
has 'alt_exec' => ( is => 'ro', isa => 'Str', default => 'fasttree' ); |
13
|
|
|
|
|
|
|
has '_logging' => ( is => 'ro', isa => 'Str', default => '2> /dev/null' ); |
14
|
|
|
|
|
|
|
|
15
|
|
|
|
|
|
|
sub _build_output_file |
16
|
|
|
|
|
|
|
{ |
17
|
0
|
|
|
0
|
|
|
my ($self) = @_; |
18
|
0
|
|
|
|
|
|
return $self->input_file.".newick"; |
19
|
|
|
|
|
|
|
} |
20
|
|
|
|
|
|
|
|
21
|
|
|
|
|
|
|
sub _command_to_run { |
22
|
0
|
|
|
0
|
|
|
my ($self) = @_; |
23
|
|
|
|
|
|
|
|
24
|
0
|
|
|
|
|
|
my $executable = $self->_find_exe([$self->exec, $self->alt_exec]); |
25
|
0
|
|
|
|
|
|
my $logging_str = ""; |
26
|
0
|
0
|
|
|
|
|
$logging_str = $self->_logging if(! $self->verbose); |
27
|
|
|
|
|
|
|
|
28
|
0
|
|
|
|
|
|
return join( |
29
|
|
|
|
|
|
|
' ', ($executable, '-fastest', '-nt', $self->input_file, '>', $self->output_file, $logging_str) |
30
|
|
|
|
|
|
|
); |
31
|
|
|
|
|
|
|
} |
32
|
|
|
|
|
|
|
|
33
|
|
|
|
|
|
|
sub run { |
34
|
0
|
|
|
0
|
0
|
|
my ($self) = @_; |
35
|
0
|
|
|
|
|
|
my @commands_to_run; |
36
|
|
|
|
|
|
|
|
37
|
0
|
0
|
0
|
|
|
|
if(!defined($self->input_file) || ! ( -e $self->input_file)) |
38
|
|
|
|
|
|
|
{ |
39
|
0
|
|
|
|
|
|
$self->logger->error( "The input file is missing so not creating a tree" ); |
40
|
0
|
|
|
|
|
|
return 1; |
41
|
|
|
|
|
|
|
} |
42
|
|
|
|
|
|
|
|
43
|
0
|
0
|
|
|
|
|
if(-s $self->input_file < 5) |
44
|
|
|
|
|
|
|
{ |
45
|
0
|
|
|
|
|
|
$self->logger->info( "The input file is too small so not creating a tree" ); |
46
|
0
|
|
|
|
|
|
return 1; |
47
|
|
|
|
|
|
|
} |
48
|
|
|
|
|
|
|
|
49
|
0
|
|
|
|
|
|
push(@commands_to_run, $self->_command_to_run() ); |
50
|
0
|
|
|
|
|
|
$self->logger->info( "Running command: " . $self->_command_to_run() ); |
51
|
0
|
|
|
|
|
|
my $job_runner_obj = $self->_job_runner_class->new( commands_to_run => \@commands_to_run, memory_in_mb => $self->memory_in_mb, queue => $self->_queue, cpus => $self->cpus ); |
52
|
0
|
|
|
|
|
|
$job_runner_obj->run(); |
53
|
|
|
|
|
|
|
|
54
|
0
|
|
|
|
|
|
1; |
55
|
|
|
|
|
|
|
} |
56
|
|
|
|
|
|
|
|
57
|
1
|
|
|
1
|
|
5922
|
no Moose; |
|
1
|
|
|
|
|
2
|
|
|
1
|
|
|
|
|
4
|
|
58
|
|
|
|
|
|
|
__PACKAGE__->meta->make_immutable; |
59
|
|
|
|
|
|
|
|
60
|
|
|
|
|
|
|
1; |
61
|
|
|
|
|
|
|
|
62
|
|
|
|
|
|
|
__END__ |
63
|
|
|
|
|
|
|
|
64
|
|
|
|
|
|
|
=pod |
65
|
|
|
|
|
|
|
|
66
|
|
|
|
|
|
|
=encoding UTF-8 |
67
|
|
|
|
|
|
|
|
68
|
|
|
|
|
|
|
=head1 NAME |
69
|
|
|
|
|
|
|
|
70
|
|
|
|
|
|
|
Bio::Roary::External::Fasttree - Wrapper to run Fasttree |
71
|
|
|
|
|
|
|
|
72
|
|
|
|
|
|
|
=head1 VERSION |
73
|
|
|
|
|
|
|
|
74
|
|
|
|
|
|
|
version 3.11.0 |
75
|
|
|
|
|
|
|
|
76
|
|
|
|
|
|
|
=head1 SYNOPSIS |
77
|
|
|
|
|
|
|
|
78
|
|
|
|
|
|
|
Wrapper to run cd-hit |
79
|
|
|
|
|
|
|
use Bio::Roary::External::Fasttree; |
80
|
|
|
|
|
|
|
|
81
|
|
|
|
|
|
|
my $obj = Bio::Roary::External::Fasttree->new( |
82
|
|
|
|
|
|
|
input_file => 'abc.fa', |
83
|
|
|
|
|
|
|
exec => 'Fasttree', |
84
|
|
|
|
|
|
|
output_base => 'efg', |
85
|
|
|
|
|
|
|
); |
86
|
|
|
|
|
|
|
$obj->run; |
87
|
|
|
|
|
|
|
|
88
|
|
|
|
|
|
|
=head1 AUTHOR |
89
|
|
|
|
|
|
|
|
90
|
|
|
|
|
|
|
Andrew J. Page <ap13@sanger.ac.uk> |
91
|
|
|
|
|
|
|
|
92
|
|
|
|
|
|
|
=head1 COPYRIGHT AND LICENSE |
93
|
|
|
|
|
|
|
|
94
|
|
|
|
|
|
|
This software is Copyright (c) 2013 by Wellcome Trust Sanger Institute. |
95
|
|
|
|
|
|
|
|
96
|
|
|
|
|
|
|
This is free software, licensed under: |
97
|
|
|
|
|
|
|
|
98
|
|
|
|
|
|
|
The GNU General Public License, Version 3, June 2007 |
99
|
|
|
|
|
|
|
|
100
|
|
|
|
|
|
|
=cut |